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Q9SJF0

- BSL2_ARATH

UniProt

Q9SJF0 - BSL2_ARATH

Protein

Serine/threonine-protein phosphatase BSL2

Gene

BSL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi720 – 7201Manganese 1By similarity
    Metal bindingi722 – 7221Manganese 1By similarity
    Metal bindingi754 – 7541Manganese 1By similarity
    Metal bindingi754 – 7541Manganese 2By similarity
    Metal bindingi786 – 7861Manganese 2By similarity
    Active sitei787 – 7871Proton donorBy similarity
    Metal bindingi839 – 8391Manganese 2By similarity
    Metal bindingi918 – 9181Manganese 2By similarity

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. manganese ion binding Source: InterPro
    3. phosphoprotein phosphatase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G08420-MONOMER.
    ARA:GQT-182-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase BSL2 (EC:3.1.3.16)
    Alternative name(s):
    BSU1-like protein 2
    Gene namesi
    Name:BSL2
    Ordered Locus Names:At1g08420
    ORF Names:T27G7.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G08420.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10181018Serine/threonine-protein phosphatase BSL2PRO_0000058906Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei627 – 6271PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9SJF0.
    PRIDEiQ9SJF0.

    Expressioni

    Tissue specificityi

    Expressed throughout the plant, with a higher level in younger parts.1 Publication

    Gene expression databases

    GenevestigatoriQ9SJF0.

    Interactioni

    Protein-protein interaction databases

    BioGridi22603. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SJF0.
    SMRiQ9SJF0. Positions 666-968.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati149 – 19547Kelch 1Add
    BLAST
    Repeati253 – 30149Kelch 2Add
    BLAST
    Repeati306 – 35651Kelch 3Add
    BLAST
    Repeati362 – 40948Kelch 4Add
    BLAST
    Repeati430 – 47950Kelch 5Add
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. BSU subfamily.Curated
    Contains 5 Kelch repeats.Curated

    Keywords - Domaini

    Kelch repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000246464.
    InParanoidiQ9SJF0.
    OMAiERETPQQ.
    PhylomeDBiQ9SJF0.

    Family and domain databases

    Gene3Di2.120.10.80. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR015915. Kelch-typ_b-propeller.
    IPR011498. Kelch_2.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    IPR012391. Ser/Thr_prot_Pase_BSU1.
    [Graphical view]
    PfamiPF07646. Kelch_2. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9SJF0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDEDSSMVAD NDQDREFQSL DGGQSPSPME RETPQQMNDQ SPPPEGGSVP     50
    TPPPSDPNPA TSQQQAAAVV GQEQQPALVV GPRCAPTYSV VDAMMDKKED 100
    GPGPRCGHTL TAVPAVGDEG TPGYIGPRLV LFGGATALEG NSGGTGTPTS 150
    AGSAGIRLAG ATADVHCYDV LSNKWTRLTP FGEPPTPRAA HVATAVGTMV 200
    VIQGGIGPAG LSAEDLHVLD LTQQRPRWHR VVVQGPGPGP RYGHVMALVG 250
    QRYLMAIGGN DGKRPLADVW ALDTAAKPYE WRKLEPEGEG PPPCMYATAS 300
    ARSDGLLLLC GGRDANSVPL ASAYGLAKHR DGRWEWAIAP GVSPSSRYQH 350
    AAVFVNARLH VSGGALGGGR MVEDSSSVAV LDTAAGVWCD TKSVVTSPRT 400
    GRYSADAAGG DASVELTRRC RHAAAAVGDL IFIYGGLRGG VLLDDLLVAE 450
    DLAAAETTYA ASHAAAAAAT NSPPGRLPGR YGFSDERNRE LSESAADGAV 500
    VLGSPVAPPV NGDMHTDISP ENALLPGTRR TNKGVEYLVE ASAAEAEAIS 550
    ATLAAAKARQ VNGEVELPDR DCGAEATPSG KPTFSLIKPD SMGSMSVTPA 600
    GIRLHHRAVV VAAETGGALG GMVRQLSIDQ FENEGRRVSY GTPESATAAR 650
    KLLDRQMSIN SVPKKVIAHL LKPRGWKPPV RRQFFLDCNE IADLCDSAER 700
    IFASEPTVLQ LKAPIKIFGD LHGQFGDLMR LFDEYGSPST AGDISYIDYL 750
    FLGDYVDRGQ HSLETISLLL ALKVEYQHNV HLIRGNHEAA DINALFGFRI 800
    ECIERMGERD GIWVWHRINR LFNWLPLAAS IEKKIICMHG GIGRSINHVE 850
    QIENIQRPIT MEAGSIVLMD LLWSDPTEND SVEGLRPNAR GPGLVTFGPD 900
    RVMEFCNNND LQLIVRAHEC VMDGFERFAQ GHLITLFSAT NYCGTANNAG 950
    AILVLGRDLV VVPKLIHPLP PALSSPETSP ERHIEDTWMQ ELNANRPATP 1000
    TRGRPQNSND RGGSLAWM 1018
    Length:1,018
    Mass (Da):108,580
    Last modified:October 23, 2007 - v2
    Checksum:iF2D48037DF4FAAB4
    GO

    Sequence cautioni

    The sequence AAF22889.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006932 Genomic DNA. Translation: AAF22889.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28287.1.
    AK230437 mRNA. Translation: BAF02235.1.
    PIRiE86217.
    RefSeqiNP_172318.1. NM_100715.3. [Q9SJF0-1]
    UniGeneiAt.27687.

    Genome annotation databases

    EnsemblPlantsiAT1G08420.1; AT1G08420.1; AT1G08420. [Q9SJF0-1]
    GeneIDi837362.
    KEGGiath:AT1G08420.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006932 Genomic DNA. Translation: AAF22889.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28287.1 .
    AK230437 mRNA. Translation: BAF02235.1 .
    PIRi E86217.
    RefSeqi NP_172318.1. NM_100715.3. [Q9SJF0-1 ]
    UniGenei At.27687.

    3D structure databases

    ProteinModelPortali Q9SJF0.
    SMRi Q9SJF0. Positions 666-968.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 22603. 1 interaction.

    Proteomic databases

    PaxDbi Q9SJF0.
    PRIDEi Q9SJF0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G08420.1 ; AT1G08420.1 ; AT1G08420 . [Q9SJF0-1 ]
    GeneIDi 837362.
    KEGGi ath:AT1G08420.

    Organism-specific databases

    TAIRi AT1G08420.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000246464.
    InParanoidi Q9SJF0.
    OMAi ERETPQQ.
    PhylomeDBi Q9SJF0.

    Enzyme and pathway databases

    BioCyci ARA:AT1G08420-MONOMER.
    ARA:GQT-182-MONOMER.

    Gene expression databases

    Genevestigatori Q9SJF0.

    Family and domain databases

    Gene3Di 2.120.10.80. 1 hit.
    3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR015915. Kelch-typ_b-propeller.
    IPR011498. Kelch_2.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    IPR012391. Ser/Thr_prot_Pase_BSU1.
    [Graphical view ]
    Pfami PF07646. Kelch_2. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036363. PPP_BSU1. 1 hit.
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 282-1018.
      Strain: cv. Columbia.
    4. "Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis."
      Mora-Garcia S., Vert G., Yin Y., Cano-Delgado A., Cheong H., Chory J.
      Genes Dev. 18:448-460(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    5. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBSL2_ARATH
    AccessioniPrimary (citable) accession number: Q9SJF0
    Secondary accession number(s): Q0WKX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2004
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3