Q9SJC4 (NUDT6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nudix hydrolase 6
Short name=AtNUDT6
EC=3.6.1.-

Alternative name(s):
ADP-ribose pyrophosphatase
EC=3.6.1.13
NADH pyrophosphatase
EC=3.6.1.22

Gene names

Name:	NUDT6
Synonyms:	NUDX6
Ordered Locus Names:	At2g04450
ORF Names:	T1O3.14

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably mediates the hydrolysis of some nucleoside diphosphate derivatives. In vitro, it can use both NADH and ADP-ribose as substrates; however the relevance of such substrates in vivo is unclear. Ref.4
Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate. NAD+ + H2O = AMP + NMN.
Cofactor	Magnesium or manganese By similarity.
Tissue specificity	Expressed in stems and leaves. Weakly or not expressed in roots. Ref.4
Sequence similarities	Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain.
Biophysicochemical properties	Kinetic parameters: KM=23.0 µM for ADP-ribose Ref.4 KM=13.7 µM for NADH Vmax=0.18 µmol/min/mg enzyme with ADP-ribose as substrate Vmax=0.32 µmol/min/mg enzyme with NADPH as substrate

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of salicylic acid mediated signaling pathway Inferred from expression pattern. Source: TAIR response to other organism Inferred from expression pattern. Source: TAIR
Molecular function	ADP-ribose diphosphatase activity Inferred from direct assay Ref.4. Source: TAIR NAD binding Inferred from direct assay Ref.4. Source: TAIR NAD+ diphosphatase activity Inferred from electronic annotation. Source: EC NADH pyrophosphatase activity Inferred from direct assay. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Nudix hydrolase 6		PRO_0000057126
Regions
	Domain	101 – 233	133	Nudix hydrolase
	Motif	139 – 160	22	Nudix box
Sites
	Metal binding	154	1	Magnesium or manganese By similarity
	Metal binding	158	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SJC4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B8140657498E57B4	FASTA	283	31,913
	10 20 30 40 50 60 MDNEDQESLL LQGVPDNYGG VKVNLTEPMT IEDFVPKLRA SLVYWSNQGT KGIWLKLADG 70 80 90 100 110 120 LDNLIAPAKA EGFVCHHAER EYTMLTSWIA DVPSTLPANA SHRIGVGAFV LNKKTKEVLV 130 140 150 160 170 180 VQEIDGHFKG TGVWKLPTGV VKEGENIWEG ALREVEEETG IKTKFVEVLA FRESHQAFLE 190 200 210 220 230 240 IKTDIFFLCE LEPTTFEIKK QDSEILAAKW MPIEEYVNQP WNQKKELFRF MANICLKRLQ 250 260 270 280 EMEYMGFSKV LTTTSSGKES YLYCNTDHAN LLNATRGLAS TSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Identification of Arabidopsis genes differentially expressed in response to virulent and avirulent strains of Pseudomonas syringae and salicylic acid." Gomez-Buitrago A.M., Lindsrtom L., Raina R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 136-283.
[4]	"Comprehensive analysis of cytosolic nudix hydrolases in Arabidopsis thaliana." Ogawa T., Ueda Y., Yoshimura K., Shigeoka S. J. Biol. Chem. 280:25277-25283(2005) [PubMed: 15878881] [Abstract] Cited for: FUNCTION IN VITRO, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006951 Genomic DNA. Translation: AAD25833.1. CP002685 Genomic DNA. Translation: AEC05837.1. CB185816 mRNA. No translation available.
IPI	IPI00527960.
PIR	G84457.
RefSeq	NP_178526.1. NM_126478.3.
UniGene	At.41308.
3D structure databases
ProteinModelPortal	Q9SJC4.
SMR	Q9SJC4. Positions 8-91, 103-265.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SJC4.
Proteomic databases
PRIDE	Q9SJC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G04450.1; AT2G04450.1; AT2G04450.
GeneID	814985.
GenomeReviews	Gene locus AT2G04450 in contig CT485783_GR.
KEGG	ath:AT2G04450.
NMPDR	fig|3702.1.peg.8029.
Organism-specific databases
TAIR	At2g04450.
Phylogenomic databases
eggNOG	KOG0648.
GeneTree	EPGT00050000010426.
HOGENOM	HBG593794.
InParanoid	Q9SJC4.
OMA	FMANICL.
PhylomeDB	Q9SJC4.
ProtClustDB	CLSN2683982.
Gene expression databases
ArrayExpress	Q9SJC4.
Genevestigator	Q9SJC4.
GermOnline	AT2G04450. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003293. Nudix_hydrolase6-like. IPR020084. NUDIX_hydrolase_CS. IPR000086. NUDIX_hydrolase_dom. IPR015797. NUDIX_hydrolase_dom-like. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR01356. GFGPROTEIN.
SUPFAM	SSF55811. NUDIX_hydrolase. 1 hit.
PROSITE	PS51462. NUDIX. 1 hit. PS00893. NUDIX_BOX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUDT6_ARATH

Accession

Primary (citable) accession number: Q9SJC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	May 1, 2000
Last modified:	September 21, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program