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Protein

ATPase 5, plasma membrane-type

Gene

AHA5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses (By similarity).By similarity

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3294-aspartylphosphate intermediateBy similarity1
Metal bindingi588MagnesiumBy similarity1
Metal bindingi592MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processHydrogen ion transport, Ion transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G24520-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase 5, plasma membrane-type (EC:3.6.3.6)
Alternative name(s):
Proton pump 5
Gene namesi
Name:AHA5
Ordered Locus Names:At2g24520
ORF Names:F25P17.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G24520.
TAIRilocus:2046623. AT2G24520.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 61CytoplasmicSequence analysisAdd BLAST60
Transmembranei62 – 81Helical; Name=1Sequence analysisAdd BLAST20
Topological domaini82 – 93ExtracellularSequence analysisAdd BLAST12
Transmembranei94 – 114Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini115 – 243CytoplasmicSequence analysisAdd BLAST129
Transmembranei244 – 264Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini265 – 273ExtracellularSequence analysis9
Transmembranei274 – 291Helical; Name=4Sequence analysisAdd BLAST18
Topological domaini292 – 643CytoplasmicSequence analysisAdd BLAST352
Transmembranei644 – 665Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini666 – 670ExtracellularSequence analysis5
Transmembranei671 – 693Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini694 – 709CytoplasmicSequence analysisAdd BLAST16
Transmembranei710 – 730Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini731 – 751ExtracellularSequence analysisAdd BLAST21
Transmembranei752 – 772Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini773 – 784CytoplasmicSequence analysisAdd BLAST12
Transmembranei785 – 805Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini806 – 813ExtracellularSequence analysis8
Transmembranei814 – 834Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini835 – 949CytoplasmicSequence analysisAdd BLAST115

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000462782 – 949ATPase 5, plasma membrane-typeAdd BLAST948

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei881PhosphothreonineBy similarity1
Modified residuei899PhosphoserineBy similarity1
Modified residuei931PhosphoserineBy similarity1
Modified residuei948PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9SJB3.
PRIDEiQ9SJB3.

PTM databases

iPTMnetiQ9SJB3.

Expressioni

Gene expression databases

GenevisibleiQ9SJB3. AT.

Interactioni

Subunit structurei

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H+-ATPase (By similarity).By similarity

Protein-protein interaction databases

STRINGi3702.AT2G24520.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SJB3.
SMRiQ9SJB3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni947 – 949Interaction with 14-3-3 proteinsBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiQ9SJB3.
KOiK01535.
OrthoDBiEOG093602AD.
PhylomeDBiQ9SJB3.

Family and domain databases

CDDicd02076. P-type_ATPase_H. 1 hit.
Gene3Di3.40.1110.10. 1 hit.
InterProiView protein in InterPro
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
PfamiView protein in Pfam
PF00690. Cation_ATPase_N. 1 hit.
PRINTSiPR00120. HATPASE.
SMARTiView protein in SMART
SM00831. Cation_ATPase_N. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81653. SSF81653. 1 hit.
SSF81665. SSF81665. 3 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiView protein in PROSITE
PS00154. ATPASE_E1_E2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SJB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELDHIKNE SVDLVRIPME EVFEELKCTK QGLTANEASH RLDVFGPNKL
60 70 80 90 100
EEKKESKLLK FLGFMWNPLS WVMEVAALMA IALANGGGRP PDWQDFVGIV
110 120 130 140 150
CLLLINSTIS FIEENNAGNA AAALMAGLAP KTKVLRDNQW SEQEASILVP
160 170 180 190 200
GDVISIKLGD IIPADARLLD GDPLKIDQSS LTGESIPVTK NPSDEVFSGS
210 220 230 240 250
ICKQGEIEAI VIATGVHTFF GKAAHLVDNT NQIGHFQKVL TSIGNFCICS
260 270 280 290 300
IALGIIVELL VMYPIQRRRY RDGIDNLLVL LIGGIPIAMP SVLSVTMATG
310 320 330 340 350
SHRLFQQGAI TKRMTAIEEM AGMDVLCCDK TGTLTLNKLT VDKNLVEVFA
360 370 380 390 400
KGVGKEHVFL LAARASRIEN QDAIDAAIVG MLADPKEARA GVREVHFFPF
410 420 430 440 450
NPVDKRTALT YVDSDGNWHR ASKGAPEQIL NLCNCKEDVR RKVHGVIDKF
460 470 480 490 500
AERGLRSLAV ARQEVLEKKK DAPGGPWQLV GLLPLFDPPR HDSAETIRRA
510 520 530 540 550
LNLGVNVKMI TGDQLAIGKE TGRRLGMGTN MYPSSALLGQ VKDSSLGALP
560 570 580 590 600
VDELIEKADG FAGVFPEHKY EIVHRLQQRN HICGMTGDGV NDAPALKKAD
610 620 630 640 650
IGIAVVDATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV
660 670 680 690 700
SITIRIVFGF MFIALIWQFD FSPFMVLIIA ILNDGTIMTI SKDRMKPSPQ
710 720 730 740 750
PDSWKLRDIF STGVVLGGYQ ALMTVVFFWV MKDSDFFSNY FGVRPLSQRP
760 770 780 790 800
EQMMAALYLQ VSIISQALIF VTRSRSWSYA ECPGLLLLGA FVIAQLVATF
810 820 830 840 850
IAVYANWSFA RIEGAGWGWA GVIWLYSFLT YIPLDLLKFG IRYVLSGKAW
860 870 880 890 900
LNLLENKTAF TTKKDYGKEE REAQWAAAQR TLHGLQPAEK NNIFNEKNSY
910 920 930 940
SELSQIAEQA KRRAEVVRLR EINTLKGHVE SVVKLKGLDI DTIQQHYTV
Length:949
Mass (Da):104,739
Last modified:January 23, 2007 - v3
Checksum:i2D042497CD564C10
GO

Sequence cautioni

The sequence AAD23893 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AEC07588 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → G in AAD23893 (PubMed:10617197).Curated1
Sequence conflicti1M → G in AEC07588 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006954 Genomic DNA. Translation: AAD23893.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07588.1. Sequence problems.
PIRiF84637.
RefSeqiNP_001318282.1. NM_001335933.1.
NP_180028.1. NM_128013.2.
UniGeneiAt.52895.

Genome annotation databases

GeneIDi816988.
KEGGiath:AT2G24520.

Similar proteinsi

Entry informationi

Entry nameiPMA5_ARATH
AccessioniPrimary (citable) accession number: Q9SJB3
Secondary accession number(s): F4IPQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families