Q9SIV2 (RPN1A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
26S proteasome non-ATPase regulatory subunit 2 1A

Alternative name(s):
26S proteasome regulatory subunit RPN1 A
Short name=AtRPN1a
26S proteasome regulatory subunit S2 1A

Gene names

Name:	RPN1A
Ordered Locus Names:	At2g20580
ORF Names:	F23N11.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	891 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins By similarity. Required during embryogenesis (Ref.6). Required for optimal plant growth and stress responses (Ref.7). Ref.6 Ref.7
Tissue specificity	Expressed in stems, leaves, buds, flowers, siliques and developing seeds. Ref.6
Developmental stage	Detected in the floral meristem and primordia of floral organs. During early stages of flower development, expressed in the whole floral meristem, especially in emerging primordia. Later present in developing carpels, particularly in emerging ovule primordia. After fertilization, observed in the embryo and the chalazal endosperm. High levels at the early embryogenesis stages that fade out later. Also present in the developing seed coat, the septum, and the silique wall. In the stamens, detected at high levels in the anthers. Observed in sporogenous cells and then in tetrads of microspores. Ref.6
Post-translational modification	Ubiquitinated.
Disruption phenotype	Embryo lethality due to development arrest at the globular stage with defects in the formation of the embryonic root, the protoderm, and procambium (Ref.6). Increased cell sizes, anthocyanin accumulation, reduced growth rate, reduced fertility, decreased heat shock tolerance, increased oxidative stress tolerance (Ref.7). Ref.6 Ref.7
Sequence similarities	Belongs to the proteasome subunit S2 family. Contains 7 PC repeats.
Caution	The embryo lethal disruption phenotype (Ref.6) could be confirmed (Ref.7).
Sequence caution	The sequence BAD94334.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Proteasome
Domain	Repeat
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein catabolic process Traceable author statement PubMed 11742986. Source: TAIR regulation of catalytic activity Inferred from electronic annotation. Source: GOC regulation of cell cycle Inferred from mutant phenotype Ref.6. Source: TAIR regulation of protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR nucleus Traceable author statement PubMed 11742986. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR proteasome complex Inferred from direct assay PubMed 20516081. Source: TAIR
Molecular_function	enzyme regulator activity Inferred from electronic annotation. Source: InterPro ubiquitin binding Inferred from direct assay Ref.8. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 891	891	26S proteasome non-ATPase regulatory subunit 2 1A		PRO_0000399921
Regions
	Repeat	414 – 447	34	PC 1
	Repeat	448 – 484	37	PC 2
	Repeat	485 – 519	35	PC 3
	Repeat	522 – 556	35	PC 4
	Repeat	565 – 594	30	PC 5
	Repeat	674 – 705	32	PC 6
	Repeat	724 – 739	16	PC 7
Amino acid modifications
	Modified residue	84	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SIV2 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 68C0B94F83F21955	FASTA	891	98,144
	10 20 30 40 50 60 MAPTQDPNSV GGGAKKDEAT LKVPSKDPKK KDEKKDEDLS EEDLELKQNL ELYVERVQDP 70 80 90 100 110 120 NPELQKAALE SMRQEIRAST SSMTSVPKPL KFLRPHYGTL KAFHETMADS DLKKYLSDIL 130 140 150 160 170 180 SVLALTMSAD GERESLRFRL IGTEGDIGSW GHEYVRNLAG EIAQEYTKRQ SEEASIDDLM 190 200 210 220 230 240 ELVQQIVAFH MKHNAETEAV DLLMDVEDLD LLLEHVDKTN FKRTCNYLTS AARYLPGPDD 250 260 270 280 290 300 MLVLDISYMI YMKFEEYPNA LQIALFLDNT QYVKQVFTSC TDLLKKKQFC YMIARHGITF 310 320 330 340 350 360 ELDDEMVADD DDREALQDIV NNTKLSEGYL TLARDIEVME AKTPEDIYKA HLLDGRASSG 370 380 390 400 410 420 ASVDSARQNL AATFVNAFVN AGFGQDKLMT VPSDSTTGSS GNWLFKNKEH GKTSAAASLG 430 440 450 460 470 480 MIQLWDVDSG LSQLDKYFHS NDNPIIAGAL LGVGIVNCGI KNDCDPALAL LGDYIDKEDS 490 500 510 520 530 540 SVRIGAIMGL GISYAGSQND QIRNKLSPIL NDAKAPLDVI AFASLSLGMI YVGSCNEEVA 550 560 570 580 590 600 QSIIFALMDR SEAELGDALT RFLPLGLGLL YLGKQESVEA TAEVSKTFNE KIRKYCDMTL 610 620 630 640 650 660 LSCAYAGTGN VLKVQDLLAQ CGEHLEKGDI HQGPAVLGLA MVAMSEELGV DMEIRSLERM 670 680 690 700 710 720 LQYGEQNIRR AVPLALGLLC ISNPKVTVMD TLSRLSHDTD SEVAMSAIIS LGLIGAGTNN 730 740 750 760 770 780 ARIAGMLRNL SSYYYKDMSL LFCVRIAQGL VHMGKGLLTL SPFHSERFLL SPTALAGIVT 790 800 810 820 830 840 LLHACLDMKP IILGKYHYVL YFLVLAMQPR MMLTVDENLK PLSVPVRVGQ AVDVVGQAGR 850 860 870 880 890 PKTITGFQTH STPVLLAAGE RAELATDKYI PLSPILEGFI ILKENPDYRE E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms." Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D. J. Biol. Chem. 279:6401-6413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 643-891. Strain: cv. Columbia.
[6]	"The RPN1 subunit of the 26S proteasome in Arabidopsis is essential for embryogenesis." Brukhin V., Gheyselinck J., Gagliardini V., Genschik P., Grossniklaus U. Plant Cell 17:2723-2737(2005) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[7]	"The Arabidopsis 26S proteasome subunit RPN1a is required for optimal plant growth and stress responses." Wang S., Kurepa J., Smalle J.A. Plant Cell Physiol. 50:1721-1725(2009) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[8]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY230828 mRNA. Translation: AAP86655.1. AC007048 Genomic DNA. Translation: AAD21708.2. CP002685 Genomic DNA. Translation: AEC07032.1. AF360216 mRNA. Translation: AAK25926.1. AY040061 mRNA. Translation: AAK64119.1. AK220903 mRNA. Translation: BAD94334.1. Different initiation.
IPI	IPI00529314.
PIR	A84591.
RefSeq	NP_565477.1. NM_127618.3.
UniGene	At.22879.
3D structure databases
ProteinModelPortal	Q9SIV2.
SMR	Q9SIV2. Positions 346-779.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SIV2. 28 interactions.
Proteomic databases
PaxDb	Q9SIV2.
PRIDE	Q9SIV2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G20580.1; AT2G20580.1; AT2G20580.
GeneID	816580.
KEGG	ath:AT2G20580.
Organism-specific databases
TAIR	At2g20580.
Phylogenomic databases
eggNOG	COG5110.
HOGENOM	HOG000176022.
InParanoid	Q9SIV2.
KO	K03028.
OMA	IAQGLTH.
PhylomeDB	Q9SIV2.
ProtClustDB	CLSN2688277.
Gene expression databases
Genevestigator	Q9SIV2.
Family and domain databases
Gene3D	1.25.10.10. 3 hits.
InterPro	IPR016643. 26S_Psome_Rpn1. IPR002015. APC_proteasome. IPR011989. ARM-like. IPR016024. ARM-type_fold. [Graphical view]
PANTHER	PTHR10943:SF1. PTHR10943:SF1. 1 hit.
Pfam	PF01851. PC_rep. 2 hits. [Graphical view]
PIRSF	PIRSF015965. 26S_Psome_Rpn1. 1 hit.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPN1A_ARATH

Accession

Primary (citable) accession number: Q9SIV2
Secondary accession number(s): Q56ZR0, Q9C5J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program