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Protein

Protein argonaute 5

Gene

AGO5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Associates with siRNAs of various sizes, from 21-24 nucleotide in length and preferentially recruits small RNAs with a 5' terminal cytosine. Probably involved in antiviral RNA silencing. Associates with siRNAs derived from cucumber mosaic virus (CMV). Targeted by the turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO5 function in RNA silencing.3 Publications

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi721Divalent metal cationBy similarity1
Metal bindingi807Divalent metal cationBy similarity1
Metal bindingi947Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

  • defense response, incompatible interaction Source: TAIR
  • gene silencing by RNA Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • regulation of translation Source: UniProtKB-KW
  • response to virus Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
  • viral gene silencing in virus induced gene silencing Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Repressor, Ribonucleoprotein

Keywords - Biological processi

Plant defense, RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-426486. Small interfering RNA (siRNA) biogenesis.
R-ATH-426496. Post-transcriptional silencing by small RNAs.
R-ATH-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute 5
Gene namesi
Name:AGO5
Ordered Locus Names:At2g27880
ORF Names:T1E2.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G27880.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • intracellular ribonucleoprotein complex Source: UniProtKB-KW
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940701 – 997Protein argonaute 5Add BLAST997

Proteomic databases

PaxDbiQ9SJK3.
PRIDEiQ9SJK3.

PTM databases

iPTMnetiQ9SJK3.

Expressioni

Gene expression databases

GenevisibleiQ9SJK3. AT.

Interactioni

Protein-protein interaction databases

BioGridi2684. 2 interactors.
MINTiMINT-8068863.
STRINGi3702.AT2G27880.1.

Structurei

Secondary structure

1997
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi563 – 567Combined sources5
Beta strandi573 – 578Combined sources6
Beta strandi581 – 583Combined sources3
Helixi587 – 602Combined sources16
Helixi619 – 621Combined sources3
Helixi622 – 632Combined sources11
Beta strandi637 – 642Combined sources6
Beta strandi645 – 648Combined sources4
Helixi650 – 658Combined sources9
Turni659 – 661Combined sources3
Beta strandi665 – 668Combined sources4
Helixi670 – 675Combined sources6
Helixi678 – 691Combined sources14
Beta strandi695 – 698Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G0OX-ray2.19A/B562-699[»]
ProteinModelPortaliQ9SJK3.
SMRiQ9SJK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini359 – 471PAZPROSITE-ProRule annotationAdd BLAST113
Domaini638 – 958PiwiPROSITE-ProRule annotationAdd BLAST321

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni847 – 848Interaction with guide RNASequence analysis2
Regioni893 – 901Interaction with guide RNASequence analysis9
Regioni930 – 952Interaction with guide RNASequence analysisAdd BLAST23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 88Gly-richAdd BLAST84

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.Curated
Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 1 Piwi domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
HOGENOMiHOG000116043.
InParanoidiQ9SJK3.
KOiK11593.
OMAiKEFGMSV.
OrthoDBiEOG093600M2.
PhylomeDBiQ9SJK3.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SJK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNRGGGGHG GASRGRGGGR RSDQRQDQSS GQVAWPGLQQ SYGGRGGSVS
60 70 80 90 100
AGRGRGNVGR GENTGDLTAT QVPVASAVSG GRGRGNIGDP TFSVASSSKT
110 120 130 140 150
VSVASSSKEE SKNTEVSETM SNLQITSTET KPEMTSLPPA SSKAVTFPVR
160 170 180 190 200
PGRGTLGKKV MVRANHFLVQ VADRDLYHYD VSINPEVISK TVNRNVMKLL
210 220 230 240 250
VKNYKDSHLG GKSPAYDGRK SLYTAGPLPF DSKEFVVNLA EKRADGSSGK
260 270 280 290 300
DRPFKVAVKN VTSTDLYQLQ QFLDRKQREA PYDTIQVLDV VLRDKPSNDY
310 320 330 340 350
VSVGRSFFHT SLGKDARDGR GELGDGIEYW RGYFQSLRLT QMGLSLNIDV
360 370 380 390 400
SARSFYEPIV VTDFISKFLN IRDLNRPLRD SDRLKVKKVL RTLKVKLLHW
410 420 430 440 450
NGTKSAKISG ISSLPIRELR FTLEDKSEKT VVQYFAEKYN YRVKYQALPA
460 470 480 490 500
IQTGSDTRPV YLPMELCQID EGQRYTKRLN EKQVTALLKA TCQRPPDREN
510 520 530 540 550
SIKNLVVKNN YNDDLSKEFG MSVTTQLASI EARVLPPPML KYHDSGKEKM
560 570 580 590 600
VNPRLGQWNM IDKKMVNGAK VTSWTCVSFS TRIDRGLPQE FCKQLIGMCV
610 620 630 640 650
SKGMEFKPQP AIPFISCPPE HIEEALLDIH KRAPGLQLLI VILPDVTGSY
660 670 680 690 700
GKIKRICETE LGIVSQCCQP RQVNKLNKQY MENVALKINV KTGGRNTVLN
710 720 730 740 750
DAIRRNIPLI TDRPTIIMGA DVTHPQPGED SSPSIAAVVA SMDWPEINKY
760 770 780 790 800
RGLVSAQAHR EEIIQDLYKL VQDPQRGLVH SGLIREHFIA FRRATGQIPQ
810 820 830 840 850
RIIFYRDGVS EGQFSQVLLH EMTAIRKACN SLQENYVPRV TFVIVQKRHH
860 870 880 890 900
TRLFPEQHGN RDMTDKSGNI QPGTVVDTKI CHPNEFDFYL NSHAGIQGTS
910 920 930 940 950
RPAHYHVLLD ENGFTADQLQ MLTNNLCYTY ARCTKSVSIV PPAYYAHLAA
960 970 980 990
FRARYYMESE MSDGGSSRSR SSTTGVGQVI SQLPAIKDNV KEVMFYC
Length:997
Mass (Da):111,089
Last modified:February 8, 2011 - v2
Checksum:iBEA510811ECC3666
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti402G → C in AAD21514 (PubMed:10617197).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006929 Genomic DNA. Translation: AAD21514.1.
CP002685 Genomic DNA. Translation: AEC08055.1.
PIRiA84678.
RefSeqiNP_850110.1. NM_179779.3.
UniGeneiAt.50103.

Genome annotation databases

EnsemblPlantsiAT2G27880.1; AT2G27880.1; AT2G27880.
GeneIDi817334.
GrameneiAT2G27880.1; AT2G27880.1; AT2G27880.
KEGGiath:AT2G27880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006929 Genomic DNA. Translation: AAD21514.1.
CP002685 Genomic DNA. Translation: AEC08055.1.
PIRiA84678.
RefSeqiNP_850110.1. NM_179779.3.
UniGeneiAt.50103.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G0OX-ray2.19A/B562-699[»]
ProteinModelPortaliQ9SJK3.
SMRiQ9SJK3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2684. 2 interactors.
MINTiMINT-8068863.
STRINGi3702.AT2G27880.1.

PTM databases

iPTMnetiQ9SJK3.

Proteomic databases

PaxDbiQ9SJK3.
PRIDEiQ9SJK3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G27880.1; AT2G27880.1; AT2G27880.
GeneIDi817334.
GrameneiAT2G27880.1; AT2G27880.1; AT2G27880.
KEGGiath:AT2G27880.

Organism-specific databases

TAIRiAT2G27880.

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
HOGENOMiHOG000116043.
InParanoidiQ9SJK3.
KOiK11593.
OMAiKEFGMSV.
OrthoDBiEOG093600M2.
PhylomeDBiQ9SJK3.

Enzyme and pathway databases

ReactomeiR-ATH-426486. Small interfering RNA (siRNA) biogenesis.
R-ATH-426496. Post-transcriptional silencing by small RNAs.
R-ATH-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

PROiQ9SJK3.

Gene expression databases

GenevisibleiQ9SJK3. AT.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGO5_ARATH
AccessioniPrimary (citable) accession number: Q9SJK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 8, 2011
Last modified: November 30, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.