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Protein

WRKY transcription factor 1

Gene

WRKY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor. Binds to a 5'-CGTTGACCGAG-3' consensus core sequence which contains a W box, a frequently occurring elicitor-responsive cis-acting element.

Cofactori

Zn2+Note: Metal ions. Zinc is the most probable. Required for its binding activity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi105 – 169WRKY 1PROSITE-ProRule annotationAdd BLAST65
DNA bindingi301 – 366WRKY 2PROSITE-ProRule annotationAdd BLAST66

GO - Molecular functioni

  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR
  • zinc ion binding Source: TAIR

GO - Biological processi

  • positive regulation of transcription, DNA-templated Source: TAIR
  • salicylic acid mediated signaling pathway Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
WRKY transcription factor 1
Alternative name(s):
Transcription factor ZAP1
WRKY DNA-binding protein 1
Zinc-dependent activator protein 1
Gene namesi
Name:WRKY1
Synonyms:ZAP1
Ordered Locus Names:At2g04880
ORF Names:F1O13.1, F28I8.34
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G04880.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001336441 – 487WRKY transcription factor 1Add BLAST487

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei76PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SI37.

PTM databases

iPTMnetiQ9SI37.

Expressioni

Tissue specificityi

Expressed to similar levels in root and flower, to a somewhat lower level in stem and to low levels in leaf and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ9SI37. baseline and differential.
GenevisibleiQ9SI37. AT.

Interactioni

Protein-protein interaction databases

BioGridi436. 1 interactor.
MINTiMINT-8059523.
STRINGi3702.AT2G04880.1.

Structurei

Secondary structure

1487
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi294 – 300Combined sources7
Beta strandi302 – 304Combined sources3
Beta strandi312 – 318Combined sources7
Beta strandi327 – 332Combined sources6
Beta strandi340 – 345Combined sources6
Beta strandi347 – 349Combined sources3
Beta strandi352 – 359Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AYDX-ray1.60A293-368[»]
ProteinModelPortaliQ9SI37.
SMRiQ9SI37.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9SI37.

Family & Domainsi

Sequence similaritiesi

Belongs to the WRKY group I family.Curated
Contains 2 WRKY DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410JAFU. Eukaryota.
ENOG4111DPQ. LUCA.
HOGENOMiHOG000154863.
InParanoidiQ9SI37.
KOiK18834.
OMAiCTHPNCK.
OrthoDBiEOG09360BX3.
PhylomeDBiQ9SI37.

Family and domain databases

Gene3Di2.20.25.80. 2 hits.
InterProiIPR003657. WRKY_dom.
[Graphical view]
PfamiPF03106. WRKY. 2 hits.
[Graphical view]
SMARTiSM00774. WRKY. 2 hits.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 2 hits.
PROSITEiPS50811. WRKY. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SI37-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEVGKVLAS DMELDHSNET KAVDDVVATT DKAEVIPVAV TRTETVVESL
60 70 80 90 100
ESTDCKELEK LVPHTVASQS EVDVASPVSE KAPKVSESSG ALSLQSGSEG
110 120 130 140 150
NSPFIREKVM EDGYNWRKYG QKLVKGNEFV RSYYRCTHPN CKAKKQLERS
160 170 180 190 200
AGGQVVDTVY FGEHDHPKPL AGAVPINQDK RSDVFTAVSK GEQRIDIVSL
210 220 230 240 250
IYKLCIVSYD IMFVEKTSGS SVQTLRQTEP PKIHGGLHVS VIPPADDVKT
260 270 280 290 300
DISQSSRITG DNTHKDYNSP TAKRRKKGGN IELSPVERST NDSRIVVHTQ
310 320 330 340 350
TLFDIVNDGY RWRKYGQKSV KGSPYPRSYY RCSSPGCPVK KHVERSSHDT
360 370 380 390 400
KLLITTYEGK HDHDMPPGRV VTHNNMLDSE VDDKEGDANK TPQSSTLQSI
410 420 430 440 450
TKDQHVEDHL RKKTKTNGFE KSLDQGPVLD EKLKEEIKER SDANKDHAAN
460 470 480
HAKPEAKSDD KTTVCQEKAV GTLESEEQKP KTEPAQS
Length:487
Mass (Da):54,010
Last modified:May 1, 2000 - v1
Checksum:i61721DB016897C38
GO
Isoform 2 (identifier: Q9SI37-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     191-214: Missing.

Show »
Length:463
Mass (Da):51,210
Checksum:i63C807695AD01818
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007124191 – 214Missing in isoform 2. 2 PublicationsAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92976 mRNA. Translation: CAA63554.1.
AF442389 mRNA. Translation: AAL35282.1.
AF442390 mRNA. Translation: AAL35283.1.
AC006955 Genomic DNA. Translation: AAM15341.1.
AC007211 Genomic DNA. Translation: AAD25579.1.
CP002685 Genomic DNA. Translation: AEC05880.1.
CP002685 Genomic DNA. Translation: AEC05881.1.
BT029167 mRNA. Translation: ABJ17102.1.
PIRiF84462.
RefSeqiNP_178565.1. NM_126520.4. [Q9SI37-1]
NP_849936.1. NM_179605.3. [Q9SI37-2]
UniGeneiAt.48419.
At.71110.

Genome annotation databases

EnsemblPlantsiAT2G04880.1; AT2G04880.1; AT2G04880. [Q9SI37-1]
GeneIDi815035.
GrameneiAT2G04880.1; AT2G04880.1; AT2G04880.
KEGGiath:AT2G04880.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92976 mRNA. Translation: CAA63554.1.
AF442389 mRNA. Translation: AAL35282.1.
AF442390 mRNA. Translation: AAL35283.1.
AC006955 Genomic DNA. Translation: AAM15341.1.
AC007211 Genomic DNA. Translation: AAD25579.1.
CP002685 Genomic DNA. Translation: AEC05880.1.
CP002685 Genomic DNA. Translation: AEC05881.1.
BT029167 mRNA. Translation: ABJ17102.1.
PIRiF84462.
RefSeqiNP_178565.1. NM_126520.4. [Q9SI37-1]
NP_849936.1. NM_179605.3. [Q9SI37-2]
UniGeneiAt.48419.
At.71110.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AYDX-ray1.60A293-368[»]
ProteinModelPortaliQ9SI37.
SMRiQ9SI37.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi436. 1 interactor.
MINTiMINT-8059523.
STRINGi3702.AT2G04880.1.

PTM databases

iPTMnetiQ9SI37.

Proteomic databases

PaxDbiQ9SI37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G04880.1; AT2G04880.1; AT2G04880. [Q9SI37-1]
GeneIDi815035.
GrameneiAT2G04880.1; AT2G04880.1; AT2G04880.
KEGGiath:AT2G04880.

Organism-specific databases

TAIRiAT2G04880.

Phylogenomic databases

eggNOGiENOG410JAFU. Eukaryota.
ENOG4111DPQ. LUCA.
HOGENOMiHOG000154863.
InParanoidiQ9SI37.
KOiK18834.
OMAiCTHPNCK.
OrthoDBiEOG09360BX3.
PhylomeDBiQ9SI37.

Miscellaneous databases

EvolutionaryTraceiQ9SI37.
PROiQ9SI37.

Gene expression databases

ExpressionAtlasiQ9SI37. baseline and differential.
GenevisibleiQ9SI37. AT.

Family and domain databases

Gene3Di2.20.25.80. 2 hits.
InterProiIPR003657. WRKY_dom.
[Graphical view]
PfamiPF03106. WRKY. 2 hits.
[Graphical view]
SMARTiSM00774. WRKY. 2 hits.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 2 hits.
PROSITEiPS50811. WRKY. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWRKY1_ARATH
AccessioniPrimary (citable) accession number: Q9SI37
Secondary accession number(s): Q058Q1, Q43388
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Binding to target DNA is mediated mainly by the C-terminal WRKY domain, while part of the activation domain is located between positions 210 and 285.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.