Q9SI18 (PPA11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Purple acid phosphatase 11
EC=3.1.3.2

Gene names

Name:	PAP11
Synonyms:	AT8
Ordered Locus Names:	At2g18130
ORF Names:	F8D23.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Specifically expressed in flowers. Ref.6
Induction	By phosphate deprivation. Ref.1
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acid phosphatase activity Inferred from sequence or structural similarity Ref.1Ref.6. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 441	417	Purple acid phosphatase 11		PRO_0000372816
Regions
	Region	332 – 334	3	Substrate binding By similarity
Sites
	Active site	305	1	Proton donor By similarity
	Metal binding	145	1	Iron By similarity
	Metal binding	173	1	Iron By similarity
	Metal binding	173	1	Zinc By similarity
	Metal binding	176	1	Iron By similarity
	Metal binding	210	1	Zinc By similarity
	Metal binding	295	1	Zinc By similarity
	Metal binding	332	1	Zinc By similarity
	Metal binding	334	1	Iron By similarity
	Binding site	210	1	Substrate By similarity
Amino acid modifications
	Glycosylation	111	1	N-linked (GlcNAc...) Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	283	1	N-linked (GlcNAc...) Potential
	Glycosylation	348	1	N-linked (GlcNAc...) Potential
	Glycosylation	405	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	319	1	W → M in AAP81218. Ref.5
	Sequence conflict	324	1	K → I in AAP81218. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9SI18 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 861E402A9A936424	FASTA	441	50,362
	10 20 30 40 50 60 MELSHLALVC AAIAFSSIFV VSQAGITSTH ARVSEPSEEM SLETFPPPAG YNAPEQVHIT 70 80 90 100 110 120 QGDNAGRAMI ISWVMPLNED GSNVVTYWIA SSDGSDNKNA IATTSSYRYF NYTSGYLHHA 130 140 150 160 170 180 TIKKLEYDPS KSRSRCSLHI RYYSDLGQTY ASNQTLYNYM SNPKGQAVLF VGDLSYADDH 190 200 210 220 230 240 PNHDQRKWDS YGRFVEPSAA YQPWSWAAGN YEIDYAQSIS ETQPFKPYKN RYHVPYKASQ 250 260 270 280 290 300 STSPLWYSIK RASTYIIVLS SYSAYDKYTP QNSWLQDELK KVNRSETSWL IVLVHAPWYN 310 320 330 340 350 360 SNNYHYMEGE SMRVTFEPWF VENKVDIVFA GHVHAYERSK RISNIHYNIT DGMSTPVKDQ 370 380 390 400 410 420 NAPIYITIGD GGNIEGIANS FTDPQPSYSA FREASFGHAL LEIKNRTHAH YTWHRNKEDE 430 440 AVIADSIWLK KRYYLPEEET A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, GENE FAMILY, NOMENCLATURE. Strain: cv. Col-1.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Identification of differentially displayed Arabidopsis thaliana acid phosphatase-encoding genes." Lohrasebi T., Malboobi M.A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 241-400. Tissue: Seedling.
[6]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF492663 mRNA. Translation: AAM15912.1. AC007212 Genomic DNA. Translation: AAD31353.1. CP002685 Genomic DNA. Translation: AEC06729.1. BT026132 mRNA. Translation: ABG48488.1. AY297745 mRNA. Translation: AAP81218.1.
IPI	IPI00545420.
PIR	F84560.
RefSeq	NP_179405.1. NM_127370.1.
UniGene	At.40038.
3D structure databases
HSSP	HSSP built from PDB template 4KBP based on UniProtKB P80366.
ProteinModelPortal	Q9SI18.
SMR	Q9SI18. Positions 43-439.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G18130.1; AT2G18130.1; AT2G18130.
GeneID	816326.
KEGG	ath:AT2G18130.
Organism-specific databases
TAIR	At2g18130.
Phylogenomic databases
eggNOG	COG1409.
HOGENOM	HOG000238330.
InParanoid	Q9SI18.
PhylomeDB	Q9SI18.
ProtClustDB	CLSN2683029.
Gene expression databases
Genevestigator	Q9SI18.
Family and domain databases
Gene3D	2.60.40.380. 1 hit.
InterPro	IPR004843. Metallo_PEstase_dom. IPR008963. Purple_acid_Pase-like_N. IPR015914. Purple_acid_Pase_N. IPR025733. Purple_acid_PPase_C_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. PF14008. Metallophos_C. 1 hit. [Graphical view]
SUPFAM	SSF49363. Purple_Pase_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPA11_ARATH

Accession

Primary (citable) accession number: Q9SI18
Secondary accession number(s): Q7XY10

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program