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Protein

Hydroxyacylglutathione hydrolase 2, mitochondrial

Gene

At2g31350

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.1 Publication

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (GLX1), Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (At1g11840), Lactoylglutathione lyase (F12F1.32), Lactoylglutathione lyase (GLX1), Lactoylglutathione lyase (AXX17_At1g07880), Probable lactoylglutathione lyase, chloroplastic (At1g67280), Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (At1g11840), Lactoylglutathione lyase (At1g08110)
  2. Hydroxyacylglutathione hydrolase cytoplasmic (GLX2-2), Hydroxyacylglutathione hydrolase 2, mitochondrial (At2g31350), Probable hydroxyacylglutathione hydrolase 2, chloroplastic (GLX2-4), Hydroxyacylglutathione hydrolase 1, mitochondrial (GLX2-1)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi124Zinc2 Publications1
Metal bindingi126Zinc2 Publications1
Metal bindingi128Iron2 Publications1
Metal bindingi129Iron2 Publications1
Metal bindingi182Zinc2 Publications1
Metal bindingi201Iron2 Publications1
Metal bindingi201Zinc2 Publications1
Metal bindingi239Iron2 Publications1

GO - Molecular functioni

  • hydroxyacylglutathione hydrolase activity Source: TAIR
  • iron ion binding Source: TAIR
  • zinc ion binding Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.2.6. 399.
UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase 2, mitochondrial (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Ordered Locus Names:At2g31350
ORF Names:T28P16.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G31350.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 64MitochondrionSequence analysisAdd BLAST64
ChainiPRO_000001228765 – 324Hydroxyacylglutathione hydrolase 2, mitochondrialAdd BLAST260

Proteomic databases

PaxDbiQ9SID3.

Expressioni

Gene expression databases

GenevisibleiQ9SID3. AT.

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

STRINGi3702.AT2G31350.1.

Structurei

Secondary structure

1324
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi72 – 78Combined sources7
Turni79 – 81Combined sources3
Beta strandi82 – 88Combined sources7
Turni90 – 92Combined sources3
Beta strandi95 – 98Combined sources4
Helixi103 – 113Combined sources11
Beta strandi119 – 121Combined sources3
Helixi127 – 130Combined sources4
Helixi133 – 140Combined sources8
Beta strandi143 – 147Combined sources5
Helixi148 – 153Combined sources6
Beta strandi157 – 161Combined sources5
Beta strandi166 – 169Combined sources4
Beta strandi172 – 178Combined sources7
Beta strandi181 – 185Combined sources5
Beta strandi187 – 191Combined sources5
Helixi192 – 194Combined sources3
Beta strandi196 – 200Combined sources5
Beta strandi212 – 214Combined sources3
Helixi216 – 227Combined sources12
Beta strandi234 – 239Combined sources6
Helixi242 – 252Combined sources11
Helixi257 – 271Combined sources15
Helixi281 – 287Combined sources7
Helixi289 – 291Combined sources3
Helixi296 – 302Combined sources7
Helixi310 – 322Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XM8X-ray1.74A/B72-324[»]
2Q42X-ray1.74A/B72-324[»]
ProteinModelPortaliQ9SID3.
SMRiQ9SID3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9SID3.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IU4V. Eukaryota.
ENOG4111FEZ. LUCA.
HOGENOMiHOG000058041.
InParanoidiQ9SID3.
KOiK01069.
OMAiLRVDQFC.
OrthoDBiEOG09360H08.
PhylomeDBiQ9SID3.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SID3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQTISKASSA TSFFRCSRKL SSQPCVRQLN IRKSLVCRVM KLVSSPLRTL
60 70 80 90 100
RGAGKSIRVS KFCSVSNVSS LQIELVPCLK DNYAYILHDE DTGTVGVVDP
110 120 130 140 150
SEAEPIIDSL KRSGRNLTYI LNTHHHYDHT GGNLELKDRY GAKVIGSAMD
160 170 180 190 200
KDRIPGIDMA LKDGDKWMFA GHEVHVMDTP GHTKGHISLY FPGSRAIFTG
210 220 230 240 250
DTMFSLSCGK LFEGTPKQML ASLQKITSLP DDTSIYCGHE YTLSNSKFAL
260 270 280 290 300
SLEPNNEVLQ SYAAHVAELR SKKLPTIPTT VKMEKACNPF LRSSNTDIRR
310 320
ALRIPEAADE AEALGIIRKA KDDF
Length:324
Mass (Da):35,841
Last modified:May 1, 2000 - v1
Checksum:i91F47F391EFB0AE1
GO
Isoform 2 (identifier: Q9SID3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     19-19: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:323
Mass (Da):35,713
Checksum:iC32FD27BAC63BCC0
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01809219Missing in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007169 Genomic DNA. Translation: AAD26483.1.
CP002685 Genomic DNA. Translation: AEC08531.1.
CP002685 Genomic DNA. Translation: AEC08532.1.
AY072200 mRNA. Translation: AAL60021.1.
AY096672 mRNA. Translation: AAM20306.1.
PIRiF84719.
RefSeqiNP_180693.1. NM_128692.5. [Q9SID3-1]
NP_850166.1. NM_179835.1. [Q9SID3-2]
UniGeneiAt.27616.

Genome annotation databases

EnsemblPlantsiAT2G31350.1; AT2G31350.1; AT2G31350. [Q9SID3-1]
GeneIDi817693.
GrameneiAT2G31350.1; AT2G31350.1; AT2G31350.
KEGGiath:AT2G31350.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007169 Genomic DNA. Translation: AAD26483.1.
CP002685 Genomic DNA. Translation: AEC08531.1.
CP002685 Genomic DNA. Translation: AEC08532.1.
AY072200 mRNA. Translation: AAL60021.1.
AY096672 mRNA. Translation: AAM20306.1.
PIRiF84719.
RefSeqiNP_180693.1. NM_128692.5. [Q9SID3-1]
NP_850166.1. NM_179835.1. [Q9SID3-2]
UniGeneiAt.27616.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XM8X-ray1.74A/B72-324[»]
2Q42X-ray1.74A/B72-324[»]
ProteinModelPortaliQ9SID3.
SMRiQ9SID3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G31350.1.

Proteomic databases

PaxDbiQ9SID3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G31350.1; AT2G31350.1; AT2G31350. [Q9SID3-1]
GeneIDi817693.
GrameneiAT2G31350.1; AT2G31350.1; AT2G31350.
KEGGiath:AT2G31350.

Organism-specific databases

TAIRiAT2G31350.

Phylogenomic databases

eggNOGiENOG410IU4V. Eukaryota.
ENOG4111FEZ. LUCA.
HOGENOMiHOG000058041.
InParanoidiQ9SID3.
KOiK01069.
OMAiLRVDQFC.
OrthoDBiEOG09360H08.
PhylomeDBiQ9SID3.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.
BRENDAi3.1.2.6. 399.

Miscellaneous databases

EvolutionaryTraceiQ9SID3.
PROiQ9SID3.

Gene expression databases

GenevisibleiQ9SID3. AT.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLO2N_ARATH
AccessioniPrimary (citable) accession number: Q9SID3
Secondary accession number(s): Q3EBQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.