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Q9SIU8

- P2C20_ARATH

UniProt

Q9SIU8 - P2C20_ARATH

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Protein

Probable protein phosphatase 2C 20

Gene
PPC3-1.2, At2g20630, F23N11.5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

May be involved in defense signaling.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi68 – 681Manganese 1 By similarity
Metal bindingi68 – 681Manganese 2 By similarity
Metal bindingi69 – 691Manganese 1; via carbonyl oxygen By similarity
Metal bindingi230 – 2301Manganese 2 By similarity
Metal bindingi269 – 2691Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G20630-MONOMER.
ARA:GQT-353-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 20 (EC:3.1.3.16)
Short name:
AtPP2C20
Alternative name(s):
AtPPC3;1.2
Gene namesi
Name:PPC3-1.2
Ordered Locus Names:At2g20630
ORF Names:F23N11.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G20630.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290Probable protein phosphatase 2C 20PRO_0000367950Add
BLAST

Proteomic databases

PaxDbiQ9SIU8.
PRIDEiQ9SIU8.

Expressioni

Inductioni

By the avirulence factor AvrRpm1 (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ9SIU8.

Structurei

3D structure databases

ProteinModelPortaliQ9SIU8.
SMRiQ9SIU8. Positions 44-278.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 271210PP2C-likeAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.
Contains 1 PP2C-like domain.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9SIU8.
OMAiNAYISTD.
PhylomeDBiQ9SIU8.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SIU8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAGREILHKM KVGLCGSDTG RGKTKVWKNI AHGYDFVKGK AGHPMEDYVV    50
SEFKKVDGHD LGLFAIFDGH LGHDVAKYLQ TNLFDNILKE KDFWTDTKNA 100
IRNAYISTDA VILEQSLKLG KGGSTAVTGI LIDGKTLVIA NVGDSRAVMS 150
KNGVASQLSV DHEPSKEQKE IESRGGFVSN IPGDVPRVDG QLAVARAFGD 200
KSLKIHLSSD PDIRDENIDH ETEFILFASD GVWKVMSNQE AVDLIKSIKD 250
PQAAAKELIE EAVSKQSTDD ISCIVPCFLR REALSERYCR 290

Note: No experimental confirmation available.

Length:290
Mass (Da):31,847
Last modified:April 14, 2009 - v3
Checksum:i622AB1EEC1D739CB
GO
Isoform 2 (identifier: Q9SIU8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     276-279: PCFL → VRFQ
     280-290: Missing.

Show »
Length:279
Mass (Da):30,497
Checksum:iB7B405A3D5C5D507
GO

Sequence cautioni

The sequence BX842471 differs from that shown. Reason: Sequencing errors.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei276 – 2794PCFL → VRFQ in isoform 2. VSP_036763
Alternative sequencei280 – 29011Missing in isoform 2. VSP_036764Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391G → D in BAD43676. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB079672 mRNA. Translation: BAB84701.1.
AC007048 Genomic DNA. Translation: AAD21710.2.
CP002685 Genomic DNA. Translation: AEC07049.1.
CP002685 Genomic DNA. Translation: AEC07050.1.
AF411787 mRNA. Translation: AAL06477.1.
AY093793 mRNA. Translation: AAM10409.1.
BX842471 mRNA. No translation available.
AK175113 mRNA. Translation: BAD42876.1.
AK175149 mRNA. Translation: BAD42912.1.
AK175260 mRNA. Translation: BAD43023.1.
AK175913 mRNA. Translation: BAD43676.1.
AK175927 mRNA. Translation: BAD43690.1.
AK176179 mRNA. Translation: BAD43942.1.
AK176199 mRNA. Translation: BAD43962.1.
AK176314 mRNA. Translation: BAD44077.1.
AK220638 mRNA. Translation: BAD95097.1.
PIRiE84591.
RefSeqiNP_565480.1. NM_127627.2. [Q9SIU8-2]
NP_973490.1. NM_201761.1. [Q9SIU8-1]
UniGeneiAt.25486.
At.25520.
At.49348.

Genome annotation databases

EnsemblPlantsiAT2G20630.2; AT2G20630.2; AT2G20630. [Q9SIU8-1]
GeneIDi816590.
KEGGiath:AT2G20630.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB079672 mRNA. Translation: BAB84701.1 .
AC007048 Genomic DNA. Translation: AAD21710.2 .
CP002685 Genomic DNA. Translation: AEC07049.1 .
CP002685 Genomic DNA. Translation: AEC07050.1 .
AF411787 mRNA. Translation: AAL06477.1 .
AY093793 mRNA. Translation: AAM10409.1 .
BX842471 mRNA. No translation available.
AK175113 mRNA. Translation: BAD42876.1 .
AK175149 mRNA. Translation: BAD42912.1 .
AK175260 mRNA. Translation: BAD43023.1 .
AK175913 mRNA. Translation: BAD43676.1 .
AK175927 mRNA. Translation: BAD43690.1 .
AK176179 mRNA. Translation: BAD43942.1 .
AK176199 mRNA. Translation: BAD43962.1 .
AK176314 mRNA. Translation: BAD44077.1 .
AK220638 mRNA. Translation: BAD95097.1 .
PIRi E84591.
RefSeqi NP_565480.1. NM_127627.2. [Q9SIU8-2 ]
NP_973490.1. NM_201761.1. [Q9SIU8-1 ]
UniGenei At.25486.
At.25520.
At.49348.

3D structure databases

ProteinModelPortali Q9SIU8.
SMRi Q9SIU8. Positions 44-278.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9SIU8.
PRIDEi Q9SIU8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G20630.2 ; AT2G20630.2 ; AT2G20630 . [Q9SIU8-1 ]
GeneIDi 816590.
KEGGi ath:AT2G20630.

Organism-specific databases

TAIRi AT2G20630.

Phylogenomic databases

eggNOGi COG0631.
HOGENOMi HOG000233896.
InParanoidi Q9SIU8.
OMAi NAYISTD.
PhylomeDBi Q9SIU8.

Enzyme and pathway databases

BioCyci ARA:AT2G20630-MONOMER.
ARA:GQT-353-MONOMER.

Gene expression databases

Genevestigatori Q9SIU8.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Substrate specificity of type 2C protein phosphatases (PP2C) in Arabidopsis thaliana."
    Izumi S., Yamada M., Ohsato H., Miyazaki S., Bohnert H.J., Fukuhara T.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Combining subproteome enrichment and Rubisco depletion enables identification of low abundance proteins differentially regulated during plant defense."
    Widjaja I., Naumann K., Roth U., Wolf N., Mackey D., Dangl J.L., Scheel D., Lee J.
    Proteomics 9:138-147(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiP2C20_ARATH
AccessioniPrimary (citable) accession number: Q9SIU8
Secondary accession number(s): Q680F4, Q945Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: May 14, 2014
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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