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Q9SJD4

- LACS8_ARATH

UniProt

Q9SJD4 - LACS8_ARATH

Protein

Long chain acyl-CoA synthetase 8

Gene

LACS8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate and linoleate.

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi279 – 29012ATPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: UniProtKB

    GO - Biological processi

    1. fatty acid metabolic process Source: UniProtKB
    2. long-chain fatty acid metabolic process Source: GOC

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G04350-MONOMER.
    ARA:GQT-1997-MONOMER.
    MetaCyc:AT2G04350-MONOMER.
    SABIO-RKQ9SJD4.
    UniPathwayiUPA00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long chain acyl-CoA synthetase 8 (EC:6.2.1.3)
    Gene namesi
    Name:LACS8
    Ordered Locus Names:At2g04350
    ORF Names:T1O3, T23O15.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G04350.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast envelope Source: TAIR
    2. endoplasmic reticulum Source: TAIR
    3. Golgi apparatus Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 720720Long chain acyl-CoA synthetase 8PRO_0000401417Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ9SJD4.
    PRIDEiQ9SJD4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9SJD4.

    Interactioni

    Protein-protein interaction databases

    BioGridi375. 3 interactions.
    STRINGi3702.AT2G04350.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SJD4.
    SMRiQ9SJD4. Positions 116-711.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni554 – 58229Fatty acid-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    HOGENOMiHOG000159459.
    InParanoidiQ9SJD4.
    KOiK01897.
    OMAiNRACNFA.
    PhylomeDBiQ9SJD4.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SJD4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDSGVNPMD SPSKGSDFGV YGIIGGGIVA LLVPVLLSVV LNGTKKGKKR    50
    GVPIKVGGEE GYTMRHARAP ELVDVPWEGA ATMPALFEQS CKKYSKDRLL 100
    GTREFIDKEF ITASDGRKFE KLHLGEYKWQ SYGEVFERVC NFASGLVNVG 150
    HNVDDRVAIF SDTRAEWFIA FQGCFRQSIT VVTIYASLGE EALIYSLNET 200
    RVSTLICDSK QLKKLSAIQS SLKTVKNIIY IEEDGVDVAS SDVNSMGDIT 250
    VSSISEVEKL GQKNAVQPIL PSKNGVAVIM FTSGSTGLPK GVMITHGNLV 300
    ATAAGVMKVV PKLDKNDTYI AYLPLAHVFE LEAEIVVFTS GSAIGYGSAM 350
    TLTDTSNKVK KGTKGDVSAL KPTIMTAVPA ILDRVREGVL KKVEEKGGMA 400
    KTLFDFAYKR RLAAVDGSWF GAWGLEKMLW DALVFKKIRA VLGGHIRFML 450
    VGGAPLSPDS QRFINICMGS PIGQGYGLTE TCAGATFSEW DDPAVGRVGP 500
    PLPCGYVKLV SWEEGGYRIS DKPMPRGEIV VGGNSVTAGY FNNQEKTDEV 550
    YKVDEKGTRW FYTGDIGRFH PDGCLEVIDR KKDIVKLQHG EYVSLGKVEA 600
    ALGSSNYVDN IMVHADPINS YCVALVVPSR GALEKWAEEA GVKHSEFAEL 650
    CEKGEAVKEV QQSLTKAGKA AKLEKFELPA KIKLLSEPWT PESGLVTAAL 700
    KIKREQIKSK FKDELSKLYA 720
    Length:720
    Mass (Da):78,343
    Last modified:May 1, 2000 - v1
    Checksum:i96ED3FD23AD2ED75
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti256 – 2638EVEKLGQK → DFFKLPPH in AAK96568. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503758 mRNA. Translation: AAM28875.1.
    AC006951 Genomic DNA. Translation: AAD25843.1.
    AC007213 Genomic DNA. Translation: AAM15458.1.
    CP002685 Genomic DNA. Translation: AEC05822.1.
    CP002685 Genomic DNA. Translation: AEC05823.1.
    AY052664 mRNA. Translation: AAK96568.1.
    BT002288 mRNA. Translation: AAN72299.1.
    PIRiE84456.
    RefSeqiNP_178516.1. NM_126468.3.
    NP_849934.1. NM_179603.2.
    UniGeneiAt.21686.

    Genome annotation databases

    EnsemblPlantsiAT2G04350.1; AT2G04350.1; AT2G04350.
    AT2G04350.2; AT2G04350.2; AT2G04350.
    GeneIDi814974.
    KEGGiath:AT2G04350.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503758 mRNA. Translation: AAM28875.1 .
    AC006951 Genomic DNA. Translation: AAD25843.1 .
    AC007213 Genomic DNA. Translation: AAM15458.1 .
    CP002685 Genomic DNA. Translation: AEC05822.1 .
    CP002685 Genomic DNA. Translation: AEC05823.1 .
    AY052664 mRNA. Translation: AAK96568.1 .
    BT002288 mRNA. Translation: AAN72299.1 .
    PIRi E84456.
    RefSeqi NP_178516.1. NM_126468.3.
    NP_849934.1. NM_179603.2.
    UniGenei At.21686.

    3D structure databases

    ProteinModelPortali Q9SJD4.
    SMRi Q9SJD4. Positions 116-711.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 375. 3 interactions.
    STRINGi 3702.AT2G04350.1-P.

    Proteomic databases

    PaxDbi Q9SJD4.
    PRIDEi Q9SJD4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G04350.1 ; AT2G04350.1 ; AT2G04350 .
    AT2G04350.2 ; AT2G04350.2 ; AT2G04350 .
    GeneIDi 814974.
    KEGGi ath:AT2G04350.

    Organism-specific databases

    TAIRi AT2G04350.

    Phylogenomic databases

    eggNOGi COG1022.
    HOGENOMi HOG000159459.
    InParanoidi Q9SJD4.
    KOi K01897.
    OMAi NRACNFA.
    PhylomeDBi Q9SJD4.

    Enzyme and pathway databases

    UniPathwayi UPA00199 .
    BioCyci ARA:AT2G04350-MONOMER.
    ARA:GQT-1997-MONOMER.
    MetaCyc:AT2G04350-MONOMER.
    SABIO-RK Q9SJD4.

    Gene expression databases

    Genevestigatori Q9SJD4.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
      Shockey J.M., Fulda M.S., Browse J.A.
      Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
      Shockey J.M., Fulda M.S., Browse J.
      Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY ORGANIZATION.
    6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLACS8_ARATH
    AccessioniPrimary (citable) accession number: Q9SJD4
    Secondary accession number(s): Q940V0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3