Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase BSL3

Gene

BSL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi709Manganese 1By similarity1
Metal bindingi711Manganese 1By similarity1
Metal bindingi743Manganese 1By similarity1
Metal bindingi743Manganese 2By similarity1
Metal bindingi775Manganese 2By similarity1
Active sitei776Proton donorBy similarity1
Metal bindingi828Manganese 2By similarity1
Metal bindingi907Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase BSL3 (EC:3.1.3.16)
Alternative name(s):
BSU1-like protein 3
Gene namesi
Name:BSL3
Ordered Locus Names:At2g27210
ORF Names:T22O13.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G27210.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000589071 – 1006Serine/threonine-protein phosphatase BSL3Add BLAST1006

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei616PhosphoserineBy similarity1
Modified residuei964PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SHS7.
PRIDEiQ9SHS7.

PTM databases

iPTMnetiQ9SHS7.

Expressioni

Tissue specificityi

Expressed throughout the plant, with a higher level in younger parts.1 Publication

Gene expression databases

ExpressionAtlasiQ9SHS7. differential.
GenevisibleiQ9SHS7. AT.

Interactioni

Protein-protein interaction databases

BioGridi2615. 1 interactor.
STRINGi3702.AT2G27210.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SHS7.
SMRiQ9SHS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati138 – 184Kelch 1Add BLAST47
Repeati242 – 290Kelch 2Add BLAST49
Repeati295 – 345Kelch 3Add BLAST51
Repeati351 – 398Kelch 4Add BLAST48
Repeati419 – 465Kelch 5Add BLAST47

Sequence similaritiesi

Belongs to the PPP phosphatase family. BSU subfamily.Curated
Contains 5 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
KOG0379. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000246464.
InParanoidiQ9SHS7.
OMAiMVPENDQ.
OrthoDBiEOG093601IG.
PhylomeDBiQ9SHS7.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view]
PfamiPF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SHS7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLDSSMVPE NDQDPIATSE NQSPMEEKEE ASEQQTGSES ESASLTPSLP
60 70 80 90 100
PPSQQQQQQQ QQPQVTAVVG PRCAPTYSVV NAIIEKKEDG PGPRCGHTLT
110 120 130 140 150
AVPAVGEEGT SSYIGPRLIL FGGATALEGN SGGTGTPTSA GSAGIRLAGA
160 170 180 190 200
TADVHCYDVL SNKWSRLTPY GEPPSPRAAH VATAVGTMVV IQGGIGPAGL
210 220 230 240 250
SAEDLHVLDL TQQRPRWHRV VVQGPGPGPR YGHVMALVGQ RYLMAIGGND
260 270 280 290 300
GKRPLADVWA LDTAAKPYEW RKLEPEGEGP PPCMYATASA RSDGLLLLCG
310 320 330 340 350
GRDANSVPLA SAYGLAKHRD GRWEWAIAPG VSPSARYQHA AVFVNARLHV
360 370 380 390 400
SGGALGGGRM VEDSSSVAVL DTAAGVWCDT KSVVTSPRTG RYSADAAGGD
410 420 430 440 450
ASVELTRRCR HAAAAVGDLI FIYGGLRGGV LLDDLLVAED LAAAETTSAA
460 470 480 490 500
SHAAAAAAAT NTPPGRSPGR YGFSDERTGE LPESAPDAVV LGSPVAPPVN
510 520 530 540 550
GDMYTDISTE NAMVPGIRRT SKGVEYLVEA SAAEAEAISA TLAAAKARQV
560 570 580 590 600
NGEVELPDRD RGAEATPSGK PSLSLIKPDS AVPNSVIPAG VRLHHRAVVV
610 620 630 640 650
AAETGGALGG MVRQLSIDQF ENEGRRVSYG TPESATAARK LLDRQMSINS
660 670 680 690 700
VPKKVVAHLL KPRGWKPPVR RQFFLDCNEI ADLCDSAERI FSSEPTVLQL
710 720 730 740 750
KAPIKIFGDL HGQFGDLMRL FDEYGSPSTA GDISYIDYLF LGDYVDRGQH
760 770 780 790 800
SLETITLLLA LKVEYQHNVH LIRGNHEAAD INALFGFRIE CIERMGERDG
810 820 830 840 850
IWVWHRINRL FNWLPLAALI EKKIICMHGG IGRSINHVEQ IENIQRPITM
860 870 880 890 900
EAGSIVLMDL LWSDPTENDS VEGLRPNARG PGLVTFGPDR VMEFCNNNDL
910 920 930 940 950
QLIVRAHECV MDGFERFAQG HLITLFSATN YCGTANNAGA ILVLGRDLVV
960 970 980 990 1000
VPKLIHPLPP AITSPETSPE RHIEDTWMQE LNVNRPPTPT RGRPQNPNDR

GSLAWI
Note: Constructed according to the conserved gene model. No experimental confirmation available.
Length:1,006
Mass (Da):107,523
Last modified:October 23, 2007 - v2
Checksum:iE855AC8655715F64
GO
Isoform 2 (identifier: Q9SHS7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     655-707: VVAHLLKPRG...LQLKAPIKIF → NVSSQANLLC...ETYHTSITSS
     708-1006: Missing.

Note: No experimental confirmation available.
Show »
Length:707
Mass (Da):73,747
Checksum:iA1175DA6B9FC16FC
GO

Sequence cautioni

The sequence AAD26883 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028729655 – 707VVAHL…PIKIF → NVSSQANLLCYSLKLLLRYL VICMASLGISCAFLMNMVHH QQLETYHTSITSS in isoform 2. 1 PublicationAdd BLAST53
Alternative sequenceiVSP_028730708 – 1006Missing in isoform 2. 1 PublicationAdd BLAST299

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007290 Genomic DNA. Translation: AAD26883.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07953.1.
AK229113 mRNA. Translation: BAF00990.1.
PIRiB84670.
RefSeqiNP_180289.3. NM_128279.4. [Q9SHS7-1]
UniGeneiAt.14572.

Genome annotation databases

EnsemblPlantsiAT2G27210.1; AT2G27210.1; AT2G27210. [Q9SHS7-1]
GeneIDi817263.
GrameneiAT2G27210.1; AT2G27210.1; AT2G27210.
KEGGiath:AT2G27210.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007290 Genomic DNA. Translation: AAD26883.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07953.1.
AK229113 mRNA. Translation: BAF00990.1.
PIRiB84670.
RefSeqiNP_180289.3. NM_128279.4. [Q9SHS7-1]
UniGeneiAt.14572.

3D structure databases

ProteinModelPortaliQ9SHS7.
SMRiQ9SHS7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2615. 1 interactor.
STRINGi3702.AT2G27210.1.

PTM databases

iPTMnetiQ9SHS7.

Proteomic databases

PaxDbiQ9SHS7.
PRIDEiQ9SHS7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G27210.1; AT2G27210.1; AT2G27210. [Q9SHS7-1]
GeneIDi817263.
GrameneiAT2G27210.1; AT2G27210.1; AT2G27210.
KEGGiath:AT2G27210.

Organism-specific databases

TAIRiAT2G27210.

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
KOG0379. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000246464.
InParanoidiQ9SHS7.
OMAiMVPENDQ.
OrthoDBiEOG093601IG.
PhylomeDBiQ9SHS7.

Miscellaneous databases

PROiQ9SHS7.

Gene expression databases

ExpressionAtlasiQ9SHS7. differential.
GenevisibleiQ9SHS7. AT.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view]
PfamiPF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBSL3_ARATH
AccessioniPrimary (citable) accession number: Q9SHS7
Secondary accession number(s): Q0WPF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: October 23, 2007
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.