Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9SJL9

- XTH32_ARATH

UniProt

Q9SJL9 - XTH32_ARATH

Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 32

Gene

XTH32

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.By similarity

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei118 – 1181NucleophileBy similarity
    Active sitei122 – 1221Proton donorBy similarity

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro
    2. cell wall macromolecule catabolic process Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT2G36870-MONOMER.
    ARA:GQT-1891-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable xyloglucan endotransglucosylase/hydrolase protein 32 (EC:2.4.1.207)
    Short name:
    At-XTH32
    Short name:
    XTH-32
    Gene namesi
    Name:XTH32
    Ordered Locus Names:At2g36870
    ORF Names:T1J8.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G36870.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 299275Probable xyloglucan endotransglucosylase/hydrolase protein 32PRO_0000011832Add
    BLAST

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ9SJL9.
    PRIDEiQ9SJL9.

    Expressioni

    Tissue specificityi

    Predominantly expressed in stems.1 Publication

    Gene expression databases

    ArrayExpressiQ9SJL9.
    GenevestigatoriQ9SJL9.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G36870.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SJL9.
    SMRiQ9SJL9. Positions 27-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG317325.
    HOGENOMiHOG000236368.
    InParanoidiQ9SJL9.
    KOiK08235.
    OMAiLTPECWR.
    PhylomeDBiQ9SJL9.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9SJL9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGNSLISLLS IFHLLVLWGS SVNAYWPPSP GYWPSSKVGS LNFYKGFRNL    50
    WGPQHQRMDQ NALTIWLDRT SGSGFKSVKP FRSGYFGANI KLQPGYTAGV 100
    ITSLYLSNNE AHPGFHDEVD IEFLGTTFGK PYTLQTNVYI RGSGDGKIIG 150
    REMKFRLWFD PTKDFHHYAI LWSPREIIFL VDDIPIRRYP KKSASTFPLR 200
    PMWLYGSIWD ASSWATEDGK YKADYKYQPF TAKYTNFKAL GCTAYSSARC 250
    YPLSASPYRS GGLTRQQHQA MRWVQTHSMV YNYCKDYKRD HSLTPECWR 299
    Length:299
    Mass (Da):34,482
    Last modified:May 1, 2000 - v1
    Checksum:iF14F02472ADAE61E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006922 Genomic DNA. Translation: AAD31572.1.
    CP002685 Genomic DNA. Translation: AEC09308.1.
    AY045840 mRNA. Translation: AAK76514.1.
    AY133846 mRNA. Translation: AAM91780.1.
    AY088557 mRNA. Translation: AAM66089.1.
    PIRiF84785.
    RefSeqiNP_181224.1. NM_129242.2. [Q9SJL9-1]
    UniGeneiAt.14123.

    Genome annotation databases

    EnsemblPlantsiAT2G36870.1; AT2G36870.1; AT2G36870. [Q9SJL9-1]
    GeneIDi818259.
    KEGGiath:AT2G36870.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006922 Genomic DNA. Translation: AAD31572.1 .
    CP002685 Genomic DNA. Translation: AEC09308.1 .
    AY045840 mRNA. Translation: AAK76514.1 .
    AY133846 mRNA. Translation: AAM91780.1 .
    AY088557 mRNA. Translation: AAM66089.1 .
    PIRi F84785.
    RefSeqi NP_181224.1. NM_129242.2. [Q9SJL9-1 ]
    UniGenei At.14123.

    3D structure databases

    ProteinModelPortali Q9SJL9.
    SMRi Q9SJL9. Positions 27-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G36870.1-P.

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PaxDbi Q9SJL9.
    PRIDEi Q9SJL9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G36870.1 ; AT2G36870.1 ; AT2G36870 . [Q9SJL9-1 ]
    GeneIDi 818259.
    KEGGi ath:AT2G36870.

    Organism-specific databases

    TAIRi AT2G36870.

    Phylogenomic databases

    eggNOGi NOG317325.
    HOGENOMi HOG000236368.
    InParanoidi Q9SJL9.
    KOi K08235.
    OMAi LTPECWR.
    PhylomeDBi Q9SJL9.

    Enzyme and pathway databases

    BioCyci ARA:AT2G36870-MONOMER.
    ARA:GQT-1891-MONOMER.

    Gene expression databases

    ArrayExpressi Q9SJL9.
    Genevestigatori Q9SJL9.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
      Yokoyama R., Nishitani K.
      Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
      Rose J.K.C., Braam J., Fry S.C., Nishitani K.
      Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiXTH32_ARATH
    AccessioniPrimary (citable) accession number: Q9SJL9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3