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Q9SI61

- ASE1_ARATH

UniProt

Q9SI61 - ASE1_ARATH

Protein

Amidophosphoribosyltransferase 1, chloroplastic

Gene

ASE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the first committed step of 'de novo' purine biosynthesis from glutamine. Involved in plastid biogenesis and cell division.1 Publication

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.By similarity
    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei91 – 911NucleophilePROSITE-ProRule annotation
    Metal bindingi327 – 3271Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi473 – 4731Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi524 – 5241Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi527 – 5271Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. amidophosphoribosyltransferase activity Source: TAIR
    2. iron-sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G16570-MONOMER.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.A01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase 1, chloroplastic (EC:2.4.2.14)
    Short name:
    AtATase1
    Short name:
    PRPP1
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase 1
    Short name:
    AtGPRAT1
    Gene namesi
    Name:ASE1
    Synonyms:GPRAT1
    Ordered Locus Names:At2g16570
    ORF Names:F1P15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G16570.

    Subcellular locationi

    Plastidchloroplast stroma 1 Publication

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. cytosol Source: TAIR
    4. plastid Source: TAIR
    5. plastid stroma Source: UniProtKB

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5858ChloroplastSequence AnalysisAdd
    BLAST
    Chaini59 – 566508Amidophosphoribosyltransferase 1, chloroplasticPRO_0000420281Add
    BLAST

    Proteomic databases

    PRIDEiQ9SI61.

    Expressioni

    Tissue specificityi

    Expressed in flowers and roots. Also present in leaves, and, to a lower extent, in cotyledons.2 Publications

    Gene expression databases

    GenevestigatoriQ9SI61.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9SI61. 1 interaction.
    STRINGi3702.AT2G16570.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SI61.
    SMRiQ9SI61. Positions 91-535.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini91 – 311221Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Transit peptide

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033688.
    InParanoidiQ9SI61.
    KOiK00764.
    OMAiRIIDACE.
    PhylomeDBiQ9SI61.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SI61-1 [UniParc]FASTAAdd to Basket

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    MAATTSFSSS LSLITKPNNS SYTNQPLPLF PKPFLKPPHL SLLPSPLSSP    50
    PPSLIHGVSS YFSSPSPSED NSHTPFDYHN DEDDEKPREE CGVVGIYGDP 100
    EASRLCYLAL HALQHRGQEG AGIVTVSPEK VLQTITGVGL VSEVFNESKL 150
    DQLPGEFAIG HVRYSTAGAS MLKNVQPFVA GYRFGSIGVA HNGNLVNYKT 200
    LRAMLEENGS IFNTSSDTEV VLHLIAISKA RPFFMRIIDA CEKLQGAYSM 250
    VFVTEDKLVA VRDPYGFRPL VMGRRSNGAV VFASETCALD LIEATYEREV 300
    YPGEVLVVDK DGVKSQCLMP KFEPKQCIFE HIYFSLPNSI VFGRSVYESR 350
    HVFGEILATE SPVECDVVIA VPDSGVVAAL GYAAKSGVPF QQGLIRSHYV 400
    GRTFIEPSQK IRDFGVKLKL SPVRGVLEGK RVVVVDDSIV RGTTSSKIVR 450
    LLREAGAKEV HMRIASPPIV ASCYYGVDTP SSEELISNRL SVEEINEFIG 500
    SDSLAFLSFD TLKKHLGKDS KSFCYACFTG DYPVKPTEVK VKRGGGDFID 550
    DGLVGSFENI EAGWVR 566
    Length:566
    Mass (Da):61,842
    Last modified:May 1, 2000 - v1
    Checksum:i887827A9FEB901E0
    GO

    Sequence cautioni

    The sequence BAA06023.1 differs from that shown. Reason: Frameshift at position 70.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti74 – 741T → A in BAA06023. (PubMed:7948903)Curated
    Sequence conflicti106 – 1061C → F in BAA06023. (PubMed:7948903)Curated
    Sequence conflicti160 – 1601G → A in BAA06023. (PubMed:7948903)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D28868 mRNA. Translation: BAA06023.1. Frameshift.
    AC007195 Genomic DNA. Translation: AAD26498.1.
    CP002685 Genomic DNA. Translation: AEC06510.1.
    PIRiE84541.
    S52622.
    RefSeqiNP_179247.1. NM_127208.1.
    UniGeneiAt.14491.

    Genome annotation databases

    EnsemblPlantsiAT2G16570.1; AT2G16570.1; AT2G16570.
    GeneIDi816156.
    KEGGiath:AT2G16570.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D28868 mRNA. Translation: BAA06023.1 . Frameshift.
    AC007195 Genomic DNA. Translation: AAD26498.1 .
    CP002685 Genomic DNA. Translation: AEC06510.1 .
    PIRi E84541.
    S52622.
    RefSeqi NP_179247.1. NM_127208.1.
    UniGenei At.14491.

    3D structure databases

    ProteinModelPortali Q9SI61.
    SMRi Q9SI61. Positions 91-535.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9SI61. 1 interaction.
    STRINGi 3702.AT2G16570.1-P.

    Protein family/group databases

    MEROPSi C44.A01.

    Proteomic databases

    PRIDEi Q9SI61.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G16570.1 ; AT2G16570.1 ; AT2G16570 .
    GeneIDi 816156.
    KEGGi ath:AT2G16570.

    Organism-specific databases

    GeneFarmi 2372. 186.
    TAIRi AT2G16570.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033688.
    InParanoidi Q9SI61.
    KOi K00764.
    OMAi RIIDACE.
    PhylomeDBi Q9SI61.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    BioCyci ARA:AT2G16570-MONOMER.

    Gene expression databases

    Genevestigatori Q9SI61.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two amidophosphoribosyltransferase genes of Arabidopsis thaliana expressed in different organs."
      Ito T., Shiraishi H., Okada K., Shimura Y.
      Plant Mol. Biol. 26:529-533(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Landsberg erecta.
      Tissue: Flower and Root.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Characterization of Arabidopsis glutamine phosphoribosyl pyrophosphate amidotransferase-deficient mutants."
      Hung W.-F., Chen L.-J., Boldt R., Sun C.-W., Li H.-M.
      Plant Physiol. 135:1314-1323(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiASE1_ARATH
    AccessioniPrimary (citable) accession number: Q9SI61
    Secondary accession number(s): Q38999
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3