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Q9SJH7

- CISY3_ARATH

UniProt

Q9SJH7 - CISY3_ARATH

Protein

Citrate synthase 3, peroxisomal

Gene

CSY3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Peroxisomal citrate synthase required for the fatty acid respiration in seedlings, citrate being exported from peroxisomes into mitochondria during respiration of triacylglycerol (TAG). Indeed, complete respiration requires the transfer of carbon in the form of citrate from the peroxisome to the mitochondria.1 Publication

    Catalytic activityi

    Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei319 – 3191PROSITE-ProRule annotation
    Active sitei358 – 3581PROSITE-ProRule annotation
    Active sitei414 – 4141PROSITE-ProRule annotation

    GO - Molecular functioni

    1. citrate (Si)-synthase activity Source: TAIR

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. fatty acid beta-oxidation Source: TAIR
    3. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Enzyme and pathway databases

    BioCyciARA:AT2G42790-MONOMER.
    UniPathwayiUPA00223; UER00717.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Citrate synthase 3, peroxisomal (EC:2.3.3.16)
    Gene namesi
    Name:CSY3
    Ordered Locus Names:At2g42790
    ORF Names:F7D19.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G42790.

    Subcellular locationi

    Peroxisome 1 Publication

    GO - Cellular componenti

    1. peroxisome Source: TAIR

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 509Citrate synthase 3, peroxisomalPRO_0000005483
    Transit peptidei1 – ?PeroxisomeSequence Analysis

    Proteomic databases

    PaxDbiQ9SJH7.
    PRIDEiQ9SJH7.

    Expressioni

    Tissue specificityi

    Widely expressed. Expressed throughout the shoot. Expressed in flower, silique, stem, cauline leaf, young leaf, mature leaf and senescent leaf.1 Publication

    Developmental stagei

    Expressed throughout seedling growth.1 Publication

    Gene expression databases

    ArrayExpressiQ9SJH7.
    GenevestigatoriQ9SJH7.

    Interactioni

    Protein-protein interaction databases

    BioGridi4216. 13 interactions.
    IntActiQ9SJH7. 17 interactions.
    STRINGi3702.AT2G42790.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SJH7.
    SMRiQ9SJH7. Positions 57-479.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the citrate synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0372.
    HOGENOMiHOG000021224.
    InParanoidiQ9SJH7.
    KOiK01647.
    PhylomeDBiQ9SJH7.

    Family and domain databases

    Gene3Di1.10.230.10. 1 hit.
    1.10.580.10. 1 hit.
    InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
    IPR016143. Citrate_synth-like_sm_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR024176. Citrate_synthase_bac-typ.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001369. Citrate_synth. 1 hit.
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SJH7-1 [UniParc]FASTAAdd to Basket

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    MEISERVRAR LAVLSGHLSE GKQDSPAIER WCTSADTSVA PLGSLKGTLT    50
    IVDERTGKNY KVPVSDDGTV KAVDFKKIVT GKEDKGLKLY DPGYLNTAPV 100
    RSSISYIDGD EGILRYRGYP IEEMAENSTF LEVAYLLMYG NLPSESQLSD 150
    WEFAVSQHSA VPQGVLDIIQ SMPHDAHPMG VLVSAMSALS IFHPDANPAL 200
    RGQDIYDSKQ VRDKQIIRII GKAPTIAAAA YLRMAGRPPV LPSGNLPYAD 250
    NFLYMLDSLG NRSYKPNPRL ARVLDILFIL HAEHEMNCST AAARHLASSG 300
    VDVYTAVAGA VGALYGPLHG GANEAVLKML SEIGTVENIP EFIEGVKNRK 350
    RKMSGFGHRV YKNYDPRAKV IKNLADEVFS IVGKDPLIEV AVALEKAALS 400
    DDYFVKRKLY PNVDFYSGLI YRAMGFPPEF FTVLFAIPRM AGYLSHWKES 450
    LDDPDTKIMR PQQVYTGVWL RHYTPVRERI VTDDSKESDK LGQVATSNAS 500
    RRRLAGSSV 509
    Length:509
    Mass (Da):56,175
    Last modified:May 1, 2000 - v1
    Checksum:i5ADF4A63F1DDFE38
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti430 – 4301F → L in BAD93930. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006931 Genomic DNA. Translation: AAD21729.1.
    CP002685 Genomic DNA. Translation: AEC10169.1.
    AY048214 mRNA. Translation: AAK82477.1.
    AY124841 mRNA. Translation: AAM70550.1.
    AK221264 mRNA. Translation: BAD93930.1.
    PIRiC84858.
    RefSeqiNP_181807.1. NM_129840.4.
    UniGeneiAt.19848.
    At.75577.

    Genome annotation databases

    EnsemblPlantsiAT2G42790.1; AT2G42790.1; AT2G42790.
    GeneIDi818879.
    KEGGiath:AT2G42790.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006931 Genomic DNA. Translation: AAD21729.1 .
    CP002685 Genomic DNA. Translation: AEC10169.1 .
    AY048214 mRNA. Translation: AAK82477.1 .
    AY124841 mRNA. Translation: AAM70550.1 .
    AK221264 mRNA. Translation: BAD93930.1 .
    PIRi C84858.
    RefSeqi NP_181807.1. NM_129840.4.
    UniGenei At.19848.
    At.75577.

    3D structure databases

    ProteinModelPortali Q9SJH7.
    SMRi Q9SJH7. Positions 57-479.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4216. 13 interactions.
    IntActi Q9SJH7. 17 interactions.
    STRINGi 3702.AT2G42790.1-P.

    Proteomic databases

    PaxDbi Q9SJH7.
    PRIDEi Q9SJH7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G42790.1 ; AT2G42790.1 ; AT2G42790 .
    GeneIDi 818879.
    KEGGi ath:AT2G42790.

    Organism-specific databases

    TAIRi AT2G42790.

    Phylogenomic databases

    eggNOGi COG0372.
    HOGENOMi HOG000021224.
    InParanoidi Q9SJH7.
    KOi K01647.
    PhylomeDBi Q9SJH7.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER00717 .
    BioCyci ARA:AT2G42790-MONOMER.

    Gene expression databases

    ArrayExpressi Q9SJH7.
    Genevestigatori Q9SJH7.

    Family and domain databases

    Gene3Di 1.10.230.10. 1 hit.
    1.10.580.10. 1 hit.
    InterProi IPR016142. Citrate_synth-like_lrg_a-sub.
    IPR016143. Citrate_synth-like_sm_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR024176. Citrate_synthase_bac-typ.
    [Graphical view ]
    PANTHERi PTHR11739. PTHR11739. 1 hit.
    Pfami PF00285. Citrate_synt. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001369. Citrate_synth. 1 hit.
    PRINTSi PR00143. CITRTSNTHASE.
    SUPFAMi SSF48256. SSF48256. 1 hit.
    PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 369-509.
      Strain: cv. Columbia.
    5. "Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination."
      Pracharoenwattana I., Cornah J.E., Smith S.M.
      Plant Cell 17:2037-2048(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiCISY3_ARATH
    AccessioniPrimary (citable) accession number: Q9SJH7
    Secondary accession number(s): Q56YQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Citrate synthase is found in nearly all cells capable of oxidative metabolism.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3