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Q9SHS7

- BSL3_ARATH

UniProt

Q9SHS7 - BSL3_ARATH

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Protein
Serine/threonine-protein phosphatase BSL3
Gene
BSL3, At2g27210, T22O13.2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi709 – 7091Manganese 1 By similarity
Metal bindingi711 – 7111Manganese 1 By similarity
Metal bindingi743 – 7431Manganese 1 By similarity
Metal bindingi743 – 7431Manganese 2 By similarity
Metal bindingi775 – 7751Manganese 2 By similarity
Active sitei776 – 7761Proton donor By similarity
Metal bindingi828 – 8281Manganese 2 By similarity
Metal bindingi907 – 9071Manganese 2 By similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. manganese ion binding Source: InterPro
  3. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G27210-MONOMER.
ARA:GQT-317-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase BSL3 (EC:3.1.3.16)
Alternative name(s):
BSU1-like protein 3
Gene namesi
Name:BSL3
Ordered Locus Names:At2g27210
ORF Names:T22O13.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G27210.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: TAIR
  2. nucleus Source: UniProtKB-SubCell
  3. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10061006Serine/threonine-protein phosphatase BSL3
PRO_0000058907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei616 – 6161Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9SHS7.

Expressioni

Tissue specificityi

Expressed throughout the plant, with a higher level in younger parts.1 Publication

Gene expression databases

GenevestigatoriQ9SHS7.

Structurei

3D structure databases

ProteinModelPortaliQ9SHS7.
SMRiQ9SHS7. Positions 150-439, 614-966.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati138 – 18447Kelch 1
Add
BLAST
Repeati242 – 29049Kelch 2
Add
BLAST
Repeati295 – 34551Kelch 3
Add
BLAST
Repeati351 – 39848Kelch 4
Add
BLAST
Repeati419 – 46547Kelch 5
Add
BLAST

Sequence similaritiesi

Contains 5 Kelch repeats.

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

HOGENOMiHOG000246464.
InParanoidiQ9SHS7.
OMAiTENAMVP.
PhylomeDBiQ9SHS7.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view]
PfamiPF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SHS7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDLDSSMVPE NDQDPIATSE NQSPMEEKEE ASEQQTGSES ESASLTPSLP     50
PPSQQQQQQQ QQPQVTAVVG PRCAPTYSVV NAIIEKKEDG PGPRCGHTLT 100
AVPAVGEEGT SSYIGPRLIL FGGATALEGN SGGTGTPTSA GSAGIRLAGA 150
TADVHCYDVL SNKWSRLTPY GEPPSPRAAH VATAVGTMVV IQGGIGPAGL 200
SAEDLHVLDL TQQRPRWHRV VVQGPGPGPR YGHVMALVGQ RYLMAIGGND 250
GKRPLADVWA LDTAAKPYEW RKLEPEGEGP PPCMYATASA RSDGLLLLCG 300
GRDANSVPLA SAYGLAKHRD GRWEWAIAPG VSPSARYQHA AVFVNARLHV 350
SGGALGGGRM VEDSSSVAVL DTAAGVWCDT KSVVTSPRTG RYSADAAGGD 400
ASVELTRRCR HAAAAVGDLI FIYGGLRGGV LLDDLLVAED LAAAETTSAA 450
SHAAAAAAAT NTPPGRSPGR YGFSDERTGE LPESAPDAVV LGSPVAPPVN 500
GDMYTDISTE NAMVPGIRRT SKGVEYLVEA SAAEAEAISA TLAAAKARQV 550
NGEVELPDRD RGAEATPSGK PSLSLIKPDS AVPNSVIPAG VRLHHRAVVV 600
AAETGGALGG MVRQLSIDQF ENEGRRVSYG TPESATAARK LLDRQMSINS 650
VPKKVVAHLL KPRGWKPPVR RQFFLDCNEI ADLCDSAERI FSSEPTVLQL 700
KAPIKIFGDL HGQFGDLMRL FDEYGSPSTA GDISYIDYLF LGDYVDRGQH 750
SLETITLLLA LKVEYQHNVH LIRGNHEAAD INALFGFRIE CIERMGERDG 800
IWVWHRINRL FNWLPLAALI EKKIICMHGG IGRSINHVEQ IENIQRPITM 850
EAGSIVLMDL LWSDPTENDS VEGLRPNARG PGLVTFGPDR VMEFCNNNDL 900
QLIVRAHECV MDGFERFAQG HLITLFSATN YCGTANNAGA ILVLGRDLVV 950
VPKLIHPLPP AITSPETSPE RHIEDTWMQE LNVNRPPTPT RGRPQNPNDR 1000
GSLAWI 1006

Note: Constructed according to the conserved gene model. No experimental confirmation available.

Length:1,006
Mass (Da):107,523
Last modified:October 23, 2007 - v2
Checksum:iE855AC8655715F64
GO
Isoform 2 (identifier: Q9SHS7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     655-707: VVAHLLKPRG...LQLKAPIKIF → NVSSQANLLC...ETYHTSITSS
     708-1006: Missing.

Note: No experimental confirmation available.

Show »
Length:707
Mass (Da):73,747
Checksum:iA1175DA6B9FC16FC
GO

Sequence cautioni

The sequence AAD26883.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei655 – 70753VVAHL…PIKIF → NVSSQANLLCYSLKLLLRYL VICMASLGISCAFLMNMVHH QQLETYHTSITSS in isoform 2.
VSP_028729Add
BLAST
Alternative sequencei708 – 1006299Missing in isoform 2.
VSP_028730Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007290 Genomic DNA. Translation: AAD26883.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07953.1.
AK229113 mRNA. Translation: BAF00990.1.
PIRiB84670.
RefSeqiNP_180289.3. NM_128279.3. [Q9SHS7-1]
UniGeneiAt.14572.

Genome annotation databases

EnsemblPlantsiAT2G27210.1; AT2G27210.1; AT2G27210. [Q9SHS7-1]
GeneIDi817263.
KEGGiath:AT2G27210.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007290 Genomic DNA. Translation: AAD26883.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC07953.1 .
AK229113 mRNA. Translation: BAF00990.1 .
PIRi B84670.
RefSeqi NP_180289.3. NM_128279.3. [Q9SHS7-1 ]
UniGenei At.14572.

3D structure databases

ProteinModelPortali Q9SHS7.
SMRi Q9SHS7. Positions 150-439, 614-966.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9SHS7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G27210.1 ; AT2G27210.1 ; AT2G27210 . [Q9SHS7-1 ]
GeneIDi 817263.
KEGGi ath:AT2G27210.

Organism-specific databases

TAIRi AT2G27210.

Phylogenomic databases

HOGENOMi HOG000246464.
InParanoidi Q9SHS7.
OMAi TENAMVP.
PhylomeDBi Q9SHS7.

Enzyme and pathway databases

BioCyci ARA:AT2G27210-MONOMER.
ARA:GQT-317-MONOMER.

Gene expression databases

Genevestigatori Q9SHS7.

Family and domain databases

Gene3Di 2.120.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view ]
Pfami PF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PIRSFi PIRSF036363. PPP_BSU1. 1 hit.
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis."
    Mora-Garcia S., Vert G., Yin Y., Cano-Delgado A., Cheong H., Chory J.
    Genes Dev. 18:448-460(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Root.
  7. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiBSL3_ARATH
AccessioniPrimary (citable) accession number: Q9SHS7
Secondary accession number(s): Q0WPF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: October 23, 2007
Last modified: June 11, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi