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Q9SJH7 (CISY3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase 3, peroxisomal

EC=2.3.3.1
Gene names
Name:CSY3
Ordered Locus Names:At2g42790
ORF Names:F7D19.21
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Peroxisomal citrate synthase required for the fatty acid respiration in seedlings, citrate being exported from peroxisomes into mitochondria during respiration of triacylglycerol (TAG). Indeed, complete respiration requires the transfer of carbon in the form of citrate from the peroxisome to the mitochondria. Ref.5

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subcellular location

Peroxisome Ref.5.

Tissue specificity

Widely expressed. Expressed throughout the shoot. Expressed in flower, silique, stem, cauline leaf, young leaf, mature leaf and senescent leaf. Ref.5

Developmental stage

Expressed throughout seedling growth. Ref.5

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentPeroxisome
   DomainTransit peptide
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

fatty acid beta-oxidation

Inferred from genetic interaction Ref.5. Source: TAIR

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentperoxisome

Inferred from direct assay Ref.5PubMed 17951448. Source: TAIR

   Molecular_functioncitrate (Si)-synthase activity

Inferred from genetic interaction Ref.5. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Peroxisome Potential
Chain? – 509Citrate synthase 3, peroxisomalPRO_0000005483

Sites

Active site3191 By similarity
Active site3581 By similarity
Active site4141 By similarity

Experimental info

Sequence conflict4301F → L in BAD93930. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9SJH7 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 5ADF4A63F1DDFE38

FASTA50956,175
        10         20         30         40         50         60 
MEISERVRAR LAVLSGHLSE GKQDSPAIER WCTSADTSVA PLGSLKGTLT IVDERTGKNY 

        70         80         90        100        110        120 
KVPVSDDGTV KAVDFKKIVT GKEDKGLKLY DPGYLNTAPV RSSISYIDGD EGILRYRGYP 

       130        140        150        160        170        180 
IEEMAENSTF LEVAYLLMYG NLPSESQLSD WEFAVSQHSA VPQGVLDIIQ SMPHDAHPMG 

       190        200        210        220        230        240 
VLVSAMSALS IFHPDANPAL RGQDIYDSKQ VRDKQIIRII GKAPTIAAAA YLRMAGRPPV 

       250        260        270        280        290        300 
LPSGNLPYAD NFLYMLDSLG NRSYKPNPRL ARVLDILFIL HAEHEMNCST AAARHLASSG 

       310        320        330        340        350        360 
VDVYTAVAGA VGALYGPLHG GANEAVLKML SEIGTVENIP EFIEGVKNRK RKMSGFGHRV 

       370        380        390        400        410        420 
YKNYDPRAKV IKNLADEVFS IVGKDPLIEV AVALEKAALS DDYFVKRKLY PNVDFYSGLI 

       430        440        450        460        470        480 
YRAMGFPPEF FTVLFAIPRM AGYLSHWKES LDDPDTKIMR PQQVYTGVWL RHYTPVRERI 

       490        500 
VTDDSKESDK LGQVATSNAS RRRLAGSSV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 369-509.
Strain: cv. Columbia.
[5]"Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination."
Pracharoenwattana I., Cornah J.E., Smith S.M.
Plant Cell 17:2037-2048(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006931 Genomic DNA. Translation: AAD21729.1.
CP002685 Genomic DNA. Translation: AEC10169.1.
AY048214 mRNA. Translation: AAK82477.1.
AY124841 mRNA. Translation: AAM70550.1.
AK221264 mRNA. Translation: BAD93930.1.
PIRC84858.
RefSeqNP_181807.1. NM_129840.4.
UniGeneAt.19848.
At.75577.

3D structure databases

ProteinModelPortalQ9SJH7.
SMRQ9SJH7. Positions 57-479.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4216. 13 interactions.
IntActQ9SJH7. 17 interactions.
STRING3702.AT2G42790.1-P.

Proteomic databases

PaxDbQ9SJH7.
PRIDEQ9SJH7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G42790.1; AT2G42790.1; AT2G42790.
GeneID818879.
KEGGath:AT2G42790.

Organism-specific databases

TAIRAT2G42790.

Phylogenomic databases

eggNOGCOG0372.
HOGENOMHOG000021224.
InParanoidQ9SJH7.
KOK01647.
OMAVERIPEF.
PhylomeDBQ9SJH7.
ProtClustDBPLN02456.

Enzyme and pathway databases

BioCycARA:AT2G42790-MONOMER.
UniPathwayUPA00223; UER00717.

Gene expression databases

ArrayExpressQ9SJH7.
GenevestigatorQ9SJH7.

Family and domain databases

Gene3D1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFPIRSF001369. Citrate_synth. 1 hit.
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY3_ARATH
AccessionPrimary (citable) accession number: Q9SJH7
Secondary accession number(s): Q56YQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: May 1, 2000
Last modified: February 19, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names