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Q9SHS7

- BSL3_ARATH

UniProt

Q9SHS7 - BSL3_ARATH

Protein

Serine/threonine-protein phosphatase BSL3

Gene

BSL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi709 – 7091Manganese 1By similarity
    Metal bindingi711 – 7111Manganese 1By similarity
    Metal bindingi743 – 7431Manganese 1By similarity
    Metal bindingi743 – 7431Manganese 2By similarity
    Metal bindingi775 – 7751Manganese 2By similarity
    Active sitei776 – 7761Proton donorBy similarity
    Metal bindingi828 – 8281Manganese 2By similarity
    Metal bindingi907 – 9071Manganese 2By similarity

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. manganese ion binding Source: InterPro
    3. phosphoprotein phosphatase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G27210-MONOMER.
    ARA:GQT-317-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase BSL3 (EC:3.1.3.16)
    Alternative name(s):
    BSU1-like protein 3
    Gene namesi
    Name:BSL3
    Ordered Locus Names:At2g27210
    ORF Names:T22O13.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G27210.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. nucleus Source: UniProtKB-SubCell
    3. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10061006Serine/threonine-protein phosphatase BSL3PRO_0000058907Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei616 – 6161PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9SHS7.

    Expressioni

    Tissue specificityi

    Expressed throughout the plant, with a higher level in younger parts.1 Publication

    Gene expression databases

    GenevestigatoriQ9SHS7.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SHS7.
    SMRiQ9SHS7. Positions 150-439, 614-966.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati138 – 18447Kelch 1Add
    BLAST
    Repeati242 – 29049Kelch 2Add
    BLAST
    Repeati295 – 34551Kelch 3Add
    BLAST
    Repeati351 – 39848Kelch 4Add
    BLAST
    Repeati419 – 46547Kelch 5Add
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. BSU subfamily.Curated
    Contains 5 Kelch repeats.Curated

    Keywords - Domaini

    Kelch repeat, Repeat

    Phylogenomic databases

    HOGENOMiHOG000246464.
    InParanoidiQ9SHS7.
    OMAiTENAMVP.
    PhylomeDBiQ9SHS7.

    Family and domain databases

    Gene3Di2.120.10.80. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR015915. Kelch-typ_b-propeller.
    IPR011498. Kelch_2.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    IPR012391. Ser/Thr_prot_Pase_BSU1.
    [Graphical view]
    PfamiPF07646. Kelch_2. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9SHS7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDLDSSMVPE NDQDPIATSE NQSPMEEKEE ASEQQTGSES ESASLTPSLP     50
    PPSQQQQQQQ QQPQVTAVVG PRCAPTYSVV NAIIEKKEDG PGPRCGHTLT 100
    AVPAVGEEGT SSYIGPRLIL FGGATALEGN SGGTGTPTSA GSAGIRLAGA 150
    TADVHCYDVL SNKWSRLTPY GEPPSPRAAH VATAVGTMVV IQGGIGPAGL 200
    SAEDLHVLDL TQQRPRWHRV VVQGPGPGPR YGHVMALVGQ RYLMAIGGND 250
    GKRPLADVWA LDTAAKPYEW RKLEPEGEGP PPCMYATASA RSDGLLLLCG 300
    GRDANSVPLA SAYGLAKHRD GRWEWAIAPG VSPSARYQHA AVFVNARLHV 350
    SGGALGGGRM VEDSSSVAVL DTAAGVWCDT KSVVTSPRTG RYSADAAGGD 400
    ASVELTRRCR HAAAAVGDLI FIYGGLRGGV LLDDLLVAED LAAAETTSAA 450
    SHAAAAAAAT NTPPGRSPGR YGFSDERTGE LPESAPDAVV LGSPVAPPVN 500
    GDMYTDISTE NAMVPGIRRT SKGVEYLVEA SAAEAEAISA TLAAAKARQV 550
    NGEVELPDRD RGAEATPSGK PSLSLIKPDS AVPNSVIPAG VRLHHRAVVV 600
    AAETGGALGG MVRQLSIDQF ENEGRRVSYG TPESATAARK LLDRQMSINS 650
    VPKKVVAHLL KPRGWKPPVR RQFFLDCNEI ADLCDSAERI FSSEPTVLQL 700
    KAPIKIFGDL HGQFGDLMRL FDEYGSPSTA GDISYIDYLF LGDYVDRGQH 750
    SLETITLLLA LKVEYQHNVH LIRGNHEAAD INALFGFRIE CIERMGERDG 800
    IWVWHRINRL FNWLPLAALI EKKIICMHGG IGRSINHVEQ IENIQRPITM 850
    EAGSIVLMDL LWSDPTENDS VEGLRPNARG PGLVTFGPDR VMEFCNNNDL 900
    QLIVRAHECV MDGFERFAQG HLITLFSATN YCGTANNAGA ILVLGRDLVV 950
    VPKLIHPLPP AITSPETSPE RHIEDTWMQE LNVNRPPTPT RGRPQNPNDR 1000
    GSLAWI 1006

    Note: Constructed according to the conserved gene model. No experimental confirmation available.

    Length:1,006
    Mass (Da):107,523
    Last modified:October 23, 2007 - v2
    Checksum:iE855AC8655715F64
    GO
    Isoform 2 (identifier: Q9SHS7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         655-707: VVAHLLKPRG...LQLKAPIKIF → NVSSQANLLC...ETYHTSITSS
         708-1006: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:707
    Mass (Da):73,747
    Checksum:iA1175DA6B9FC16FC
    GO

    Sequence cautioni

    The sequence AAD26883.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei655 – 70753VVAHL…PIKIF → NVSSQANLLCYSLKLLLRYL VICMASLGISCAFLMNMVHH QQLETYHTSITSS in isoform 2. 1 PublicationVSP_028729Add
    BLAST
    Alternative sequencei708 – 1006299Missing in isoform 2. 1 PublicationVSP_028730Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007290 Genomic DNA. Translation: AAD26883.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC07953.1.
    AK229113 mRNA. Translation: BAF00990.1.
    PIRiB84670.
    RefSeqiNP_180289.3. NM_128279.3. [Q9SHS7-1]
    UniGeneiAt.14572.

    Genome annotation databases

    EnsemblPlantsiAT2G27210.1; AT2G27210.1; AT2G27210. [Q9SHS7-1]
    GeneIDi817263.
    KEGGiath:AT2G27210.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007290 Genomic DNA. Translation: AAD26883.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC07953.1 .
    AK229113 mRNA. Translation: BAF00990.1 .
    PIRi B84670.
    RefSeqi NP_180289.3. NM_128279.3. [Q9SHS7-1 ]
    UniGenei At.14572.

    3D structure databases

    ProteinModelPortali Q9SHS7.
    SMRi Q9SHS7. Positions 150-439, 614-966.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9SHS7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G27210.1 ; AT2G27210.1 ; AT2G27210 . [Q9SHS7-1 ]
    GeneIDi 817263.
    KEGGi ath:AT2G27210.

    Organism-specific databases

    TAIRi AT2G27210.

    Phylogenomic databases

    HOGENOMi HOG000246464.
    InParanoidi Q9SHS7.
    OMAi TENAMVP.
    PhylomeDBi Q9SHS7.

    Enzyme and pathway databases

    BioCyci ARA:AT2G27210-MONOMER.
    ARA:GQT-317-MONOMER.

    Gene expression databases

    Genevestigatori Q9SHS7.

    Family and domain databases

    Gene3Di 2.120.10.80. 1 hit.
    3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR015915. Kelch-typ_b-propeller.
    IPR011498. Kelch_2.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    IPR012391. Ser/Thr_prot_Pase_BSU1.
    [Graphical view ]
    Pfami PF07646. Kelch_2. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036363. PPP_BSU1. 1 hit.
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    4. "Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis."
      Mora-Garcia S., Vert G., Yin Y., Cano-Delgado A., Cheong H., Chory J.
      Genes Dev. 18:448-460(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    5. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Root.
    7. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiBSL3_ARATH
    AccessioniPrimary (citable) accession number: Q9SHS7
    Secondary accession number(s): Q0WPF9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2004
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3