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Q9SJG9

- MPK20_ARATH

UniProt

Q9SJG9 - MPK20_ARATH

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Protein

Mitogen-activated protein kinase 20

Gene

MPK20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR

GO - Biological processi

  1. MAPK cascade Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G42880-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 20 (EC:2.7.11.24)
Short name:
AtMPK20
Short name:
MAP kinase 20
Gene namesi
Name:MPK20
Ordered Locus Names:At2g42880
ORF Names:F7D19.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G42880.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Mitogen-activated protein kinase 20PRO_0000245820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei187 – 1871PhosphothreonineBy similarity
Modified residuei189 – 1891PhosphotyrosineBy similarity
Modified residuei192 – 1921PhosphothreonineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SJG9.
PRIDEiQ9SJG9.

Expressioni

Gene expression databases

GenevestigatoriQ9SJG9.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MKK9Q9FX432EBI-2358896,EBI-2128545

Protein-protein interaction databases

IntActiQ9SJG9. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9SJG9.
SMRiQ9SJG9. Positions 18-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 316292Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 1893TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9SJG9.
OMAiSQAERYT.
PhylomeDBiQ9SJG9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SJG9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQQDNRKKNN LEMEFFSDYG DANRFKVQEV IGKGSYGVVC SAIDTLTGEK
60 70 80 90 100
VAIKKIHDIF EHISDAARIL REIKLLRLLR HPDIVEIKHI MLPPSRREFK
110 120 130 140 150
DIYVVFELME SDLHQVIKAN DDLTREHYQF FLYQLLRALK YIHTANVYHR
160 170 180 190 200
DLKPKNILAN ANCKLKICDF GLARVAFNDT PTTIFWTDYV ATRWYRAPEL
210 220 230 240 250
CGSFYSKYTP AIDIWSIGCI FAEVLMGKPL FPGKNVVHQL DLMTDLLGTP
260 270 280 290 300
SLDTISRVRN EKARRYLTSM RKKPPIPFAQ KFPNADPLSL KLLERLLAFD
310 320 330 340 350
PKDRPTAEEA LADPYFKGLA KVEREPSCQP ITKMEFEFER RKVTKEDIRE
360 370 380 390 400
LISREILEYH PQLLKDHMNG ADKASFLYPS AVDQFRRQFA HLEENSGKTG
410 420 430 440 450
PVAPLERKHA SLPRSTVIHS TAVARGGQPK LMNNTNTLNP ETTQNIPFNH
460 470 480 490 500
ATIQAQQRNL SAAKPSTFMG PVAPFDNGRI SRDAYDPRSF IRSTNLPFSQ
510 520 530 540 550
QSAATVAMGK QQERRTTMEP EKQARQISQY NRYAPDVAIN IDNNPFIMAR
560 570 580 590 600
TGMNKAENIS DRIIIDTNLL QATAGIGVAA AAAAAAPGGS AHRKVGAVRY

GMSKMY
Length:606
Mass (Da):68,775
Last modified:June 1, 2002 - v2
Checksum:iF36B44BDF6BD78A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006931 Genomic DNA. Translation: AAD21721.2.
CP002685 Genomic DNA. Translation: AEC10180.1.
AF412082 mRNA. Translation: AAL06535.1.
BT001021 mRNA. Translation: AAN46775.1.
PIRiD84859.
RefSeqiNP_565989.1. NM_129849.4.
UniGeneiAt.14161.

Genome annotation databases

EnsemblPlantsiAT2G42880.1; AT2G42880.1; AT2G42880.
GeneIDi818888.
KEGGiath:AT2G42880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006931 Genomic DNA. Translation: AAD21721.2 .
CP002685 Genomic DNA. Translation: AEC10180.1 .
AF412082 mRNA. Translation: AAL06535.1 .
BT001021 mRNA. Translation: AAN46775.1 .
PIRi D84859.
RefSeqi NP_565989.1. NM_129849.4.
UniGenei At.14161.

3D structure databases

ProteinModelPortali Q9SJG9.
SMRi Q9SJG9. Positions 18-362.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9SJG9. 1 interaction.

Proteomic databases

PaxDbi Q9SJG9.
PRIDEi Q9SJG9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G42880.1 ; AT2G42880.1 ; AT2G42880 .
GeneIDi 818888.
KEGGi ath:AT2G42880.

Organism-specific databases

GeneFarmi 849. 89.
TAIRi AT2G42880.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q9SJG9.
OMAi SQAERYT.
PhylomeDBi Q9SJG9.

Enzyme and pathway databases

BioCyci ARA:AT2G42880-MONOMER.

Miscellaneous databases

PROi Q9SJG9.

Gene expression databases

Genevestigatori Q9SJG9.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. Cited for: GENE FAMILY.

Entry informationi

Entry nameiMPK20_ARATH
AccessioniPrimary (citable) accession number: Q9SJG9
Secondary accession number(s): Q945L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3