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Protein

Citrate synthase 3, peroxisomal

Gene

CSY3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Peroxisomal citrate synthase required for the fatty acid respiration in seedlings, citrate being exported from peroxisomes into mitochondria during respiration of triacylglycerol (TAG). Indeed, complete respiration requires the transfer of carbon in the form of citrate from the peroxisome to the mitochondria.1 Publication

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei319 – 3191PROSITE-ProRule annotation
Active sitei358 – 3581PROSITE-ProRule annotation
Active sitei414 – 4141PROSITE-ProRule annotation

GO - Molecular functioni

  1. citrate (Si)-synthase activity Source: TAIR

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. fatty acid beta-oxidation Source: TAIR
  3. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciARA:AT2G42790-MONOMER.
ReactomeiREACT_242439. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase 3, peroxisomal (EC:2.3.3.16)
Gene namesi
Name:CSY3
Ordered Locus Names:At2g42790
ORF Names:F7D19.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G42790.

Subcellular locationi

Peroxisome 1 Publication

GO - Cellular componenti

  1. peroxisome Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 509Citrate synthase 3, peroxisomalPRO_0000005483
Transit peptidei1 – ?PeroxisomeSequence Analysis

Proteomic databases

PaxDbiQ9SJH7.
PRIDEiQ9SJH7.

Expressioni

Tissue specificityi

Widely expressed. Expressed throughout the shoot. Expressed in flower, silique, stem, cauline leaf, young leaf, mature leaf and senescent leaf.1 Publication

Developmental stagei

Expressed throughout seedling growth.1 Publication

Gene expression databases

ExpressionAtlasiQ9SJH7. baseline and differential.
GenevestigatoriQ9SJH7.

Interactioni

Protein-protein interaction databases

BioGridi4216. 13 interactions.
IntActiQ9SJH7. 17 interactions.
STRINGi3702.AT2G42790.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SJH7.
SMRiQ9SJH7. Positions 57-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
InParanoidiQ9SJH7.
KOiK01647.
OMAiVERIPEF.
PhylomeDBiQ9SJH7.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SJH7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEISERVRAR LAVLSGHLSE GKQDSPAIER WCTSADTSVA PLGSLKGTLT
60 70 80 90 100
IVDERTGKNY KVPVSDDGTV KAVDFKKIVT GKEDKGLKLY DPGYLNTAPV
110 120 130 140 150
RSSISYIDGD EGILRYRGYP IEEMAENSTF LEVAYLLMYG NLPSESQLSD
160 170 180 190 200
WEFAVSQHSA VPQGVLDIIQ SMPHDAHPMG VLVSAMSALS IFHPDANPAL
210 220 230 240 250
RGQDIYDSKQ VRDKQIIRII GKAPTIAAAA YLRMAGRPPV LPSGNLPYAD
260 270 280 290 300
NFLYMLDSLG NRSYKPNPRL ARVLDILFIL HAEHEMNCST AAARHLASSG
310 320 330 340 350
VDVYTAVAGA VGALYGPLHG GANEAVLKML SEIGTVENIP EFIEGVKNRK
360 370 380 390 400
RKMSGFGHRV YKNYDPRAKV IKNLADEVFS IVGKDPLIEV AVALEKAALS
410 420 430 440 450
DDYFVKRKLY PNVDFYSGLI YRAMGFPPEF FTVLFAIPRM AGYLSHWKES
460 470 480 490 500
LDDPDTKIMR PQQVYTGVWL RHYTPVRERI VTDDSKESDK LGQVATSNAS

RRRLAGSSV
Length:509
Mass (Da):56,175
Last modified:May 1, 2000 - v1
Checksum:i5ADF4A63F1DDFE38
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti430 – 4301F → L in BAD93930. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006931 Genomic DNA. Translation: AAD21729.1.
CP002685 Genomic DNA. Translation: AEC10169.1.
AY048214 mRNA. Translation: AAK82477.1.
AY124841 mRNA. Translation: AAM70550.1.
AK221264 mRNA. Translation: BAD93930.1.
PIRiC84858.
RefSeqiNP_181807.1. NM_129840.4.
UniGeneiAt.19848.
At.75577.

Genome annotation databases

EnsemblPlantsiAT2G42790.1; AT2G42790.1; AT2G42790.
GeneIDi818879.
KEGGiath:AT2G42790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006931 Genomic DNA. Translation: AAD21729.1.
CP002685 Genomic DNA. Translation: AEC10169.1.
AY048214 mRNA. Translation: AAK82477.1.
AY124841 mRNA. Translation: AAM70550.1.
AK221264 mRNA. Translation: BAD93930.1.
PIRiC84858.
RefSeqiNP_181807.1. NM_129840.4.
UniGeneiAt.19848.
At.75577.

3D structure databases

ProteinModelPortaliQ9SJH7.
SMRiQ9SJH7. Positions 57-479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4216. 13 interactions.
IntActiQ9SJH7. 17 interactions.
STRINGi3702.AT2G42790.1-P.

Proteomic databases

PaxDbiQ9SJH7.
PRIDEiQ9SJH7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G42790.1; AT2G42790.1; AT2G42790.
GeneIDi818879.
KEGGiath:AT2G42790.

Organism-specific databases

TAIRiAT2G42790.

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
InParanoidiQ9SJH7.
KOiK01647.
OMAiVERIPEF.
PhylomeDBiQ9SJH7.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.
BioCyciARA:AT2G42790-MONOMER.
ReactomeiREACT_242439. Citric acid cycle (TCA cycle).

Gene expression databases

ExpressionAtlasiQ9SJH7. baseline and differential.
GenevestigatoriQ9SJH7.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 369-509.
    Strain: cv. Columbia.
  5. "Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination."
    Pracharoenwattana I., Cornah J.E., Smith S.M.
    Plant Cell 17:2037-2048(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCISY3_ARATH
AccessioniPrimary (citable) accession number: Q9SJH7
Secondary accession number(s): Q56YQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.