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Q9SI62

- POED2_ARATH

UniProt

Q9SI62 - POED2_ARATH

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Protein
Polyprenol reductase 2
Gene
At2g16530, F1P15.9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism By similarity.

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

Pathwayi

GO - Molecular functioni

  1. oxidoreductase activity, acting on the CH-CH group of donors Source: InterPro

GO - Biological processi

  1. lipid metabolic process Source: InterPro
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_185286. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase 2 (EC:1.3.1.94)
Gene namesi
Ordered Locus Names:At2g16530
ORF Names:F1P15.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G16530.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221Helical; Reviewed prediction
Add
BLAST
Transmembranei66 – 8621Helical; Reviewed prediction
Add
BLAST
Transmembranei164 – 18421Helical; Reviewed prediction
Add
BLAST
Transmembranei223 – 24321Helical; Reviewed prediction
Add
BLAST
Transmembranei266 – 28621Helical; Reviewed prediction
Add
BLAST
Transmembranei291 – 31121Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. endoplasmic reticulum Source: TAIR
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Polyprenol reductase 2
PRO_0000398656Add
BLAST

Proteomic databases

PaxDbiQ9SI62.
PRIDEiQ9SI62.

Expressioni

Gene expression databases

GenevestigatoriQ9SI62.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G16530.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SI62.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG330066.
HOGENOMiHOG000240963.
InParanoidiQ9SI62.
OMAiTSAPEFN.
PhylomeDBiQ9SI62.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SI62-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVELEIVWLV RGAWITVWIV SILPLVIASI PTSKLNSFRE LVLSFAGRGK    50
ILHPSSQKFT IPQKCFAHFY VIGVVWTTLL LAATWMYACK MAPLSSEEFQ 100
LSDIASRLAG GSDVFSVHKS NMTPVEHRFK VWRAVFLLLL MEIHVLRRLI 150
ESFYVFKYSP SARMHILGYF AGLFFYVTAP LSLCSNIAPE VAGFVGNQVA 200
EFIANGKSHT SAPEFNLLSS ISPLMKLGSL QWIGGAIFLW GWIHQRRCHA 250
ILGSLRENPS QAKEYIIPYG DWFGMVSSPH FLAEIVLYAG LLIASGGTDI 300
TIWLLFGFVA ANLTYAAGET HRWYLRKFEN YPANRHAIFP YVY 343
Length:343
Mass (Da):38,678
Last modified:June 1, 2002 - v2
Checksum:i49AB19FF40A9FA06
GO
Isoform 2 (identifier: Q9SI62-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     58-58: Missing.

Note: No experimental confirmation available.

Show »
Length:342
Mass (Da):38,550
Checksum:i3510ED8C0A5AA451
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei58 – 581Missing in isoform 2.
VSP_039793

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661F → L in BX819600. 1 Publication
Sequence conflicti69 – 691F → L in AAK96489. 1 Publication
Sequence conflicti69 – 691F → L in AAO11632. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007195 Genomic DNA. Translation: AAD26491.2.
CP002685 Genomic DNA. Translation: AEC06507.1.
CP002685 Genomic DNA. Translation: AEC06508.1.
AY052296 mRNA. Translation: AAK96489.1.
BT002716 mRNA. Translation: AAO11632.1.
BX819600 mRNA. No translation available.
PIRiD84541.
RefSeqiNP_565389.1. NM_127207.3. [Q9SI62-1]
NP_973474.1. NM_201745.1. [Q9SI62-2]
UniGeneiAt.14490.
At.48730.

Genome annotation databases

EnsemblPlantsiAT2G16530.1; AT2G16530.1; AT2G16530. [Q9SI62-1]
GeneIDi816152.
KEGGiath:AT2G16530.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007195 Genomic DNA. Translation: AAD26491.2 .
CP002685 Genomic DNA. Translation: AEC06507.1 .
CP002685 Genomic DNA. Translation: AEC06508.1 .
AY052296 mRNA. Translation: AAK96489.1 .
BT002716 mRNA. Translation: AAO11632.1 .
BX819600 mRNA. No translation available.
PIRi D84541.
RefSeqi NP_565389.1. NM_127207.3. [Q9SI62-1 ]
NP_973474.1. NM_201745.1. [Q9SI62-2 ]
UniGenei At.14490.
At.48730.

3D structure databases

ProteinModelPortali Q9SI62.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G16530.1-P.

Proteomic databases

PaxDbi Q9SI62.
PRIDEi Q9SI62.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G16530.1 ; AT2G16530.1 ; AT2G16530 . [Q9SI62-1 ]
GeneIDi 816152.
KEGGi ath:AT2G16530.

Organism-specific databases

TAIRi AT2G16530.

Phylogenomic databases

eggNOGi NOG330066.
HOGENOMi HOG000240963.
InParanoidi Q9SI62.
OMAi TSAPEFN.
PhylomeDBi Q9SI62.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_185286. Synthesis of Dolichyl-phosphate.

Gene expression databases

Genevestigatori Q9SI62.

Family and domain databases

InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view ]
Pfami PF02544. Steroid_dh. 1 hit.
[Graphical view ]
PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPOED2_ARATH
AccessioniPrimary (citable) accession number: Q9SI62
Secondary accession number(s): Q3EBZ6, Q941B4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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