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Q9SIV9 (PPA10_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purple acid phosphatase 10
EC=3.1.3.2
Gene names
Name:PAP10
Synonyms:AT11
Ordered Locus Names:At2g16430
ORF Names:F16F14.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer; disulfide-linked By similarity.

Subcellular location

Isoform 1: Secreted By similarity Ref.1.

Isoform 2: Cytoplasm Probable Ref.1.

Tissue specificity

Expressed in roots, stems, leaves, flowers and siliques. Ref.5

Induction

By phosphate deprivation, mostly isoform 2. Ref.1

Sequence similarities

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9SIV9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9SIV9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: MGRVRKSDFG...IHHCPIRNLE → MLWFFLLQ
Note: May be due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 468443Purple acid phosphatase 10
PRO_0000372815

Regions

Region355 – 3573Substrate binding By similarity

Sites

Active site3281Proton donor By similarity
Metal binding1671Iron By similarity
Metal binding1961Iron By similarity
Metal binding1961Zinc By similarity
Metal binding1991Iron By similarity
Metal binding2331Zinc By similarity
Metal binding3181Zinc By similarity
Metal binding3551Zinc By similarity
Metal binding3571Iron By similarity
Binding site2331Substrate By similarity

Amino acid modifications

Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation1751N-linked (GlcNAc...) Potential
Glycosylation3061N-linked (GlcNAc...) Potential
Glycosylation4281N-linked (GlcNAc...) Potential
Disulfide bond377Interchain By similarity

Natural variations

Alternative sequence1 – 128128MGRVR…IRNLE → MLWFFLLQ in isoform 2.
VSP_037192

Experimental info

Sequence conflict1671D → N in AAM16283. Ref.1
Sequence conflict2801I → V in AAM16283. Ref.1
Sequence conflict4411D → G in AAM16283. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: ED1EB9821C5C118B

FASTA46854,219
        10         20         30         40         50         60 
MGRVRKSDFG SIVLVLCCVL NSLLCNGGIT SRYVRKLEAT VDMPLDSDVF RVPCGYNAPQ 

        70         80         90        100        110        120 
QVHITQGDVE GKAVIVSWVT QEAKGSNKVI YWKENSTKKH KAHGKTNTYK FYNYTSGFIH 

       130        140        150        160        170        180 
HCPIRNLEYD TKYYYVLGVG QTERKFWFFT PPEIGPDVPY TFGLIGDLGQ SYDSNITLTH 

       190        200        210        220        230        240 
YENNPTKGQA VLFVGDISYA DTYPDHDNRR WDSWGRFAER STAYQPWIWT TGNHELDFAP 

       250        260        270        280        290        300 
EIGENRPFKP FTHRYRTPYR SSGSTEPFWY SIKRGPAYII VLASYSAYGK YTPQYQWLEE 

       310        320        330        340        350        360 
EFPKVNRTET PWLIVLMHSP WYNSYDYHYM EGETMRVMYE AWFVKYKVDV VFAGHVHAYE 

       370        380        390        400        410        420 
RSERVSNIAY NVVNGICTPV KDQSAPVYIT IGDGGNIEGL ATKMTEPQPK YSAFREASFG 

       430        440        450        460 
HAIFSIKNRT HAHYGWHRNH DGYAVEGDRM WFYNRFWHPV DDSPSCNS

« Hide

Isoform 2 [UniParc].

Checksum: 84D390C33712D899
Show »

FASTA34840,909

References

« Hide 'large scale' references
[1]"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, INDUCTION, GENE FAMILY, NOMENCLATURE.
Strain: cv. Col-1.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF492662 mRNA. Translation: AAM15911.1.
AY090893 mRNA. Translation: AAM16283.1.
AC007047 Genomic DNA. Translation: AAD22297.1.
CP002685 Genomic DNA. Translation: AEC06495.1.
CP002685 Genomic DNA. Translation: AEC06496.1.
AY093236 mRNA. Translation: AAM13235.1.
BT008761 mRNA. Translation: AAP49523.1.
IPIIPI00530753.
IPI00539645.
PIRB84540.
RefSeqNP_179235.1. NM_127196.3.
NP_849960.1. NM_179629.2.
UniGeneAt.14307.

3D structure databases

HSSPHSSP built from PDB template 4KBP based on UniProtKB P80366.
ProteinModelPortalQ9SIV9.
SMRQ9SIV9. Positions 42-463.
ModBaseSearch...

Proteomic databases

PaxDbQ9SIV9.
PRIDEQ9SIV9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G16430.2; AT2G16430.2; AT2G16430.
GeneID816141.
KEGGath:AT2G16430.

Organism-specific databases

TAIRAt2g16430.

Phylogenomic databases

eggNOGCOG1409.
HOGENOMHOG000238330.
InParanoidQ9SIV9.
OMAYERTHRI.
PhylomeDBQ9SIV9.
ProtClustDBCLSN2913228.

Gene expression databases

ArrayExpressQ9SIV9.
GenevestigatorQ9SIV9.

Family and domain databases

Gene3D2.60.40.380. 1 hit.
InterProIPR004843. Metallo_PEstase_dom.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMSSF49363. Purple_Pase_N. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPA10_ARATH
AccessionPrimary (citable) accession number: Q9SIV9
Secondary accession number(s): Q8RX39
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents