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Protein

Long chain acyl-CoA synthetase 8

Gene

LACS8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate and linoleate.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

Cofactori

Mg2+By similarity

Pathwayi: fatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi279 – 290ATPSequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • fatty acid metabolic process Source: UniProtKB

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G04350-MONOMER
MetaCyc:AT2G04350-MONOMER
ReactomeiR-ATH-75876 Synthesis of very long-chain fatty acyl-CoAs
SABIO-RKQ9SJD4
UniPathwayiUPA00199

Names & Taxonomyi

Protein namesi
Recommended name:
Long chain acyl-CoA synthetase 8 (EC:6.2.1.3)
Gene namesi
Name:LACS8
Ordered Locus Names:At2g04350
ORF Names:T1O3, T23O15.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G04350
TAIRilocus:2058384 AT2G04350

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004014171 – 720Long chain acyl-CoA synthetase 8Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9SJD4
PRIDEiQ9SJD4

PTM databases

iPTMnetiQ9SJD4

Expressioni

Gene expression databases

ExpressionAtlasiQ9SJD4 baseline and differential
GenevisibleiQ9SJD4 AT

Interactioni

Protein-protein interaction databases

BioGridi375, 3 interactors
STRINGi3702.AT2G04350.1

Structurei

3D structure databases

ProteinModelPortaliQ9SJD4
SMRiQ9SJD4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni554 – 582Fatty acid-bindingSequence analysisAdd BLAST29

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1180 Eukaryota
COG1022 LUCA
HOGENOMiHOG000159459
InParanoidiQ9SJD4
KOiK01897
OMAiQEMNYIQ
OrthoDBiEOG093604JZ
PhylomeDBiQ9SJD4

Family and domain databases

InterProiView protein in InterPro
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SJD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDSGVNPMD SPSKGSDFGV YGIIGGGIVA LLVPVLLSVV LNGTKKGKKR
60 70 80 90 100
GVPIKVGGEE GYTMRHARAP ELVDVPWEGA ATMPALFEQS CKKYSKDRLL
110 120 130 140 150
GTREFIDKEF ITASDGRKFE KLHLGEYKWQ SYGEVFERVC NFASGLVNVG
160 170 180 190 200
HNVDDRVAIF SDTRAEWFIA FQGCFRQSIT VVTIYASLGE EALIYSLNET
210 220 230 240 250
RVSTLICDSK QLKKLSAIQS SLKTVKNIIY IEEDGVDVAS SDVNSMGDIT
260 270 280 290 300
VSSISEVEKL GQKNAVQPIL PSKNGVAVIM FTSGSTGLPK GVMITHGNLV
310 320 330 340 350
ATAAGVMKVV PKLDKNDTYI AYLPLAHVFE LEAEIVVFTS GSAIGYGSAM
360 370 380 390 400
TLTDTSNKVK KGTKGDVSAL KPTIMTAVPA ILDRVREGVL KKVEEKGGMA
410 420 430 440 450
KTLFDFAYKR RLAAVDGSWF GAWGLEKMLW DALVFKKIRA VLGGHIRFML
460 470 480 490 500
VGGAPLSPDS QRFINICMGS PIGQGYGLTE TCAGATFSEW DDPAVGRVGP
510 520 530 540 550
PLPCGYVKLV SWEEGGYRIS DKPMPRGEIV VGGNSVTAGY FNNQEKTDEV
560 570 580 590 600
YKVDEKGTRW FYTGDIGRFH PDGCLEVIDR KKDIVKLQHG EYVSLGKVEA
610 620 630 640 650
ALGSSNYVDN IMVHADPINS YCVALVVPSR GALEKWAEEA GVKHSEFAEL
660 670 680 690 700
CEKGEAVKEV QQSLTKAGKA AKLEKFELPA KIKLLSEPWT PESGLVTAAL
710 720
KIKREQIKSK FKDELSKLYA
Length:720
Mass (Da):78,343
Last modified:May 1, 2000 - v1
Checksum:i96ED3FD23AD2ED75
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256 – 263EVEKLGQK → DFFKLPPH in AAK96568 (PubMed:14593172).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503758 mRNA Translation: AAM28875.1
AC006951 Genomic DNA Translation: AAD25843.1
AC007213 Genomic DNA Translation: AAM15458.1
CP002685 Genomic DNA Translation: AEC05822.1
CP002685 Genomic DNA Translation: AEC05823.1
AY052664 mRNA Translation: AAK96568.1
BT002288 mRNA Translation: AAN72299.1
PIRiE84456
RefSeqiNP_178516.1, NM_126468.4
NP_849934.1, NM_179603.2
UniGeneiAt.21686

Genome annotation databases

EnsemblPlantsiAT2G04350.1; AT2G04350.1; AT2G04350
AT2G04350.2; AT2G04350.2; AT2G04350
GeneIDi814974
GrameneiAT2G04350.1; AT2G04350.1; AT2G04350
AT2G04350.2; AT2G04350.2; AT2G04350
KEGGiath:AT2G04350

Similar proteinsi

Entry informationi

Entry nameiLACS8_ARATH
AccessioniPrimary (citable) accession number: Q9SJD4
Secondary accession number(s): Q940V0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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