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Q9SI93

- MGDG3_ARATH

UniProt

Q9SI93 - MGDG3_ARATH

Protein

Monogalactosyldiacylglycerol synthase 3, chloroplastic

Gene

MGD3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of the major structural component of photosynthetic membranes and in the chloroplast envelope biogenesis. Can use both prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the eukaryotic one.1 Publication

    Catalytic activityi

    UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

    pH dependencei

    Optimum pH is 7.0.

    GO - Molecular functioni

    1. 1,2-diacylglycerol 3-beta-galactosyltransferase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. cellular response to phosphate starvation Source: TAIR
    2. fatty acid metabolic process Source: TAIR
    3. galactolipid metabolic process Source: TAIR
    4. glycolipid biosynthetic process Source: InterPro
    5. lipid glycosylation Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciARA:GQT-1998-MONOMER.
    MetaCyc:AT2G11810-MONOMER.
    BRENDAi2.4.1.46. 399.

    Protein family/group databases

    CAZyiGT28. Glycosyltransferase Family 28.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monogalactosyldiacylglycerol synthase 3, chloroplastic (EC:2.4.1.46)
    Short name:
    AtMGD3
    Alternative name(s):
    MGDG synthase type C
    Gene namesi
    Name:MGD3
    Synonyms:MGDC
    Ordered Locus Names:At2g11810
    ORF Names:F7E22.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G11810.

    Subcellular locationi

    Plastidchloroplast outer membrane 1 Publication

    GO - Cellular componenti

    1. chloroplast outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Plastid outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 465Monogalactosyldiacylglycerol synthase 3, chloroplasticPRO_0000349423
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Proteomic databases

    PRIDEiQ9SI93.

    Expressioni

    Tissue specificityi

    Expressed mainly in roots. Detected in flowers, leaves, stems, siliques and pollen tubes.2 Publications

    Developmental stagei

    Mostly expressed in early stages of development.1 Publication

    Inductioni

    Induced by phosphate deprivation.3 Publications

    Gene expression databases

    GenevestigatoriQ9SI93.

    Interactioni

    Protein-protein interaction databases

    BioGridi1028. 7 interactions.
    IntActiQ9SI93. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SI93.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi452 – 4554Poly-Gln

    Sequence similaritiesi

    Belongs to the glycosyltransferase 28 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0707.
    HOGENOMiHOG000239591.
    InParanoidiQ9SI93.
    KOiK03715.
    OMAiCAITINH.
    PhylomeDBiQ9SI93.

    Family and domain databases

    InterProiIPR009695. Diacylglyc_glucosyltr_N.
    IPR007235. Glyco_trans_28_C.
    [Graphical view]
    PfamiPF04101. Glyco_tran_28_C. 1 hit.
    PF06925. MGDG_synth. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9SI93-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMKVVSPRTR SDSITEKVFR RVYSNFNIST VEDEYIHRQR SSDYEKESRL    50
    RKRGLEDKEE VMEMEQMGAE RIKTVLILMS DTGGGHRASA EAIRDAFKIE 100
    FGDDYRIIIK DVWKEYTGWP LNDMERQYKF MVKHVGLWSV AFHGTSPKWI 150
    HKSYLSALAA YYAKEIEAGL MEYKPDIIIS VHPLMQHIPL WVMKWQGLHK 200
    KVIFVTVITD LNTCHRTWFH HGVSRCYCPS KEVAKRALVD GLDDSQIRVF 250
    GLPVRPSFPR TILNKNELRK ELEIDLNLPA VLLMGGGEGM GPVQKTALAL 300
    GDSLYNSKES NPIGQLIVIC GRNKVLASTL ASHEWKIPVK VRGFETQMEK 350
    WMGACDCIIT KAGPGTIAEA LICGLPIILN DYIPGQEKGN VPYVVDNGAG 400
    VFTRSPKETA KIVADWFSNN KEELKKMSEN ALKLSQPEAV FDIVKDIHHL 450
    SQQQQRIPLF NEFSY 465
    Length:465
    Mass (Da):52,990
    Last modified:June 1, 2002 - v2
    Checksum:i05E0157012E50A14
    GO
    Isoform 2 (identifier: Q9SI93-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         362-362: A → H
         363-465: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:362
    Mass (Da):41,602
    Checksum:iE1898595B670EDB0
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei362 – 3621A → H in isoform 2. CuratedVSP_035389
    Alternative sequencei363 – 465103Missing in isoform 2. CuratedVSP_035390Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB047398 mRNA. Translation: BAB12041.1.
    AC007187 Genomic DNA. Translation: AAD28678.2.
    CP002685 Genomic DNA. Translation: AEC06183.1.
    CP002685 Genomic DNA. Translation: AEC06184.1.
    BT026357 mRNA. Translation: ABH04464.1.
    PIRiC84499.
    RefSeqiNP_001118301.1. NM_001124829.1. [Q9SI93-2]
    NP_565352.1. NM_126865.4. [Q9SI93-1]
    UniGeneiAt.1646.

    Genome annotation databases

    EnsemblPlantsiAT2G11810.1; AT2G11810.1; AT2G11810. [Q9SI93-1]
    GeneIDi815657.
    KEGGiath:AT2G11810.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB047398 mRNA. Translation: BAB12041.1 .
    AC007187 Genomic DNA. Translation: AAD28678.2 .
    CP002685 Genomic DNA. Translation: AEC06183.1 .
    CP002685 Genomic DNA. Translation: AEC06184.1 .
    BT026357 mRNA. Translation: ABH04464.1 .
    PIRi C84499.
    RefSeqi NP_001118301.1. NM_001124829.1. [Q9SI93-2 ]
    NP_565352.1. NM_126865.4. [Q9SI93-1 ]
    UniGenei At.1646.

    3D structure databases

    ProteinModelPortali Q9SI93.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 1028. 7 interactions.
    IntActi Q9SI93. 2 interactions.

    Protein family/group databases

    CAZyi GT28. Glycosyltransferase Family 28.

    Proteomic databases

    PRIDEi Q9SI93.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G11810.1 ; AT2G11810.1 ; AT2G11810 . [Q9SI93-1 ]
    GeneIDi 815657.
    KEGGi ath:AT2G11810.

    Organism-specific databases

    TAIRi AT2G11810.

    Phylogenomic databases

    eggNOGi COG0707.
    HOGENOMi HOG000239591.
    InParanoidi Q9SI93.
    KOi K03715.
    OMAi CAITINH.
    PhylomeDBi Q9SI93.

    Enzyme and pathway databases

    BioCyci ARA:GQT-1998-MONOMER.
    MetaCyc:AT2G11810-MONOMER.
    BRENDAi 2.4.1.46. 399.

    Gene expression databases

    Genevestigatori Q9SI93.

    Family and domain databases

    InterProi IPR009695. Diacylglyc_glucosyltr_N.
    IPR007235. Glyco_trans_28_C.
    [Graphical view ]
    Pfami PF04101. Glyco_tran_28_C. 1 hit.
    PF06925. MGDG_synth. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana."
      Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J.
      Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION.
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation."
      Kobayashi K., Awai K., Takamiya K., Ohta H.
      Plant Physiol. 134:640-648(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    6. "Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokinin cross-talk."
      Kobayashi K., Masuda T., Takamiya K., Ohta H.
      Plant J. 47:238-248(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiMGDG3_ARATH
    AccessioniPrimary (citable) accession number: Q9SI93
    Secondary accession number(s): B3H4P3, Q9FZL5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Auxin activates expression during Pi starvation, whereas cytokinin represses it.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3