ID   CISY3_ARATH             Reviewed;         509 AA.
AC   Q9SJH7; Q56YQ7;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   27-MAR-2024, entry version 143.
DE   RecName: Full=Citrate synthase 3, peroxisomal;
DE            EC=2.3.3.16;
DE   Flags: Precursor;
GN   Name=CSY3; OrderedLocusNames=At2g42790; ORFNames=F7D19.21;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 369-509.
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=15923350; DOI=10.1105/tpc.105.031856;
RA   Pracharoenwattana I., Cornah J.E., Smith S.M.;
RT   "Arabidopsis peroxisomal citrate synthase is required for fatty acid
RT   respiration and seed germination.";
RL   Plant Cell 17:2037-2048(2005).
CC   -!- FUNCTION: Peroxisomal citrate synthase required for the fatty acid
CC       respiration in seedlings, citrate being exported from peroxisomes into
CC       mitochondria during respiration of triacylglycerol (TAG). Indeed,
CC       complete respiration requires the transfer of carbon in the form of
CC       citrate from the peroxisome to the mitochondria.
CC       {ECO:0000269|PubMed:15923350}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:15923350}.
CC   -!- TISSUE SPECIFICITY: Widely expressed. Expressed throughout the shoot.
CC       Expressed in flower, silique, stem, cauline leaf, young leaf, mature
CC       leaf and senescent leaf. {ECO:0000269|PubMed:15923350}.
CC   -!- DEVELOPMENTAL STAGE: Expressed throughout seedling growth.
CC       {ECO:0000269|PubMed:15923350}.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; AC006931; AAD21729.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC10169.1; -; Genomic_DNA.
DR   EMBL; AY048214; AAK82477.1; -; mRNA.
DR   EMBL; AY124841; AAM70550.1; -; mRNA.
DR   EMBL; AK221264; BAD93930.1; -; mRNA.
DR   PIR; C84858; C84858.
DR   RefSeq; NP_181807.1; NM_129840.5.
DR   AlphaFoldDB; Q9SJH7; -.
DR   SMR; Q9SJH7; -.
DR   BioGRID; 4216; 33.
DR   IntAct; Q9SJH7; 17.
DR   STRING; 3702.Q9SJH7; -.
DR   PaxDb; 3702-AT2G42790-1; -.
DR   ProteomicsDB; 246864; -.
DR   EnsemblPlants; AT2G42790.1; AT2G42790.1; AT2G42790.
DR   GeneID; 818879; -.
DR   Gramene; AT2G42790.1; AT2G42790.1; AT2G42790.
DR   KEGG; ath:AT2G42790; -.
DR   Araport; AT2G42790; -.
DR   TAIR; AT2G42790; CSY3.
DR   eggNOG; KOG2617; Eukaryota.
DR   HOGENOM; CLU_025068_0_1_1; -.
DR   InParanoid; Q9SJH7; -.
DR   OMA; QGFMAHW; -.
DR   OrthoDB; 642531at2759; -.
DR   PhylomeDB; Q9SJH7; -.
DR   BioCyc; ARA:AT2G42790-MONOMER; -.
DR   UniPathway; UPA00223; UER00717.
DR   PRO; PR:Q9SJH7; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q9SJH7; baseline and differential.
DR   GO; GO:0005829; C:cytosol; HDA:TAIR.
DR   GO; GO:0005777; C:peroxisome; IDA:TAIR.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IGI:TAIR.
DR   GO; GO:0006635; P:fatty acid beta-oxidation; IGI:TAIR.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06115; AthCS_per_like; 1.
DR   Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR   Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR   PANTHER; PTHR11739:SF4; CITRATE SYNTHASE, PEROXISOMAL; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; Citrate synthase; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
DR   Genevisible; Q9SJH7; AT.
PE   2: Evidence at transcript level;
KW   Peroxisome; Reference proteome; Transferase; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT         1..?
FT                   /note="Peroxisome"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..509
FT                   /note="Citrate synthase 3, peroxisomal"
FT                   /id="PRO_0000005483"
FT   REGION          485..509
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        491..509
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        319
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        358
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        414
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   CONFLICT        430
FT                   /note="F -> L (in Ref. 4; BAD93930)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   509 AA;  56175 MW;  5ADF4A63F1DDFE38 CRC64;
     MEISERVRAR LAVLSGHLSE GKQDSPAIER WCTSADTSVA PLGSLKGTLT IVDERTGKNY
     KVPVSDDGTV KAVDFKKIVT GKEDKGLKLY DPGYLNTAPV RSSISYIDGD EGILRYRGYP
     IEEMAENSTF LEVAYLLMYG NLPSESQLSD WEFAVSQHSA VPQGVLDIIQ SMPHDAHPMG
     VLVSAMSALS IFHPDANPAL RGQDIYDSKQ VRDKQIIRII GKAPTIAAAA YLRMAGRPPV
     LPSGNLPYAD NFLYMLDSLG NRSYKPNPRL ARVLDILFIL HAEHEMNCST AAARHLASSG
     VDVYTAVAGA VGALYGPLHG GANEAVLKML SEIGTVENIP EFIEGVKNRK RKMSGFGHRV
     YKNYDPRAKV IKNLADEVFS IVGKDPLIEV AVALEKAALS DDYFVKRKLY PNVDFYSGLI
     YRAMGFPPEF FTVLFAIPRM AGYLSHWKES LDDPDTKIMR PQQVYTGVWL RHYTPVRERI
     VTDDSKESDK LGQVATSNAS RRRLAGSSV
//