Q9SI93 (MGDG3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Monogalactosyldiacylglycerol synthase 3, chloroplastic
Short name=AtMGD3
EC=2.4.1.46

Alternative name(s):
MGDG synthase type C

Gene names

Name:	MGD3
Synonyms:	MGDC
Ordered Locus Names:	At2g11810
ORF Names:	F7E22.4

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the synthesis of the major structural component of photosynthetic membranes and in the chloroplast envelope biogenesis. Can use both prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the eukaryotic one. Ref.1
Catalytic activity	UDP-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.
Subcellular location	Plastid › chloroplast outer membrane Probable Ref.1.
Tissue specificity	Expressed mainly in roots. Detected in flowers, leaves, stems, siliques and pollen tubes. Ref.1 Ref.5
Developmental stage	Mostly expressed in early stages of development. Ref.1
Induction	Induced by phosphate deprivation. Ref.1 Ref.5 Ref.6
Miscellaneous	Auxin activates expression during Pi starvation, whereas cytokinin represses it.
Sequence similarities	Belongs to the glycosyltransferase 28 family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid Plastid outer membrane
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to phosphate starvation Inferred from genetic interaction. Source: TAIR fatty acid metabolic process Inferred from mutant phenotype. Source: TAIR galactolipid metabolic process Inferred from mutant phenotype. Source: TAIR glycolipid biosynthetic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1,2-diacylglycerol 3-beta-galactosyltransferase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9SI93-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9SI93-2)

The sequence of this isoform differs from the canonical sequence as follows:
     362-362: A → H
     363-465: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 465		Monogalactosyldiacylglycerol synthase 3, chloroplastic		PRO_0000349423
Regions
	Compositional bias	452 – 455	4	Poly-Gln
Natural variations
	Alternative sequence	362	1	A → H in isoform 2.		VSP_035389
	Alternative sequence	363 – 465	103	Missing in isoform 2.		VSP_035390

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 05E0157012E50A14	FASTA	465	52,990
	10 20 30 40 50 60 MMKVVSPRTR SDSITEKVFR RVYSNFNIST VEDEYIHRQR SSDYEKESRL RKRGLEDKEE 70 80 90 100 110 120 VMEMEQMGAE RIKTVLILMS DTGGGHRASA EAIRDAFKIE FGDDYRIIIK DVWKEYTGWP 130 140 150 160 170 180 LNDMERQYKF MVKHVGLWSV AFHGTSPKWI HKSYLSALAA YYAKEIEAGL MEYKPDIIIS 190 200 210 220 230 240 VHPLMQHIPL WVMKWQGLHK KVIFVTVITD LNTCHRTWFH HGVSRCYCPS KEVAKRALVD 250 260 270 280 290 300 GLDDSQIRVF GLPVRPSFPR TILNKNELRK ELEIDLNLPA VLLMGGGEGM GPVQKTALAL 310 320 330 340 350 360 GDSLYNSKES NPIGQLIVIC GRNKVLASTL ASHEWKIPVK VRGFETQMEK WMGACDCIIT 370 380 390 400 410 420 KAGPGTIAEA LICGLPIILN DYIPGQEKGN VPYVVDNGAG VFTRSPKETA KIVADWFSNN 430 440 450 460 KEELKKMSEN ALKLSQPEAV FDIVKDIHHL SQQQQRIPLF NEFSY
	Isoform 2 [UniParc]. Checksum: E1898595B670EDB0	FASTA	362	41,602
	10 20 30 40 50 60 MMKVVSPRTR SDSITEKVFR RVYSNFNIST VEDEYIHRQR SSDYEKESRL RKRGLEDKEE 70 80 90 100 110 120 VMEMEQMGAE RIKTVLILMS DTGGGHRASA EAIRDAFKIE FGDDYRIIIK DVWKEYTGWP 130 140 150 160 170 180 LNDMERQYKF MVKHVGLWSV AFHGTSPKWI HKSYLSALAA YYAKEIEAGL MEYKPDIIIS 190 200 210 220 230 240 VHPLMQHIPL WVMKWQGLHK KVIFVTVITD LNTCHRTWFH HGVSRCYCPS KEVAKRALVD 250 260 270 280 290 300 GLDDSQIRVF GLPVRPSFPR TILNKNELRK ELEIDLNLPA VLLMGGGEGM GPVQKTALAL 310 320 330 340 350 360 GDSLYNSKES NPIGQLIVIC GRNKVLASTL ASHEWKIPVK VRGFETQMEK WMGACDCIIT KH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana." Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J. Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed: 11553816] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation." Kobayashi K., Awai K., Takamiya K., Ohta H. Plant Physiol. 134:640-648(2004) [PubMed: 14730084] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[6]	"Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokinin cross-talk." Kobayashi K., Masuda T., Takamiya K., Ohta H. Plant J. 47:238-248(2006) [PubMed: 16762032] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB047398 mRNA. Translation: BAB12041.1. AC007187 Genomic DNA. Translation: AAD28678.2. CP002685 Genomic DNA. Translation: AEC06183.1. CP002685 Genomic DNA. Translation: AEC06184.1. BT026357 mRNA. Translation: ABH04464.1.
IPI	IPI00533108. IPI00891201.
PIR	C84499.
RefSeq	NP_001118301.1. NM_001124829.1. NP_565352.1. NM_126865.4.
UniGene	At.1646.
3D structure databases
ProteinModelPortal	Q9SI93.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SI93. 2 interactions.
STRING	Q9SI93.
Protein family/group databases
CAZy	GT28. Glycosyltransferase Family 28.
Proteomic databases
PRIDE	Q9SI93.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G11810.1; AT2G11810.1; AT2G11810.
GeneID	815657.
GenomeReviews	Gene locus AT2G11810 in contig CT485783_GR.
KEGG	ath:AT2G11810.
NMPDR	fig|3702.1.peg.8353.
Organism-specific databases
TAIR	At2g11810.
Phylogenomic databases
eggNOG	COG0707.
GeneTree	EPGT00050000017026.
HOGENOM	HBG601852.
InParanoid	Q9SI93.
OMA	QLIVICG.
PhylomeDB	Q9SI93.
ProtClustDB	PLN02605.
Enzyme and pathway databases
BRENDA	2.4.1.46. 399.
Gene expression databases
ArrayExpress	Q9SI93.
Genevestigator	Q9SI93.
Family and domain databases
InterPro	IPR009695. Diacylglyc_glucosyltr_N. IPR007235. Glyco_trans_28_C. [Graphical view]
KO	K03715.
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF06925. MGDG_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGDG3_ARATH

Accession

Primary (citable) accession number: Q9SI93
Secondary accession number(s): B3H4P3, Q9FZL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program