SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9SIV9

- PPA10_ARATH

UniProt

Q9SIV9 - PPA10_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Purple acid phosphatase 10
Gene
PAP10, AT11, At2g16430, F16F14.7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 iron ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi167 – 1671Iron By similarity
Metal bindingi196 – 1961Iron By similarity
Metal bindingi196 – 1961Zinc By similarity
Metal bindingi199 – 1991Iron By similarity
Metal bindingi233 – 2331Zinc By similarity
Binding sitei233 – 2331Substrate By similarity
Metal bindingi318 – 3181Zinc By similarity
Active sitei328 – 3281Proton donor By similarity
Metal bindingi355 – 3551Zinc By similarity
Metal bindingi357 – 3571Iron By similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular response to phosphate starvation Source: TAIR
  2. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G16430-MONOMER.
ARA:GQT-850-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 10 (EC:3.1.3.2)
Gene namesi
Name:PAP10
Synonyms:AT11
Ordered Locus Names:At2g16430
ORF Names:F16F14.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G16430.

Subcellular locationi

Isoform 1 : Secreted By similarity 1 Publication
Isoform 2 : Cytoplasm Inferred 1 Publication

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. cytosol Source: TAIR
  3. extracellular region Source: UniProtKB-SubCell
  4. plant-type cell wall Source: TAIR
  5. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 468443Purple acid phosphatase 10
PRO_0000372815Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi95 – 951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi113 – 1131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi175 – 1751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi306 – 3061N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi377 – 377Interchain By similarity
Glycosylationi428 – 4281N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9SIV9.
PRIDEiQ9SIV9.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Inductioni

By phosphate deprivation, mostly isoform 2.1 Publication

Gene expression databases

GenevestigatoriQ9SIV9.

Interactioni

Subunit structurei

Homodimer; disulfide-linked By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9SIV9.
SMRiQ9SIV9. Positions 42-463.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni355 – 3573Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238330.
InParanoidiQ9SIV9.
OMAiYKWLEEE.
PhylomeDBiQ9SIV9.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SIV9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGRVRKSDFG SIVLVLCCVL NSLLCNGGIT SRYVRKLEAT VDMPLDSDVF    50
RVPCGYNAPQ QVHITQGDVE GKAVIVSWVT QEAKGSNKVI YWKENSTKKH 100
KAHGKTNTYK FYNYTSGFIH HCPIRNLEYD TKYYYVLGVG QTERKFWFFT 150
PPEIGPDVPY TFGLIGDLGQ SYDSNITLTH YENNPTKGQA VLFVGDISYA 200
DTYPDHDNRR WDSWGRFAER STAYQPWIWT TGNHELDFAP EIGENRPFKP 250
FTHRYRTPYR SSGSTEPFWY SIKRGPAYII VLASYSAYGK YTPQYQWLEE 300
EFPKVNRTET PWLIVLMHSP WYNSYDYHYM EGETMRVMYE AWFVKYKVDV 350
VFAGHVHAYE RSERVSNIAY NVVNGICTPV KDQSAPVYIT IGDGGNIEGL 400
ATKMTEPQPK YSAFREASFG HAIFSIKNRT HAHYGWHRNH DGYAVEGDRM 450
WFYNRFWHPV DDSPSCNS 468
Length:468
Mass (Da):54,219
Last modified:May 1, 2000 - v1
Checksum:iED1EB9821C5C118B
GO
Isoform 2 (identifier: Q9SIV9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: MGRVRKSDFG...IHHCPIRNLE → MLWFFLLQ

Note: May be due to intron retention. No experimental confirmation available.

Show »
Length:348
Mass (Da):40,909
Checksum:i84D390C33712D899
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 128128MGRVR…IRNLE → MLWFFLLQ in isoform 2.
VSP_037192Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti167 – 1671D → N in AAM16283. 1 Publication
Sequence conflicti280 – 2801I → V in AAM16283. 1 Publication
Sequence conflicti441 – 4411D → G in AAM16283. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF492662 mRNA. Translation: AAM15911.1.
AY090893 mRNA. Translation: AAM16283.1.
AC007047 Genomic DNA. Translation: AAD22297.1.
CP002685 Genomic DNA. Translation: AEC06495.1.
CP002685 Genomic DNA. Translation: AEC06496.1.
AY093236 mRNA. Translation: AAM13235.1.
BT008761 mRNA. Translation: AAP49523.1.
PIRiB84540.
RefSeqiNP_179235.1. NM_127196.3. [Q9SIV9-1]
NP_849960.1. NM_179629.2. [Q9SIV9-2]
UniGeneiAt.14307.

Genome annotation databases

EnsemblPlantsiAT2G16430.2; AT2G16430.2; AT2G16430. [Q9SIV9-1]
GeneIDi816141.
KEGGiath:AT2G16430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF492662 mRNA. Translation: AAM15911.1 .
AY090893 mRNA. Translation: AAM16283.1 .
AC007047 Genomic DNA. Translation: AAD22297.1 .
CP002685 Genomic DNA. Translation: AEC06495.1 .
CP002685 Genomic DNA. Translation: AEC06496.1 .
AY093236 mRNA. Translation: AAM13235.1 .
BT008761 mRNA. Translation: AAP49523.1 .
PIRi B84540.
RefSeqi NP_179235.1. NM_127196.3. [Q9SIV9-1 ]
NP_849960.1. NM_179629.2. [Q9SIV9-2 ]
UniGenei At.14307.

3D structure databases

ProteinModelPortali Q9SIV9.
SMRi Q9SIV9. Positions 42-463.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9SIV9.
PRIDEi Q9SIV9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G16430.2 ; AT2G16430.2 ; AT2G16430 . [Q9SIV9-1 ]
GeneIDi 816141.
KEGGi ath:AT2G16430.

Organism-specific databases

TAIRi AT2G16430.

Phylogenomic databases

eggNOGi COG1409.
HOGENOMi HOG000238330.
InParanoidi Q9SIV9.
OMAi YKWLEEE.
PhylomeDBi Q9SIV9.

Enzyme and pathway databases

BioCyci ARA:AT2G16430-MONOMER.
ARA:GQT-850-MONOMER.

Gene expression databases

Genevestigatori Q9SIV9.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, INDUCTION, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Col-1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPPA10_ARATH
AccessioniPrimary (citable) accession number: Q9SIV9
Secondary accession number(s): Q8RX39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi