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Protein

3-ketoacyl-CoA synthase 12

Gene

KCS12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A very-long-chain acyl-CoA + malonyl-CoA = CoA + a very-long-chain 3-oxoacyl-CoA + CO2.Curated

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei168By similarity1
Active sitei247By similarity1
Active sitei344By similarity1
Active sitei348By similarity1
Active sitei377By similarity1
Active sitei381By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G28630-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA synthase 121 Publication (EC:2.3.1.199Curated)
Short name:
KCS-121 Publication
Alternative name(s):
Very long-chain fatty acid condensing enzyme 121 Publication
Short name:
VLCFA condensing enzyme 121 Publication
Gene namesi
Name:KCS121 Publication
Ordered Locus Names:At2g28630Imported
ORF Names:T8O18.8Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G28630.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000024910426 – 4763-ketoacyl-CoA synthase 12Add BLAST451

Proteomic databases

PaxDbiQ9SIB2.

Expressioni

Tissue specificityi

Expressed in siliques, flowers and leaves.1 Publication

Inductioni

Repressed by herbicides such as flufenacet and benfuresate (PubMed:12916765). Up-regulated by osmotic stress and down-regulated by low temperature, salt and drought (PubMed:18465198).2 Publications

Gene expression databases

GenevisibleiQ9SIB2. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G28630.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SIB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 313FAESequence analysisAdd BLAST288

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated
Contains 1 FAE (fatty acid elongase) domain.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IH8S. Eukaryota.
ENOG410Y9JS. LUCA.
HOGENOMiHOG000238893.
InParanoidiQ9SIB2.
KOiK15397.
OMAiIAPNWYA.
OrthoDBiEOG093608SJ.
PhylomeDBiQ9SIB2.

Family and domain databases

Gene3Di3.40.47.10. 3 hits.
InterProiIPR012392. 3-ktacl-CoA_syn.
IPR013747. ACP_syn_III_C.
IPR013601. FAE1_typ3_polyketide_synth.
IPR016039. Thiolase-like.
[Graphical view]
PANTHERiPTHR31561. PTHR31561. 1 hit.
PfamiPF08541. ACP_syn_III_C. 1 hit.
PF08392. FAE1_CUT1_RppA. 1 hit.
[Graphical view]
PIRSFiPIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SIB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLLFLFFSL LLSYLFFKIW KLIDSKQDKD CYILDYQCHK PTDDRMVSTQ
60 70 80 90 100
FSGEIIYRNQ NLGLTEYKFL LKAIVSSGIG EQTYAPRLVF EGREERPSLQ
110 120 130 140 150
DGISEMEEFY VDSIGKLLER NQISPKDIDI LVVNVSMLSS TPSLASRIIN
160 170 180 190 200
HYKMRDDVKV FNLTGMGCSA SLISVDIVKN IFKSYANKLA LVATSESLSP
210 220 230 240 250
NWYSGNNRSM ILANCLFRSG GCAILLTNKR SLRKKAMFKL KCMVRTHHGA
260 270 280 290 300
REESYNCCIQ AEDEQGRVGF YLGKNLPKAA TRAFVENLKV ITPKILPVTE
310 320 330 340 350
LIRFMLKLLI KKIKIRQNPS KGSTNLPPGT PLKAGINFKT GIEHFCIHTG
360 370 380 390 400
GKAVIDGIGH SLDLNEYDIE PARMTLHRFG NTSASSLWYV LAYMEAKKRL
410 420 430 440 450
KRGDRVFMIS FGAGFKCNSC VWEVVRDLTG GESKGNVWNH CIDDYPPKSI
460 470
LNPYLEKFGW IQDEDPDTFK VPDAFM
Length:476
Mass (Da):53,974
Last modified:May 1, 2000 - v1
Checksum:i04C9124611E75D87
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti286E → D in AAM61290 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007171 Genomic DNA. Translation: AAD24372.1.
CP002685 Genomic DNA. Translation: AEC08152.1.
AY056170 mRNA. Translation: AAL07019.1.
AY091195 mRNA. Translation: AAM14134.1.
AY084716 mRNA. Translation: AAM61290.1.
PIRiC84687.
RefSeqiNP_180431.1. NM_128424.3.
UniGeneiAt.26366.

Genome annotation databases

EnsemblPlantsiAT2G28630.1; AT2G28630.1; AT2G28630.
GeneIDi817412.
GrameneiAT2G28630.1; AT2G28630.1; AT2G28630.
KEGGiath:AT2G28630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007171 Genomic DNA. Translation: AAD24372.1.
CP002685 Genomic DNA. Translation: AEC08152.1.
AY056170 mRNA. Translation: AAL07019.1.
AY091195 mRNA. Translation: AAM14134.1.
AY084716 mRNA. Translation: AAM61290.1.
PIRiC84687.
RefSeqiNP_180431.1. NM_128424.3.
UniGeneiAt.26366.

3D structure databases

ProteinModelPortaliQ9SIB2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G28630.1.

Proteomic databases

PaxDbiQ9SIB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G28630.1; AT2G28630.1; AT2G28630.
GeneIDi817412.
GrameneiAT2G28630.1; AT2G28630.1; AT2G28630.
KEGGiath:AT2G28630.

Organism-specific databases

TAIRiAT2G28630.

Phylogenomic databases

eggNOGiENOG410IH8S. Eukaryota.
ENOG410Y9JS. LUCA.
HOGENOMiHOG000238893.
InParanoidiQ9SIB2.
KOiK15397.
OMAiIAPNWYA.
OrthoDBiEOG093608SJ.
PhylomeDBiQ9SIB2.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciARA:AT2G28630-MONOMER.

Miscellaneous databases

PROiQ9SIB2.

Gene expression databases

GenevisibleiQ9SIB2. AT.

Family and domain databases

Gene3Di3.40.47.10. 3 hits.
InterProiIPR012392. 3-ktacl-CoA_syn.
IPR013747. ACP_syn_III_C.
IPR013601. FAE1_typ3_polyketide_synth.
IPR016039. Thiolase-like.
[Graphical view]
PANTHERiPTHR31561. PTHR31561. 1 hit.
PfamiPF08541. ACP_syn_III_C. 1 hit.
PF08392. FAE1_CUT1_RppA. 1 hit.
[Graphical view]
PIRSFiPIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiKCS12_ARATH
AccessioniPrimary (citable) accession number: Q9SIB2
Secondary accession number(s): Q8LFQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.