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Protein

S-adenosylmethionine synthase 3

Gene

METK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate (PubMed:12087191, PubMed:16365035). Involved in the biosynthesis of lignin (PubMed:11844113).3 Publications

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mn2+By similarity, Mg2+1 Publication, Co2+By similarityNote: Binds 2 divalent ions per subunit. The metal ions interact primarily with the substrate (By similarity). Can utilize magnesium, manganese or cobalt (in vitro) (By similarity).By similarity
  • K+1 PublicationNote: Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate (By similarity).By similarity

Enzyme regulationi

Inhibited by 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) and N-ethylmaleimide (NEM) (in vitro).1 Publication

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.2 Publications
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase 4 (METK4), S-adenosylmethionine synthase (At4g01850), S-adenosylmethionine synthase 2 (SAM2), S-adenosylmethionine synthase (AXX17_At2g33610), S-adenosylmethionine synthase 3 (METK3), S-adenosylmethionine synthase (AXX17_At3g18350), S-adenosylmethionine synthase (AXX17_At1g01660), S-adenosylmethionine synthase 1 (SAM1), S-adenosylmethionine synthase (AXX17_At4g02310)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumBy similarity1
Binding sitei15ATPBy similarity1
Metal bindingi43PotassiumBy similarity1
Binding sitei56MethionineBy similarity1
Binding sitei99MethionineBy similarity1
Binding sitei246ATPBy similarity1
Binding sitei246Methionine; shared with neighboring subunitBy similarity1
Binding sitei269ATP; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei273ATP; shared with neighboring subunitBy similarity1
Binding sitei277ATP; shared with neighboring subunitBy similarity1
Binding sitei277MethionineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 169ATPBy similarity3
Nucleotide bindingi235 – 238ATPBy similarity4
Nucleotide bindingi252 – 253ATPBy similarity2

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR
  • methionine adenosyltransferase activity Source: TAIR

GO - Biological processi

  • lignin biosynthetic process Source: UniProtKB-KW
  • one-carbon metabolic process Source: UniProtKB-KW
  • pollen tube growth Source: TAIR
  • S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processLignin biosynthesis, One-carbon metabolism
LigandATP-binding, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT2G36880-MONOMER
ReactomeiR-ATH-156581 Methylation
R-ATH-1614635 Sulfur amino acid metabolism
R-ATH-2408508 Metabolism of ingested SeMet, Sec, MeSec into H2Se
UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthase 3 (EC:2.5.1.61 Publication)
Short name:
AdoMet synthase 3
Alternative name(s):
Methionine adenosyltransferase 3
Short name:
MAT 3
Gene namesi
Name:METK3
Ordered Locus Names:At2g36880
ORF Names:T1J8.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G36880
TAIRilocus:2058011 AT2G36880

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120A → T in mto3-1; accumulation of free Met, increased resistance to Ethionine, and reduced lignin formation. 1 Publication1
Mutagenesisi167D → N in mto3-2; accumulation of free Met and increased resistance to Ethionine. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003630031 – 390S-adenosylmethionine synthase 3Add BLAST390

Proteomic databases

PaxDbiQ9SJL8
PRIDEiQ9SJL8

Expressioni

Tissue specificityi

Pollen (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ9SJL8 baseline and differential
GenevisibleiQ9SJL8 AT

Interactioni

Subunit structurei

Homotetramer (By similarity). Interacts with GRF3.By similarity1 Publication

Protein-protein interaction databases

BioGridi3604, 4 interactors
STRINGi3702.AT2G36880.1

Structurei

3D structure databases

ProteinModelPortaliQ9SJL8
SMRiQ9SJL8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

eggNOGiKOG1506 Eukaryota
COG0192 LUCA
HOGENOMiHOG000245710
InParanoidiQ9SJL8
KOiK00789
OMAiPWEVPKN
OrthoDBiEOG09360BUN
PhylomeDBiQ9SJL8

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SJL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METFLFTSES VNEGHPDKLC DQISDAILDA CLEQDPESKV ACETCTKTNM
60 70 80 90 100
VMVFGEITTA AKVDYEKIVR STCREIGFIS ADVGLDADKC NVLVNIEQQS
110 120 130 140 150
PDIAQGVHGH LTKKPEDIGA GDQGHMFGYA TDETPELMPL THVLATKLGA
160 170 180 190 200
KLTEVRKNKT CPWLRPDGKT QVTVEYKNDG GAMIPIRVHT VLISTQHDET
210 220 230 240 250
VTNDEIAADL KEHVIKPVIP AKYLDDNTIF HLNPSGRFVI GGPHGDAGLT
260 270 280 290 300
GRKIIIDTYG GWGAHGGGAF SGKDPTKVDR SGAYIVRQAA KSVVAAGLAR
310 320 330 340 350
RCIVQVSYAI GVPEPLSVFV DTYKTGTIPD KDILVLIKEA FDFRPGMMAI
360 370 380 390
NLDLKRGGNF RFQKTAAYGH FGRDDPDFTW EVVKPLKPKA
Length:390
Mass (Da):42,497
Last modified:May 1, 2000 - v1
Checksum:i5C8BB48838606184
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006922 Genomic DNA Translation: AAD31573.1
CP002685 Genomic DNA Translation: AEC09310.1
CP002685 Genomic DNA Translation: AEC09311.1
AF367310 mRNA Translation: AAK32897.1
AY133601 mRNA Translation: AAM91431.1
PIRiG84785
RefSeqiNP_001118458.1, NM_001124986.1
NP_181225.1, NM_129243.4
UniGeneiAt.23534

Genome annotation databases

EnsemblPlantsiAT2G36880.1; AT2G36880.1; AT2G36880
AT2G36880.2; AT2G36880.2; AT2G36880
GeneIDi818260
GrameneiAT2G36880.1; AT2G36880.1; AT2G36880
AT2G36880.2; AT2G36880.2; AT2G36880
KEGGiath:AT2G36880

Similar proteinsi

Entry informationi

Entry nameiMETK3_ARATH
AccessioniPrimary (citable) accession number: Q9SJL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health