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Q9SJG9

- MPK20_ARATH

UniProt

Q9SJG9 - MPK20_ARATH

Protein

Mitogen-activated protein kinase 20

Gene

MPK20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541ATPPROSITE-ProRule annotation
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: TAIR
    3. protein binding Source: IntAct

    GO - Biological processi

    1. MAPK cascade Source: GOC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G42880-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 20 (EC:2.7.11.24)
    Short name:
    AtMPK20
    Short name:
    MAP kinase 20
    Gene namesi
    Name:MPK20
    Ordered Locus Names:At2g42880
    ORF Names:F7D19.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G42880.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 606606Mitogen-activated protein kinase 20PRO_0000245820Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei187 – 1871PhosphothreonineBy similarity
    Modified residuei189 – 1891PhosphotyrosineBy similarity
    Modified residuei192 – 1921PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9SJG9.
    PRIDEiQ9SJG9.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9SJG9.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MKK9Q9FX432EBI-2358896,EBI-2128545

    Protein-protein interaction databases

    IntActiQ9SJG9. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SJG9.
    SMRiQ9SJG9. Positions 7-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 316292Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi187 – 1893TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ9SJG9.
    OMAiSQAERYT.
    PhylomeDBiQ9SJG9.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SJG9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQQDNRKKNN LEMEFFSDYG DANRFKVQEV IGKGSYGVVC SAIDTLTGEK    50
    VAIKKIHDIF EHISDAARIL REIKLLRLLR HPDIVEIKHI MLPPSRREFK 100
    DIYVVFELME SDLHQVIKAN DDLTREHYQF FLYQLLRALK YIHTANVYHR 150
    DLKPKNILAN ANCKLKICDF GLARVAFNDT PTTIFWTDYV ATRWYRAPEL 200
    CGSFYSKYTP AIDIWSIGCI FAEVLMGKPL FPGKNVVHQL DLMTDLLGTP 250
    SLDTISRVRN EKARRYLTSM RKKPPIPFAQ KFPNADPLSL KLLERLLAFD 300
    PKDRPTAEEA LADPYFKGLA KVEREPSCQP ITKMEFEFER RKVTKEDIRE 350
    LISREILEYH PQLLKDHMNG ADKASFLYPS AVDQFRRQFA HLEENSGKTG 400
    PVAPLERKHA SLPRSTVIHS TAVARGGQPK LMNNTNTLNP ETTQNIPFNH 450
    ATIQAQQRNL SAAKPSTFMG PVAPFDNGRI SRDAYDPRSF IRSTNLPFSQ 500
    QSAATVAMGK QQERRTTMEP EKQARQISQY NRYAPDVAIN IDNNPFIMAR 550
    TGMNKAENIS DRIIIDTNLL QATAGIGVAA AAAAAAPGGS AHRKVGAVRY 600
    GMSKMY 606
    Length:606
    Mass (Da):68,775
    Last modified:June 1, 2002 - v2
    Checksum:iF36B44BDF6BD78A1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006931 Genomic DNA. Translation: AAD21721.2.
    CP002685 Genomic DNA. Translation: AEC10180.1.
    AF412082 mRNA. Translation: AAL06535.1.
    BT001021 mRNA. Translation: AAN46775.1.
    PIRiD84859.
    RefSeqiNP_565989.1. NM_129849.4.
    UniGeneiAt.14161.

    Genome annotation databases

    EnsemblPlantsiAT2G42880.1; AT2G42880.1; AT2G42880.
    GeneIDi818888.
    KEGGiath:AT2G42880.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006931 Genomic DNA. Translation: AAD21721.2 .
    CP002685 Genomic DNA. Translation: AEC10180.1 .
    AF412082 mRNA. Translation: AAL06535.1 .
    BT001021 mRNA. Translation: AAN46775.1 .
    PIRi D84859.
    RefSeqi NP_565989.1. NM_129849.4.
    UniGenei At.14161.

    3D structure databases

    ProteinModelPortali Q9SJG9.
    SMRi Q9SJG9. Positions 7-361.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9SJG9. 1 interaction.

    Proteomic databases

    PaxDbi Q9SJG9.
    PRIDEi Q9SJG9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G42880.1 ; AT2G42880.1 ; AT2G42880 .
    GeneIDi 818888.
    KEGGi ath:AT2G42880.

    Organism-specific databases

    GeneFarmi 849. 89.
    TAIRi AT2G42880.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q9SJG9.
    OMAi SQAERYT.
    PhylomeDBi Q9SJG9.

    Enzyme and pathway databases

    BioCyci ARA:AT2G42880-MONOMER.

    Miscellaneous databases

    PROi Q9SJG9.

    Gene expression databases

    Genevestigatori Q9SJG9.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
      MAPK group
      Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. Cited for: GENE FAMILY.

    Entry informationi

    Entry nameiMPK20_ARATH
    AccessioniPrimary (citable) accession number: Q9SJG9
    Secondary accession number(s): Q945L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3