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Q9SJD4

- LACS8_ARATH

UniProt

Q9SJD4 - LACS8_ARATH

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Protein

Long chain acyl-CoA synthetase 8

Gene
LACS8, At2g04350, T1O3, T23O15.3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate and linoleate.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

Cofactori

Magnesium By similarity.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi279 – 29012ATP Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: UniProtKB

GO - Biological processi

  1. fatty acid metabolic process Source: UniProtKB
  2. long-chain fatty acid metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G04350-MONOMER.
ARA:GQT-1997-MONOMER.
MetaCyc:AT2G04350-MONOMER.
SABIO-RKQ9SJD4.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Long chain acyl-CoA synthetase 8 (EC:6.2.1.3)
Gene namesi
Name:LACS8
Ordered Locus Names:At2g04350
ORF Names:T1O3, T23O15.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G04350.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast envelope Source: TAIR
  2. endoplasmic reticulum Source: TAIR
  3. Golgi apparatus Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Long chain acyl-CoA synthetase 8PRO_0000401417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9SJD4.
PRIDEiQ9SJD4.

Expressioni

Gene expression databases

GenevestigatoriQ9SJD4.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G04350.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SJD4.
SMRiQ9SJD4. Positions 116-711.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni554 – 58229Fatty acid-binding Reviewed predictionAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1022.
HOGENOMiHOG000159459.
InParanoidiQ9SJD4.
KOiK01897.
OMAiNRACNFA.
PhylomeDBiQ9SJD4.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SJD4-1 [UniParc]FASTAAdd to Basket

« Hide

MEDSGVNPMD SPSKGSDFGV YGIIGGGIVA LLVPVLLSVV LNGTKKGKKR    50
GVPIKVGGEE GYTMRHARAP ELVDVPWEGA ATMPALFEQS CKKYSKDRLL 100
GTREFIDKEF ITASDGRKFE KLHLGEYKWQ SYGEVFERVC NFASGLVNVG 150
HNVDDRVAIF SDTRAEWFIA FQGCFRQSIT VVTIYASLGE EALIYSLNET 200
RVSTLICDSK QLKKLSAIQS SLKTVKNIIY IEEDGVDVAS SDVNSMGDIT 250
VSSISEVEKL GQKNAVQPIL PSKNGVAVIM FTSGSTGLPK GVMITHGNLV 300
ATAAGVMKVV PKLDKNDTYI AYLPLAHVFE LEAEIVVFTS GSAIGYGSAM 350
TLTDTSNKVK KGTKGDVSAL KPTIMTAVPA ILDRVREGVL KKVEEKGGMA 400
KTLFDFAYKR RLAAVDGSWF GAWGLEKMLW DALVFKKIRA VLGGHIRFML 450
VGGAPLSPDS QRFINICMGS PIGQGYGLTE TCAGATFSEW DDPAVGRVGP 500
PLPCGYVKLV SWEEGGYRIS DKPMPRGEIV VGGNSVTAGY FNNQEKTDEV 550
YKVDEKGTRW FYTGDIGRFH PDGCLEVIDR KKDIVKLQHG EYVSLGKVEA 600
ALGSSNYVDN IMVHADPINS YCVALVVPSR GALEKWAEEA GVKHSEFAEL 650
CEKGEAVKEV QQSLTKAGKA AKLEKFELPA KIKLLSEPWT PESGLVTAAL 700
KIKREQIKSK FKDELSKLYA 720
Length:720
Mass (Da):78,343
Last modified:May 1, 2000 - v1
Checksum:i96ED3FD23AD2ED75
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti256 – 2638EVEKLGQK → DFFKLPPH in AAK96568. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF503758 mRNA. Translation: AAM28875.1.
AC006951 Genomic DNA. Translation: AAD25843.1.
AC007213 Genomic DNA. Translation: AAM15458.1.
CP002685 Genomic DNA. Translation: AEC05822.1.
CP002685 Genomic DNA. Translation: AEC05823.1.
AY052664 mRNA. Translation: AAK96568.1.
BT002288 mRNA. Translation: AAN72299.1.
PIRiE84456.
RefSeqiNP_178516.1. NM_126468.3.
NP_849934.1. NM_179603.2.
UniGeneiAt.21686.

Genome annotation databases

EnsemblPlantsiAT2G04350.1; AT2G04350.1; AT2G04350.
AT2G04350.2; AT2G04350.2; AT2G04350.
GeneIDi814974.
KEGGiath:AT2G04350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF503758 mRNA. Translation: AAM28875.1 .
AC006951 Genomic DNA. Translation: AAD25843.1 .
AC007213 Genomic DNA. Translation: AAM15458.1 .
CP002685 Genomic DNA. Translation: AEC05822.1 .
CP002685 Genomic DNA. Translation: AEC05823.1 .
AY052664 mRNA. Translation: AAK96568.1 .
BT002288 mRNA. Translation: AAN72299.1 .
PIRi E84456.
RefSeqi NP_178516.1. NM_126468.3.
NP_849934.1. NM_179603.2.
UniGenei At.21686.

3D structure databases

ProteinModelPortali Q9SJD4.
SMRi Q9SJD4. Positions 116-711.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G04350.1-P.

Proteomic databases

PaxDbi Q9SJD4.
PRIDEi Q9SJD4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G04350.1 ; AT2G04350.1 ; AT2G04350 .
AT2G04350.2 ; AT2G04350.2 ; AT2G04350 .
GeneIDi 814974.
KEGGi ath:AT2G04350.

Organism-specific databases

TAIRi AT2G04350.

Phylogenomic databases

eggNOGi COG1022.
HOGENOMi HOG000159459.
InParanoidi Q9SJD4.
KOi K01897.
OMAi NRACNFA.
PhylomeDBi Q9SJD4.

Enzyme and pathway databases

UniPathwayi UPA00199 .
BioCyci ARA:AT2G04350-MONOMER.
ARA:GQT-1997-MONOMER.
MetaCyc:AT2G04350-MONOMER.
SABIO-RK Q9SJD4.

Gene expression databases

Genevestigatori Q9SJD4.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
    Shockey J.M., Fulda M.S., Browse J.A.
    Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
    Shockey J.M., Fulda M.S., Browse J.
    Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION.
  6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLACS8_ARATH
AccessioniPrimary (citable) accession number: Q9SJD4
Secondary accession number(s): Q940V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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