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Q9SI61

- ASE1_ARATH

UniProt

Q9SI61 - ASE1_ARATH

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Protein

Amidophosphoribosyltransferase 1, chloroplastic

Gene

ASE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the first committed step of 'de novo' purine biosynthesis from glutamine. Involved in plastid biogenesis and cell division.1 Publication

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 4Fe-4S cluster per subunit.By similarity
Binds 1 magnesium ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911NucleophilePROSITE-ProRule annotation
Metal bindingi327 – 3271Iron-sulfur (4Fe-4S)By similarity
Metal bindingi473 – 4731Iron-sulfur (4Fe-4S)By similarity
Metal bindingi524 – 5241Iron-sulfur (4Fe-4S)By similarity
Metal bindingi527 – 5271Iron-sulfur (4Fe-4S)By similarity

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: TAIR
  2. iron-sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G16570-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase 1, chloroplastic (EC:2.4.2.14)
Short name:
AtATase1
Short name:
PRPP1
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase 1
Short name:
AtGPRAT1
Gene namesi
Name:ASE1
Synonyms:GPRAT1
Ordered Locus Names:At2g16570
ORF Names:F1P15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G16570.

Subcellular locationi

Plastidchloroplast stroma 1 Publication

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. cytosol Source: TAIR
  4. plastid Source: TAIR
  5. plastid stroma Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5858ChloroplastSequence AnalysisAdd
BLAST
Chaini59 – 566508Amidophosphoribosyltransferase 1, chloroplasticPRO_0000420281Add
BLAST

Proteomic databases

PRIDEiQ9SI61.

Expressioni

Tissue specificityi

Expressed in flowers and roots. Also present in leaves, and, to a lower extent, in cotyledons.2 Publications

Gene expression databases

GenevestigatoriQ9SI61.

Interactioni

Protein-protein interaction databases

IntActiQ9SI61. 1 interaction.
STRINGi3702.AT2G16570.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SI61.
SMRiQ9SI61. Positions 91-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 311221Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Transit peptide

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
InParanoidiQ9SI61.
KOiK00764.
OMAiRIIDACE.
PhylomeDBiQ9SI61.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SI61-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAATTSFSSS LSLITKPNNS SYTNQPLPLF PKPFLKPPHL SLLPSPLSSP
60 70 80 90 100
PPSLIHGVSS YFSSPSPSED NSHTPFDYHN DEDDEKPREE CGVVGIYGDP
110 120 130 140 150
EASRLCYLAL HALQHRGQEG AGIVTVSPEK VLQTITGVGL VSEVFNESKL
160 170 180 190 200
DQLPGEFAIG HVRYSTAGAS MLKNVQPFVA GYRFGSIGVA HNGNLVNYKT
210 220 230 240 250
LRAMLEENGS IFNTSSDTEV VLHLIAISKA RPFFMRIIDA CEKLQGAYSM
260 270 280 290 300
VFVTEDKLVA VRDPYGFRPL VMGRRSNGAV VFASETCALD LIEATYEREV
310 320 330 340 350
YPGEVLVVDK DGVKSQCLMP KFEPKQCIFE HIYFSLPNSI VFGRSVYESR
360 370 380 390 400
HVFGEILATE SPVECDVVIA VPDSGVVAAL GYAAKSGVPF QQGLIRSHYV
410 420 430 440 450
GRTFIEPSQK IRDFGVKLKL SPVRGVLEGK RVVVVDDSIV RGTTSSKIVR
460 470 480 490 500
LLREAGAKEV HMRIASPPIV ASCYYGVDTP SSEELISNRL SVEEINEFIG
510 520 530 540 550
SDSLAFLSFD TLKKHLGKDS KSFCYACFTG DYPVKPTEVK VKRGGGDFID
560
DGLVGSFENI EAGWVR
Length:566
Mass (Da):61,842
Last modified:May 1, 2000 - v1
Checksum:i887827A9FEB901E0
GO

Sequence cautioni

The sequence BAA06023.1 differs from that shown. Reason: Frameshift at position 70.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741T → A in BAA06023. (PubMed:7948903)Curated
Sequence conflicti106 – 1061C → F in BAA06023. (PubMed:7948903)Curated
Sequence conflicti160 – 1601G → A in BAA06023. (PubMed:7948903)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D28868 mRNA. Translation: BAA06023.1. Frameshift.
AC007195 Genomic DNA. Translation: AAD26498.1.
CP002685 Genomic DNA. Translation: AEC06510.1.
PIRiE84541.
S52622.
RefSeqiNP_179247.1. NM_127208.1.
UniGeneiAt.14491.

Genome annotation databases

EnsemblPlantsiAT2G16570.1; AT2G16570.1; AT2G16570.
GeneIDi816156.
KEGGiath:AT2G16570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D28868 mRNA. Translation: BAA06023.1 . Frameshift.
AC007195 Genomic DNA. Translation: AAD26498.1 .
CP002685 Genomic DNA. Translation: AEC06510.1 .
PIRi E84541.
S52622.
RefSeqi NP_179247.1. NM_127208.1.
UniGenei At.14491.

3D structure databases

ProteinModelPortali Q9SI61.
SMRi Q9SI61. Positions 91-535.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9SI61. 1 interaction.
STRINGi 3702.AT2G16570.1-P.

Protein family/group databases

MEROPSi C44.A01.

Proteomic databases

PRIDEi Q9SI61.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G16570.1 ; AT2G16570.1 ; AT2G16570 .
GeneIDi 816156.
KEGGi ath:AT2G16570.

Organism-specific databases

GeneFarmi 2372. 186.
TAIRi AT2G16570.

Phylogenomic databases

eggNOGi COG0034.
HOGENOMi HOG000033688.
InParanoidi Q9SI61.
KOi K00764.
OMAi RIIDACE.
PhylomeDBi Q9SI61.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci ARA:AT2G16570-MONOMER.

Gene expression databases

Genevestigatori Q9SI61.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPi MF_01931. PurF.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two amidophosphoribosyltransferase genes of Arabidopsis thaliana expressed in different organs."
    Ito T., Shiraishi H., Okada K., Shimura Y.
    Plant Mol. Biol. 26:529-533(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
    Tissue: Flower and Root.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Characterization of Arabidopsis glutamine phosphoribosyl pyrophosphate amidotransferase-deficient mutants."
    Hung W.-F., Chen L.-J., Boldt R., Sun C.-W., Li H.-M.
    Plant Physiol. 135:1314-1323(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiASE1_ARATH
AccessioniPrimary (citable) accession number: Q9SI61
Secondary accession number(s): Q38999
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3