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Protein

WRKY transcription factor 1

Gene

WRKY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor. Binds to a 5'-CGTTGACCGAG-3' consensus core sequence which contains a W box, a frequently occurring elicitor-responsive cis-acting element.

Cofactori

Zn2+Note: Metal ions. Zinc is the most probable. Required for its binding activity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi105 – 16965WRKY 1PROSITE-ProRule annotationAdd
BLAST
DNA bindingi301 – 36666WRKY 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • sequence-specific DNA binding Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: TAIR
  • zinc ion binding Source: TAIR

GO - Biological processi

  • positive regulation of transcription, DNA-templated Source: TAIR
  • salicylic acid mediated signaling pathway Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
WRKY transcription factor 1
Alternative name(s):
Transcription factor ZAP1
WRKY DNA-binding protein 1
Zinc-dependent activator protein 1
Gene namesi
Name:WRKY1
Synonyms:ZAP1
Ordered Locus Names:At2g04880
ORF Names:F1O13.1, F28I8.34
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G04880.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487WRKY transcription factor 1PRO_0000133644Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei76 – 761PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SI37.
PRIDEiQ9SI37.

PTM databases

iPTMnetiQ9SI37.

Expressioni

Tissue specificityi

Expressed to similar levels in root and flower, to a somewhat lower level in stem and to low levels in leaf and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ9SI37. baseline and differential.
GenevisibleiQ9SI37. AT.

Interactioni

Protein-protein interaction databases

BioGridi436. 1 interaction.
MINTiMINT-8059523.
STRINGi3702.AT2G04880.1.

Structurei

Secondary structure

1
487
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi294 – 3007Combined sources
Beta strandi302 – 3043Combined sources
Beta strandi312 – 3187Combined sources
Beta strandi327 – 3326Combined sources
Beta strandi340 – 3456Combined sources
Beta strandi347 – 3493Combined sources
Beta strandi352 – 3598Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AYDX-ray1.60A293-368[»]
ProteinModelPortaliQ9SI37.
SMRiQ9SI37. Positions 109-169, 293-368.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9SI37.

Family & Domainsi

Sequence similaritiesi

Belongs to the WRKY group I family.Curated
Contains 2 WRKY DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410JAFU. Eukaryota.
ENOG4111DPQ. LUCA.
HOGENOMiHOG000154863.
InParanoidiQ9SI37.
KOiK18834.
OMAiCTHPNCK.
PhylomeDBiQ9SI37.

Family and domain databases

Gene3Di2.20.25.80. 2 hits.
InterProiIPR003657. WRKY_dom.
[Graphical view]
PfamiPF03106. WRKY. 2 hits.
[Graphical view]
SMARTiSM00774. WRKY. 2 hits.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 2 hits.
PROSITEiPS50811. WRKY. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SI37-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEVGKVLAS DMELDHSNET KAVDDVVATT DKAEVIPVAV TRTETVVESL
60 70 80 90 100
ESTDCKELEK LVPHTVASQS EVDVASPVSE KAPKVSESSG ALSLQSGSEG
110 120 130 140 150
NSPFIREKVM EDGYNWRKYG QKLVKGNEFV RSYYRCTHPN CKAKKQLERS
160 170 180 190 200
AGGQVVDTVY FGEHDHPKPL AGAVPINQDK RSDVFTAVSK GEQRIDIVSL
210 220 230 240 250
IYKLCIVSYD IMFVEKTSGS SVQTLRQTEP PKIHGGLHVS VIPPADDVKT
260 270 280 290 300
DISQSSRITG DNTHKDYNSP TAKRRKKGGN IELSPVERST NDSRIVVHTQ
310 320 330 340 350
TLFDIVNDGY RWRKYGQKSV KGSPYPRSYY RCSSPGCPVK KHVERSSHDT
360 370 380 390 400
KLLITTYEGK HDHDMPPGRV VTHNNMLDSE VDDKEGDANK TPQSSTLQSI
410 420 430 440 450
TKDQHVEDHL RKKTKTNGFE KSLDQGPVLD EKLKEEIKER SDANKDHAAN
460 470 480
HAKPEAKSDD KTTVCQEKAV GTLESEEQKP KTEPAQS
Length:487
Mass (Da):54,010
Last modified:May 1, 2000 - v1
Checksum:i61721DB016897C38
GO
Isoform 2 (identifier: Q9SI37-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     191-214: Missing.

Show »
Length:463
Mass (Da):51,210
Checksum:i63C807695AD01818
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei191 – 21424Missing in isoform 2. 2 PublicationsVSP_007124Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92976 mRNA. Translation: CAA63554.1.
AF442389 mRNA. Translation: AAL35282.1.
AF442390 mRNA. Translation: AAL35283.1.
AC006955 Genomic DNA. Translation: AAM15341.1.
AC007211 Genomic DNA. Translation: AAD25579.1.
CP002685 Genomic DNA. Translation: AEC05880.1.
CP002685 Genomic DNA. Translation: AEC05881.1.
BT029167 mRNA. Translation: ABJ17102.1.
PIRiF84462.
RefSeqiNP_178565.1. NM_126520.4. [Q9SI37-1]
NP_849936.1. NM_179605.2. [Q9SI37-2]
UniGeneiAt.48419.
At.71110.

Genome annotation databases

EnsemblPlantsiAT2G04880.1; AT2G04880.1; AT2G04880. [Q9SI37-1]
GeneIDi815035.
KEGGiath:AT2G04880.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92976 mRNA. Translation: CAA63554.1.
AF442389 mRNA. Translation: AAL35282.1.
AF442390 mRNA. Translation: AAL35283.1.
AC006955 Genomic DNA. Translation: AAM15341.1.
AC007211 Genomic DNA. Translation: AAD25579.1.
CP002685 Genomic DNA. Translation: AEC05880.1.
CP002685 Genomic DNA. Translation: AEC05881.1.
BT029167 mRNA. Translation: ABJ17102.1.
PIRiF84462.
RefSeqiNP_178565.1. NM_126520.4. [Q9SI37-1]
NP_849936.1. NM_179605.2. [Q9SI37-2]
UniGeneiAt.48419.
At.71110.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AYDX-ray1.60A293-368[»]
ProteinModelPortaliQ9SI37.
SMRiQ9SI37. Positions 109-169, 293-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi436. 1 interaction.
MINTiMINT-8059523.
STRINGi3702.AT2G04880.1.

PTM databases

iPTMnetiQ9SI37.

Proteomic databases

PaxDbiQ9SI37.
PRIDEiQ9SI37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G04880.1; AT2G04880.1; AT2G04880. [Q9SI37-1]
GeneIDi815035.
KEGGiath:AT2G04880.

Organism-specific databases

TAIRiAT2G04880.

Phylogenomic databases

eggNOGiENOG410JAFU. Eukaryota.
ENOG4111DPQ. LUCA.
HOGENOMiHOG000154863.
InParanoidiQ9SI37.
KOiK18834.
OMAiCTHPNCK.
PhylomeDBiQ9SI37.

Miscellaneous databases

EvolutionaryTraceiQ9SI37.
PROiQ9SI37.

Gene expression databases

ExpressionAtlasiQ9SI37. baseline and differential.
GenevisibleiQ9SI37. AT.

Family and domain databases

Gene3Di2.20.25.80. 2 hits.
InterProiIPR003657. WRKY_dom.
[Graphical view]
PfamiPF03106. WRKY. 2 hits.
[Graphical view]
SMARTiSM00774. WRKY. 2 hits.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 2 hits.
PROSITEiPS50811. WRKY. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a zinc-dependent transcriptional activator from Arabidopsis."
    De Pater S., Greco V., Pham K., Memelink J., Kijne J.
    Nucleic Acids Res. 24:4624-4631(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DNA-BINDING.
    Strain: cv. C24.
    Tissue: Flower and Silique.
  2. "Arabidopsis thaliana transcription factor WRKY1."
    Ulker B., Kushnir S., Somssich I.E.
    Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
    Tissue: Flower.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clone."
    Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  6. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiWRKY1_ARATH
AccessioniPrimary (citable) accession number: Q9SI37
Secondary accession number(s): Q058Q1, Q43388
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: May 1, 2000
Last modified: January 20, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Binding to target DNA is mediated mainly by the C-terminal WRKY domain, while part of the activation domain is located between positions 210 and 285.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.