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Q9SI93

- MGDG3_ARATH

UniProt

Q9SI93 - MGDG3_ARATH

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Protein

Monogalactosyldiacylglycerol synthase 3, chloroplastic

Gene
MGD3, MGDC, At2g11810, F7E22.4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the major structural component of photosynthetic membranes and in the chloroplast envelope biogenesis. Can use both prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the eukaryotic one.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

pH dependencei

Optimum pH is 7.0.

GO - Molecular functioni

  1. 1,2-diacylglycerol 3-beta-galactosyltransferase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. cellular response to phosphate starvation Source: TAIR
  2. fatty acid metabolic process Source: TAIR
  3. galactolipid metabolic process Source: TAIR
  4. glycolipid biosynthetic process Source: InterPro
  5. lipid glycosylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:GQT-1998-MONOMER.
MetaCyc:AT2G11810-MONOMER.
BRENDAi2.4.1.46. 399.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Monogalactosyldiacylglycerol synthase 3, chloroplastic (EC:2.4.1.46)
Short name:
AtMGD3
Alternative name(s):
MGDG synthase type C
Gene namesi
Name:MGD3
Synonyms:MGDC
Ordered Locus Names:At2g11810
ORF Names:F7E22.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G11810.

Subcellular locationi

Plastidchloroplast outer membrane Inferred 1 Publication

GO - Cellular componenti

  1. chloroplast outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 465Monogalactosyldiacylglycerol synthase 3, chloroplasticPRO_0000349423
Transit peptidei1 – ?Chloroplast Reviewed prediction

Proteomic databases

PRIDEiQ9SI93.

Expressioni

Tissue specificityi

Expressed mainly in roots. Detected in flowers, leaves, stems, siliques and pollen tubes.2 Publications

Developmental stagei

Mostly expressed in early stages of development.1 Publication

Inductioni

Induced by phosphate deprivation.3 Publications

Gene expression databases

GenevestigatoriQ9SI93.

Interactioni

Protein-protein interaction databases

BioGridi1028. 2 interactions.
IntActiQ9SI93. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9SI93.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi452 – 4554Poly-Gln

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0707.
HOGENOMiHOG000239591.
InParanoidiQ9SI93.
KOiK03715.
OMAiCAITINH.
PhylomeDBiQ9SI93.

Family and domain databases

InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SI93-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMKVVSPRTR SDSITEKVFR RVYSNFNIST VEDEYIHRQR SSDYEKESRL    50
RKRGLEDKEE VMEMEQMGAE RIKTVLILMS DTGGGHRASA EAIRDAFKIE 100
FGDDYRIIIK DVWKEYTGWP LNDMERQYKF MVKHVGLWSV AFHGTSPKWI 150
HKSYLSALAA YYAKEIEAGL MEYKPDIIIS VHPLMQHIPL WVMKWQGLHK 200
KVIFVTVITD LNTCHRTWFH HGVSRCYCPS KEVAKRALVD GLDDSQIRVF 250
GLPVRPSFPR TILNKNELRK ELEIDLNLPA VLLMGGGEGM GPVQKTALAL 300
GDSLYNSKES NPIGQLIVIC GRNKVLASTL ASHEWKIPVK VRGFETQMEK 350
WMGACDCIIT KAGPGTIAEA LICGLPIILN DYIPGQEKGN VPYVVDNGAG 400
VFTRSPKETA KIVADWFSNN KEELKKMSEN ALKLSQPEAV FDIVKDIHHL 450
SQQQQRIPLF NEFSY 465
Length:465
Mass (Da):52,990
Last modified:June 1, 2002 - v2
Checksum:i05E0157012E50A14
GO
Isoform 2 (identifier: Q9SI93-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     362-362: A → H
     363-465: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:362
Mass (Da):41,602
Checksum:iE1898595B670EDB0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei362 – 3621A → H in isoform 2. VSP_035389
Alternative sequencei363 – 465103Missing in isoform 2. VSP_035390Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB047398 mRNA. Translation: BAB12041.1.
AC007187 Genomic DNA. Translation: AAD28678.2.
CP002685 Genomic DNA. Translation: AEC06183.1.
CP002685 Genomic DNA. Translation: AEC06184.1.
BT026357 mRNA. Translation: ABH04464.1.
PIRiC84499.
RefSeqiNP_001118301.1. NM_001124829.1. [Q9SI93-2]
NP_565352.1. NM_126865.4. [Q9SI93-1]
UniGeneiAt.1646.

Genome annotation databases

EnsemblPlantsiAT2G11810.1; AT2G11810.1; AT2G11810. [Q9SI93-1]
GeneIDi815657.
KEGGiath:AT2G11810.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB047398 mRNA. Translation: BAB12041.1 .
AC007187 Genomic DNA. Translation: AAD28678.2 .
CP002685 Genomic DNA. Translation: AEC06183.1 .
CP002685 Genomic DNA. Translation: AEC06184.1 .
BT026357 mRNA. Translation: ABH04464.1 .
PIRi C84499.
RefSeqi NP_001118301.1. NM_001124829.1. [Q9SI93-2 ]
NP_565352.1. NM_126865.4. [Q9SI93-1 ]
UniGenei At.1646.

3D structure databases

ProteinModelPortali Q9SI93.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 1028. 2 interactions.
IntActi Q9SI93. 2 interactions.

Protein family/group databases

CAZyi GT28. Glycosyltransferase Family 28.

Proteomic databases

PRIDEi Q9SI93.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G11810.1 ; AT2G11810.1 ; AT2G11810 . [Q9SI93-1 ]
GeneIDi 815657.
KEGGi ath:AT2G11810.

Organism-specific databases

TAIRi AT2G11810.

Phylogenomic databases

eggNOGi COG0707.
HOGENOMi HOG000239591.
InParanoidi Q9SI93.
KOi K03715.
OMAi CAITINH.
PhylomeDBi Q9SI93.

Enzyme and pathway databases

BioCyci ARA:GQT-1998-MONOMER.
MetaCyc:AT2G11810-MONOMER.
BRENDAi 2.4.1.46. 399.

Gene expression databases

Genevestigatori Q9SI93.

Family and domain databases

InterProi IPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view ]
Pfami PF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana."
    Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J.
    Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation."
    Kobayashi K., Awai K., Takamiya K., Ohta H.
    Plant Physiol. 134:640-648(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  6. "Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokinin cross-talk."
    Kobayashi K., Masuda T., Takamiya K., Ohta H.
    Plant J. 47:238-248(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiMGDG3_ARATH
AccessioniPrimary (citable) accession number: Q9SI93
Secondary accession number(s): B3H4P3, Q9FZL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Auxin activates expression during Pi starvation, whereas cytokinin represses it.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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