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Protein

Citrate synthase 3, peroxisomal

Gene

CSY3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Peroxisomal citrate synthase required for the fatty acid respiration in seedlings, citrate being exported from peroxisomes into mitochondria during respiration of triacylglycerol (TAG). Indeed, complete respiration requires the transfer of carbon in the form of citrate from the peroxisome to the mitochondria.1 Publication

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase 5, mitochondrial (CSY5), Citrate synthase 1, peroxisomal (CSY1), Citrate synthase 2, peroxisomal (CSY2), Citrate synthase 3, peroxisomal (CSY3), Citrate synthase 4, mitochondrial (CSY4)
  2. Aconitate hydratase 3, mitochondrial (ACO3), Aconitate hydratase 1 (ACO1), Aconitate hydratase 2, mitochondrial (ACO2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei319PROSITE-ProRule annotation1
Active sitei358PROSITE-ProRule annotation1
Active sitei414PROSITE-ProRule annotation1

GO - Molecular functioni

  • citrate (Si)-synthase activity Source: TAIR

GO - Biological processi

  • fatty acid beta-oxidation Source: TAIR
  • tricarboxylic acid cycle Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

BioCyciARA:AT2G42790-MONOMER
UniPathwayiUPA00223; UER00717

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase 3, peroxisomal (EC:2.3.3.16)
Gene namesi
Name:CSY3
Ordered Locus Names:At2g42790
ORF Names:F7D19.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G42790
TAIRilocus:2052379 AT2G42790

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000005483? – 509Citrate synthase 3, peroxisomal
Transit peptidei1 – ?PeroxisomeSequence analysis

Proteomic databases

PaxDbiQ9SJH7
PRIDEiQ9SJH7

Expressioni

Tissue specificityi

Widely expressed. Expressed throughout the shoot. Expressed in flower, silique, stem, cauline leaf, young leaf, mature leaf and senescent leaf.1 Publication

Developmental stagei

Expressed throughout seedling growth.1 Publication

Gene expression databases

ExpressionAtlasiQ9SJH7 baseline and differential
GenevisibleiQ9SJH7 AT

Interactioni

Protein-protein interaction databases

BioGridi4216, 13 interactors
IntActiQ9SJH7, 17 interactors
STRINGi3702.AT2G42790.1

Structurei

3D structure databases

ProteinModelPortaliQ9SJH7
SMRiQ9SJH7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2617 Eukaryota
COG0372 LUCA
HOGENOMiHOG000021224
InParanoidiQ9SJH7
KOiK01647
OMAiFLYQMDH
OrthoDBiEOG0936084F
PhylomeDBiQ9SJH7

Family and domain databases

Gene3Di1.10.230.10, 1 hit
1.10.580.10, 2 hits
InterProiView protein in InterPro
IPR016142 Citrate_synth-like_lrg_a-sub
IPR016143 Citrate_synth-like_sm_a-sub
IPR002020 Citrate_synthase
IPR019810 Citrate_synthase_AS
IPR024176 Citrate_synthase_bac-typ
IPR036969 Citrate_synthase_sf
PANTHERiPTHR11739 PTHR11739, 1 hit
PfamiView protein in Pfam
PF00285 Citrate_synt, 1 hit
PIRSFiPIRSF001369 Citrate_synth, 1 hit
PRINTSiPR00143 CITRTSNTHASE
SUPFAMiSSF48256 SSF48256, 1 hit
PROSITEiView protein in PROSITE
PS00480 CITRATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SJH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEISERVRAR LAVLSGHLSE GKQDSPAIER WCTSADTSVA PLGSLKGTLT
60 70 80 90 100
IVDERTGKNY KVPVSDDGTV KAVDFKKIVT GKEDKGLKLY DPGYLNTAPV
110 120 130 140 150
RSSISYIDGD EGILRYRGYP IEEMAENSTF LEVAYLLMYG NLPSESQLSD
160 170 180 190 200
WEFAVSQHSA VPQGVLDIIQ SMPHDAHPMG VLVSAMSALS IFHPDANPAL
210 220 230 240 250
RGQDIYDSKQ VRDKQIIRII GKAPTIAAAA YLRMAGRPPV LPSGNLPYAD
260 270 280 290 300
NFLYMLDSLG NRSYKPNPRL ARVLDILFIL HAEHEMNCST AAARHLASSG
310 320 330 340 350
VDVYTAVAGA VGALYGPLHG GANEAVLKML SEIGTVENIP EFIEGVKNRK
360 370 380 390 400
RKMSGFGHRV YKNYDPRAKV IKNLADEVFS IVGKDPLIEV AVALEKAALS
410 420 430 440 450
DDYFVKRKLY PNVDFYSGLI YRAMGFPPEF FTVLFAIPRM AGYLSHWKES
460 470 480 490 500
LDDPDTKIMR PQQVYTGVWL RHYTPVRERI VTDDSKESDK LGQVATSNAS

RRRLAGSSV
Length:509
Mass (Da):56,175
Last modified:May 1, 2000 - v1
Checksum:i5ADF4A63F1DDFE38
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti430F → L in BAD93930 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006931 Genomic DNA Translation: AAD21729.1
CP002685 Genomic DNA Translation: AEC10169.1
AY048214 mRNA Translation: AAK82477.1
AY124841 mRNA Translation: AAM70550.1
AK221264 mRNA Translation: BAD93930.1
PIRiC84858
RefSeqiNP_181807.1, NM_129840.5
UniGeneiAt.19848
At.75577

Genome annotation databases

EnsemblPlantsiAT2G42790.1; AT2G42790.1; AT2G42790
GeneIDi818879
GrameneiAT2G42790.1; AT2G42790.1; AT2G42790
KEGGiath:AT2G42790

Similar proteinsi

Entry informationi

Entry nameiCISY3_ARATH
AccessioniPrimary (citable) accession number: Q9SJH7
Secondary accession number(s): Q56YQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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