Reviewed,
UniProtKB/Swiss-Prot Q9SJC4 (NUDT6_ARATH)
Last modified
July 7, 2009.
Version 53.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Nudix hydrolase 6 Short name=AtNUDT6 EC=3.6.1.- Alternative name(s): ADP-ribose pyrophosphatase EC=3.6.1.13 NADH pyrophosphatase EC=3.6.1.22 | ||||||||
| Gene names |
| ||||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 283 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Probably mediates the hydrolysis of some nucleoside diphosphate derivatives. In vitro, it can use both NADH and ADP-ribose as substrates; however the relevance of such substrates in vivo is unclear. Ref.3 |
| Catalytic activity | ADP-ribose + H2O = AMP + D-ribose 5-phosphate. NAD+ + H2O = AMP + NMN. |
| Cofactor | Magnesium or manganese By similarity. |
| Tissue specificity | Expressed in stems and leaves. Weakly or not expressed in roots. Ref.3 |
| Sequence similarities | Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain. |
| Biophysicochemical properties | Kinetic parameters: KM=23.0 µM for ADP-ribose KM=13.7 µM for NADH Vmax=0.18 µmol/min/mg enzyme with ADP-ribose as substrate Vmax=0.32 µmol/min/mg enzyme with NADPH as substrate |
Ontologies
| Keywords | |
|---|---|
| Ligand | Magnesium Manganese Metal-binding NAD |
| Molecular function | Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | response to other organism Inferred from expression pattern. Source: TAIR |
| Molecular function | ADP-ribose diphosphatase activity Ref.3 Inferred from direct assay. Source: TAIR NAD or NADH binding Ref.3Inferred from direct assay. Source: TAIR NAD+ diphosphatase activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 283 | 283 | Nudix hydrolase 6 | PRO_0000057126 | |||||
Regions | |||||||||
| Domain | 102 – 237 | 136 | Nudix hydrolase | ||||||
| Motif | 139 – 160 | 22 | Nudix box | ||||||
Sites | |||||||||
| Metal binding | 154 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 158 | 1 | Magnesium or manganese By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C.Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Identification of Arabidopsis genes differentially expressed in response to virulent and avirulent strains of Pseudomonas syringae and salicylic acid." Gomez-Buitrago A.M., Lindsrtom L., Raina R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 136-283. |
| [3] | "Comprehensive analysis of cytosolic nudix hydrolases in Arabidopsis thaliana." Ogawa T., Ueda Y., Yoshimura K., Shigeoka S. J. Biol. Chem. 280:25277-25283(2005) [PubMed: 15878881] [Abstract] Cited for: FUNCTION IN VITRO, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY. |
Cross-references
Sequence databases | |
|---|---|
| AC006951 Genomic DNA. Translation: AAD25833.1. CB185816 mRNA. No translation available. | |
| IPI | IPI00527960. |
| PIR | G84457. |
| RefSeq | NP_178526.1. |
| UniGene | At.41308 |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 814985. |
| GenomeReviews | Gene locus AT2G04450 in contig CT485783_GR. |
| KEGG | ath:AT2G04450. |
| NMPDR | fig|3702.1.peg.8029. |
Organism-specific databases | |
| TAIR | At2g04450. |
Phylogenomic databases | |
| OMA | Q9SJC4. LAFRESH. |
Enzyme and pathway databases | |
| BRENDA | 3.6.1.13. 302. 3.6.1.22. 302. |
Gene expression databases | |
| ArrayExpress | Q9SJC4. |
| GermOnline | AT2G04450. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR003293. GFG_antis. IPR000086. NUDIX_hydrolase_core. [Graphical view] |
| Gene3D | G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit. |
| Pfam | PF00293. NUDIX. 1 hit. [Graphical view] |
| PRINTS | PR01356. GFGPROTEIN. |
| PROSITE | PS00893. NUDIX. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NUDT6_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9SJC4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


