Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9SIV9

- PPA10_ARATH

UniProt

Q9SIV9 - PPA10_ARATH

Protein

Purple acid phosphatase 10

Gene

PAP10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Cofactori

    Binds 1 iron ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi167 – 1671IronBy similarity
    Metal bindingi196 – 1961IronBy similarity
    Metal bindingi196 – 1961ZincBy similarity
    Metal bindingi199 – 1991IronBy similarity
    Metal bindingi233 – 2331ZincBy similarity
    Binding sitei233 – 2331SubstrateBy similarity
    Metal bindingi318 – 3181ZincBy similarity
    Active sitei328 – 3281Proton donorBy similarity
    Metal bindingi355 – 3551ZincBy similarity
    Metal bindingi357 – 3571IronBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: TAIR
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to phosphate starvation Source: TAIR
    2. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT2G16430-MONOMER.
    ARA:GQT-850-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase 10 (EC:3.1.3.2)
    Gene namesi
    Name:PAP10
    Synonyms:AT11
    Ordered Locus Names:At2g16430
    ORF Names:F16F14.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G16430.

    Subcellular locationi

    Isoform 1 : Secreted By similarity

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. cytosol Source: TAIR
    3. extracellular region Source: UniProtKB-SubCell
    4. plant-type cell wall Source: TAIR
    5. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 468443Purple acid phosphatase 10PRO_0000372815Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi377 – 377InterchainBy similarity
    Glycosylationi428 – 4281N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9SIV9.
    PRIDEiQ9SIV9.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves, flowers and siliques.1 Publication

    Inductioni

    By phosphate deprivation, mostly isoform 2.1 Publication

    Gene expression databases

    GenevestigatoriQ9SIV9.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SIV9.
    SMRiQ9SIV9. Positions 42-463.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni355 – 3573Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1409.
    HOGENOMiHOG000238330.
    InParanoidiQ9SIV9.
    OMAiYKWLEEE.
    PhylomeDBiQ9SIV9.

    Family and domain databases

    Gene3Di2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9SIV9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGRVRKSDFG SIVLVLCCVL NSLLCNGGIT SRYVRKLEAT VDMPLDSDVF    50
    RVPCGYNAPQ QVHITQGDVE GKAVIVSWVT QEAKGSNKVI YWKENSTKKH 100
    KAHGKTNTYK FYNYTSGFIH HCPIRNLEYD TKYYYVLGVG QTERKFWFFT 150
    PPEIGPDVPY TFGLIGDLGQ SYDSNITLTH YENNPTKGQA VLFVGDISYA 200
    DTYPDHDNRR WDSWGRFAER STAYQPWIWT TGNHELDFAP EIGENRPFKP 250
    FTHRYRTPYR SSGSTEPFWY SIKRGPAYII VLASYSAYGK YTPQYQWLEE 300
    EFPKVNRTET PWLIVLMHSP WYNSYDYHYM EGETMRVMYE AWFVKYKVDV 350
    VFAGHVHAYE RSERVSNIAY NVVNGICTPV KDQSAPVYIT IGDGGNIEGL 400
    ATKMTEPQPK YSAFREASFG HAIFSIKNRT HAHYGWHRNH DGYAVEGDRM 450
    WFYNRFWHPV DDSPSCNS 468
    Length:468
    Mass (Da):54,219
    Last modified:May 1, 2000 - v1
    Checksum:iED1EB9821C5C118B
    GO
    Isoform 2 (identifier: Q9SIV9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-128: MGRVRKSDFG...IHHCPIRNLE → MLWFFLLQ

    Note: May be due to intron retention. No experimental confirmation available.

    Show »
    Length:348
    Mass (Da):40,909
    Checksum:i84D390C33712D899
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti167 – 1671D → N in AAM16283. (PubMed:12021284)Curated
    Sequence conflicti280 – 2801I → V in AAM16283. (PubMed:12021284)Curated
    Sequence conflicti441 – 4411D → G in AAM16283. (PubMed:12021284)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 128128MGRVR…IRNLE → MLWFFLLQ in isoform 2. 1 PublicationVSP_037192Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492662 mRNA. Translation: AAM15911.1.
    AY090893 mRNA. Translation: AAM16283.1.
    AC007047 Genomic DNA. Translation: AAD22297.1.
    CP002685 Genomic DNA. Translation: AEC06495.1.
    CP002685 Genomic DNA. Translation: AEC06496.1.
    AY093236 mRNA. Translation: AAM13235.1.
    BT008761 mRNA. Translation: AAP49523.1.
    PIRiB84540.
    RefSeqiNP_179235.1. NM_127196.3. [Q9SIV9-1]
    NP_849960.1. NM_179629.2. [Q9SIV9-2]
    UniGeneiAt.14307.

    Genome annotation databases

    EnsemblPlantsiAT2G16430.2; AT2G16430.2; AT2G16430. [Q9SIV9-1]
    GeneIDi816141.
    KEGGiath:AT2G16430.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492662 mRNA. Translation: AAM15911.1 .
    AY090893 mRNA. Translation: AAM16283.1 .
    AC007047 Genomic DNA. Translation: AAD22297.1 .
    CP002685 Genomic DNA. Translation: AEC06495.1 .
    CP002685 Genomic DNA. Translation: AEC06496.1 .
    AY093236 mRNA. Translation: AAM13235.1 .
    BT008761 mRNA. Translation: AAP49523.1 .
    PIRi B84540.
    RefSeqi NP_179235.1. NM_127196.3. [Q9SIV9-1 ]
    NP_849960.1. NM_179629.2. [Q9SIV9-2 ]
    UniGenei At.14307.

    3D structure databases

    ProteinModelPortali Q9SIV9.
    SMRi Q9SIV9. Positions 42-463.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9SIV9.
    PRIDEi Q9SIV9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G16430.2 ; AT2G16430.2 ; AT2G16430 . [Q9SIV9-1 ]
    GeneIDi 816141.
    KEGGi ath:AT2G16430.

    Organism-specific databases

    TAIRi AT2G16430.

    Phylogenomic databases

    eggNOGi COG1409.
    HOGENOMi HOG000238330.
    InParanoidi Q9SIV9.
    OMAi YKWLEEE.
    PhylomeDBi Q9SIV9.

    Enzyme and pathway databases

    BioCyci ARA:AT2G16430-MONOMER.
    ARA:GQT-850-MONOMER.

    Gene expression databases

    Genevestigatori Q9SIV9.

    Family and domain databases

    Gene3Di 2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
      Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
      J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, INDUCTION, GENE FAMILY, NOMENCLATURE.
      Strain: cv. Col-1.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
      Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
      Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPPA10_ARATH
    AccessioniPrimary (citable) accession number: Q9SIV9
    Secondary accession number(s): Q8RX39
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3