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Q9SHS7

- BSL3_ARATH

UniProt

Q9SHS7 - BSL3_ARATH

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Protein

Serine/threonine-protein phosphatase BSL3

Gene

BSL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi709 – 7091Manganese 1By similarity
Metal bindingi711 – 7111Manganese 1By similarity
Metal bindingi743 – 7431Manganese 1By similarity
Metal bindingi743 – 7431Manganese 2By similarity
Metal bindingi775 – 7751Manganese 2By similarity
Active sitei776 – 7761Proton donorBy similarity
Metal bindingi828 – 8281Manganese 2By similarity
Metal bindingi907 – 9071Manganese 2By similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. manganese ion binding Source: InterPro
  3. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G27210-MONOMER.
ARA:GQT-317-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase BSL3 (EC:3.1.3.16)
Alternative name(s):
BSU1-like protein 3
Gene namesi
Name:BSL3
Ordered Locus Names:At2g27210
ORF Names:T22O13.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G27210.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: TAIR
  2. nucleus Source: UniProtKB-KW
  3. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10061006Serine/threonine-protein phosphatase BSL3PRO_0000058907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei616 – 6161PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9SHS7.

Expressioni

Tissue specificityi

Expressed throughout the plant, with a higher level in younger parts.1 Publication

Gene expression databases

ExpressionAtlasiQ9SHS7. baseline.
GenevestigatoriQ9SHS7.

Structurei

3D structure databases

ProteinModelPortaliQ9SHS7.
SMRiQ9SHS7. Positions 655-957.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati138 – 18447Kelch 1Add
BLAST
Repeati242 – 29049Kelch 2Add
BLAST
Repeati295 – 34551Kelch 3Add
BLAST
Repeati351 – 39848Kelch 4Add
BLAST
Repeati419 – 46547Kelch 5Add
BLAST

Sequence similaritiesi

Belongs to the PPP phosphatase family. BSU subfamily.Curated
Contains 5 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

HOGENOMiHOG000246464.
InParanoidiQ9SHS7.
OMAiTENAMVP.
PhylomeDBiQ9SHS7.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view]
PfamiPF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SHS7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLDSSMVPE NDQDPIATSE NQSPMEEKEE ASEQQTGSES ESASLTPSLP
60 70 80 90 100
PPSQQQQQQQ QQPQVTAVVG PRCAPTYSVV NAIIEKKEDG PGPRCGHTLT
110 120 130 140 150
AVPAVGEEGT SSYIGPRLIL FGGATALEGN SGGTGTPTSA GSAGIRLAGA
160 170 180 190 200
TADVHCYDVL SNKWSRLTPY GEPPSPRAAH VATAVGTMVV IQGGIGPAGL
210 220 230 240 250
SAEDLHVLDL TQQRPRWHRV VVQGPGPGPR YGHVMALVGQ RYLMAIGGND
260 270 280 290 300
GKRPLADVWA LDTAAKPYEW RKLEPEGEGP PPCMYATASA RSDGLLLLCG
310 320 330 340 350
GRDANSVPLA SAYGLAKHRD GRWEWAIAPG VSPSARYQHA AVFVNARLHV
360 370 380 390 400
SGGALGGGRM VEDSSSVAVL DTAAGVWCDT KSVVTSPRTG RYSADAAGGD
410 420 430 440 450
ASVELTRRCR HAAAAVGDLI FIYGGLRGGV LLDDLLVAED LAAAETTSAA
460 470 480 490 500
SHAAAAAAAT NTPPGRSPGR YGFSDERTGE LPESAPDAVV LGSPVAPPVN
510 520 530 540 550
GDMYTDISTE NAMVPGIRRT SKGVEYLVEA SAAEAEAISA TLAAAKARQV
560 570 580 590 600
NGEVELPDRD RGAEATPSGK PSLSLIKPDS AVPNSVIPAG VRLHHRAVVV
610 620 630 640 650
AAETGGALGG MVRQLSIDQF ENEGRRVSYG TPESATAARK LLDRQMSINS
660 670 680 690 700
VPKKVVAHLL KPRGWKPPVR RQFFLDCNEI ADLCDSAERI FSSEPTVLQL
710 720 730 740 750
KAPIKIFGDL HGQFGDLMRL FDEYGSPSTA GDISYIDYLF LGDYVDRGQH
760 770 780 790 800
SLETITLLLA LKVEYQHNVH LIRGNHEAAD INALFGFRIE CIERMGERDG
810 820 830 840 850
IWVWHRINRL FNWLPLAALI EKKIICMHGG IGRSINHVEQ IENIQRPITM
860 870 880 890 900
EAGSIVLMDL LWSDPTENDS VEGLRPNARG PGLVTFGPDR VMEFCNNNDL
910 920 930 940 950
QLIVRAHECV MDGFERFAQG HLITLFSATN YCGTANNAGA ILVLGRDLVV
960 970 980 990 1000
VPKLIHPLPP AITSPETSPE RHIEDTWMQE LNVNRPPTPT RGRPQNPNDR

GSLAWI

Note: Constructed according to the conserved gene model. No experimental confirmation available.

Length:1,006
Mass (Da):107,523
Last modified:October 23, 2007 - v2
Checksum:iE855AC8655715F64
GO
Isoform 2 (identifier: Q9SHS7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     655-707: VVAHLLKPRG...LQLKAPIKIF → NVSSQANLLC...ETYHTSITSS
     708-1006: Missing.

Note: No experimental confirmation available.

Show »
Length:707
Mass (Da):73,747
Checksum:iA1175DA6B9FC16FC
GO

Sequence cautioni

The sequence AAD26883.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei655 – 70753VVAHL…PIKIF → NVSSQANLLCYSLKLLLRYL VICMASLGISCAFLMNMVHH QQLETYHTSITSS in isoform 2. 1 PublicationVSP_028729Add
BLAST
Alternative sequencei708 – 1006299Missing in isoform 2. 1 PublicationVSP_028730Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007290 Genomic DNA. Translation: AAD26883.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07953.1.
AK229113 mRNA. Translation: BAF00990.1.
PIRiB84670.
RefSeqiNP_180289.3. NM_128279.3. [Q9SHS7-1]
UniGeneiAt.14572.

Genome annotation databases

EnsemblPlantsiAT2G27210.1; AT2G27210.1; AT2G27210. [Q9SHS7-1]
GeneIDi817263.
KEGGiath:AT2G27210.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007290 Genomic DNA. Translation: AAD26883.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC07953.1 .
AK229113 mRNA. Translation: BAF00990.1 .
PIRi B84670.
RefSeqi NP_180289.3. NM_128279.3. [Q9SHS7-1 ]
UniGenei At.14572.

3D structure databases

ProteinModelPortali Q9SHS7.
SMRi Q9SHS7. Positions 655-957.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9SHS7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G27210.1 ; AT2G27210.1 ; AT2G27210 . [Q9SHS7-1 ]
GeneIDi 817263.
KEGGi ath:AT2G27210.

Organism-specific databases

TAIRi AT2G27210.

Phylogenomic databases

HOGENOMi HOG000246464.
InParanoidi Q9SHS7.
OMAi TENAMVP.
PhylomeDBi Q9SHS7.

Enzyme and pathway databases

BioCyci ARA:AT2G27210-MONOMER.
ARA:GQT-317-MONOMER.

Gene expression databases

ExpressionAtlasi Q9SHS7. baseline.
Genevestigatori Q9SHS7.

Family and domain databases

Gene3Di 2.120.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view ]
Pfami PF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PIRSFi PIRSF036363. PPP_BSU1. 1 hit.
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis."
    Mora-Garcia S., Vert G., Yin Y., Cano-Delgado A., Cheong H., Chory J.
    Genes Dev. 18:448-460(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Root.
  7. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiBSL3_ARATH
AccessioniPrimary (citable) accession number: Q9SHS7
Secondary accession number(s): Q0WPF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: October 23, 2007
Last modified: November 26, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3