S-adenosylmethionine synthase 3 - Arabidopsis thaliana (Mouse-ear cress)

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Q9SJL8 (METK3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 83. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
S-adenosylmethionine synthase 3
Short name=AdoMet synthase 3
EC=2.5.1.6

Alternative name(s):
Methionine adenosyltransferase 3
Short name=MAT 3

Gene names

Name:	METK3
Ordered Locus Names:	At2g36880
ORF Names:	T1J8.6

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity. Involved in the biosynthesis of lignin. Ref.4 Ref.5
Catalytic activity	ATP + L-methionine + H₂O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Enzyme regulation	Repressed by 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) and N-ethylmaleimide (NEM). Ref.8
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Pollen (at protein level). Ref.6 Ref.7
Post-translational modification	Ubiquitinated. Ref.9
Sequence similarities	Belongs to the AdoMet synthase family.

Ontologies

Keywords
Biological process	Lignin biosynthesis One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: InterPro lignin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from direct assay. Source: TAIR methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 390

390

S-adenosylmethionine synthase 3

PRO_0000363003

Regions

Nucleotide binding

119 – 124

ATP Potential

Nucleotide binding

267 – 274

ATP Potential

Sites

Metal binding

Magnesium By similarity

Metal binding

Potassium By similarity

Metal binding

271

Potassium By similarity

Metal binding

279

Magnesium By similarity

Binding site

147

ATP Potential

Experimental info

Mutagenesis

120

A → T in mto3-1; accumulation of free Met, increased resistance to Ethionine, and reduced lignin formation. Ref.5

Mutagenesis

167

D → N in mto3-2; accumulation of free Met and increased resistance to Ethionine. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q9SJL8 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 5C8BB48838606184
Show »

FASTA

390

42,497

        10         20         30         40         50         60 
METFLFTSES VNEGHPDKLC DQISDAILDA CLEQDPESKV ACETCTKTNM VMVFGEITTA 

        70         80         90        100        110        120 
AKVDYEKIVR STCREIGFIS ADVGLDADKC NVLVNIEQQS PDIAQGVHGH LTKKPEDIGA 

       130        140        150        160        170        180 
GDQGHMFGYA TDETPELMPL THVLATKLGA KLTEVRKNKT CPWLRPDGKT QVTVEYKNDG 

       190        200        210        220        230        240 
GAMIPIRVHT VLISTQHDET VTNDEIAADL KEHVIKPVIP AKYLDDNTIF HLNPSGRFVI 

       250        260        270        280        290        300 
GGPHGDAGLT GRKIIIDTYG GWGAHGGGAF SGKDPTKVDR SGAYIVRQAA KSVVAAGLAR 

       310        320        330        340        350        360 
RCIVQVSYAI GVPEPLSVFV DTYKTGTIPD KDILVLIKEA FDFRPGMMAI NLDLKRGGNF 

       370        380        390 
RFQKTAAYGH FGRDDPDFTW EVVKPLKPKA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"A single-nucleotide mutation in a gene encoding S-adenosylmethionine synthetase is associated with methionine over-accumulation phenotype in Arabidopsis thaliana." Goto D.B., Ogi M., Kijima F., Kumagai T., van Werven F., Onouchi H., Naito S. Genes Genet. Syst. 77:89-95(2002) [PubMed: 12087191] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-167.
[5]	"High free-methionine and decreased lignin content result from a mutation in the Arabidopsis S-adenosyl-L-methionine synthetase 3 gene." Shen B., Li C., Tarczynski M.C. Plant J. 29:371-380(2002) [PubMed: 11844113] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ALA-120.
[6]	"A reference map of the Arabidopsis thaliana mature pollen proteome." Noir S., Braeutigam A., Colby T., Schmidt J., Panstruga R. Biochem. Biophys. Res. Commun. 337:1257-1266(2005) [PubMed: 16242667] [Abstract] Cited for: TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
[7]	"Proteome mapping of mature pollen of Arabidopsis thaliana." Holmes-Davis R., Tanaka C.K., Vensel W.H., Hurkman W.J., McCormick S. Proteomics 5:4864-4884(2005) [PubMed: 16247729] [Abstract] Cited for: TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
[8]	"Differential inhibition of Arabidopsis methionine adenosyltransferases by protein S-nitrosylation." Lindermayr C., Saalbach G., Bahnweg G., Durner J. J. Biol. Chem. 281:4285-4291(2006) [PubMed: 16365035] [Abstract] Cited for: ENZYME REGULATION.
[9]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed: 19292762] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006922 Genomic DNA. Translation: AAD31573.1. CP002685 Genomic DNA. Translation: AEC09310.1. CP002685 Genomic DNA. Translation: AEC09311.1. AF367310 mRNA. Translation: AAK32897.1. AY133601 mRNA. Translation: AAM91431.1.
IPI	IPI00527972.
PIR	G84785.
RefSeq	NP_001118458.1. NM_001124986.1. NP_181225.1. NM_129243.3.
UniGene	At.23534.
3D structure databases
HSSP	HSSP built from PDB template 1QM4 based on UniProtKB P13444.
ProteinModelPortal	Q9SJL8.
SMR	Q9SJL8. Positions 3-387.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SJL8.
Proteomic databases
PRIDE	Q9SJL8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G36880.1; AT2G36880.1; AT2G36880. AT2G36880.2; AT2G36880.2; AT2G36880.
GeneID	818260.
GenomeReviews	Gene locus AT2G36880 in contig CT485783_GR.
KEGG	ath:AT2G36880.
NMPDR	fig\|3702.1.peg.10790.
Organism-specific databases
TAIR	At2g36880.
Phylogenomic databases
eggNOG	KOG1506.
GeneTree	EPGT00050000019410.
HOGENOM	HBG443662.
InParanoid	Q9SJL8.
OMA	RSTCREI.
PhylomeDB	Q9SJL8.
ProtClustDB	PLN02243.
Gene expression databases
ArrayExpress	Q9SJL8.
Genevestigator	Q9SJL8.
Family and domain databases
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
KO	K00789.
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. MetK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK3_ARATH

Accession

Primary (citable) accession number: Q9SJL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents