Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9SI93 (MGDG3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monogalactosyldiacylglycerol synthase 3, chloroplastic

Short name=AtMGD3
EC=2.4.1.46
Alternative name(s):
MGDG synthase type C
Gene names
Name:MGD3
Synonyms:MGDC
Ordered Locus Names:At2g11810
ORF Names:F7E22.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the synthesis of the major structural component of photosynthetic membranes and in the chloroplast envelope biogenesis. Can use both prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the eukaryotic one. Ref.1

Catalytic activity

UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

Subcellular location

Plastidchloroplast outer membrane Probable Ref.1.

Tissue specificity

Expressed mainly in roots. Detected in flowers, leaves, stems, siliques and pollen tubes. Ref.1 Ref.5

Developmental stage

Mostly expressed in early stages of development. Ref.1

Induction

Induced by phosphate deprivation. Ref.1 Ref.5 Ref.6

Miscellaneous

Auxin activates expression during Pi starvation, whereas cytokinin represses it.

Sequence similarities

Belongs to the glycosyltransferase 28 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9SI93-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9SI93-2)

The sequence of this isoform differs from the canonical sequence as follows:
     362-362: A → H
     363-465: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 465Monogalactosyldiacylglycerol synthase 3, chloroplasticPRO_0000349423

Regions

Compositional bias452 – 4554Poly-Gln

Natural variations

Alternative sequence3621A → H in isoform 2.
VSP_035389
Alternative sequence363 – 465103Missing in isoform 2.
VSP_035390

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 05E0157012E50A14

FASTA46552,990
        10         20         30         40         50         60 
MMKVVSPRTR SDSITEKVFR RVYSNFNIST VEDEYIHRQR SSDYEKESRL RKRGLEDKEE 

        70         80         90        100        110        120 
VMEMEQMGAE RIKTVLILMS DTGGGHRASA EAIRDAFKIE FGDDYRIIIK DVWKEYTGWP 

       130        140        150        160        170        180 
LNDMERQYKF MVKHVGLWSV AFHGTSPKWI HKSYLSALAA YYAKEIEAGL MEYKPDIIIS 

       190        200        210        220        230        240 
VHPLMQHIPL WVMKWQGLHK KVIFVTVITD LNTCHRTWFH HGVSRCYCPS KEVAKRALVD 

       250        260        270        280        290        300 
GLDDSQIRVF GLPVRPSFPR TILNKNELRK ELEIDLNLPA VLLMGGGEGM GPVQKTALAL 

       310        320        330        340        350        360 
GDSLYNSKES NPIGQLIVIC GRNKVLASTL ASHEWKIPVK VRGFETQMEK WMGACDCIIT 

       370        380        390        400        410        420 
KAGPGTIAEA LICGLPIILN DYIPGQEKGN VPYVVDNGAG VFTRSPKETA KIVADWFSNN 

       430        440        450        460 
KEELKKMSEN ALKLSQPEAV FDIVKDIHHL SQQQQRIPLF NEFSY 

« Hide

Isoform 2 [UniParc].

Checksum: E1898595B670EDB0
Show »

FASTA36241,602

References

« Hide 'large scale' references
[1]"Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana."
Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J.
Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation."
Kobayashi K., Awai K., Takamiya K., Ohta H.
Plant Physiol. 134:640-648(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[6]"Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokinin cross-talk."
Kobayashi K., Masuda T., Takamiya K., Ohta H.
Plant J. 47:238-248(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB047398 mRNA. Translation: BAB12041.1.
AC007187 Genomic DNA. Translation: AAD28678.2.
CP002685 Genomic DNA. Translation: AEC06183.1.
CP002685 Genomic DNA. Translation: AEC06184.1.
BT026357 mRNA. Translation: ABH04464.1.
PIRC84499.
RefSeqNP_001118301.1. NM_001124829.1. [Q9SI93-2]
NP_565352.1. NM_126865.4. [Q9SI93-1]
UniGeneAt.1646.

3D structure databases

ProteinModelPortalQ9SI93.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid1028. 2 interactions.
IntActQ9SI93. 2 interactions.

Protein family/group databases

CAZyGT28. Glycosyltransferase Family 28.

Proteomic databases

PRIDEQ9SI93.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G11810.1; AT2G11810.1; AT2G11810. [Q9SI93-1]
GeneID815657.
KEGGath:AT2G11810.

Organism-specific databases

TAIRAT2G11810.

Phylogenomic databases

eggNOGCOG0707.
HOGENOMHOG000239591.
InParanoidQ9SI93.
KOK03715.
OMACAITINH.
PhylomeDBQ9SI93.

Enzyme and pathway databases

BioCycARA:GQT-1998-MONOMER.
MetaCyc:AT2G11810-MONOMER.
BRENDA2.4.1.46. 399.

Gene expression databases

GenevestigatorQ9SI93.

Family and domain databases

InterProIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view]
PfamPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMGDG3_ARATH
AccessionPrimary (citable) accession number: Q9SI93
Secondary accession number(s): B3H4P3, Q9FZL5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names