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Protein

Purple acid phosphatase 10

Gene

PAP10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi167IronBy similarity1
Metal bindingi196IronBy similarity1
Metal bindingi196ZincBy similarity1
Metal bindingi199IronBy similarity1
Metal bindingi233ZincBy similarity1
Binding sitei233SubstrateBy similarity1
Metal bindingi318ZincBy similarity1
Active sitei328Proton donorBy similarity1
Metal bindingi355ZincBy similarity1
Metal bindingi357IronBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to phosphate starvation Source: TAIR

Keywordsi

Molecular functionHydrolase
LigandIron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G16430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 10 (EC:3.1.3.2)
Gene namesi
Name:PAP10
Synonyms:AT11
Ordered Locus Names:At2g16430
ORF Names:F16F14.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G16430.
TAIRilocus:2042689. AT2G16430.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000037281526 – 468Purple acid phosphatase 10Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi95N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi113N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi175N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi306N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi377InterchainBy similarity
Glycosylationi428N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9SIV9.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Inductioni

By phosphate deprivation, mostly isoform 2.1 Publication

Gene expression databases

ExpressionAtlasiQ9SIV9. baseline and differential.
GenevisibleiQ9SIV9. AT.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G16430.2.

Structurei

3D structure databases

ProteinModelPortaliQ9SIV9.
SMRiQ9SIV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 357Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1378. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000238330.
InParanoidiQ9SIV9.
OMAiWVTTSEP.
OrthoDBiEOG093609CL.
PhylomeDBiQ9SIV9.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
PF16656. Pur_ac_phosph_N. 1 hit.
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SIV9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRVRKSDFG SIVLVLCCVL NSLLCNGGIT SRYVRKLEAT VDMPLDSDVF
60 70 80 90 100
RVPCGYNAPQ QVHITQGDVE GKAVIVSWVT QEAKGSNKVI YWKENSTKKH
110 120 130 140 150
KAHGKTNTYK FYNYTSGFIH HCPIRNLEYD TKYYYVLGVG QTERKFWFFT
160 170 180 190 200
PPEIGPDVPY TFGLIGDLGQ SYDSNITLTH YENNPTKGQA VLFVGDISYA
210 220 230 240 250
DTYPDHDNRR WDSWGRFAER STAYQPWIWT TGNHELDFAP EIGENRPFKP
260 270 280 290 300
FTHRYRTPYR SSGSTEPFWY SIKRGPAYII VLASYSAYGK YTPQYQWLEE
310 320 330 340 350
EFPKVNRTET PWLIVLMHSP WYNSYDYHYM EGETMRVMYE AWFVKYKVDV
360 370 380 390 400
VFAGHVHAYE RSERVSNIAY NVVNGICTPV KDQSAPVYIT IGDGGNIEGL
410 420 430 440 450
ATKMTEPQPK YSAFREASFG HAIFSIKNRT HAHYGWHRNH DGYAVEGDRM
460
WFYNRFWHPV DDSPSCNS
Length:468
Mass (Da):54,219
Last modified:May 1, 2000 - v1
Checksum:iED1EB9821C5C118B
GO
Isoform 2 (identifier: Q9SIV9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: MGRVRKSDFG...IHHCPIRNLE → MLWFFLLQ

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:348
Mass (Da):40,909
Checksum:i84D390C33712D899
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167D → N in AAM16283 (PubMed:12021284).Curated1
Sequence conflicti280I → V in AAM16283 (PubMed:12021284).Curated1
Sequence conflicti441D → G in AAM16283 (PubMed:12021284).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0371921 – 128MGRVR…IRNLE → MLWFFLLQ in isoform 2. 1 PublicationAdd BLAST128

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492662 mRNA. Translation: AAM15911.1.
AY090893 mRNA. Translation: AAM16283.1.
AC007047 Genomic DNA. Translation: AAD22297.1.
CP002685 Genomic DNA. Translation: AEC06495.1.
CP002685 Genomic DNA. Translation: AEC06496.1.
AY093236 mRNA. Translation: AAM13235.1.
BT008761 mRNA. Translation: AAP49523.1.
PIRiB84540.
RefSeqiNP_179235.1. NM_127196.4. [Q9SIV9-1]
NP_849960.1. NM_179629.2. [Q9SIV9-2]
UniGeneiAt.14307.

Genome annotation databases

EnsemblPlantsiAT2G16430.1; AT2G16430.1; AT2G16430. [Q9SIV9-2]
AT2G16430.2; AT2G16430.2; AT2G16430. [Q9SIV9-1]
GeneIDi816141.
GrameneiAT2G16430.1; AT2G16430.1; AT2G16430.
AT2G16430.2; AT2G16430.2; AT2G16430.
KEGGiath:AT2G16430.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPPA10_ARATH
AccessioniPrimary (citable) accession number: Q9SIV9
Secondary accession number(s): Q8RX39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: October 25, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families