Q9SHS7 (BSL3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase BSL3
EC=3.1.3.16

Alternative name(s):
BSU1-like protein 3

Gene names

Name:	BSL3
Ordered Locus Names:	At2g27210
ORF Names:	T22O13.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1006 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling. Ref.4
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subcellular location	Nucleus.
Tissue specificity	Expressed throughout the plant, with a higher level in younger parts. Ref.4
Sequence similarities	Belongs to the PPP phosphatase family. BSU subfamily. Contains 5 Kelch repeats.
Sequence caution	The sequence AAD26883.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Kelch repeat Repeat
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from direct assay PubMed 18433157. Source: TAIR nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	iron ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9SHS7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Constructed according to the conserved gene model. No experimental confirmation available.

Isoform 2 (identifier: Q9SHS7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     655-707: VVAHLLKPRG...LQLKAPIKIF → NVSSQANLLC...ETYHTSITSS
     708-1006: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1006	1006	Serine/threonine-protein phosphatase BSL3		PRO_0000058907
Regions
	Repeat	138 – 184	47	Kelch 1
	Repeat	242 – 290	49	Kelch 2
	Repeat	295 – 345	51	Kelch 3
	Repeat	351 – 398	48	Kelch 4
	Repeat	419 – 465	47	Kelch 5
Sites
	Active site	776	1	Proton donor By similarity
	Metal binding	709	1	Iron By similarity
	Metal binding	711	1	Iron By similarity
	Metal binding	743	1	Iron By similarity
	Metal binding	743	1	Manganese By similarity
	Metal binding	775	1	Manganese By similarity
	Metal binding	828	1	Manganese By similarity
	Metal binding	907	1	Manganese By similarity
Amino acid modifications
	Modified residue	616	1	Phosphoserine Ref.6
	Modified residue	964	1	Phosphoserine Ref.7
	Modified residue	968	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	655 – 707	53	VVAHL…PIKIF → NVSSQANLLCYSLKLLLRYL VICMASLGISCAFLMNMVHH QQLETYHTSITSS in isoform 2.		VSP_028729
	Alternative sequence	708 – 1006	299	Missing in isoform 2.		VSP_028730

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 23, 2007. Version 2. Checksum: E855AC8655715F64	FASTA	1,006	107,523
	10 20 30 40 50 60 MDLDSSMVPE NDQDPIATSE NQSPMEEKEE ASEQQTGSES ESASLTPSLP PPSQQQQQQQ 70 80 90 100 110 120 QQPQVTAVVG PRCAPTYSVV NAIIEKKEDG PGPRCGHTLT AVPAVGEEGT SSYIGPRLIL 130 140 150 160 170 180 FGGATALEGN SGGTGTPTSA GSAGIRLAGA TADVHCYDVL SNKWSRLTPY GEPPSPRAAH 190 200 210 220 230 240 VATAVGTMVV IQGGIGPAGL SAEDLHVLDL TQQRPRWHRV VVQGPGPGPR YGHVMALVGQ 250 260 270 280 290 300 RYLMAIGGND GKRPLADVWA LDTAAKPYEW RKLEPEGEGP PPCMYATASA RSDGLLLLCG 310 320 330 340 350 360 GRDANSVPLA SAYGLAKHRD GRWEWAIAPG VSPSARYQHA AVFVNARLHV SGGALGGGRM 370 380 390 400 410 420 VEDSSSVAVL DTAAGVWCDT KSVVTSPRTG RYSADAAGGD ASVELTRRCR HAAAAVGDLI 430 440 450 460 470 480 FIYGGLRGGV LLDDLLVAED LAAAETTSAA SHAAAAAAAT NTPPGRSPGR YGFSDERTGE 490 500 510 520 530 540 LPESAPDAVV LGSPVAPPVN GDMYTDISTE NAMVPGIRRT SKGVEYLVEA SAAEAEAISA 550 560 570 580 590 600 TLAAAKARQV NGEVELPDRD RGAEATPSGK PSLSLIKPDS AVPNSVIPAG VRLHHRAVVV 610 620 630 640 650 660 AAETGGALGG MVRQLSIDQF ENEGRRVSYG TPESATAARK LLDRQMSINS VPKKVVAHLL 670 680 690 700 710 720 KPRGWKPPVR RQFFLDCNEI ADLCDSAERI FSSEPTVLQL KAPIKIFGDL HGQFGDLMRL 730 740 750 760 770 780 FDEYGSPSTA GDISYIDYLF LGDYVDRGQH SLETITLLLA LKVEYQHNVH LIRGNHEAAD 790 800 810 820 830 840 INALFGFRIE CIERMGERDG IWVWHRINRL FNWLPLAALI EKKIICMHGG IGRSINHVEQ 850 860 870 880 890 900 IENIQRPITM EAGSIVLMDL LWSDPTENDS VEGLRPNARG PGLVTFGPDR VMEFCNNNDL 910 920 930 940 950 960 QLIVRAHECV MDGFERFAQG HLITLFSATN YCGTANNAGA ILVLGRDLVV VPKLIHPLPP 970 980 990 1000 AITSPETSPE RHIEDTWMQE LNVNRPPTPT RGRPQNPNDR GSLAWI
	Isoform 2 [UniParc]. Checksum: A1175DA6B9FC16FC	FASTA	707	73,747
	10 20 30 40 50 60 MDLDSSMVPE NDQDPIATSE NQSPMEEKEE ASEQQTGSES ESASLTPSLP PPSQQQQQQQ 70 80 90 100 110 120 QQPQVTAVVG PRCAPTYSVV NAIIEKKEDG PGPRCGHTLT AVPAVGEEGT SSYIGPRLIL 130 140 150 160 170 180 FGGATALEGN SGGTGTPTSA GSAGIRLAGA TADVHCYDVL SNKWSRLTPY GEPPSPRAAH 190 200 210 220 230 240 VATAVGTMVV IQGGIGPAGL SAEDLHVLDL TQQRPRWHRV VVQGPGPGPR YGHVMALVGQ 250 260 270 280 290 300 RYLMAIGGND GKRPLADVWA LDTAAKPYEW RKLEPEGEGP PPCMYATASA RSDGLLLLCG 310 320 330 340 350 360 GRDANSVPLA SAYGLAKHRD GRWEWAIAPG VSPSARYQHA AVFVNARLHV SGGALGGGRM 370 380 390 400 410 420 VEDSSSVAVL DTAAGVWCDT KSVVTSPRTG RYSADAAGGD ASVELTRRCR HAAAAVGDLI 430 440 450 460 470 480 FIYGGLRGGV LLDDLLVAED LAAAETTSAA SHAAAAAAAT NTPPGRSPGR YGFSDERTGE 490 500 510 520 530 540 LPESAPDAVV LGSPVAPPVN GDMYTDISTE NAMVPGIRRT SKGVEYLVEA SAAEAEAISA 550 560 570 580 590 600 TLAAAKARQV NGEVELPDRD RGAEATPSGK PSLSLIKPDS AVPNSVIPAG VRLHHRAVVV 610 620 630 640 650 660 AAETGGALGG MVRQLSIDQF ENEGRRVSYG TPESATAARK LLDRQMSINS VPKKNVSSQA 670 680 690 700 NLLCYSLKLL LRYLVICMAS LGISCAFLMN MVHHQQLETY HTSITSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[4]	"Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis." Mora-Garcia S., Vert G., Yin Y., Cano-Delgado A., Cheong H., Chory J. Genes Dev. 18:448-460(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	"Arabidopsis PPP family of serine/threonine phosphatases." Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C. Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana." Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C. J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-616, SUBCELLULAR LOCATION, MASS SPECTROMETRY. Strain: cv. Columbia.
[7]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-964 AND SER-968, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007290 Genomic DNA. Translation: AAD26883.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC07953.1. AK229113 mRNA. Translation: BAF00990.1.
IPI	IPI00786022. IPI00870927.
PIR	B84670.
RefSeq	NP_180289.3. NM_128279.3.
UniGene	At.14572.
3D structure databases
ProteinModelPortal	Q9SHS7.
SMR	Q9SHS7. Positions 655-957.
ModBase	Search...
Proteomic databases
PRIDE	Q9SHS7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G27210.1; AT2G27210.1; AT2G27210.
GeneID	817263.
KEGG	ath:AT2G27210.
Organism-specific databases
TAIR	At2g27210.
Phylogenomic databases
HOGENOM	HOG000246464.
InParanoid	Q9SHS7.
OMA	TENAMVP.
PhylomeDB	Q9SHS7.
ProtClustDB	CLSN2682801.
Gene expression databases
ArrayExpress	Q9SHS7.
Genevestigator	Q9SHS7.
GermOnline	AT2G27210. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.120.10.80. 1 hit.
InterPro	IPR015915. Kelch-typ_b-propeller. IPR011498. Kelch_2. IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. IPR012391. Ser/Thr_prot_Pase_BSU1. [Graphical view]
Pfam	PF07646. Kelch_2. 1 hit. PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF036363. PPP_BSU1. 1 hit.
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BSL3_ARATH

Accession

Primary (citable) accession number: Q9SHS7
Secondary accession number(s): Q0WPF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	October 23, 2007
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program