Reviewed, UniProtKB/Swiss-Prot Q9SJC4 (NUDT6_ARATH)

Names and origin

Protein names

Recommended name:
    Nudix hydrolase 6
      Short name=AtNUDT6
    EC=3.6.1.-
Alternative name(s):
    ADP-ribose pyrophosphatase
    EC=3.6.1.13
    NADH pyrophosphatase
    EC=3.6.1.22

Gene names

Name:	NUDT6
Synonyms:	NUDX6
Ordered Locus Names:	At2g04450
ORF Names:	T1O3.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably mediates the hydrolysis of some nucleoside diphosphate derivatives. In vitro, it can use both NADH and ADP-ribose as substrates; however the relevance of such substrates in vivo is unclear. Ref.3
Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate. NAD+ + H2O = AMP + NMN.
Cofactor	Magnesium or manganese By similarity.
Tissue specificity	Expressed in stems and leaves. Weakly or not expressed in roots. Ref.3
Sequence similarities	Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain.
Biophysicochemical properties	Kinetic parameters: KM=23.0 µM for ADP-ribose KM=13.7 µM for NADH Vmax=0.18 µmol/min/mg enzyme with ADP-ribose as substrate Vmax=0.32 µmol/min/mg enzyme with NADPH as substrate

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to other organism Inferred from expression pattern. Source: TAIR
Molecular function	ADP-ribose diphosphatase activity Ref.3 Inferred from direct assay. Source: TAIR NAD or NADH binding Ref.3 Inferred from direct assay. Source: TAIR NAD+ diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Nudix hydrolase 6		PRO_0000057126
Regions
	Domain	102 – 237	136	Nudix hydrolase
	Motif	139 – 160	22	Nudix box
Sites
	Metal binding	154	1	Magnesium or manganese By similarity
	Metal binding	158	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SJC4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B8140657498E57B4	FASTA	283	31,913
	10 20 30 40 50 60 MDNEDQESLL LQGVPDNYGG VKVNLTEPMT IEDFVPKLRA SLVYWSNQGT KGIWLKLADG 70 80 90 100 110 120 LDNLIAPAKA EGFVCHHAER EYTMLTSWIA DVPSTLPANA SHRIGVGAFV LNKKTKEVLV 130 140 150 160 170 180 VQEIDGHFKG TGVWKLPTGV VKEGENIWEG ALREVEEETG IKTKFVEVLA FRESHQAFLE 190 200 210 220 230 240 IKTDIFFLCE LEPTTFEIKK QDSEILAAKW MPIEEYVNQP WNQKKELFRF MANICLKRLQ 250 260 270 280 EMEYMGFSKV LTTTSSGKES YLYCNTDHAN LLNATRGLAS TSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Identification of Arabidopsis genes differentially expressed in response to virulent and avirulent strains of Pseudomonas syringae and salicylic acid." Gomez-Buitrago A.M., Lindsrtom L., Raina R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 136-283.
[3]	"Comprehensive analysis of cytosolic nudix hydrolases in Arabidopsis thaliana." Ogawa T., Ueda Y., Yoshimura K., Shigeoka S. J. Biol. Chem. 280:25277-25283(2005) [PubMed: 15878881] [Abstract] Cited for: FUNCTION IN VITRO, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC006951 Genomic DNA. Translation: AAD25833.1. CB185816 mRNA. No translation available.
IPI	IPI00527960.
PIR	G84457.
RefSeq	NP_178526.1.
UniGene	At.41308
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	814985.
GenomeReviews	Gene locus AT2G04450 in contig CT485783_GR.
KEGG	ath:AT2G04450.
NMPDR	fig|3702.1.peg.8029.
Organism-specific databases
TAIR	At2g04450.
Phylogenomic databases
OMA	Q9SJC4. LAFRESH.
Enzyme and pathway databases
BRENDA	3.6.1.13. 302. 3.6.1.22. 302.
Gene expression databases
ArrayExpress	Q9SJC4.
GermOnline	AT2G04450. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003293. GFG_antis. IPR000086. NUDIX_hydrolase_core. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR01356. GFGPROTEIN.
PROSITE	PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUDT6_ARATH

Accession

Primary (citable) accession number: Q9SJC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	May 1, 2000
Last modified:	July 7, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)