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Protein

Laccase-5

Gene

LAC5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Lignin degradation and detoxification of lignin-derived products.By similarity

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Copper 1; type 2By similarity
Metal bindingi86 – 861Copper 2; type 3By similarity
Metal bindingi129 – 1291Copper 2; type 3By similarity
Metal bindingi131 – 1311Copper 3; type 3By similarity
Metal bindingi481 – 4811Copper 4; type 1By similarity
Metal bindingi484 – 4841Copper 1; type 2By similarity
Metal bindingi486 – 4861Copper 3; type 3By similarity
Metal bindingi543 – 5431Copper 3; type 3By similarity
Metal bindingi544 – 5441Copper 4; type 1By similarity
Metal bindingi545 – 5451Copper 2; type 3By similarity
Metal bindingi549 – 5491Copper 4; type 1By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB-KW
  2. response to copper ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G40370-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-5 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 5
Diphenol oxidase 5
Urishiol oxidase 5
Gene namesi
Name:LAC5
Synonyms:LAC1
Ordered Locus Names:At2g40370
ORF Names:T3G21.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G40370.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 580555Laccase-5PRO_0000283633Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi392 – 3921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi410 – 4101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi443 – 4431N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SIY8.
PRIDEiQ9SIY8.

Expressioni

Tissue specificityi

Ubiquitous and constitutive.2 Publications

Gene expression databases

GenevestigatoriQ9SIY8.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G40370.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SIY8.
SMRiQ9SIY8. Positions 46-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 150117Plastocyanin-like 1Add
BLAST
Domaini160 – 312153Plastocyanin-like 2Add
BLAST
Domaini428 – 564137Plastocyanin-like 3Add
BLAST

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2132.
HOGENOMiHOG000241916.
InParanoidiQ9SIY8.
OMAiCETHNSI.
PhylomeDBiQ9SIY8.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 4 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SIY8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDVTKSLLCF ISFVAFLLFS SVAEANKAHH HEFIIQATKV KRLCETHNSI
60 70 80 90 100
TVNGMFPGPM LVVNNGDTLV VKVINRARYN ITIHWHGVRQ MRTGWADGPE
110 120 130 140 150
FVTQCPIRPG SSYTYRFTIQ GQEGTLWWHA HSSWLRATVY GSLLVFPPAG
160 170 180 190 200
SSYPFTKPHR NVPLLLGEWW DANPVDVLRE SIRTGGAPNN SDAYTINGQP
210 220 230 240 250
GDLYKCSSQD TTVVPINVGE TILLRVINSA LNQPLFFTVA NHKLTVVGAD
260 270 280 290 300
ASYLKPFTTN VIVLGPGQTT DVLITGDQPP NRYYMAARAY QSAQNAPFGN
310 320 330 340 350
TTTTAILQYK SAPCCGVGGG SGTKKGNSFK PIMPILPAYN DTNTVTRFSQ
360 370 380 390 400
SFRSLRRAEV PTEIDENLFV TIGLGLNNCP KNFRSRRCQG PNGTRFTASM
410 420 430 440 450
NNVSFALPSN YSLLQAHHHG IPGVFTTDFP AKPPVKFDYT GNNISRSLYQ
460 470 480 490 500
PDRGTKLYKL KYGSRVQIVL QDTGIVTPEN HPIHLHGYDF YIIAEGFGNF
510 520 530 540 550
NPKKDTAKFN LEDPPLRNTV GVPVNGWAVI RFIADNPGVW IMHCHLDAHI
560 570 580
SWGLAMAFLV ENGNGVLQTI EQPPHDLPVC
Length:580
Mass (Da):64,133
Last modified:May 1, 2000 - v1
Checksum:iEFD565D79AD887CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF506030 mRNA. Translation: AAM77221.1.
AC007020 Genomic DNA. Translation: AAD25671.1.
CP002685 Genomic DNA. Translation: AEC09819.1.
PIRiF84828.
RefSeqiNP_181568.1. NM_129597.3.
UniGeneiAt.37061.

Genome annotation databases

EnsemblPlantsiAT2G40370.1; AT2G40370.1; AT2G40370.
GeneIDi818630.
KEGGiath:AT2G40370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF506030 mRNA. Translation: AAM77221.1.
AC007020 Genomic DNA. Translation: AAD25671.1.
CP002685 Genomic DNA. Translation: AEC09819.1.
PIRiF84828.
RefSeqiNP_181568.1. NM_129597.3.
UniGeneiAt.37061.

3D structure databases

ProteinModelPortaliQ9SIY8.
SMRiQ9SIY8. Positions 46-559.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G40370.1-P.

Proteomic databases

PaxDbiQ9SIY8.
PRIDEiQ9SIY8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G40370.1; AT2G40370.1; AT2G40370.
GeneIDi818630.
KEGGiath:AT2G40370.

Organism-specific databases

TAIRiAT2G40370.

Phylogenomic databases

eggNOGiCOG2132.
HOGENOMiHOG000241916.
InParanoidiQ9SIY8.
OMAiCETHNSI.
PhylomeDBiQ9SIY8.

Enzyme and pathway databases

BioCyciARA:AT2G40370-MONOMER.

Gene expression databases

GenevestigatoriQ9SIY8.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 4 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, characterization, and expression of a cDNA clone encoding laccase from Arabidopsis thaliana."
    Gaynor J.J.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Gene structure and molecular analysis of the laccase-like multicopper oxidase (LMCO) gene family in Arabidopsis thaliana."
    McCaig B.C., Meagher R.B., Dean J.F.D.
    Planta 221:619-636(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Mutant identification and characterization of the laccase gene family in Arabidopsis."
    Cai X., Davis E.J., Ballif J., Liang M., Bushman E., Haroldsen V., Torabinejad J., Wu Y.
    J. Exp. Bot. 57:2563-2569(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiLAC5_ARATH
AccessioniPrimary (citable) accession number: Q9SIY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.