Shikimate kinase 1, chloroplastic precursor - Arabidopsis thaliana (Mouse-ear cress)

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Q9SJ05 (SK1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 79. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Shikimate kinase 1, chloroplastic
Short name=AtSK1
EC=2.7.1.71

Gene names

Name:	SK1
Ordered Locus Names:	At2g21940
ORF Names:	F7D8.26

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. Ref.5 Ref.6
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. Ref.6
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.
Subunit structure	Homodimer. Ref.6
Subcellular location	Plastid › chloroplast By similarity.
Induction	By heat stress. Ref.6
Disruption phenotype	No visible phenotype under normal growth conditions. Ref.5
Miscellaneous	SK1 forms a homodimer in solution, which may facilitate its relative thermostability when exposed at 37 degrees Celsius (Ref.6).
Sequence similarities	Belongs to the shikimate kinase family.
Biophysicochemical properties	Kinetic parameters: K_M=648 µM for shikimate Ref.5 K_M=218 µM for ATP

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Stress response
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW shikimate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SJ05-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 66

Chloroplast Potential

Chain

67 – 303

237

Shikimate kinase 1, chloroplastic

PRO_0000002292

Regions

Nucleotide binding

109 – 116

ATP By similarity

Sites

Metal binding

116

Magnesium By similarity

Binding site

134

Substrate By similarity

Binding site

159

Substrate By similarity

Binding site

181

Substrate; via amide nitrogen By similarity

Binding site

220

ATP By similarity

Amino acid modifications

Disulfide bond

Interchain Potential

Experimental info

Sequence conflict

229

D → N in BAC43483. Ref.3

Sequence conflict

269

N → D in ACD85800. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified October 14, 2008. Version 2.
Checksum: B3717FD63F57397D
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FASTA

303

33,982

        10         20         30         40         50         60 
MEAAITQRIQ YPSWVDCRKV ECKPQRGSLR YSQQVKVDRR FRGLSLARLQ PERRNDQRRA 

        70         80         90        100        110        120 
VSPAVSCSDN NSSALLETGS VYPFDEDILK RKAEEVKPYL NGRSMYLVGM MGSGKTTVGK 

       130        140        150        160        170        180 
LMSKVLGYTF FDCDTLIEQA MNGTSVAEIF VHHGENFFRG KETDALKKLS SRYQVVVSTG 

       190        200        210        220        230        240 
GGAVIRPINW KYMHKGISIW LDVPLEALAH RIAAVGTDSR PLLHDESGDA YSVAFKRLSA 

       250        260        270        280        290        300 
IWDERGEAYT NANARVSLEN IAAKRGYKNV SDLTPTEIAI EAFEQVLSFL EKEETMEIPD 


GDL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Evolutionary diversification of plant shikimate kinase gene duplicates." Fucile G., Falconer S., Christendat D. PLoS Genet. 4:E1000292-E1000292(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY, NOMENCLATURE, DISRUPTION PHENOTYPE.
[6]	"Structural and biochemical investigation of two Arabidopsis shikimate kinases: the heat-inducible isoform is thermostable." Fucile G., Garcia C., Carlsson J., Sunnerhagen M., Christendat D. Protein Sci. 20:1125-1136(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007019 Genomic DNA. Translation: AAD20411.1. CP002685 Genomic DNA. Translation: AEC07240.1. CP002685 Genomic DNA. Translation: AEC07241.1. CP002685 Genomic DNA. Translation: AEC07244.1. AK118899 mRNA. Translation: BAC43483.2. BT032871 mRNA. Translation: ACD85800.1.
IPI	IPI00524992. IPI00914311.
PIR	A84607.
RefSeq	NP_001077938.1. NM_001084469.1. NP_179785.2. NM_127763.4. NP_973507.2. NM_201778.2.
UniGene	At.47529. At.50089.
3D structure databases
ProteinModelPortal	Q9SJ05.
SMR	Q9SJ05. Positions 86-293.
ModBase	Search...
Proteomic databases
PaxDb	Q9SJ05.
PRIDE	Q9SJ05.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G21940.1; AT2G21940.1; AT2G21940. AT2G21940.2; AT2G21940.2; AT2G21940. AT2G21940.5; AT2G21940.5; AT2G21940.
GeneID	816730.
KEGG	ath:AT2G21940.
Organism-specific databases
TAIR	At2g21940.
Phylogenomic databases
eggNOG	COG0703.
HOGENOM	HOG000032570.
InParanoid	Q9SJ05.
KO	K00891.
PhylomeDB	Q9SJ05.
ProtClustDB	PLN02199.
Enzyme and pathway databases
BioCyc	ARA:AT2G21940-MONOMER. MetaCyc:AT2G21940-MONOMER.
UniPathway	UPA00053; UER00088.
Gene expression databases
ArrayExpress	Q9SJ05.
Genevestigator	Q9SJ05.
Family and domain databases
InterPro	IPR000623. Shikimate_kinase. IPR023000. Shikimate_kinase_CS. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SK1_ARATH

Accession

Primary (citable) accession number: Q9SJ05
Secondary accession number(s): B3DN83, Q3EBW8, Q8GT76

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 14, 2008
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents