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Q9SIV2

- PSD2A_ARATH

UniProt

Q9SIV2 - PSD2A_ARATH

Protein

26S proteasome non-ATPase regulatory subunit 2 homolog A

Gene

RPN1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins By similarity. Required during embryogenesis (PubMed:16169895). Required for optimal plant growth and stress responses (PubMed:19605416). Required for innate immunity (PubMed:22577987).By similarity3 Publications

    GO - Molecular functioni

    1. enzyme regulator activity Source: InterPro
    2. protein binding Source: TAIR
    3. ubiquitin binding Source: UniProtKB

    GO - Biological processi

    1. innate immune response Source: UniProtKB
    2. protein catabolic process Source: TAIR
    3. regulation of cell cycle Source: TAIR
    4. regulation of protein catabolic process Source: InterPro
    5. response to salicylic acid Source: UniProtKB

    Keywords - Biological processi

    Immunity, Innate immunity

    Enzyme and pathway databases

    ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_107429. Orc1 removal from chromatin.
    REACT_185297. Separation of Sister Chromatids.
    REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_187719. ER-Phagosome pathway.
    REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_190947. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    26S proteasome non-ATPase regulatory subunit 2 homolog A
    Alternative name(s):
    26S proteasome regulatory subunit RPN1a
    Short name:
    AtRPN1a
    26S proteasome regulatory subunit S2 homolog A
    Gene namesi
    Name:RPN1A
    Ordered Locus Names:At2g20580
    ORF Names:F23N11.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G20580.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytosol Source: TAIR
    3. nucleus Source: UniProtKB
    4. plasma membrane Source: TAIR
    5. plasmodesma Source: TAIR
    6. proteasome complex Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    Pathology & Biotechi

    Disruption phenotypei

    Embryo lethality due to development arrest at the globular stage with defects in the formation of the embryonic root, the protoderm, and procambium (PubMed:16169895). Increased cell sizes, anthocyanin accumulation, reduced growth rate, reduced fertility, decreased heat shock tolerance, increased oxidative stress tolerance (PubMed:19605416). Mutant displays enhanced susceptibility to the fungal pathogen G. cichoracearum and to virulent and avirulent bacterial Pto DC3000 strains, which indicated defects in basal defense and resistance (R) protein-mediated defense (PubMed:22577987).3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 89189126S proteasome non-ATPase regulatory subunit 2 homolog APRO_0000399921Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki218 – 218Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)2 Publications
    Modified residuei219 – 2191N-acetylthreonine1 Publication

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiQ9SIV2.
    PRIDEiQ9SIV2.

    Expressioni

    Tissue specificityi

    Expressed in stems, leaves, buds, flowers, siliques and developing seeds.2 Publications

    Developmental stagei

    Detected in the floral meristem and primordia of floral organs. During early stages of flower development, expressed in the whole floral meristem, especially in emerging primordia. Later present in developing carpels, particularly in emerging ovule primordia. After fertilization, observed in the embryo and the chalazal endosperm. High levels at the early embryogenesis stages that fade out later. Also present in the developing seed coat, the septum, and the silique wall. In the stamens, detected at high levels in the anthers. Observed in sporogenous cells and then in tetrads of microspores.1 Publication

    Inductioni

    By salicylic acid (SA).1 Publication

    Gene expression databases

    GenevestigatoriQ9SIV2.

    Interactioni

    Subunit structurei

    Component of the 19S regulatory particule (RP/PA700) base subcomplex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The RP/PA700 complex is composed of at least 17 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively.2 Publications

    Protein-protein interaction databases

    BioGridi1933. 28 interactions.
    IntActiQ9SIV2. 28 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SIV2.
    SMRiQ9SIV2. Positions 50-891.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati414 – 44734PC 1Add
    BLAST
    Repeati448 – 48437PC 2Add
    BLAST
    Repeati485 – 51935PC 3Add
    BLAST
    Repeati522 – 55635PC 4Add
    BLAST
    Repeati565 – 59430PC 5Add
    BLAST
    Repeati674 – 70532PC 6Add
    BLAST
    Repeati724 – 73916PC 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the proteasome subunit S2 family.Curated
    Contains 7 PC repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5110.
    HOGENOMiHOG000176022.
    InParanoidiQ9SIV2.
    KOiK03028.
    OMAiIAQGLTH.
    PhylomeDBiQ9SIV2.

    Family and domain databases

    Gene3Di1.25.10.10. 3 hits.
    InterProiIPR016643. 26S_Psome_Rpn1.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002015. Proteasome/cyclosome_rpt.
    [Graphical view]
    PANTHERiPTHR10943:SF1. PTHR10943:SF1. 1 hit.
    PfamiPF01851. PC_rep. 2 hits.
    [Graphical view]
    PIRSFiPIRSF015965. 26S_Psome_Rpn1. 1 hit.
    SUPFAMiSSF48371. SSF48371. 4 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9SIV2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPTQDPNSV GGGAKKDEAT LKVPSKDPKK KDEKKDEDLS EEDLELKQNL    50
    ELYVERVQDP NPELQKAALE SMRQEIRAST SSMTSVPKPL KFLRPHYGTL 100
    KAFHETMADS DLKKYLSDIL SVLALTMSAD GERESLRFRL IGTEGDIGSW 150
    GHEYVRNLAG EIAQEYTKRQ SEEASIDDLM ELVQQIVAFH MKHNAETEAV 200
    DLLMDVEDLD LLLEHVDKTN FKRTCNYLTS AARYLPGPDD MLVLDISYMI 250
    YMKFEEYPNA LQIALFLDNT QYVKQVFTSC TDLLKKKQFC YMIARHGITF 300
    ELDDEMVADD DDREALQDIV NNTKLSEGYL TLARDIEVME AKTPEDIYKA 350
    HLLDGRASSG ASVDSARQNL AATFVNAFVN AGFGQDKLMT VPSDSTTGSS 400
    GNWLFKNKEH GKTSAAASLG MIQLWDVDSG LSQLDKYFHS NDNPIIAGAL 450
    LGVGIVNCGI KNDCDPALAL LGDYIDKEDS SVRIGAIMGL GISYAGSQND 500
    QIRNKLSPIL NDAKAPLDVI AFASLSLGMI YVGSCNEEVA QSIIFALMDR 550
    SEAELGDALT RFLPLGLGLL YLGKQESVEA TAEVSKTFNE KIRKYCDMTL 600
    LSCAYAGTGN VLKVQDLLAQ CGEHLEKGDI HQGPAVLGLA MVAMSEELGV 650
    DMEIRSLERM LQYGEQNIRR AVPLALGLLC ISNPKVTVMD TLSRLSHDTD 700
    SEVAMSAIIS LGLIGAGTNN ARIAGMLRNL SSYYYKDMSL LFCVRIAQGL 750
    VHMGKGLLTL SPFHSERFLL SPTALAGIVT LLHACLDMKP IILGKYHYVL 800
    YFLVLAMQPR MMLTVDENLK PLSVPVRVGQ AVDVVGQAGR PKTITGFQTH 850
    STPVLLAAGE RAELATDKYI PLSPILEGFI ILKENPDYRE E 891
    Length:891
    Mass (Da):98,144
    Last modified:June 1, 2002 - v2
    Checksum:i68C0B94F83F21955
    GO

    Sequence cautioni

    The sequence BAD94334.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY230828 mRNA. Translation: AAP86655.1.
    AC007048 Genomic DNA. Translation: AAD21708.2.
    CP002685 Genomic DNA. Translation: AEC07032.1.
    AF360216 mRNA. Translation: AAK25926.1.
    AY040061 mRNA. Translation: AAK64119.1.
    AK220903 mRNA. Translation: BAD94334.1. Different initiation.
    PIRiA84591.
    RefSeqiNP_565477.1. NM_127618.3.
    UniGeneiAt.22879.

    Genome annotation databases

    EnsemblPlantsiAT2G20580.1; AT2G20580.1; AT2G20580.
    GeneIDi816580.
    KEGGiath:AT2G20580.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY230828 mRNA. Translation: AAP86655.1 .
    AC007048 Genomic DNA. Translation: AAD21708.2 .
    CP002685 Genomic DNA. Translation: AEC07032.1 .
    AF360216 mRNA. Translation: AAK25926.1 .
    AY040061 mRNA. Translation: AAK64119.1 .
    AK220903 mRNA. Translation: BAD94334.1 . Different initiation.
    PIRi A84591.
    RefSeqi NP_565477.1. NM_127618.3.
    UniGenei At.22879.

    3D structure databases

    ProteinModelPortali Q9SIV2.
    SMRi Q9SIV2. Positions 50-891.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 1933. 28 interactions.
    IntActi Q9SIV2. 28 interactions.

    Proteomic databases

    PaxDbi Q9SIV2.
    PRIDEi Q9SIV2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G20580.1 ; AT2G20580.1 ; AT2G20580 .
    GeneIDi 816580.
    KEGGi ath:AT2G20580.

    Organism-specific databases

    TAIRi AT2G20580.

    Phylogenomic databases

    eggNOGi COG5110.
    HOGENOMi HOG000176022.
    InParanoidi Q9SIV2.
    KOi K03028.
    OMAi IAQGLTH.
    PhylomeDBi Q9SIV2.

    Enzyme and pathway databases

    Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_107429. Orc1 removal from chromatin.
    REACT_185297. Separation of Sister Chromatids.
    REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_187719. ER-Phagosome pathway.
    REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_190947. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Miscellaneous databases

    PROi Q9SIV2.

    Gene expression databases

    Genevestigatori Q9SIV2.

    Family and domain databases

    Gene3Di 1.25.10.10. 3 hits.
    InterProi IPR016643. 26S_Psome_Rpn1.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002015. Proteasome/cyclosome_rpt.
    [Graphical view ]
    PANTHERi PTHR10943:SF1. PTHR10943:SF1. 1 hit.
    Pfami PF01851. PC_rep. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF015965. 26S_Psome_Rpn1. 1 hit.
    SUPFAMi SSF48371. SSF48371. 4 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms."
      Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D.
      J. Biol. Chem. 279:6401-6413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 643-891.
      Strain: cv. Columbia.
    6. "The RPN1 subunit of the 26S proteasome in Arabidopsis is essential for embryogenesis."
      Brukhin V., Gheyselinck J., Gagliardini V., Genschik P., Grossniklaus U.
      Plant Cell 17:2723-2737(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    7. "The Arabidopsis 26S proteasome subunit RPN1a is required for optimal plant growth and stress responses."
      Wang S., Kurepa J., Smalle J.A.
      Plant Cell Physiol. 50:1721-1725(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    8. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
      Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
      Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
    9. "Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
      Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
      J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT, ACETYLATION AT THR-219, UBIQUITINATION AT LYS-218.
    10. "RPN1a, a 26S proteasome subunit, is required for innate immunity in Arabidopsis."
      Yao C., Wu Y., Nie H., Tang D.
      Plant J. 71:1015-1028(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INDUCTION BY SALICYLIC ACID.

    Entry informationi

    Entry nameiPSD2A_ARATH
    AccessioniPrimary (citable) accession number: Q9SIV2
    Secondary accession number(s): Q56ZR0, Q9C5J1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    The embryo lethal disruption phenotype (PubMed:16169895) could be confirmed (PubMed:19605416).2 Publications

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3