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Protein

S-adenosylmethionine synthase 3

Gene

METK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate (PubMed:12087191, PubMed:16365035). Involved in the biosynthesis of lignin (PubMed:11844113).3 Publications

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mn2+By similarity, Mg2+1 Publication, Co2+By similarityNote: Binds 2 divalent ions per subunit. The metal ions interact primarily with the substrate (By similarity). Can utilize magnesium, manganese or cobalt (in vitro) (By similarity).By similarity
  • K+1 PublicationNote: Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate (By similarity).By similarity

Enzyme regulationi

Inhibited by 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) and N-ethylmaleimide (NEM) (in vitro).1 Publication

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.2 Publications
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase 4 (METK4), S-adenosylmethionine synthase (At4g01850), S-adenosylmethionine synthase 2 (SAM2), S-adenosylmethionine synthase (AXX17_At2g33610), S-adenosylmethionine synthase 3 (METK3), S-adenosylmethionine synthase (AXX17_At3g18350), S-adenosylmethionine synthase (AXX17_At1g01660), S-adenosylmethionine synthase 1 (SAM1), S-adenosylmethionine synthase (AXX17_At4g02310)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumBy similarity1
Binding sitei15ATPBy similarity1
Metal bindingi43PotassiumBy similarity1
Binding sitei56MethionineBy similarity1
Binding sitei99MethionineBy similarity1
Binding sitei246ATPBy similarity1
Binding sitei246Methionine; shared with neighboring subunitBy similarity1
Binding sitei269ATP; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei273ATP; shared with neighboring subunitBy similarity1
Binding sitei277ATP; shared with neighboring subunitBy similarity1
Binding sitei277MethionineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 169ATPBy similarity3
Nucleotide bindingi235 – 238ATPBy similarity4
Nucleotide bindingi252 – 253ATPBy similarity2

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR
  • methionine adenosyltransferase activity Source: TAIR

GO - Biological processi

  • lignin biosynthetic process Source: UniProtKB-KW
  • one-carbon metabolic process Source: UniProtKB-KW
  • pollen tube growth Source: TAIR
  • S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lignin biosynthesis, One-carbon metabolism

Keywords - Ligandi

ATP-binding, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT2G36880-MONOMER.
ReactomeiR-ATH-156581. Methylation.
R-ATH-1614635. Sulfur amino acid metabolism.
R-ATH-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.
UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthase 3 (EC:2.5.1.61 Publication)
Short name:
AdoMet synthase 3
Alternative name(s):
Methionine adenosyltransferase 3
Short name:
MAT 3
Gene namesi
Name:METK3
Ordered Locus Names:At2g36880
ORF Names:T1J8.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G36880.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120A → T in mto3-1; accumulation of free Met, increased resistance to Ethionine, and reduced lignin formation. 1 Publication1
Mutagenesisi167D → N in mto3-2; accumulation of free Met and increased resistance to Ethionine. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003630031 – 390S-adenosylmethionine synthase 3Add BLAST390

Proteomic databases

PaxDbiQ9SJL8.
PRIDEiQ9SJL8.

Expressioni

Tissue specificityi

Pollen (at protein level).2 Publications

Gene expression databases

GenevisibleiQ9SJL8. AT.

Interactioni

Subunit structurei

Homotetramer (By similarity). Interacts with GRF3.By similarity1 Publication

Protein-protein interaction databases

BioGridi3604. 4 interactors.
MINTiMINT-8064409.
STRINGi3702.AT2G36880.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SJL8.
SMRiQ9SJL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

eggNOGiKOG1506. Eukaryota.
COG0192. LUCA.
HOGENOMiHOG000245710.
InParanoidiQ9SJL8.
KOiK00789.
OMAiVIHINPC.
OrthoDBiEOG09360BUN.
PhylomeDBiQ9SJL8.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SJL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METFLFTSES VNEGHPDKLC DQISDAILDA CLEQDPESKV ACETCTKTNM
60 70 80 90 100
VMVFGEITTA AKVDYEKIVR STCREIGFIS ADVGLDADKC NVLVNIEQQS
110 120 130 140 150
PDIAQGVHGH LTKKPEDIGA GDQGHMFGYA TDETPELMPL THVLATKLGA
160 170 180 190 200
KLTEVRKNKT CPWLRPDGKT QVTVEYKNDG GAMIPIRVHT VLISTQHDET
210 220 230 240 250
VTNDEIAADL KEHVIKPVIP AKYLDDNTIF HLNPSGRFVI GGPHGDAGLT
260 270 280 290 300
GRKIIIDTYG GWGAHGGGAF SGKDPTKVDR SGAYIVRQAA KSVVAAGLAR
310 320 330 340 350
RCIVQVSYAI GVPEPLSVFV DTYKTGTIPD KDILVLIKEA FDFRPGMMAI
360 370 380 390
NLDLKRGGNF RFQKTAAYGH FGRDDPDFTW EVVKPLKPKA
Length:390
Mass (Da):42,497
Last modified:May 1, 2000 - v1
Checksum:i5C8BB48838606184
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006922 Genomic DNA. Translation: AAD31573.1.
CP002685 Genomic DNA. Translation: AEC09310.1.
CP002685 Genomic DNA. Translation: AEC09311.1.
AF367310 mRNA. Translation: AAK32897.1.
AY133601 mRNA. Translation: AAM91431.1.
PIRiG84785.
RefSeqiNP_001118458.1. NM_001124986.1.
NP_181225.1. NM_129243.4.
UniGeneiAt.23534.

Genome annotation databases

EnsemblPlantsiAT2G36880.1; AT2G36880.1; AT2G36880.
AT2G36880.2; AT2G36880.2; AT2G36880.
GeneIDi818260.
GrameneiAT2G36880.1; AT2G36880.1; AT2G36880.
AT2G36880.2; AT2G36880.2; AT2G36880.
KEGGiath:AT2G36880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006922 Genomic DNA. Translation: AAD31573.1.
CP002685 Genomic DNA. Translation: AEC09310.1.
CP002685 Genomic DNA. Translation: AEC09311.1.
AF367310 mRNA. Translation: AAK32897.1.
AY133601 mRNA. Translation: AAM91431.1.
PIRiG84785.
RefSeqiNP_001118458.1. NM_001124986.1.
NP_181225.1. NM_129243.4.
UniGeneiAt.23534.

3D structure databases

ProteinModelPortaliQ9SJL8.
SMRiQ9SJL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3604. 4 interactors.
MINTiMINT-8064409.
STRINGi3702.AT2G36880.1.

Proteomic databases

PaxDbiQ9SJL8.
PRIDEiQ9SJL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G36880.1; AT2G36880.1; AT2G36880.
AT2G36880.2; AT2G36880.2; AT2G36880.
GeneIDi818260.
GrameneiAT2G36880.1; AT2G36880.1; AT2G36880.
AT2G36880.2; AT2G36880.2; AT2G36880.
KEGGiath:AT2G36880.

Organism-specific databases

TAIRiAT2G36880.

Phylogenomic databases

eggNOGiKOG1506. Eukaryota.
COG0192. LUCA.
HOGENOMiHOG000245710.
InParanoidiQ9SJL8.
KOiK00789.
OMAiVIHINPC.
OrthoDBiEOG09360BUN.
PhylomeDBiQ9SJL8.

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.
BioCyciARA:AT2G36880-MONOMER.
ReactomeiR-ATH-156581. Methylation.
R-ATH-1614635. Sulfur amino acid metabolism.
R-ATH-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.

Miscellaneous databases

PROiQ9SJL8.

Gene expression databases

GenevisibleiQ9SJL8. AT.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMETK3_ARATH
AccessioniPrimary (citable) accession number: Q9SJL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.