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Q9SJG9 (MPK20_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 20

Short name=AtMPK20
Short name=MAP kinase 20
EC=2.7.11.24
Gene names
Name:MPK20
Ordered Locus Names:At2g42880
ORF Names:F7D19.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length606 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MKK9Q9FX432EBI-2358896,EBI-2128545

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 606606Mitogen-activated protein kinase 20
PRO_0000245820

Regions

Domain25 – 316292Protein kinase
Nucleotide binding31 – 399ATP By similarity
Motif187 – 1893TXY

Sites

Active site1511Proton acceptor By similarity
Binding site541ATP By similarity

Amino acid modifications

Modified residue1871Phosphothreonine By similarity
Modified residue1891Phosphotyrosine By similarity
Modified residue1921Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9SJG9 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: F36B44BDF6BD78A1

FASTA60668,775
        10         20         30         40         50         60 
MQQDNRKKNN LEMEFFSDYG DANRFKVQEV IGKGSYGVVC SAIDTLTGEK VAIKKIHDIF 

        70         80         90        100        110        120 
EHISDAARIL REIKLLRLLR HPDIVEIKHI MLPPSRREFK DIYVVFELME SDLHQVIKAN 

       130        140        150        160        170        180 
DDLTREHYQF FLYQLLRALK YIHTANVYHR DLKPKNILAN ANCKLKICDF GLARVAFNDT 

       190        200        210        220        230        240 
PTTIFWTDYV ATRWYRAPEL CGSFYSKYTP AIDIWSIGCI FAEVLMGKPL FPGKNVVHQL 

       250        260        270        280        290        300 
DLMTDLLGTP SLDTISRVRN EKARRYLTSM RKKPPIPFAQ KFPNADPLSL KLLERLLAFD 

       310        320        330        340        350        360 
PKDRPTAEEA LADPYFKGLA KVEREPSCQP ITKMEFEFER RKVTKEDIRE LISREILEYH 

       370        380        390        400        410        420 
PQLLKDHMNG ADKASFLYPS AVDQFRRQFA HLEENSGKTG PVAPLERKHA SLPRSTVIHS 

       430        440        450        460        470        480 
TAVARGGQPK LMNNTNTLNP ETTQNIPFNH ATIQAQQRNL SAAKPSTFMG PVAPFDNGRI 

       490        500        510        520        530        540 
SRDAYDPRSF IRSTNLPFSQ QSAATVAMGK QQERRTTMEP EKQARQISQY NRYAPDVAIN 

       550        560        570        580        590        600 
IDNNPFIMAR TGMNKAENIS DRIIIDTNLL QATAGIGVAA AAAAAAPGGS AHRKVGAVRY 


GMSKMY 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Mitogen-activated protein kinase cascades in plants: a new nomenclature."
MAPK group
Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Ancient signals: comparative genomics of plant MAPK and MAPKK gene families."
Hamel L.P., Nicole M.C., Sritubtim S., Morency M.J., Ellis M., Ehlting J., Beaudoin N., Barbazuk B., Klessig D., Lee J., Martin G., Mundy J., Ohashi Y., Scheel D., Sheen J., Xing T., Zhang S., Seguin A., Ellis B.E.
Trends Plant Sci. 11:192-198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006931 Genomic DNA. Translation: AAD21721.2.
CP002685 Genomic DNA. Translation: AEC10180.1.
AF412082 mRNA. Translation: AAL06535.1.
BT001021 mRNA. Translation: AAN46775.1.
PIRD84859.
RefSeqNP_565989.1. NM_129849.4.
UniGeneAt.14161.

3D structure databases

ProteinModelPortalQ9SJG9.
SMRQ9SJG9. Positions 7-361.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9SJG9. 1 interaction.

Proteomic databases

PaxDbQ9SJG9.
PRIDEQ9SJG9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G42880.1; AT2G42880.1; AT2G42880.
GeneID818888.
KEGGath:AT2G42880.

Organism-specific databases

GeneFarm849. 89.
TAIRAT2G42880.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ9SJG9.
OMASQAERYT.
PhylomeDBQ9SJG9.

Enzyme and pathway databases

BioCycARA:AT2G42880-MONOMER.

Gene expression databases

GenevestigatorQ9SJG9.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9SJG9.

Entry information

Entry nameMPK20_ARATH
AccessionPrimary (citable) accession number: Q9SJG9
Secondary accession number(s): Q945L8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names