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Q9SIU8

- P2C20_ARATH

UniProt

Q9SIU8 - P2C20_ARATH

Protein

Probable protein phosphatase 2C 20

Gene

PPC3-1.2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 3 (14 Apr 2009)
      Previous versions | rss
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    Functioni

    May be involved in defense signaling.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi68 – 681Manganese 1By similarity
    Metal bindingi68 – 681Manganese 2By similarity
    Metal bindingi69 – 691Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi230 – 2301Manganese 2By similarity
    Metal bindingi269 – 2691Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G20630-MONOMER.
    ARA:GQT-353-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable protein phosphatase 2C 20 (EC:3.1.3.16)
    Short name:
    AtPP2C20
    Alternative name(s):
    AtPPC3;1.2
    Gene namesi
    Name:PPC3-1.2
    Ordered Locus Names:At2g20630
    ORF Names:F23N11.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G20630.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 290290Probable protein phosphatase 2C 20PRO_0000367950Add
    BLAST

    Proteomic databases

    PaxDbiQ9SIU8.
    PRIDEiQ9SIU8.

    Expressioni

    Inductioni

    By the avirulence factor AvrRpm1 (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriQ9SIU8.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SIU8.
    SMRiQ9SIU8. Positions 44-278.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini62 – 271210PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000233896.
    InParanoidiQ9SIU8.
    OMAiNAYISTD.
    PhylomeDBiQ9SIU8.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9SIU8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGREILHKM KVGLCGSDTG RGKTKVWKNI AHGYDFVKGK AGHPMEDYVV    50
    SEFKKVDGHD LGLFAIFDGH LGHDVAKYLQ TNLFDNILKE KDFWTDTKNA 100
    IRNAYISTDA VILEQSLKLG KGGSTAVTGI LIDGKTLVIA NVGDSRAVMS 150
    KNGVASQLSV DHEPSKEQKE IESRGGFVSN IPGDVPRVDG QLAVARAFGD 200
    KSLKIHLSSD PDIRDENIDH ETEFILFASD GVWKVMSNQE AVDLIKSIKD 250
    PQAAAKELIE EAVSKQSTDD ISCIVPCFLR REALSERYCR 290

    Note: No experimental confirmation available.

    Length:290
    Mass (Da):31,847
    Last modified:April 14, 2009 - v3
    Checksum:i622AB1EEC1D739CB
    GO
    Isoform 2 (identifier: Q9SIU8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         276-279: PCFL → VRFQ
         280-290: Missing.

    Show »
    Length:279
    Mass (Da):30,497
    Checksum:iB7B405A3D5C5D507
    GO

    Sequence cautioni

    The sequence BX842471 differs from that shown. Reason: Sequencing errors.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti39 – 391G → D in BAD43676. (PubMed:14993207)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei276 – 2794PCFL → VRFQ in isoform 2. 3 PublicationsVSP_036763
    Alternative sequencei280 – 29011Missing in isoform 2. 3 PublicationsVSP_036764Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB079672 mRNA. Translation: BAB84701.1.
    AC007048 Genomic DNA. Translation: AAD21710.2.
    CP002685 Genomic DNA. Translation: AEC07049.1.
    CP002685 Genomic DNA. Translation: AEC07050.1.
    AF411787 mRNA. Translation: AAL06477.1.
    AY093793 mRNA. Translation: AAM10409.1.
    BX842471 mRNA. No translation available.
    AK175113 mRNA. Translation: BAD42876.1.
    AK175149 mRNA. Translation: BAD42912.1.
    AK175260 mRNA. Translation: BAD43023.1.
    AK175913 mRNA. Translation: BAD43676.1.
    AK175927 mRNA. Translation: BAD43690.1.
    AK176179 mRNA. Translation: BAD43942.1.
    AK176199 mRNA. Translation: BAD43962.1.
    AK176314 mRNA. Translation: BAD44077.1.
    AK220638 mRNA. Translation: BAD95097.1.
    PIRiE84591.
    RefSeqiNP_565480.1. NM_127627.2. [Q9SIU8-2]
    NP_973490.1. NM_201761.1. [Q9SIU8-1]
    UniGeneiAt.25486.
    At.25520.
    At.49348.

    Genome annotation databases

    EnsemblPlantsiAT2G20630.2; AT2G20630.2; AT2G20630. [Q9SIU8-1]
    GeneIDi816590.
    KEGGiath:AT2G20630.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB079672 mRNA. Translation: BAB84701.1 .
    AC007048 Genomic DNA. Translation: AAD21710.2 .
    CP002685 Genomic DNA. Translation: AEC07049.1 .
    CP002685 Genomic DNA. Translation: AEC07050.1 .
    AF411787 mRNA. Translation: AAL06477.1 .
    AY093793 mRNA. Translation: AAM10409.1 .
    BX842471 mRNA. No translation available.
    AK175113 mRNA. Translation: BAD42876.1 .
    AK175149 mRNA. Translation: BAD42912.1 .
    AK175260 mRNA. Translation: BAD43023.1 .
    AK175913 mRNA. Translation: BAD43676.1 .
    AK175927 mRNA. Translation: BAD43690.1 .
    AK176179 mRNA. Translation: BAD43942.1 .
    AK176199 mRNA. Translation: BAD43962.1 .
    AK176314 mRNA. Translation: BAD44077.1 .
    AK220638 mRNA. Translation: BAD95097.1 .
    PIRi E84591.
    RefSeqi NP_565480.1. NM_127627.2. [Q9SIU8-2 ]
    NP_973490.1. NM_201761.1. [Q9SIU8-1 ]
    UniGenei At.25486.
    At.25520.
    At.49348.

    3D structure databases

    ProteinModelPortali Q9SIU8.
    SMRi Q9SIU8. Positions 44-278.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9SIU8.
    PRIDEi Q9SIU8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G20630.2 ; AT2G20630.2 ; AT2G20630 . [Q9SIU8-1 ]
    GeneIDi 816590.
    KEGGi ath:AT2G20630.

    Organism-specific databases

    TAIRi AT2G20630.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000233896.
    InParanoidi Q9SIU8.
    OMAi NAYISTD.
    PhylomeDBi Q9SIU8.

    Enzyme and pathway databases

    BioCyci ARA:AT2G20630-MONOMER.
    ARA:GQT-353-MONOMER.

    Gene expression databases

    Genevestigatori Q9SIU8.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Substrate specificity of type 2C protein phosphatases (PP2C) in Arabidopsis thaliana."
      Izumi S., Yamada M., Ohsato H., Miyazaki S., Bohnert H.J., Fukuhara T.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    8. "Combining subproteome enrichment and Rubisco depletion enables identification of low abundance proteins differentially regulated during plant defense."
      Widjaja I., Naumann K., Roth U., Wolf N., Mackey D., Dangl J.L., Scheel D., Lee J.
      Proteomics 9:138-147(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiP2C20_ARATH
    AccessioniPrimary (citable) accession number: Q9SIU8
    Secondary accession number(s): Q680F4, Q945Q0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 14, 2009
    Last modified: October 1, 2014
    This is version 92 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3