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Protein

Polyprenol reductase 2

Gene

PPRD2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Involved in the regulation of plant growth and reproductive processes.1 Publication

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • lipid metabolic process Source: InterPro
  • protein N-linked glycosylation Source: TAIR

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

ReactomeiR-ATH-193048. Androgen biosynthesis.
R-ATH-446199. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase 21 Publication (EC:1.3.1.941 Publication)
Gene namesi
Name:PPRD21 Publication
Ordered Locus Names:At2g16530Imported
ORF Names:F1P15.9Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G16530.
TAIRilocus:2045126. AT2G16530.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Transmembranei164 – 184HelicalSequence analysisAdd BLAST21
Transmembranei223 – 243HelicalSequence analysisAdd BLAST21
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Transmembranei291 – 311HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Male sterility, when homozygous. Deformed and non-viable pollen grains.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003986561 – 343Polyprenol reductase 2Add BLAST343

Proteomic databases

PaxDbiQ9SI62.

PTM databases

iPTMnetiQ9SI62.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and flowers.1 Publication

Gene expression databases

GenevisibleiQ9SI62. AT.

Interactioni

Protein-protein interaction databases

BioGridi1510. 1 interactor.
STRINGi3702.AT2G16530.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SI62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1640. Eukaryota.
ENOG4111JQM. LUCA.
HOGENOMiHOG000240963.
InParanoidiQ9SI62.
KOiK12345.
OMAiTSAPEFN.
OrthoDBiEOG09360J9J.
PhylomeDBiQ9SI62.

Family and domain databases

InterProiView protein in InterPro
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
PfamiView protein in Pfam
PF02544. Steroid_dh. 1 hit.
PROSITEiView protein in PROSITE
PS50244. S5A_REDUCTASE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SI62-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVELEIVWLV RGAWITVWIV SILPLVIASI PTSKLNSFRE LVLSFAGRGK
60 70 80 90 100
ILHPSSQKFT IPQKCFAHFY VIGVVWTTLL LAATWMYACK MAPLSSEEFQ
110 120 130 140 150
LSDIASRLAG GSDVFSVHKS NMTPVEHRFK VWRAVFLLLL MEIHVLRRLI
160 170 180 190 200
ESFYVFKYSP SARMHILGYF AGLFFYVTAP LSLCSNIAPE VAGFVGNQVA
210 220 230 240 250
EFIANGKSHT SAPEFNLLSS ISPLMKLGSL QWIGGAIFLW GWIHQRRCHA
260 270 280 290 300
ILGSLRENPS QAKEYIIPYG DWFGMVSSPH FLAEIVLYAG LLIASGGTDI
310 320 330 340
TIWLLFGFVA ANLTYAAGET HRWYLRKFEN YPANRHAIFP YVY
Length:343
Mass (Da):38,678
Last modified:June 1, 2002 - v2
Checksum:i49AB19FF40A9FA06
GO
Isoform 2 (identifier: Q9SI62-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     58-58: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:342
Mass (Da):38,550
Checksum:i3510ED8C0A5AA451
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66F → L in BX819600 (PubMed:14993207).Curated1
Sequence conflicti69F → L in AAK96489 (PubMed:14593172).Curated1
Sequence conflicti69F → L in AAO11632 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03979358Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007195 Genomic DNA. Translation: AAD26491.2.
CP002685 Genomic DNA. Translation: AEC06507.1.
CP002685 Genomic DNA. Translation: AEC06508.1.
CP002685 Genomic DNA. Translation: ANM63071.1.
AY052296 mRNA. Translation: AAK96489.1.
BT002716 mRNA. Translation: AAO11632.1.
BX819600 mRNA. No translation available.
PIRiD84541.
RefSeqiNP_001325183.1. NM_001335479.1. [Q9SI62-1]
NP_565389.1. NM_127207.4. [Q9SI62-1]
NP_973474.1. NM_201745.2. [Q9SI62-2]
UniGeneiAt.14490.
At.48730.

Genome annotation databases

EnsemblPlantsiAT2G16530.1; AT2G16530.1; AT2G16530. [Q9SI62-1]
AT2G16530.4; AT2G16530.4; AT2G16530. [Q9SI62-1]
GeneIDi816152.
GrameneiAT2G16530.1; AT2G16530.1; AT2G16530. [Q9SI62-1]
AT2G16530.4; AT2G16530.4; AT2G16530. [Q9SI62-1]
KEGGiath:AT2G16530.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPOED2_ARATH
AccessioniPrimary (citable) accession number: Q9SI62
Secondary accession number(s): Q3EBZ6, Q941B4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2002
Last modified: July 5, 2017
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families