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Protein

Multiprotein-bridging factor 1a

Gene

MBF1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional coactivator that stimulates transcriptional activity by bridging regulatory proteins and TBP, thereby recruiting TBP to promoters occupied by DNA-binding regulators.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi98 – 117H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • sequence-specific DNA binding Source: InterPro
  • transcription coactivator activity Source: TAIR

GO - Biological processi

  • positive regulation of transcription, DNA-templated Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Multiprotein-bridging factor 1a
Gene namesi
Name:MBF1A
Ordered Locus Names:At2g42680
ORF Names:F14N22.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G42680.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003259031 – 142Multiprotein-bridging factor 1aAdd BLAST142

Proteomic databases

PaxDbiQ9SJI8.
PRIDEiQ9SJI8.

PTM databases

iPTMnetiQ9SJI8.

Expressioni

Tissue specificityi

Expressed in leaves, roots, stems, flowers, siliques and shoots. Detected only in anthers and some seeds in siliques.2 Publications

Developmental stagei

Detected in some seeds from 3 days after pollination (dap) to 11 dap.1 Publication

Inductioni

Not induced by heat or cold treatments, H2O2, dehydration, abscisic acid, 2,4-D, ACC, methyl jasmonate or salicylic acid. Weak induction by salt stress and pathogen induction.4 Publications

Gene expression databases

GenevisibleiQ9SJI8. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G42680.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SJI8.
SMRiQ9SJI8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 141HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST55

Domaini

The C-terminal domain (75-142) is essential for nucleolar localization.

Sequence similaritiesi

Belongs to the MBF1 family.Curated
Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3398. Eukaryota.
COG1813. LUCA.
HOGENOMiHOG000195726.
InParanoidiQ9SJI8.
KOiK03627.
OMAiYECGRAI.
OrthoDBiEOG09360RDR.
PhylomeDBiQ9SJI8.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR013729. MBF1_N.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF08523. MBF1. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SJI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIGPITQD WEPVVIRKKP ANAAAKRDEK TVNAARRSGA DIETVRKFNA
60 70 80 90 100
GTNKAASSGT SLNTKMLDDD TENLTHERVP TELKKAIMQA RTDKKLTQSQ
110 120 130 140
LAQIINEKPQ VIQEYESGKA IPNQQILSKL ERALGAKLRG KK
Length:142
Mass (Da):15,620
Last modified:May 1, 2000 - v1
Checksum:i76A789084B41E97B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006931 Genomic DNA. Translation: AAD21738.1.
AC007087 Genomic DNA. Translation: AAM15391.1.
CP002685 Genomic DNA. Translation: AEC10155.1.
AF370280 mRNA. Translation: AAK44095.1.
AY063014 mRNA. Translation: AAL34188.1.
AY088140 mRNA. Translation: AAM65685.1.
PIRiH84856.
RefSeqiNP_565981.1. NM_129829.4.
UniGeneiAt.14148.
At.24758.

Genome annotation databases

EnsemblPlantsiAT2G42680.1; AT2G42680.1; AT2G42680.
GeneIDi818868.
GrameneiAT2G42680.1; AT2G42680.1; AT2G42680.
KEGGiath:AT2G42680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006931 Genomic DNA. Translation: AAD21738.1.
AC007087 Genomic DNA. Translation: AAM15391.1.
CP002685 Genomic DNA. Translation: AEC10155.1.
AF370280 mRNA. Translation: AAK44095.1.
AY063014 mRNA. Translation: AAL34188.1.
AY088140 mRNA. Translation: AAM65685.1.
PIRiH84856.
RefSeqiNP_565981.1. NM_129829.4.
UniGeneiAt.14148.
At.24758.

3D structure databases

ProteinModelPortaliQ9SJI8.
SMRiQ9SJI8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G42680.1.

PTM databases

iPTMnetiQ9SJI8.

Proteomic databases

PaxDbiQ9SJI8.
PRIDEiQ9SJI8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G42680.1; AT2G42680.1; AT2G42680.
GeneIDi818868.
GrameneiAT2G42680.1; AT2G42680.1; AT2G42680.
KEGGiath:AT2G42680.

Organism-specific databases

TAIRiAT2G42680.

Phylogenomic databases

eggNOGiKOG3398. Eukaryota.
COG1813. LUCA.
HOGENOMiHOG000195726.
InParanoidiQ9SJI8.
KOiK03627.
OMAiYECGRAI.
OrthoDBiEOG09360RDR.
PhylomeDBiQ9SJI8.

Miscellaneous databases

PROiQ9SJI8.

Gene expression databases

GenevisibleiQ9SJI8. AT.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR013729. MBF1_N.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF08523. MBF1. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBF1A_ARATH
AccessioniPrimary (citable) accession number: Q9SJI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.