Q9SJA1 (UBP19_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 19
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 19
Short name=AtUBP19
Ubiquitin thioesterase 19
Ubiquitin-specific-processing protease 19

Gene names

Name:	UBP19
Ordered Locus Names:	At2g24640
ORF Names:	F25P17.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	672 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 MYND-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SJA1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 672	672	Ubiquitin carboxyl-terminal hydrolase 19		PRO_0000313045
Regions
	Transmembrane	11 – 31	21	Helical; Potential
	Zinc finger	64 – 101	38	MYND-type
Sites
	Active site	183	1	Nucleophile By similarity
	Active site	439	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 0A3B1BDA29F3FB30	FASTA	672	75,720
	10 20 30 40 50 60 MHEVGLFVDL NSFTQLILTL FFVSIGLLYF VKRTAAKYFE VGGGSGGFDR DHRRDFMVSD 70 80 90 100 110 120 TAECSVCGKA TTKKCSRCKS VRYCSAACQT SDWKSGHKLK CKGFRSTDSS PVRRDDIDFE 130 140 150 160 170 180 ASLFGNRSAS KKTRIALVPQ QSQSKATLKP TDVLFPYESF VRYYNWDRPI MAPCGLTNCG 190 200 210 220 230 240 NSCFANVVLQ CLSWTRPLVA YLLERGHKRE CRRNDWCFLC EFENHLDRAN YSRFPFSPMN 250 260 270 280 290 300 IISRLPNIGG NLGYGRQEDA HELMRFAIDM MQSVCLDEFG GEKVVPPRAQ ETTLIQYIFG 310 320 330 340 350 360 GLLQSQVQCT ACSNVSDQYE NMMDLTVEIH GDAVSLEECL DQFTAKEWLQ GDNLYKCDRC 370 380 390 400 410 420 DDYVKACKRL SIRCAPNILT IALKRFQGGR FGKLNKRISF PETFDLGPYM SGGGEGSDVY 430 440 450 460 470 480 KLYAVIVHLD MLNASFFGHY ICYVKDFRGN WYRIDDSEVE KVELEDVLSQ RAYMLLYSRV 490 500 510 520 530 540 QPRPSNLRSE ESQDEKKTDT LNTESNQDGS VESSGVGTND TSVSSLCNGI ISHSEDPEYE 550 560 570 580 590 600 KESSLSASVP VSEEGKEVDV KVDTVDSESN RSIDMEHDSG TDHQEEEANG KEDPTVENLA 610 620 630 640 650 660 VDSSCLDITT PSPSAATEFI PQENERSDTE SKPLEKEHSD TESNKPLEKE HLDSESKPLE 670 KEHSDTEMID AQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006954 Genomic DNA. Translation: AAD23896.2. CP002685 Genomic DNA. Translation: AEC07607.1. AF360315 mRNA. Translation: AAK26025.1. AY056366 mRNA. Translation: AAL07252.1.
IPI	IPI00518677.
PIR	B84639.
RefSeq	NP_565576.1. NM_128025.3.
UniGene	At.14198.
3D structure databases
ProteinModelPortal	Q9SJA1.
SMR	Q9SJA1. Positions 63-104, 175-478.
ModBase	Search...
Protein family/group databases
MEROPS	C19.A09.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G24640.1; AT2G24640.1; AT2G24640.
GeneID	817000.
KEGG	ath:AT2G24640.
Organism-specific databases
TAIR	At2g24640.
Phylogenomic databases
eggNOG	COG5533.
HOGENOM	HOG000097839.
InParanoid	Q9SJA1.
KO	K11855.
OMA	PLEKEHS.
PhylomeDB	Q9SJA1.
ProtClustDB	CLSN2685876.
Gene expression databases
ArrayExpress	Q9SJA1.
Genevestigator	Q9SJA1.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR002893. Znf_MYND. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF01753. zf-MYND. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. False negative. PS50235. UCH_2_3. 1 hit. PS01360. ZF_MYND_1. 1 hit. PS50865. ZF_MYND_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP19_ARATH

Accession

Primary (citable) accession number: Q9SJA1
Secondary accession number(s): Q9C5D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program