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Protein

Serine/threonine-protein phosphatase BSL2

Gene

BSL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi720Manganese 1By similarity1
Metal bindingi722Manganese 1By similarity1
Metal bindingi754Manganese 1By similarity1
Metal bindingi754Manganese 2By similarity1
Metal bindingi786Manganese 2By similarity1
Active sitei787Proton donorBy similarity1
Metal bindingi839Manganese 2By similarity1
Metal bindingi918Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase BSL2 (EC:3.1.3.16)
Alternative name(s):
BSU1-like protein 2
Gene namesi
Name:BSL2
Ordered Locus Names:At1g08420
ORF Names:T27G7.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G08420.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000589061 – 1018Serine/threonine-protein phosphatase BSL2Add BLAST1018

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei627PhosphoserineBy similarity1
Modified residuei975PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SJF0.
PRIDEiQ9SJF0.

PTM databases

iPTMnetiQ9SJF0.

Expressioni

Tissue specificityi

Expressed throughout the plant, with a higher level in younger parts.1 Publication

Gene expression databases

ExpressionAtlasiQ9SJF0. differential.
GenevisibleiQ9SJF0. AT.

Interactioni

Protein-protein interaction databases

BioGridi22603. 3 interactors.
STRINGi3702.AT1G08420.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SJF0.
SMRiQ9SJF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati149 – 195Kelch 1Add BLAST47
Repeati253 – 301Kelch 2Add BLAST49
Repeati306 – 356Kelch 3Add BLAST51
Repeati362 – 409Kelch 4Add BLAST48
Repeati430 – 479Kelch 5Add BLAST50

Sequence similaritiesi

Belongs to the PPP phosphatase family. BSU subfamily.Curated
Contains 5 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
KOG0379. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000246464.
InParanoidiQ9SJF0.
OMAiMVADNDQ.
OrthoDBiEOG093601IG.
PhylomeDBiQ9SJF0.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view]
PfamiPF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SJF0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEDSSMVAD NDQDREFQSL DGGQSPSPME RETPQQMNDQ SPPPEGGSVP
60 70 80 90 100
TPPPSDPNPA TSQQQAAAVV GQEQQPALVV GPRCAPTYSV VDAMMDKKED
110 120 130 140 150
GPGPRCGHTL TAVPAVGDEG TPGYIGPRLV LFGGATALEG NSGGTGTPTS
160 170 180 190 200
AGSAGIRLAG ATADVHCYDV LSNKWTRLTP FGEPPTPRAA HVATAVGTMV
210 220 230 240 250
VIQGGIGPAG LSAEDLHVLD LTQQRPRWHR VVVQGPGPGP RYGHVMALVG
260 270 280 290 300
QRYLMAIGGN DGKRPLADVW ALDTAAKPYE WRKLEPEGEG PPPCMYATAS
310 320 330 340 350
ARSDGLLLLC GGRDANSVPL ASAYGLAKHR DGRWEWAIAP GVSPSSRYQH
360 370 380 390 400
AAVFVNARLH VSGGALGGGR MVEDSSSVAV LDTAAGVWCD TKSVVTSPRT
410 420 430 440 450
GRYSADAAGG DASVELTRRC RHAAAAVGDL IFIYGGLRGG VLLDDLLVAE
460 470 480 490 500
DLAAAETTYA ASHAAAAAAT NSPPGRLPGR YGFSDERNRE LSESAADGAV
510 520 530 540 550
VLGSPVAPPV NGDMHTDISP ENALLPGTRR TNKGVEYLVE ASAAEAEAIS
560 570 580 590 600
ATLAAAKARQ VNGEVELPDR DCGAEATPSG KPTFSLIKPD SMGSMSVTPA
610 620 630 640 650
GIRLHHRAVV VAAETGGALG GMVRQLSIDQ FENEGRRVSY GTPESATAAR
660 670 680 690 700
KLLDRQMSIN SVPKKVIAHL LKPRGWKPPV RRQFFLDCNE IADLCDSAER
710 720 730 740 750
IFASEPTVLQ LKAPIKIFGD LHGQFGDLMR LFDEYGSPST AGDISYIDYL
760 770 780 790 800
FLGDYVDRGQ HSLETISLLL ALKVEYQHNV HLIRGNHEAA DINALFGFRI
810 820 830 840 850
ECIERMGERD GIWVWHRINR LFNWLPLAAS IEKKIICMHG GIGRSINHVE
860 870 880 890 900
QIENIQRPIT MEAGSIVLMD LLWSDPTEND SVEGLRPNAR GPGLVTFGPD
910 920 930 940 950
RVMEFCNNND LQLIVRAHEC VMDGFERFAQ GHLITLFSAT NYCGTANNAG
960 970 980 990 1000
AILVLGRDLV VVPKLIHPLP PALSSPETSP ERHIEDTWMQ ELNANRPATP
1010
TRGRPQNSND RGGSLAWM
Length:1,018
Mass (Da):108,580
Last modified:October 23, 2007 - v2
Checksum:iF2D48037DF4FAAB4
GO

Sequence cautioni

The sequence AAF22889 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006932 Genomic DNA. Translation: AAF22889.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28287.1.
AK230437 mRNA. Translation: BAF02235.1.
PIRiE86217.
RefSeqiNP_172318.1. NM_100715.4. [Q9SJF0-1]
UniGeneiAt.27687.

Genome annotation databases

EnsemblPlantsiAT1G08420.1; AT1G08420.1; AT1G08420. [Q9SJF0-1]
GeneIDi837362.
GrameneiAT1G08420.1; AT1G08420.1; AT1G08420.
KEGGiath:AT1G08420.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006932 Genomic DNA. Translation: AAF22889.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28287.1.
AK230437 mRNA. Translation: BAF02235.1.
PIRiE86217.
RefSeqiNP_172318.1. NM_100715.4. [Q9SJF0-1]
UniGeneiAt.27687.

3D structure databases

ProteinModelPortaliQ9SJF0.
SMRiQ9SJF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22603. 3 interactors.
STRINGi3702.AT1G08420.1.

PTM databases

iPTMnetiQ9SJF0.

Proteomic databases

PaxDbiQ9SJF0.
PRIDEiQ9SJF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G08420.1; AT1G08420.1; AT1G08420. [Q9SJF0-1]
GeneIDi837362.
GrameneiAT1G08420.1; AT1G08420.1; AT1G08420.
KEGGiath:AT1G08420.

Organism-specific databases

TAIRiAT1G08420.

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
KOG0379. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000246464.
InParanoidiQ9SJF0.
OMAiMVADNDQ.
OrthoDBiEOG093601IG.
PhylomeDBiQ9SJF0.

Miscellaneous databases

PROiQ9SJF0.

Gene expression databases

ExpressionAtlasiQ9SJF0. differential.
GenevisibleiQ9SJF0. AT.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view]
PfamiPF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBSL2_ARATH
AccessioniPrimary (citable) accession number: Q9SJF0
Secondary accession number(s): Q0WKX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: October 23, 2007
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.