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Q9SIV2

- PSD2A_ARATH

UniProt

Q9SIV2 - PSD2A_ARATH

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Protein

26S proteasome non-ATPase regulatory subunit 2 homolog A

Gene

RPN1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins (By similarity). Required during embryogenesis (PubMed:16169895). Required for optimal plant growth and stress responses (PubMed:19605416). Required for innate immunity (PubMed:22577987).By similarity3 Publications

GO - Molecular functioni

  1. enzyme regulator activity Source: InterPro
  2. ubiquitin binding Source: UniProtKB

GO - Biological processi

  1. innate immune response Source: UniProtKB
  2. protein catabolic process Source: TAIR
  3. regulation of cell cycle Source: TAIR
  4. regulation of protein catabolic process Source: InterPro
  5. response to salicylic acid Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_107429. Orc1 removal from chromatin.
REACT_185297. Separation of Sister Chromatids.
REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_187719. ER-Phagosome pathway.
REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_240263. Ubiquitin-dependent degradation of Cyclin D1.
REACT_241531. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_244054. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_252436. CDK-mediated phosphorylation and removal of Cdc6.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 2 homolog A
Alternative name(s):
26S proteasome regulatory subunit RPN1a
Short name:
AtRPN1a
26S proteasome regulatory subunit S2 homolog A
Gene namesi
Name:RPN1A
Ordered Locus Names:At2g20580
ORF Names:F23N11.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G20580.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: TAIR
  3. nucleus Source: UniProtKB
  4. plasma membrane Source: TAIR
  5. plasmodesma Source: TAIR
  6. proteasome complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Disruption phenotypei

Embryo lethality due to development arrest at the globular stage with defects in the formation of the embryonic root, the protoderm, and procambium (PubMed:16169895). Increased cell sizes, anthocyanin accumulation, reduced growth rate, reduced fertility, decreased heat shock tolerance, increased oxidative stress tolerance (PubMed:19605416). Mutant displays enhanced susceptibility to the fungal pathogen G. cichoracearum and to virulent and avirulent bacterial Pto DC3000 strains, which indicated defects in basal defense and resistance (R) protein-mediated defense (PubMed:22577987).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 89189126S proteasome non-ATPase regulatory subunit 2 homolog APRO_0000399921Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki218 – 218Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei219 – 2191N-acetylthreonine1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9SIV2.
PRIDEiQ9SIV2.

Expressioni

Tissue specificityi

Expressed in stems, leaves, buds, flowers, siliques and developing seeds.2 Publications

Developmental stagei

Detected in the floral meristem and primordia of floral organs. During early stages of flower development, expressed in the whole floral meristem, especially in emerging primordia. Later present in developing carpels, particularly in emerging ovule primordia. After fertilization, observed in the embryo and the chalazal endosperm. High levels at the early embryogenesis stages that fade out later. Also present in the developing seed coat, the septum, and the silique wall. In the stamens, detected at high levels in the anthers. Observed in sporogenous cells and then in tetrads of microspores.1 Publication

Inductioni

By salicylic acid (SA).1 Publication

Gene expression databases

GenevestigatoriQ9SIV2.

Interactioni

Subunit structurei

Component of the 19S regulatory particule (RP/PA700) base subcomplex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The RP/PA700 complex is composed of at least 17 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively.2 Publications

Protein-protein interaction databases

BioGridi1933. 28 interactions.
IntActiQ9SIV2. 28 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9SIV2.
SMRiQ9SIV2. Positions 50-891.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati414 – 44734PC 1Add
BLAST
Repeati448 – 48437PC 2Add
BLAST
Repeati485 – 51935PC 3Add
BLAST
Repeati522 – 55635PC 4Add
BLAST
Repeati565 – 59430PC 5Add
BLAST
Repeati674 – 70532PC 6Add
BLAST
Repeati724 – 73916PC 7Add
BLAST

Sequence similaritiesi

Belongs to the proteasome subunit S2 family.Curated
Contains 7 PC repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5110.
HOGENOMiHOG000176022.
InParanoidiQ9SIV2.
KOiK03028.
OMAiIAQGLTH.
PhylomeDBiQ9SIV2.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR016643. 26S_Psome_Rpn1.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002015. Proteasome/cyclosome_rpt.
[Graphical view]
PANTHERiPTHR10943:SF1. PTHR10943:SF1. 1 hit.
PfamiPF01851. PC_rep. 2 hits.
[Graphical view]
PIRSFiPIRSF015965. 26S_Psome_Rpn1. 1 hit.
SUPFAMiSSF48371. SSF48371. 4 hits.

Sequencei

Sequence statusi: Complete.

Q9SIV2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPTQDPNSV GGGAKKDEAT LKVPSKDPKK KDEKKDEDLS EEDLELKQNL
60 70 80 90 100
ELYVERVQDP NPELQKAALE SMRQEIRAST SSMTSVPKPL KFLRPHYGTL
110 120 130 140 150
KAFHETMADS DLKKYLSDIL SVLALTMSAD GERESLRFRL IGTEGDIGSW
160 170 180 190 200
GHEYVRNLAG EIAQEYTKRQ SEEASIDDLM ELVQQIVAFH MKHNAETEAV
210 220 230 240 250
DLLMDVEDLD LLLEHVDKTN FKRTCNYLTS AARYLPGPDD MLVLDISYMI
260 270 280 290 300
YMKFEEYPNA LQIALFLDNT QYVKQVFTSC TDLLKKKQFC YMIARHGITF
310 320 330 340 350
ELDDEMVADD DDREALQDIV NNTKLSEGYL TLARDIEVME AKTPEDIYKA
360 370 380 390 400
HLLDGRASSG ASVDSARQNL AATFVNAFVN AGFGQDKLMT VPSDSTTGSS
410 420 430 440 450
GNWLFKNKEH GKTSAAASLG MIQLWDVDSG LSQLDKYFHS NDNPIIAGAL
460 470 480 490 500
LGVGIVNCGI KNDCDPALAL LGDYIDKEDS SVRIGAIMGL GISYAGSQND
510 520 530 540 550
QIRNKLSPIL NDAKAPLDVI AFASLSLGMI YVGSCNEEVA QSIIFALMDR
560 570 580 590 600
SEAELGDALT RFLPLGLGLL YLGKQESVEA TAEVSKTFNE KIRKYCDMTL
610 620 630 640 650
LSCAYAGTGN VLKVQDLLAQ CGEHLEKGDI HQGPAVLGLA MVAMSEELGV
660 670 680 690 700
DMEIRSLERM LQYGEQNIRR AVPLALGLLC ISNPKVTVMD TLSRLSHDTD
710 720 730 740 750
SEVAMSAIIS LGLIGAGTNN ARIAGMLRNL SSYYYKDMSL LFCVRIAQGL
760 770 780 790 800
VHMGKGLLTL SPFHSERFLL SPTALAGIVT LLHACLDMKP IILGKYHYVL
810 820 830 840 850
YFLVLAMQPR MMLTVDENLK PLSVPVRVGQ AVDVVGQAGR PKTITGFQTH
860 870 880 890
STPVLLAAGE RAELATDKYI PLSPILEGFI ILKENPDYRE E
Length:891
Mass (Da):98,144
Last modified:June 1, 2002 - v2
Checksum:i68C0B94F83F21955
GO

Sequence cautioni

The sequence BAD94334.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY230828 mRNA. Translation: AAP86655.1.
AC007048 Genomic DNA. Translation: AAD21708.2.
CP002685 Genomic DNA. Translation: AEC07032.1.
AF360216 mRNA. Translation: AAK25926.1.
AY040061 mRNA. Translation: AAK64119.1.
AK220903 mRNA. Translation: BAD94334.1. Different initiation.
PIRiA84591.
RefSeqiNP_565477.1. NM_127618.3.
UniGeneiAt.22879.

Genome annotation databases

EnsemblPlantsiAT2G20580.1; AT2G20580.1; AT2G20580.
GeneIDi816580.
KEGGiath:AT2G20580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY230828 mRNA. Translation: AAP86655.1 .
AC007048 Genomic DNA. Translation: AAD21708.2 .
CP002685 Genomic DNA. Translation: AEC07032.1 .
AF360216 mRNA. Translation: AAK25926.1 .
AY040061 mRNA. Translation: AAK64119.1 .
AK220903 mRNA. Translation: BAD94334.1 . Different initiation.
PIRi A84591.
RefSeqi NP_565477.1. NM_127618.3.
UniGenei At.22879.

3D structure databases

ProteinModelPortali Q9SIV2.
SMRi Q9SIV2. Positions 50-891.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 1933. 28 interactions.
IntActi Q9SIV2. 28 interactions.

Proteomic databases

PaxDbi Q9SIV2.
PRIDEi Q9SIV2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G20580.1 ; AT2G20580.1 ; AT2G20580 .
GeneIDi 816580.
KEGGi ath:AT2G20580.

Organism-specific databases

TAIRi AT2G20580.

Phylogenomic databases

eggNOGi COG5110.
HOGENOMi HOG000176022.
InParanoidi Q9SIV2.
KOi K03028.
OMAi IAQGLTH.
PhylomeDBi Q9SIV2.

Enzyme and pathway databases

Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_107429. Orc1 removal from chromatin.
REACT_185297. Separation of Sister Chromatids.
REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_187719. ER-Phagosome pathway.
REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_240263. Ubiquitin-dependent degradation of Cyclin D1.
REACT_241531. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_244054. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_252436. CDK-mediated phosphorylation and removal of Cdc6.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Miscellaneous databases

PROi Q9SIV2.

Gene expression databases

Genevestigatori Q9SIV2.

Family and domain databases

Gene3Di 1.25.10.10. 3 hits.
InterProi IPR016643. 26S_Psome_Rpn1.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002015. Proteasome/cyclosome_rpt.
[Graphical view ]
PANTHERi PTHR10943:SF1. PTHR10943:SF1. 1 hit.
Pfami PF01851. PC_rep. 2 hits.
[Graphical view ]
PIRSFi PIRSF015965. 26S_Psome_Rpn1. 1 hit.
SUPFAMi SSF48371. SSF48371. 4 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms."
    Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D.
    J. Biol. Chem. 279:6401-6413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 643-891.
    Strain: cv. Columbia.
  6. "The RPN1 subunit of the 26S proteasome in Arabidopsis is essential for embryogenesis."
    Brukhin V., Gheyselinck J., Gagliardini V., Genschik P., Grossniklaus U.
    Plant Cell 17:2723-2737(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. "The Arabidopsis 26S proteasome subunit RPN1a is required for optimal plant growth and stress responses."
    Wang S., Kurepa J., Smalle J.A.
    Plant Cell Physiol. 50:1721-1725(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  8. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
  9. "Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
    Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
    J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT, ACETYLATION AT THR-219, UBIQUITINATION AT LYS-218.
  10. "RPN1a, a 26S proteasome subunit, is required for innate immunity in Arabidopsis."
    Yao C., Wu Y., Nie H., Tang D.
    Plant J. 71:1015-1028(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INDUCTION BY SALICYLIC ACID.

Entry informationi

Entry nameiPSD2A_ARATH
AccessioniPrimary (citable) accession number: Q9SIV2
Secondary accession number(s): Q56ZR0, Q9C5J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

The embryo lethal disruption phenotype (PubMed:16169895) could be confirmed (PubMed:19605416).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3