Reviewed, UniProtKB/Swiss-Prot Q9SIV9 (PPA10_ARATH)

Names and origin

Protein names

Recommended name:
    Purple acid phosphatase 10
    EC=3.1.3.2

Gene names

Name:	PAP10
Synonyms:	AT11
Ordered Locus Names:	At2g16430
ORF Names:	F16F14.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Isoform 1: Secreted By similarity. Isoform 2: Cytoplasm Probable.
Tissue specificity	Expressed in roots, stems, leaves, flowers and siliques. Ref.4
Induction	By phosphate deprivation, mostly isoform 2. Ref.1
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
Cellular component	Cytoplasm Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type cell wall Inferred from direct assay. Source: TAIR
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9SIV9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9SIV9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: MGRVRKSDFG...IHHCPIRNLE → MLWFFLLQ

Note: May be due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 468	443	Purple acid phosphatase 10		PRO_0000372815
Regions
	Region	355 – 357	3	Substrate binding By similarity
Sites
	Active site	328	1	Proton donor By similarity
	Metal binding	167	1	Iron By similarity
	Metal binding	196	1	Iron By similarity
	Metal binding	196	1	Zinc By similarity
	Metal binding	199	1	Iron By similarity
	Metal binding	233	1	Zinc By similarity
	Metal binding	318	1	Zinc By similarity
	Metal binding	355	1	Zinc By similarity
	Metal binding	357	1	Iron By similarity
	Binding site	233	1	Substrate By similarity
Amino acid modifications
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Glycosylation	175	1	N-linked (GlcNAc...) Potential
	Glycosylation	306	1	N-linked (GlcNAc...) Potential
	Glycosylation	428	1	N-linked (GlcNAc...) Potential
	Disulfide bond	377		Interchain By similarity
Natural variations
	Alternative sequence	1 – 128	128	MGRVR…IRNLE → MLWFFLLQ in isoform 2.		VSP_037192
Experimental info
	Sequence conflict	167	1	D → N in AAM16283. Ref.1
	Sequence conflict	280	1	I → V in AAM16283. Ref.1
	Sequence conflict	441	1	D → G in AAM16283. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: ED1EB9821C5C118B	FASTA	468	54,219
	10 20 30 40 50 60 MGRVRKSDFG SIVLVLCCVL NSLLCNGGIT SRYVRKLEAT VDMPLDSDVF RVPCGYNAPQ 70 80 90 100 110 120 QVHITQGDVE GKAVIVSWVT QEAKGSNKVI YWKENSTKKH KAHGKTNTYK FYNYTSGFIH 130 140 150 160 170 180 HCPIRNLEYD TKYYYVLGVG QTERKFWFFT PPEIGPDVPY TFGLIGDLGQ SYDSNITLTH 190 200 210 220 230 240 YENNPTKGQA VLFVGDISYA DTYPDHDNRR WDSWGRFAER STAYQPWIWT TGNHELDFAP 250 260 270 280 290 300 EIGENRPFKP FTHRYRTPYR SSGSTEPFWY SIKRGPAYII VLASYSAYGK YTPQYQWLEE 310 320 330 340 350 360 EFPKVNRTET PWLIVLMHSP WYNSYDYHYM EGETMRVMYE AWFVKYKVDV VFAGHVHAYE 370 380 390 400 410 420 RSERVSNIAY NVVNGICTPV KDQSAPVYIT IGDGGNIEGL ATKMTEPQPK YSAFREASFG 430 440 450 460 HAIFSIKNRT HAHYGWHRNH DGYAVEGDRM WFYNRFWHPV DDSPSCNS
	Isoform 2. Checksum: 84D390C33712D899	FASTA	348	40,909
	10 20 30 40 50 60 MLWFFLLQYD TKYYYVLGVG QTERKFWFFT PPEIGPDVPY TFGLIGDLGQ SYDSNITLTH 70 80 90 100 110 120 YENNPTKGQA VLFVGDISYA DTYPDHDNRR WDSWGRFAER STAYQPWIWT TGNHELDFAP 130 140 150 160 170 180 EIGENRPFKP FTHRYRTPYR SSGSTEPFWY SIKRGPAYII VLASYSAYGK YTPQYQWLEE 190 200 210 220 230 240 EFPKVNRTET PWLIVLMHSP WYNSYDYHYM EGETMRVMYE AWFVKYKVDV VFAGHVHAYE 250 260 270 280 290 300 RSERVSNIAY NVVNGICTPV KDQSAPVYIT IGDGGNIEGL ATKMTEPQPK YSAFREASFG 310 320 330 340 HAIFSIKNRT HAHYGWHRNH DGYAVEGDRM WFYNRFWHPV DDSPSCNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, INDUCTION, GENE FAMILY, NOMENCLATURE. Strain: cv. Col-1.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed: 16244908] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF492662 mRNA. Translation: AAM15911.1. AY090893 mRNA. Translation: AAM16283.1. AC007047 Genomic DNA. Translation: AAD22297.1. AY093236 mRNA. Translation: AAM13235.1. BT008761 mRNA. Translation: AAP49523.1.
IPI	IPI00530753. IPI00539645.
PIR	B84540.
RefSeq	NP_179235.1. NP_849960.1.
UniGene	At.14307
3D structure databases
HSSP	HSSP built from PDB template 4KBP based on UniProtKB P80366.
SMR	Q9SIV9. Positions 42-463.
ModBase	Search...
Proteomic databases
PRIDE	Q9SIV9.
Genome annotation databases
GeneID	816141.
GenomeReviews	Gene locus AT2G16430 in contig CT485783_GR.
KEGG	ath:AT2G16430.
NMPDR	fig|3702.1.peg.8632.
Organism-specific databases
TAIR	At2g16430.
Phylogenomic databases
OMA	EFPKVNR.
Gene expression databases
ArrayExpress	Q9SIV9.
Genevestigator	Q9SIV9.
Family and domain databases
InterPro	IPR004843. M-pesterase. IPR015914. Purple_acid_Pase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.380. Purple_acid_Pase_N. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA10_ARATH

Accession

Primary (citable) accession number: Q9SIV9
Secondary accession number(s): Q8RX39

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)