Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9SIU8 (P2C20_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable protein phosphatase 2C 20

Short name=AtPP2C20
EC=3.1.3.16
Alternative name(s):
AtPPC3;1.2
Gene names
Name:PPC3-1.2
Ordered Locus Names:At2g20630
ORF Names:F23N11.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in defense signaling.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Induction

By the avirulence factor AvrRpm1 (at protein level). Ref.8

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence caution

The sequence BX842471 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9SIU8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: Q9SIU8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     276-279: PCFL → VRFQ
     280-290: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290Probable protein phosphatase 2C 20
PRO_0000367950

Regions

Domain62 – 271210PP2C-like

Sites

Metal binding681Manganese 1 By similarity
Metal binding681Manganese 2 By similarity
Metal binding691Manganese 1; via carbonyl oxygen By similarity
Metal binding2301Manganese 2 By similarity
Metal binding2691Manganese 2 By similarity

Natural variations

Alternative sequence276 – 2794PCFL → VRFQ in isoform 2.
VSP_036763
Alternative sequence280 – 29011Missing in isoform 2.
VSP_036764

Experimental info

Sequence conflict391G → D in BAD43676. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 14, 2009. Version 3.
Checksum: 622AB1EEC1D739CB

FASTA29031,847
        10         20         30         40         50         60 
MAGREILHKM KVGLCGSDTG RGKTKVWKNI AHGYDFVKGK AGHPMEDYVV SEFKKVDGHD 

        70         80         90        100        110        120 
LGLFAIFDGH LGHDVAKYLQ TNLFDNILKE KDFWTDTKNA IRNAYISTDA VILEQSLKLG 

       130        140        150        160        170        180 
KGGSTAVTGI LIDGKTLVIA NVGDSRAVMS KNGVASQLSV DHEPSKEQKE IESRGGFVSN 

       190        200        210        220        230        240 
IPGDVPRVDG QLAVARAFGD KSLKIHLSSD PDIRDENIDH ETEFILFASD GVWKVMSNQE 

       250        260        270        280        290 
AVDLIKSIKD PQAAAKELIE EAVSKQSTDD ISCIVPCFLR REALSERYCR 

« Hide

Isoform 2 [UniParc].

Checksum: B7B405A3D5C5D507
Show »

FASTA27930,497

References

« Hide 'large scale' references
[1]"Substrate specificity of type 2C protein phosphatases (PP2C) in Arabidopsis thaliana."
Izumi S., Yamada M., Ohsato H., Miyazaki S., Bohnert H.J., Fukuhara T.
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[5]"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[7]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[8]"Combining subproteome enrichment and Rubisco depletion enables identification of low abundance proteins differentially regulated during plant defense."
Widjaja I., Naumann K., Roth U., Wolf N., Mackey D., Dangl J.L., Scheel D., Lee J.
Proteomics 9:138-147(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB079672 mRNA. Translation: BAB84701.1.
AC007048 Genomic DNA. Translation: AAD21710.2.
CP002685 Genomic DNA. Translation: AEC07049.1.
CP002685 Genomic DNA. Translation: AEC07050.1.
AF411787 mRNA. Translation: AAL06477.1.
AY093793 mRNA. Translation: AAM10409.1.
BX842471 mRNA. No translation available.
AK175113 mRNA. Translation: BAD42876.1.
AK175149 mRNA. Translation: BAD42912.1.
AK175260 mRNA. Translation: BAD43023.1.
AK175913 mRNA. Translation: BAD43676.1.
AK175927 mRNA. Translation: BAD43690.1.
AK176179 mRNA. Translation: BAD43942.1.
AK176199 mRNA. Translation: BAD43962.1.
AK176314 mRNA. Translation: BAD44077.1.
AK220638 mRNA. Translation: BAD95097.1.
PIRE84591.
RefSeqNP_565480.1. NM_127627.2. [Q9SIU8-2]
NP_973490.1. NM_201761.1. [Q9SIU8-1]
UniGeneAt.25486.
At.25520.
At.49348.

3D structure databases

ProteinModelPortalQ9SIU8.
SMRQ9SIU8. Positions 44-278.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9SIU8.
PRIDEQ9SIU8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G20630.2; AT2G20630.2; AT2G20630. [Q9SIU8-1]
GeneID816590.
KEGGath:AT2G20630.

Organism-specific databases

TAIRAT2G20630.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000233896.
InParanoidQ9SIU8.
OMANAYISTD.
PhylomeDBQ9SIU8.

Enzyme and pathway databases

BioCycARA:AT2G20630-MONOMER.
ARA:GQT-353-MONOMER.

Gene expression databases

GenevestigatorQ9SIU8.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
ProtoNetSearch...

Entry information

Entry nameP2C20_ARATH
AccessionPrimary (citable) accession number: Q9SIU8
Secondary accession number(s): Q680F4, Q945Q0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: May 14, 2014
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names