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Q9SI62

- POED2_ARATH

UniProt

Q9SI62 - POED2_ARATH

Protein

Polyprenol reductase 2

Gene

At2g16530

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism By similarity.By similarity

    Catalytic activityi

    Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

    Pathwayi

    GO - Molecular functioni

    1. oxidoreductase activity, acting on the CH-CH group of donors Source: InterPro

    GO - Biological processi

    1. lipid metabolic process Source: InterPro
    2. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    ReactomeiREACT_185286. Synthesis of Dolichyl-phosphate.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyprenol reductase 2 (EC:1.3.1.94)
    Gene namesi
    Ordered Locus Names:At2g16530
    ORF Names:F1P15.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G16530.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. Golgi apparatus Source: TAIR
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 343343Polyprenol reductase 2PRO_0000398656Add
    BLAST

    Proteomic databases

    PaxDbiQ9SI62.
    PRIDEiQ9SI62.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9SI62.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G16530.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SI62.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei66 – 8621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei164 – 18421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei223 – 24321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei266 – 28621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei291 – 31121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG330066.
    HOGENOMiHOG000240963.
    InParanoidiQ9SI62.
    OMAiTSAPEFN.
    PhylomeDBiQ9SI62.

    Family and domain databases

    InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9SI62-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVELEIVWLV RGAWITVWIV SILPLVIASI PTSKLNSFRE LVLSFAGRGK    50
    ILHPSSQKFT IPQKCFAHFY VIGVVWTTLL LAATWMYACK MAPLSSEEFQ 100
    LSDIASRLAG GSDVFSVHKS NMTPVEHRFK VWRAVFLLLL MEIHVLRRLI 150
    ESFYVFKYSP SARMHILGYF AGLFFYVTAP LSLCSNIAPE VAGFVGNQVA 200
    EFIANGKSHT SAPEFNLLSS ISPLMKLGSL QWIGGAIFLW GWIHQRRCHA 250
    ILGSLRENPS QAKEYIIPYG DWFGMVSSPH FLAEIVLYAG LLIASGGTDI 300
    TIWLLFGFVA ANLTYAAGET HRWYLRKFEN YPANRHAIFP YVY 343
    Length:343
    Mass (Da):38,678
    Last modified:June 1, 2002 - v2
    Checksum:i49AB19FF40A9FA06
    GO
    Isoform 2 (identifier: Q9SI62-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         58-58: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:342
    Mass (Da):38,550
    Checksum:i3510ED8C0A5AA451
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti66 – 661F → L in BX819600. (PubMed:14993207)Curated
    Sequence conflicti69 – 691F → L in AAK96489. (PubMed:14593172)Curated
    Sequence conflicti69 – 691F → L in AAO11632. (PubMed:14593172)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei58 – 581Missing in isoform 2. 1 PublicationVSP_039793

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007195 Genomic DNA. Translation: AAD26491.2.
    CP002685 Genomic DNA. Translation: AEC06507.1.
    CP002685 Genomic DNA. Translation: AEC06508.1.
    AY052296 mRNA. Translation: AAK96489.1.
    BT002716 mRNA. Translation: AAO11632.1.
    BX819600 mRNA. No translation available.
    PIRiD84541.
    RefSeqiNP_565389.1. NM_127207.3. [Q9SI62-1]
    NP_973474.1. NM_201745.1. [Q9SI62-2]
    UniGeneiAt.14490.
    At.48730.

    Genome annotation databases

    EnsemblPlantsiAT2G16530.1; AT2G16530.1; AT2G16530. [Q9SI62-1]
    GeneIDi816152.
    KEGGiath:AT2G16530.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007195 Genomic DNA. Translation: AAD26491.2 .
    CP002685 Genomic DNA. Translation: AEC06507.1 .
    CP002685 Genomic DNA. Translation: AEC06508.1 .
    AY052296 mRNA. Translation: AAK96489.1 .
    BT002716 mRNA. Translation: AAO11632.1 .
    BX819600 mRNA. No translation available.
    PIRi D84541.
    RefSeqi NP_565389.1. NM_127207.3. [Q9SI62-1 ]
    NP_973474.1. NM_201745.1. [Q9SI62-2 ]
    UniGenei At.14490.
    At.48730.

    3D structure databases

    ProteinModelPortali Q9SI62.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G16530.1-P.

    Proteomic databases

    PaxDbi Q9SI62.
    PRIDEi Q9SI62.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G16530.1 ; AT2G16530.1 ; AT2G16530 . [Q9SI62-1 ]
    GeneIDi 816152.
    KEGGi ath:AT2G16530.

    Organism-specific databases

    TAIRi AT2G16530.

    Phylogenomic databases

    eggNOGi NOG330066.
    HOGENOMi HOG000240963.
    InParanoidi Q9SI62.
    OMAi TSAPEFN.
    PhylomeDBi Q9SI62.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_185286. Synthesis of Dolichyl-phosphate.

    Gene expression databases

    Genevestigatori Q9SI62.

    Family and domain databases

    InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiPOED2_ARATH
    AccessioniPrimary (citable) accession number: Q9SI62
    Secondary accession number(s): Q3EBZ6, Q941B4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3