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Q9SIV9

- PPA10_ARATH

UniProt

Q9SIV9 - PPA10_ARATH

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Protein

Purple acid phosphatase 10

Gene

PAP10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi167 – 1671IronBy similarity
Metal bindingi196 – 1961IronBy similarity
Metal bindingi196 – 1961ZincBy similarity
Metal bindingi199 – 1991IronBy similarity
Metal bindingi233 – 2331ZincBy similarity
Binding sitei233 – 2331SubstrateBy similarity
Metal bindingi318 – 3181ZincBy similarity
Active sitei328 – 3281Proton donorBy similarity
Metal bindingi355 – 3551ZincBy similarity
Metal bindingi357 – 3571IronBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular response to phosphate starvation Source: TAIR
  2. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G16430-MONOMER.
ARA:GQT-850-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 10 (EC:3.1.3.2)
Gene namesi
Name:PAP10
Synonyms:AT11
Ordered Locus Names:At2g16430
ORF Names:F16F14.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G16430.

Subcellular locationi

Isoform 1 : Secreted By similarity

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. cytosol Source: TAIR
  3. extracellular region Source: UniProtKB-KW
  4. plant-type cell wall Source: TAIR
  5. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 468443Purple acid phosphatase 10PRO_0000372815Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi377 – 377InterchainBy similarity
Glycosylationi428 – 4281N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9SIV9.
PRIDEiQ9SIV9.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Inductioni

By phosphate deprivation, mostly isoform 2.1 Publication

Gene expression databases

ExpressionAtlasiQ9SIV9. baseline and differential.
GenevestigatoriQ9SIV9.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9SIV9.
SMRiQ9SIV9. Positions 42-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni355 – 3573Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238330.
InParanoidiQ9SIV9.
OMAiYKWLEEE.
PhylomeDBiQ9SIV9.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SIV9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRVRKSDFG SIVLVLCCVL NSLLCNGGIT SRYVRKLEAT VDMPLDSDVF
60 70 80 90 100
RVPCGYNAPQ QVHITQGDVE GKAVIVSWVT QEAKGSNKVI YWKENSTKKH
110 120 130 140 150
KAHGKTNTYK FYNYTSGFIH HCPIRNLEYD TKYYYVLGVG QTERKFWFFT
160 170 180 190 200
PPEIGPDVPY TFGLIGDLGQ SYDSNITLTH YENNPTKGQA VLFVGDISYA
210 220 230 240 250
DTYPDHDNRR WDSWGRFAER STAYQPWIWT TGNHELDFAP EIGENRPFKP
260 270 280 290 300
FTHRYRTPYR SSGSTEPFWY SIKRGPAYII VLASYSAYGK YTPQYQWLEE
310 320 330 340 350
EFPKVNRTET PWLIVLMHSP WYNSYDYHYM EGETMRVMYE AWFVKYKVDV
360 370 380 390 400
VFAGHVHAYE RSERVSNIAY NVVNGICTPV KDQSAPVYIT IGDGGNIEGL
410 420 430 440 450
ATKMTEPQPK YSAFREASFG HAIFSIKNRT HAHYGWHRNH DGYAVEGDRM
460
WFYNRFWHPV DDSPSCNS
Length:468
Mass (Da):54,219
Last modified:May 1, 2000 - v1
Checksum:iED1EB9821C5C118B
GO
Isoform 2 (identifier: Q9SIV9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: MGRVRKSDFG...IHHCPIRNLE → MLWFFLLQ

Note: May be due to intron retention. No experimental confirmation available.

Show »
Length:348
Mass (Da):40,909
Checksum:i84D390C33712D899
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti167 – 1671D → N in AAM16283. (PubMed:12021284)Curated
Sequence conflicti280 – 2801I → V in AAM16283. (PubMed:12021284)Curated
Sequence conflicti441 – 4411D → G in AAM16283. (PubMed:12021284)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 128128MGRVR…IRNLE → MLWFFLLQ in isoform 2. 1 PublicationVSP_037192Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492662 mRNA. Translation: AAM15911.1.
AY090893 mRNA. Translation: AAM16283.1.
AC007047 Genomic DNA. Translation: AAD22297.1.
CP002685 Genomic DNA. Translation: AEC06495.1.
CP002685 Genomic DNA. Translation: AEC06496.1.
AY093236 mRNA. Translation: AAM13235.1.
BT008761 mRNA. Translation: AAP49523.1.
PIRiB84540.
RefSeqiNP_179235.1. NM_127196.3. [Q9SIV9-1]
NP_849960.1. NM_179629.2. [Q9SIV9-2]
UniGeneiAt.14307.

Genome annotation databases

EnsemblPlantsiAT2G16430.2; AT2G16430.2; AT2G16430. [Q9SIV9-1]
GeneIDi816141.
KEGGiath:AT2G16430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492662 mRNA. Translation: AAM15911.1 .
AY090893 mRNA. Translation: AAM16283.1 .
AC007047 Genomic DNA. Translation: AAD22297.1 .
CP002685 Genomic DNA. Translation: AEC06495.1 .
CP002685 Genomic DNA. Translation: AEC06496.1 .
AY093236 mRNA. Translation: AAM13235.1 .
BT008761 mRNA. Translation: AAP49523.1 .
PIRi B84540.
RefSeqi NP_179235.1. NM_127196.3. [Q9SIV9-1 ]
NP_849960.1. NM_179629.2. [Q9SIV9-2 ]
UniGenei At.14307.

3D structure databases

ProteinModelPortali Q9SIV9.
SMRi Q9SIV9. Positions 42-463.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9SIV9.
PRIDEi Q9SIV9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G16430.2 ; AT2G16430.2 ; AT2G16430 . [Q9SIV9-1 ]
GeneIDi 816141.
KEGGi ath:AT2G16430.

Organism-specific databases

TAIRi AT2G16430.

Phylogenomic databases

eggNOGi COG1409.
HOGENOMi HOG000238330.
InParanoidi Q9SIV9.
OMAi YKWLEEE.
PhylomeDBi Q9SIV9.

Enzyme and pathway databases

BioCyci ARA:AT2G16430-MONOMER.
ARA:GQT-850-MONOMER.

Gene expression databases

ExpressionAtlasi Q9SIV9. baseline and differential.
Genevestigatori Q9SIV9.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, INDUCTION, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Col-1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPPA10_ARATH
AccessioniPrimary (citable) accession number: Q9SIV9
Secondary accession number(s): Q8RX39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3