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Protein

Hydroxyacylglutathione hydrolase 2, mitochondrial

Gene

At2g31350

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.1 Publication

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (GLX1), Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (At1g11840), Lactoylglutathione lyase (F12F1.32), Lactoylglutathione lyase (GLX1), Probable lactoylglutathione lyase, chloroplastic (At1g67280), Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (At1g11840), Lactoylglutathione lyase (At1g08110)
  2. Hydroxyacylglutathione hydrolase cytoplasmic (GLX2-2), Hydroxyacylglutathione hydrolase 2, mitochondrial (At2g31350), Probable hydroxyacylglutathione hydrolase 2, chloroplastic (GLX2-4), Hydroxyacylglutathione hydrolase 1, mitochondrial (GLX2-1)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi124 – 1241Zinc2 Publications
Metal bindingi126 – 1261Zinc2 Publications
Metal bindingi128 – 1281Iron2 Publications
Metal bindingi129 – 1291Iron2 Publications
Metal bindingi182 – 1821Zinc2 Publications
Metal bindingi201 – 2011Iron2 Publications
Metal bindingi201 – 2011Zinc2 Publications
Metal bindingi239 – 2391Iron2 Publications

GO - Molecular functioni

  • hydroxyacylglutathione hydrolase activity Source: TAIR
  • iron ion binding Source: TAIR
  • zinc ion binding Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:GQT-2488-MONOMER.
BRENDAi3.1.2.6. 399.
UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase 2, mitochondrial (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Ordered Locus Names:At2g31350
ORF Names:T28P16.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G31350.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6464MitochondrionSequence analysisAdd
BLAST
Chaini65 – 324260Hydroxyacylglutathione hydrolase 2, mitochondrialPRO_0000012287Add
BLAST

Proteomic databases

PaxDbiQ9SID3.
PRIDEiQ9SID3.

Expressioni

Gene expression databases

GenevisibleiQ9SID3. AT.

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

STRINGi3702.AT2G31350.1.

Structurei

Secondary structure

1
324
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi72 – 787Combined sources
Turni79 – 813Combined sources
Beta strandi82 – 887Combined sources
Turni90 – 923Combined sources
Beta strandi95 – 984Combined sources
Helixi103 – 11311Combined sources
Beta strandi119 – 1213Combined sources
Helixi127 – 1304Combined sources
Helixi133 – 1408Combined sources
Beta strandi143 – 1475Combined sources
Helixi148 – 1536Combined sources
Beta strandi157 – 1615Combined sources
Beta strandi166 – 1694Combined sources
Beta strandi172 – 1787Combined sources
Beta strandi181 – 1855Combined sources
Beta strandi187 – 1915Combined sources
Helixi192 – 1943Combined sources
Beta strandi196 – 2005Combined sources
Beta strandi212 – 2143Combined sources
Helixi216 – 22712Combined sources
Beta strandi234 – 2396Combined sources
Helixi242 – 25211Combined sources
Helixi257 – 27115Combined sources
Helixi281 – 2877Combined sources
Helixi289 – 2913Combined sources
Helixi296 – 3027Combined sources
Helixi310 – 32213Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XM8X-ray1.74A/B72-324[»]
2Q42X-ray1.74A/B72-324[»]
ProteinModelPortaliQ9SID3.
SMRiQ9SID3. Positions 71-324.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9SID3.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IU4V. Eukaryota.
ENOG4111FEZ. LUCA.
HOGENOMiHOG000058041.
InParanoidiQ9SID3.
KOiK01069.
OMAiLRVDQFC.
OrthoDBiEOG09360H08.
PhylomeDBiQ9SID3.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SID3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQTISKASSA TSFFRCSRKL SSQPCVRQLN IRKSLVCRVM KLVSSPLRTL
60 70 80 90 100
RGAGKSIRVS KFCSVSNVSS LQIELVPCLK DNYAYILHDE DTGTVGVVDP
110 120 130 140 150
SEAEPIIDSL KRSGRNLTYI LNTHHHYDHT GGNLELKDRY GAKVIGSAMD
160 170 180 190 200
KDRIPGIDMA LKDGDKWMFA GHEVHVMDTP GHTKGHISLY FPGSRAIFTG
210 220 230 240 250
DTMFSLSCGK LFEGTPKQML ASLQKITSLP DDTSIYCGHE YTLSNSKFAL
260 270 280 290 300
SLEPNNEVLQ SYAAHVAELR SKKLPTIPTT VKMEKACNPF LRSSNTDIRR
310 320
ALRIPEAADE AEALGIIRKA KDDF
Length:324
Mass (Da):35,841
Last modified:May 1, 2000 - v1
Checksum:i91F47F391EFB0AE1
GO
Isoform 2 (identifier: Q9SID3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     19-19: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:323
Mass (Da):35,713
Checksum:iC32FD27BAC63BCC0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei19 – 191Missing in isoform 2. CuratedVSP_018092

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007169 Genomic DNA. Translation: AAD26483.1.
CP002685 Genomic DNA. Translation: AEC08531.1.
CP002685 Genomic DNA. Translation: AEC08532.1.
AY072200 mRNA. Translation: AAL60021.1.
AY096672 mRNA. Translation: AAM20306.1.
PIRiF84719.
RefSeqiNP_180693.1. NM_128692.4. [Q9SID3-1]
NP_850166.1. NM_179835.1. [Q9SID3-2]
UniGeneiAt.27616.

Genome annotation databases

EnsemblPlantsiAT2G31350.1; AT2G31350.1; AT2G31350. [Q9SID3-1]
GeneIDi817693.
KEGGiath:AT2G31350.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007169 Genomic DNA. Translation: AAD26483.1.
CP002685 Genomic DNA. Translation: AEC08531.1.
CP002685 Genomic DNA. Translation: AEC08532.1.
AY072200 mRNA. Translation: AAL60021.1.
AY096672 mRNA. Translation: AAM20306.1.
PIRiF84719.
RefSeqiNP_180693.1. NM_128692.4. [Q9SID3-1]
NP_850166.1. NM_179835.1. [Q9SID3-2]
UniGeneiAt.27616.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XM8X-ray1.74A/B72-324[»]
2Q42X-ray1.74A/B72-324[»]
ProteinModelPortaliQ9SID3.
SMRiQ9SID3. Positions 71-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G31350.1.

Proteomic databases

PaxDbiQ9SID3.
PRIDEiQ9SID3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G31350.1; AT2G31350.1; AT2G31350. [Q9SID3-1]
GeneIDi817693.
KEGGiath:AT2G31350.

Organism-specific databases

TAIRiAT2G31350.

Phylogenomic databases

eggNOGiENOG410IU4V. Eukaryota.
ENOG4111FEZ. LUCA.
HOGENOMiHOG000058041.
InParanoidiQ9SID3.
KOiK01069.
OMAiLRVDQFC.
OrthoDBiEOG09360H08.
PhylomeDBiQ9SID3.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.
BioCyciARA:GQT-2488-MONOMER.
BRENDAi3.1.2.6. 399.

Miscellaneous databases

EvolutionaryTraceiQ9SID3.
PROiQ9SID3.

Gene expression databases

GenevisibleiQ9SID3. AT.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLO2N_ARATH
AccessioniPrimary (citable) accession number: Q9SID3
Secondary accession number(s): Q3EBQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.