Q9SIU8 (P2C20_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 20
Short name=AtPP2C20
EC=3.1.3.16

Alternative name(s):
AtPPC3;1.2

Gene names

Name:	PPC3-1.2
Ordered Locus Names:	At2g20630
ORF Names:	F23N11.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in defense signaling.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Induction	By the avirulence factor AvrRpm1 (at protein level). Ref.8
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence BX842471 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9SIU8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q9SIU8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     276-279: PCFL → VRFQ
     280-290: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Probable protein phosphatase 2C 20		PRO_0000367950
Regions
	Domain	62 – 271	210	PP2C-like
Sites
	Metal binding	68	1	Manganese 1 By similarity
	Metal binding	68	1	Manganese 2 By similarity
	Metal binding	69	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	230	1	Manganese 2 By similarity
	Metal binding	269	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	276 – 279	4	PCFL → VRFQ in isoform 2.		VSP_036763
	Alternative sequence	280 – 290	11	Missing in isoform 2.		VSP_036764
Experimental info
	Sequence conflict	39	1	G → D in BAD43676. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 14, 2009. Version 3. Checksum: 622AB1EEC1D739CB	FASTA	290	31,847
	10 20 30 40 50 60 MAGREILHKM KVGLCGSDTG RGKTKVWKNI AHGYDFVKGK AGHPMEDYVV SEFKKVDGHD 70 80 90 100 110 120 LGLFAIFDGH LGHDVAKYLQ TNLFDNILKE KDFWTDTKNA IRNAYISTDA VILEQSLKLG 130 140 150 160 170 180 KGGSTAVTGI LIDGKTLVIA NVGDSRAVMS KNGVASQLSV DHEPSKEQKE IESRGGFVSN 190 200 210 220 230 240 IPGDVPRVDG QLAVARAFGD KSLKIHLSSD PDIRDENIDH ETEFILFASD GVWKVMSNQE 250 260 270 280 290 AVDLIKSIKD PQAAAKELIE EAVSKQSTDD ISCIVPCFLR REALSERYCR
	Isoform 2 [UniParc]. Checksum: B7B405A3D5C5D507	FASTA	279	30,497
	10 20 30 40 50 60 MAGREILHKM KVGLCGSDTG RGKTKVWKNI AHGYDFVKGK AGHPMEDYVV SEFKKVDGHD 70 80 90 100 110 120 LGLFAIFDGH LGHDVAKYLQ TNLFDNILKE KDFWTDTKNA IRNAYISTDA VILEQSLKLG 130 140 150 160 170 180 KGGSTAVTGI LIDGKTLVIA NVGDSRAVMS KNGVASQLSV DHEPSKEQKE IESRGGFVSN 190 200 210 220 230 240 IPGDVPRVDG QLAVARAFGD KSLKIHLSSD PDIRDENIDH ETEFILFASD GVWKVMSNQE 250 260 270 AVDLIKSIKD PQAAAKELIE EAVSKQSTDD ISCIVVRFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Substrate specificity of type 2C protein phosphatases (PP2C) in Arabidopsis thaliana." Izumi S., Yamada M., Ohsato H., Miyazaki S., Bohnert H.J., Fukuhara T. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[5]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[7]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Combining subproteome enrichment and Rubisco depletion enables identification of low abundance proteins differentially regulated during plant defense." Widjaja I., Naumann K., Roth U., Wolf N., Mackey D., Dangl J.L., Scheel D., Lee J. Proteomics 9:138-147(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB079672 mRNA. Translation: BAB84701.1. AC007048 Genomic DNA. Translation: AAD21710.2. CP002685 Genomic DNA. Translation: AEC07049.1. CP002685 Genomic DNA. Translation: AEC07050.1. AF411787 mRNA. Translation: AAL06477.1. AY093793 mRNA. Translation: AAM10409.1. BX842471 mRNA. No translation available. AK175113 mRNA. Translation: BAD42876.1. AK175149 mRNA. Translation: BAD42912.1. AK175260 mRNA. Translation: BAD43023.1. AK175913 mRNA. Translation: BAD43676.1. AK175927 mRNA. Translation: BAD43690.1. AK176179 mRNA. Translation: BAD43942.1. AK176199 mRNA. Translation: BAD43962.1. AK176314 mRNA. Translation: BAD44077.1. AK220638 mRNA. Translation: BAD95097.1.
IPI	IPI00545935. IPI00546580.
PIR	E84591.
RefSeq	NP_565480.1. NM_127627.2. NP_973490.1. NM_201761.1.
UniGene	At.25486. At.25520. At.49348.
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ProteinModelPortal	Q9SIU8.
SMR	Q9SIU8. Positions 60-278.
ModBase	Search...
Proteomic databases
PaxDb	Q9SIU8.
PRIDE	Q9SIU8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G20630.2; AT2G20630.2; AT2G20630.
GeneID	816590.
KEGG	ath:AT2G20630.
Organism-specific databases
TAIR	At2g20630.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
InParanoid	Q9SIU8.
OMA	IRNAYIS.
PhylomeDB	Q9SIU8.
ProtClustDB	CLSN2685102.
Gene expression databases
Genevestigator	Q9SIU8.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C20_ARATH

Accession

Primary (citable) accession number: Q9SIU8
Secondary accession number(s): Q680F4, Q945Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 14, 2009
Last modified:	May 1, 2013
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program