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Q9SJF0

- BSL2_ARATH

UniProt

Q9SJF0 - BSL2_ARATH

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Protein

Serine/threonine-protein phosphatase BSL2

Gene

BSL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi720 – 7201Manganese 1By similarity
Metal bindingi722 – 7221Manganese 1By similarity
Metal bindingi754 – 7541Manganese 1By similarity
Metal bindingi754 – 7541Manganese 2By similarity
Metal bindingi786 – 7861Manganese 2By similarity
Active sitei787 – 7871Proton donorBy similarity
Metal bindingi839 – 8391Manganese 2By similarity
Metal bindingi918 – 9181Manganese 2By similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. manganese ion binding Source: InterPro
  3. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G08420-MONOMER.
ARA:GQT-182-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase BSL2 (EC:3.1.3.16)
Alternative name(s):
BSU1-like protein 2
Gene namesi
Name:BSL2
Ordered Locus Names:At1g08420
ORF Names:T27G7.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G08420.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10181018Serine/threonine-protein phosphatase BSL2PRO_0000058906Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei627 – 6271PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SJF0.
PRIDEiQ9SJF0.

Expressioni

Tissue specificityi

Expressed throughout the plant, with a higher level in younger parts.1 Publication

Gene expression databases

ExpressionAtlasiQ9SJF0. baseline.
GenevestigatoriQ9SJF0.

Interactioni

Protein-protein interaction databases

BioGridi22603. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9SJF0.
SMRiQ9SJF0. Positions 666-968.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati149 – 19547Kelch 1Add
BLAST
Repeati253 – 30149Kelch 2Add
BLAST
Repeati306 – 35651Kelch 3Add
BLAST
Repeati362 – 40948Kelch 4Add
BLAST
Repeati430 – 47950Kelch 5Add
BLAST

Sequence similaritiesi

Belongs to the PPP phosphatase family. BSU subfamily.Curated
Contains 5 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000246464.
InParanoidiQ9SJF0.
OMAiERETPQQ.
PhylomeDBiQ9SJF0.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view]
PfamiPF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SJF0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEDSSMVAD NDQDREFQSL DGGQSPSPME RETPQQMNDQ SPPPEGGSVP
60 70 80 90 100
TPPPSDPNPA TSQQQAAAVV GQEQQPALVV GPRCAPTYSV VDAMMDKKED
110 120 130 140 150
GPGPRCGHTL TAVPAVGDEG TPGYIGPRLV LFGGATALEG NSGGTGTPTS
160 170 180 190 200
AGSAGIRLAG ATADVHCYDV LSNKWTRLTP FGEPPTPRAA HVATAVGTMV
210 220 230 240 250
VIQGGIGPAG LSAEDLHVLD LTQQRPRWHR VVVQGPGPGP RYGHVMALVG
260 270 280 290 300
QRYLMAIGGN DGKRPLADVW ALDTAAKPYE WRKLEPEGEG PPPCMYATAS
310 320 330 340 350
ARSDGLLLLC GGRDANSVPL ASAYGLAKHR DGRWEWAIAP GVSPSSRYQH
360 370 380 390 400
AAVFVNARLH VSGGALGGGR MVEDSSSVAV LDTAAGVWCD TKSVVTSPRT
410 420 430 440 450
GRYSADAAGG DASVELTRRC RHAAAAVGDL IFIYGGLRGG VLLDDLLVAE
460 470 480 490 500
DLAAAETTYA ASHAAAAAAT NSPPGRLPGR YGFSDERNRE LSESAADGAV
510 520 530 540 550
VLGSPVAPPV NGDMHTDISP ENALLPGTRR TNKGVEYLVE ASAAEAEAIS
560 570 580 590 600
ATLAAAKARQ VNGEVELPDR DCGAEATPSG KPTFSLIKPD SMGSMSVTPA
610 620 630 640 650
GIRLHHRAVV VAAETGGALG GMVRQLSIDQ FENEGRRVSY GTPESATAAR
660 670 680 690 700
KLLDRQMSIN SVPKKVIAHL LKPRGWKPPV RRQFFLDCNE IADLCDSAER
710 720 730 740 750
IFASEPTVLQ LKAPIKIFGD LHGQFGDLMR LFDEYGSPST AGDISYIDYL
760 770 780 790 800
FLGDYVDRGQ HSLETISLLL ALKVEYQHNV HLIRGNHEAA DINALFGFRI
810 820 830 840 850
ECIERMGERD GIWVWHRINR LFNWLPLAAS IEKKIICMHG GIGRSINHVE
860 870 880 890 900
QIENIQRPIT MEAGSIVLMD LLWSDPTEND SVEGLRPNAR GPGLVTFGPD
910 920 930 940 950
RVMEFCNNND LQLIVRAHEC VMDGFERFAQ GHLITLFSAT NYCGTANNAG
960 970 980 990 1000
AILVLGRDLV VVPKLIHPLP PALSSPETSP ERHIEDTWMQ ELNANRPATP
1010
TRGRPQNSND RGGSLAWM
Length:1,018
Mass (Da):108,580
Last modified:October 23, 2007 - v2
Checksum:iF2D48037DF4FAAB4
GO

Sequence cautioni

The sequence AAF22889.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006932 Genomic DNA. Translation: AAF22889.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28287.1.
AK230437 mRNA. Translation: BAF02235.1.
PIRiE86217.
RefSeqiNP_172318.1. NM_100715.3. [Q9SJF0-1]
UniGeneiAt.27687.

Genome annotation databases

EnsemblPlantsiAT1G08420.1; AT1G08420.1; AT1G08420. [Q9SJF0-1]
GeneIDi837362.
KEGGiath:AT1G08420.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006932 Genomic DNA. Translation: AAF22889.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE28287.1 .
AK230437 mRNA. Translation: BAF02235.1 .
PIRi E86217.
RefSeqi NP_172318.1. NM_100715.3. [Q9SJF0-1 ]
UniGenei At.27687.

3D structure databases

ProteinModelPortali Q9SJF0.
SMRi Q9SJF0. Positions 666-968.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 22603. 1 interaction.

Proteomic databases

PaxDbi Q9SJF0.
PRIDEi Q9SJF0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G08420.1 ; AT1G08420.1 ; AT1G08420 . [Q9SJF0-1 ]
GeneIDi 837362.
KEGGi ath:AT1G08420.

Organism-specific databases

TAIRi AT1G08420.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000246464.
InParanoidi Q9SJF0.
OMAi ERETPQQ.
PhylomeDBi Q9SJF0.

Enzyme and pathway databases

BioCyci ARA:AT1G08420-MONOMER.
ARA:GQT-182-MONOMER.

Gene expression databases

ExpressionAtlasi Q9SJF0. baseline.
Genevestigatori Q9SJF0.

Family and domain databases

Gene3Di 2.120.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view ]
Pfami PF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PIRSFi PIRSF036363. PPP_BSU1. 1 hit.
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 282-1018.
    Strain: cv. Columbia.
  4. "Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis."
    Mora-Garcia S., Vert G., Yin Y., Cano-Delgado A., Cheong H., Chory J.
    Genes Dev. 18:448-460(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBSL2_ARATH
AccessioniPrimary (citable) accession number: Q9SJF0
Secondary accession number(s): Q0WKX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: October 23, 2007
Last modified: October 29, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3