Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin domain-containing protein DSK2a

Gene

DSK2A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds and presumably selects ubiquitin-conjugates for destruction. Prefers multiubiquitin chains rather than single ubiquitins, with a binding affinity for 'Lys-48'-linked ubiquitin chains. Acts as a ubiquitin receptor that associates with the 26S proteasomal docking subunit RPN10 for the indirect recognition of ubiquitinated substrates of ubiquitin/26S proteasome-mediated proteolysis (UPP).2 Publications

GO - Molecular functioni

  • polyubiquitin modification-dependent protein binding Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-ATH-8856825 Cargo recognition for clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin domain-containing protein DSK2a
Gene namesi
Name:DSK2A
Ordered Locus Names:At2g17190
ORF Names:T23A1.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G17190
TAIRilocus:2059677 AT2G17190

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61I → A: Abolishes interaction with RPN13. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004231791 – 538Ubiquitin domain-containing protein DSK2aAdd BLAST538

Proteomic databases

PaxDbiQ9SII9

Expressioni

Tissue specificityi

Ubiquitous with a strong expression level in inflorescence.1 Publication

Gene expression databases

ExpressionAtlasiQ9SII9 baseline and differential
GenevisibleiQ9SII9 AT

Interactioni

Subunit structurei

Interacts with 'Lys-48'-linked polyubiquitin chains via its UBA domain. Interacts with RPN10 and RPN13. Interacts with PEX2 and PEX12.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PEX2Q9CA863EBI-6860633,EBI-4470254

GO - Molecular functioni

  • polyubiquitin modification-dependent protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi15813 interactors.
IntActiQ9SII9 3 interactors.
STRINGi3702.AT2G17190.1

Structurei

3D structure databases

ProteinModelPortaliQ9SII9
SMRiQ9SII9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 93Ubiquitin-likePROSITE-ProRule annotationAdd BLAST76
Domaini138 – 179STI1 1Add BLAST42
Domaini192 – 231STI1 2Add BLAST40
Domaini357 – 394STI1 3Add BLAST38
Domaini398 – 433STI1 4Add BLAST36
Domaini491 – 535UBAPROSITE-ProRule annotationAdd BLAST45

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0010 Eukaryota
COG5272 LUCA
HOGENOMiHOG000234878
InParanoidiQ9SII9
KOiK04523
OMAiSSFMEMQ
OrthoDBiEOG09360AUZ
PhylomeDBiQ9SII9

Family and domain databases

InterProiView protein in InterPro
IPR006636 STI1_HS-bd
IPR015940 UBA
IPR009060 UBA-like_sf
IPR015496 Ubiquilin
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
PANTHERiPTHR10677 PTHR10677, 1 hit
PfamiView protein in Pfam
PF00627 UBA, 1 hit
PF00240 ubiquitin, 1 hit
SMARTiView protein in SMART
SM00727 STI1, 4 hits
SM00165 UBA, 1 hit
SM00213 UBQ, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 1 hit
PS50053 UBIQUITIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SII9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGEADSRQP LTAEGVAVAV NVRCSNGTKF SVTTSLDSTV ESFKELIAQN
60 70 80 90 100
SDVPANQQRL IYKGRILKDD QTLLSYGLQA DHTVHMVRGF VPSSPSAPAA
110 120 130 140 150
NAGNQTTAPQ AVGSNDSSNL GGGESLFPGL GFNPLGGGNA MAGLFGSGLP
160 170 180 190 200
DLEQAQQQLA QNPNMIREMM NTPAIQNLMN NPEFMRSMIM NNPQMRELVD
210 220 230 240 250
RNPELGHVLN DPSILRQTLE AARNPELMRE MMRNTDRAMS NIESMPEGFN
260 270 280 290 300
MLRRMYENVQ EPLMNATTMS ENAGNNTSSN PFAALLGNQG VTTQGSDTSN
310 320 330 340 350
NISAPNAETG TPNANPLPNP WGATAGQTTA PGRTNAGLGG LGGLGGLGGL
360 370 380 390 400
GMLGADSPLG ATPDASQLSQ ILQNPAMSQM MQSVLSNPQY MNQLMSLNPQ
410 420 430 440 450
LRSMLDMNPQ LREMMQNPDF LRQFSSPEMM QQMMSLQQSL FSQNRNTAGQ
460 470 480 490 500
DPTQTGAATG TANNGGLDLL MNMFGSLGAG GLSGTNQPNV PPEERFATQL
510 520 530
QQLQEMGFYD RAENIRALLA TNGNVNAAVE RLLGSIGQ
Length:538
Mass (Da):57,285
Last modified:June 1, 2002 - v2
Checksum:iAF2BF2BF36413DBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007127 Genomic DNA Translation: AAD25137.2
CP002685 Genomic DNA Translation: AEC06596.1
AF360159 mRNA Translation: AAK25869.1
AY042828 mRNA Translation: AAK68768.1
AY081450 mRNA Translation: AAM10012.1
AY113886 mRNA Translation: AAM44934.1
AK228616 mRNA Translation: BAF00527.1
PIRiB84549
RefSeqiNP_565407.1, NM_127273.4
UniGeneiAt.21851

Genome annotation databases

EnsemblPlantsiAT2G17190.1; AT2G17190.1; AT2G17190
GeneIDi816224
GrameneiAT2G17190.1; AT2G17190.1; AT2G17190
KEGGiath:AT2G17190

Similar proteinsi

Entry informationi

Entry nameiDSK2A_ARATH
AccessioniPrimary (citable) accession number: Q9SII9
Secondary accession number(s): Q9C5L7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome