Q9SIJ9 (PME11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative pectinesterase 11
Short name=PE 11
EC=3.1.1.11

Alternative name(s):
Pectin methylesterase 11
Short name=AtPME11

Gene names

Name:	PME11
Synonyms:	ARATH11
Ordered Locus Names:	At2g21610
ORF Names:	F2G1.12

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl esterase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Putative pectinesterase 11		PRO_0000371668
Regions
	Transmembrane	13 – 35	23	Helical; Potential
	Compositional bias	36 – 42	7	Poly-Ser
Sites
	Active site	175	1	Proton donor By similarity
	Active site	196	1	Nucleophile By similarity
	Binding site	252	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity
	Site	174	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	76	1	N-linked (GlcNAc...) Potential
	Glycosylation	218	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9SIJ9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B166A3E21A4A525D	FASTA	352	39,058
	10 20 30 40 50 60 MGLYKTKSKR SIANYHHIII INIFILSSIT SSSMASSSSP SSIDFSTAIL IRVDQSGKGD 70 80 90 100 110 120 FSKIQEAIES IPPNLNNSQL YFIWVKPGIY REKVVIPAEK PYITLSGTQA SNTFLIWSDG 130 140 150 160 170 180 EDILESPTLT IFASDFVCRF LTIQNKFGTA GRAVALRVAA DKAAFYGCVI TSYQDTLLDD 190 200 210 220 230 240 NGNHYFKNCY IEGATDFICG SASSLYERCH LHSLSPNNGS ITAQMRTSAT EKSGFTFLGC 250 260 270 280 290 300 KLTGSGSTFL GRPWGAYSRV VFAYSFFSNV VAPQGWNQWG DSTKENTVYY GEYKCYGPGA 310 320 330 340 350 DREQRVEWSK QLSDEEATVF LSKDFIGGKD WLRPAPSHFK NAPKQTQNKE IN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007119 Genomic DNA. Translation: AAD23644.1. CP002685 Genomic DNA. Translation: AEC07202.1.
IPI	IPI00529664.
PIR	C84603.
RefSeq	NP_179755.1. NM_127732.1.
UniGene	At.52856.
3D structure databases
HSSP	HSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ProteinModelPortal	Q9SIJ9.
SMR	Q9SIJ9. Positions 49-341.
ModBase	Search...
Proteomic databases
PRIDE	Q9SIJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G21610.1; AT2G21610.1; AT2G21610.
GeneID	816699.
GenomeReviews	Gene locus AT2G21610 in contig CT485783_GR.
KEGG	ath:AT2G21610.
NMPDR	fig|3702.1.peg.9185.
Organism-specific databases
GeneFarm	429. 8.
TAIR	At2g21610.
Phylogenomic databases
eggNOG	euNOG09608.
GeneTree	EPGT00070000028060.
HOGENOM	HBG747179.
InParanoid	Q9SIJ9.
OMA	PWGPYSR.
PhylomeDB	Q9SIJ9.
ProtClustDB	PLN02432.
Gene expression databases
ArrayExpress	Q9SIJ9.
Genevestigator	Q9SIJ9.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME11_ARATH

Accession

Primary (citable) accession number: Q9SIJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program