Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 32

Gene

XTH32

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei118NucleophileBy similarity1
Active sitei122Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell wall biogenesis Source: InterPro
  • cell wall macromolecule catabolic process Source: TAIR
  • cell wall organization Source: UniProtKB-KW
  • xyloglucan metabolic process Source: InterPro

Keywordsi

Molecular functionGlycosidase, Hydrolase, Transferase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G36870-MONOMER

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 32 (EC:2.4.1.207)
Short name:
At-XTH32
Short name:
XTH-32
Gene namesi
Name:XTH32
Ordered Locus Names:At2g36870
ORF Names:T1J8.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G36870
TAIRilocus:2058006 AT2G36870

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001183225 – 299Probable xyloglucan endotransglucosylase/hydrolase protein 32Add BLAST275

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9SJL9

Expressioni

Tissue specificityi

Predominantly expressed in stems.1 Publication

Gene expression databases

ExpressionAtlasiQ9SJL9 baseline and differential
GenevisibleiQ9SJL9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G36870.1

Structurei

3D structure databases

ProteinModelPortaliQ9SJL9
SMRiQ9SJL9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 234GH16PROSITE-ProRule annotationAdd BLAST210

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJM7 Eukaryota
ENOG410YDGS LUCA
HOGENOMiHOG000236368
InParanoidiQ9SJL9
KOiK08235
OMAiYSSARCY
OrthoDBiEOG09360KA4
PhylomeDBiQ9SJL9

Family and domain databases

InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR000757 GH16
IPR010713 XET_C
IPR016455 XTH
PfamiView protein in Pfam
PF00722 Glyco_hydro_16, 1 hit
PF06955 XET_C, 1 hit
PIRSFiPIRSF005604 XET, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS51762 GH16_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SJL9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNSLISLLS IFHLLVLWGS SVNAYWPPSP GYWPSSKVGS LNFYKGFRNL
60 70 80 90 100
WGPQHQRMDQ NALTIWLDRT SGSGFKSVKP FRSGYFGANI KLQPGYTAGV
110 120 130 140 150
ITSLYLSNNE AHPGFHDEVD IEFLGTTFGK PYTLQTNVYI RGSGDGKIIG
160 170 180 190 200
REMKFRLWFD PTKDFHHYAI LWSPREIIFL VDDIPIRRYP KKSASTFPLR
210 220 230 240 250
PMWLYGSIWD ASSWATEDGK YKADYKYQPF TAKYTNFKAL GCTAYSSARC
260 270 280 290
YPLSASPYRS GGLTRQQHQA MRWVQTHSMV YNYCKDYKRD HSLTPECWR
Length:299
Mass (Da):34,482
Last modified:May 1, 2000 - v1
Checksum:iF14F02472ADAE61E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006922 Genomic DNA Translation: AAD31572.1
CP002685 Genomic DNA Translation: AEC09308.1
AY045840 mRNA Translation: AAK76514.1
AY133846 mRNA Translation: AAM91780.1
AY088557 mRNA Translation: AAM66089.1
PIRiF84785
RefSeqiNP_181224.1, NM_129242.3 [Q9SJL9-1]
UniGeneiAt.14123

Genome annotation databases

EnsemblPlantsiAT2G36870.1; AT2G36870.1; AT2G36870 [Q9SJL9-1]
GeneIDi818259
GrameneiAT2G36870.1; AT2G36870.1; AT2G36870 [Q9SJL9-1]
KEGGiath:AT2G36870

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiXTH32_ARATH
AccessioniPrimary (citable) accession number: Q9SJL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health