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Q9SJL9

- XTH32_ARATH

UniProt

Q9SJL9 - XTH32_ARATH

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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 32

Gene
XTH32, At2g36870, T1J8.5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei118 – 1181Nucleophile By similarity
Active sitei122 – 1221Proton donor By similarity

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. cell wall macromolecule catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G36870-MONOMER.
ARA:GQT-1891-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 32 (EC:2.4.1.207)
Short name:
At-XTH32
Short name:
XTH-32
Gene namesi
Name:XTH32
Ordered Locus Names:At2g36870
ORF Names:T1J8.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G36870.

Subcellular locationi

Secretedcell wall Inferred. Secretedextracellular spaceapoplast Inferred

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Chaini25 – 299275Probable xyloglucan endotransglucosylase/hydrolase protein 32PRO_0000011832Add
BLAST

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9SJL9.
PRIDEiQ9SJL9.

Expressioni

Tissue specificityi

Predominantly expressed in stems.1 Publication

Gene expression databases

ArrayExpressiQ9SJL9.
GenevestigatoriQ9SJL9.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G36870.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SJL9.
SMRiQ9SJL9. Positions 27-297.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG317325.
HOGENOMiHOG000236368.
InParanoidiQ9SJL9.
KOiK08235.
OMAiLTPECWR.
PhylomeDBiQ9SJL9.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SJL9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGNSLISLLS IFHLLVLWGS SVNAYWPPSP GYWPSSKVGS LNFYKGFRNL    50
WGPQHQRMDQ NALTIWLDRT SGSGFKSVKP FRSGYFGANI KLQPGYTAGV 100
ITSLYLSNNE AHPGFHDEVD IEFLGTTFGK PYTLQTNVYI RGSGDGKIIG 150
REMKFRLWFD PTKDFHHYAI LWSPREIIFL VDDIPIRRYP KKSASTFPLR 200
PMWLYGSIWD ASSWATEDGK YKADYKYQPF TAKYTNFKAL GCTAYSSARC 250
YPLSASPYRS GGLTRQQHQA MRWVQTHSMV YNYCKDYKRD HSLTPECWR 299
Length:299
Mass (Da):34,482
Last modified:May 1, 2000 - v1
Checksum:iF14F02472ADAE61E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006922 Genomic DNA. Translation: AAD31572.1.
CP002685 Genomic DNA. Translation: AEC09308.1.
AY045840 mRNA. Translation: AAK76514.1.
AY133846 mRNA. Translation: AAM91780.1.
AY088557 mRNA. Translation: AAM66089.1.
PIRiF84785.
RefSeqiNP_181224.1. NM_129242.2. [Q9SJL9-1]
UniGeneiAt.14123.

Genome annotation databases

EnsemblPlantsiAT2G36870.1; AT2G36870.1; AT2G36870. [Q9SJL9-1]
GeneIDi818259.
KEGGiath:AT2G36870.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006922 Genomic DNA. Translation: AAD31572.1 .
CP002685 Genomic DNA. Translation: AEC09308.1 .
AY045840 mRNA. Translation: AAK76514.1 .
AY133846 mRNA. Translation: AAM91780.1 .
AY088557 mRNA. Translation: AAM66089.1 .
PIRi F84785.
RefSeqi NP_181224.1. NM_129242.2. [Q9SJL9-1 ]
UniGenei At.14123.

3D structure databases

ProteinModelPortali Q9SJL9.
SMRi Q9SJL9. Positions 27-297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G36870.1-P.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbi Q9SJL9.
PRIDEi Q9SJL9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G36870.1 ; AT2G36870.1 ; AT2G36870 . [Q9SJL9-1 ]
GeneIDi 818259.
KEGGi ath:AT2G36870.

Organism-specific databases

TAIRi AT2G36870.

Phylogenomic databases

eggNOGi NOG317325.
HOGENOMi HOG000236368.
InParanoidi Q9SJL9.
KOi K08235.
OMAi LTPECWR.
PhylomeDBi Q9SJL9.

Enzyme and pathway databases

BioCyci ARA:AT2G36870-MONOMER.
ARA:GQT-1891-MONOMER.

Gene expression databases

ArrayExpressi Q9SJL9.
Genevestigatori Q9SJL9.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
    Yokoyama R., Nishitani K.
    Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH32_ARATH
AccessioniPrimary (citable) accession number: Q9SJL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi