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Q9SI61 (ASE1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase 1, chloroplastic

Short name=AtATase1
Short name=PRPP1
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase 1
Short name=AtGPRAT1
Gene names
Name:ASE1
Synonyms:GPRAT1
Ordered Locus Names:At2g16570
ORF Names:F1P15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the first committed step of 'de novo' purine biosynthesis from glutamine. Involved in plastid biogenesis and cell division. Ref.4

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity.

Binds 1 magnesium ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Subcellular location

Plastidchloroplast stroma Ref.4.

Tissue specificity

Expressed in flowers and roots. Also present in leaves, and, to a lower extent, in cotyledons. Ref.1 Ref.4

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence caution

The sequence BAA06023.1 differs from that shown. Reason: Frameshift at position 70.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5858Chloroplast Potential
Chain59 – 566508Amidophosphoribosyltransferase 1, chloroplastic
PRO_0000420281

Regions

Domain91 – 311221Glutamine amidotransferase type-2

Sites

Active site911Nucleophile By similarity
Metal binding3271Iron-sulfur (4Fe-4S) By similarity
Metal binding4731Iron-sulfur (4Fe-4S) By similarity
Metal binding5241Iron-sulfur (4Fe-4S) By similarity
Metal binding5271Iron-sulfur (4Fe-4S) By similarity

Experimental info

Sequence conflict741T → A in BAA06023. Ref.1
Sequence conflict1061C → F in BAA06023. Ref.1
Sequence conflict1601G → A in BAA06023. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9SI61 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 887827A9FEB901E0

FASTA56661,842
        10         20         30         40         50         60 
MAATTSFSSS LSLITKPNNS SYTNQPLPLF PKPFLKPPHL SLLPSPLSSP PPSLIHGVSS 

        70         80         90        100        110        120 
YFSSPSPSED NSHTPFDYHN DEDDEKPREE CGVVGIYGDP EASRLCYLAL HALQHRGQEG 

       130        140        150        160        170        180 
AGIVTVSPEK VLQTITGVGL VSEVFNESKL DQLPGEFAIG HVRYSTAGAS MLKNVQPFVA 

       190        200        210        220        230        240 
GYRFGSIGVA HNGNLVNYKT LRAMLEENGS IFNTSSDTEV VLHLIAISKA RPFFMRIIDA 

       250        260        270        280        290        300 
CEKLQGAYSM VFVTEDKLVA VRDPYGFRPL VMGRRSNGAV VFASETCALD LIEATYEREV 

       310        320        330        340        350        360 
YPGEVLVVDK DGVKSQCLMP KFEPKQCIFE HIYFSLPNSI VFGRSVYESR HVFGEILATE 

       370        380        390        400        410        420 
SPVECDVVIA VPDSGVVAAL GYAAKSGVPF QQGLIRSHYV GRTFIEPSQK IRDFGVKLKL 

       430        440        450        460        470        480 
SPVRGVLEGK RVVVVDDSIV RGTTSSKIVR LLREAGAKEV HMRIASPPIV ASCYYGVDTP 

       490        500        510        520        530        540 
SSEELISNRL SVEEINEFIG SDSLAFLSFD TLKKHLGKDS KSFCYACFTG DYPVKPTEVK 

       550        560 
VKRGGGDFID DGLVGSFENI EAGWVR 

« Hide

References

« Hide 'large scale' references
[1]"Two amidophosphoribosyltransferase genes of Arabidopsis thaliana expressed in different organs."
Ito T., Shiraishi H., Okada K., Shimura Y.
Plant Mol. Biol. 26:529-533(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: cv. Landsberg erecta.
Tissue: Flower and Root.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Characterization of Arabidopsis glutamine phosphoribosyl pyrophosphate amidotransferase-deficient mutants."
Hung W.-F., Chen L.-J., Boldt R., Sun C.-W., Li H.-M.
Plant Physiol. 135:1314-1323(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D28868 mRNA. Translation: BAA06023.1. Frameshift.
AC007195 Genomic DNA. Translation: AAD26498.1.
CP002685 Genomic DNA. Translation: AEC06510.1.
PIRE84541.
S52622.
RefSeqNP_179247.1. NM_127208.1.
UniGeneAt.14491.

3D structure databases

ProteinModelPortalQ9SI61.
SMRQ9SI61. Positions 91-535.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9SI61. 1 interaction.
STRING3702.AT2G16570.1-P.

Protein family/group databases

MEROPSC44.A01.

Proteomic databases

PRIDEQ9SI61.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G16570.1; AT2G16570.1; AT2G16570.
GeneID816156.
KEGGath:AT2G16570.

Organism-specific databases

GeneFarm2372. 186.
TAIRAT2G16570.

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033688.
InParanoidQ9SI61.
KOK00764.
OMARIIDACE.
PhylomeDBQ9SI61.

Enzyme and pathway databases

BioCycARA:AT2G16570-MONOMER.
UniPathwayUPA00074; UER00124.

Gene expression databases

GenevestigatorQ9SI61.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASE1_ARATH
AccessionPrimary (citable) accession number: Q9SI61
Secondary accession number(s): Q38999
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names