Q9SJL6 (MEM11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Membrin-11
Short name=AtMEMB11

Alternative name(s):
27 kDa Golgi SNARE protein
Golgi SNAP receptor complex member 2-1

Gene names

Name:	MEMB11
Ordered Locus Names:	At2g36900
ORF Names:	T1J8.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type IV membrane protein Potential.
Sequence similarities	Belongs to the GOSR2 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SJL6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Membrin-11		PRO_0000212554
Regions
	Topological domain	1 – 200	200	Cytoplasmic Potential
	Transmembrane	201 – 221	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	222 – 225	4	Vesicular Potential
Amino acid modifications
	Cross-link	113		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: BF92037DD3D63A22	FASTA	225	25,628
	10 20 30 40 50 60 MASGIVEGGG SLSDVYSSAK RILLKARDGI ERLERFESSS MDSPDLASSV KRDITEVRSL 70 80 90 100 110 120 CSNMDTLWRS IPVKSQRDLW RRKTEQVGEE AEYLNLSLEK YMSRNQRKML EAKERADLLG 130 140 150 160 170 180 RASGEGAHIL QIFDEEAQAM SSVKNSKRML EESFSSGVAI LSKYAEQRDR LKSAQRKALD 190 200 210 220 VLNTVGLSNS VLRLIERRNR VDTWIKYAGM IATLVILYLF IRWTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants." Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K. Mol. Cell. Proteomics 6:601-610(2007) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-113, MASS SPECTROMETRY. Strain: cv. Landsberg erecta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006922 Genomic DNA. Translation: AAD31575.1. CP002685 Genomic DNA. Translation: AEC09318.1. AY063816 mRNA. Translation: AAL36172.1. AY091350 mRNA. Translation: AAM14289.1. AY086341 mRNA. Translation: AAM64409.1.
IPI	IPI00548123.
PIR	A84786.
RefSeq	NP_181227.1. NM_129246.2.
UniGene	At.27189.
3D structure databases
ProteinModelPortal	Q9SJL6.
SMR	Q9SJL6. Positions 137-193.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SJL6. 2 interactions.
STRING	3702.AT2G36900.1-P.
Proteomic databases
PaxDb	Q9SJL6.
PRIDE	Q9SJL6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G36900.1; AT2G36900.1; AT2G36900.
GeneID	818264.
KEGG	ath:AT2G36900.
Organism-specific databases
GeneFarm	4297. 433.
TAIR	At2g36900.
Phylogenomic databases
eggNOG	NOG148687.
HOGENOM	HOG000264938.
InParanoid	Q9SJL6.
KO	K08496.
PhylomeDB	Q9SJL6.
ProtClustDB	CLSN2683951.
Gene expression databases
ArrayExpress	Q9SJL6.
Genevestigator	Q9SJL6.
GermOnline	AT2G36900. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR027027. GOSR2/Membrin/Bos1. [Graphical view]
PIRSF	PIRSF028865. Membrin-2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MEM11_ARATH

Accession

Primary (citable) accession number: Q9SJL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program