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Q9SHS7 (BSL3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase BSL3

EC=3.1.3.16
Alternative name(s):
BSU1-like protein 3
Gene names
Name:BSL3
Ordered Locus Names:At2g27210
ORF Names:T22O13.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1006 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling. Ref.4

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Subcellular location

Nucleus Ref.7.

Tissue specificity

Expressed throughout the plant, with a higher level in younger parts. Ref.4

Sequence similarities

Belongs to the PPP phosphatase family. BSU subfamily.

Contains 5 Kelch repeats.

Sequence caution

The sequence AAD26883.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9SHS7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Constructed according to the conserved gene model. No experimental confirmation available.
Isoform 2 (identifier: Q9SHS7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     655-707: VVAHLLKPRG...LQLKAPIKIF → NVSSQANLLC...ETYHTSITSS
     708-1006: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10061006Serine/threonine-protein phosphatase BSL3
PRO_0000058907

Regions

Repeat138 – 18447Kelch 1
Repeat242 – 29049Kelch 2
Repeat295 – 34551Kelch 3
Repeat351 – 39848Kelch 4
Repeat419 – 46547Kelch 5

Sites

Active site7761Proton donor By similarity
Metal binding7091Iron By similarity
Metal binding7111Iron By similarity
Metal binding7431Iron By similarity
Metal binding7431Manganese By similarity
Metal binding7751Manganese By similarity
Metal binding8281Manganese By similarity
Metal binding9071Manganese By similarity

Amino acid modifications

Modified residue6161Phosphoserine By similarity

Natural variations

Alternative sequence655 – 70753VVAHL…PIKIF → NVSSQANLLCYSLKLLLRYL VICMASLGISCAFLMNMVHH QQLETYHTSITSS in isoform 2.
VSP_028729
Alternative sequence708 – 1006299Missing in isoform 2.
VSP_028730

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 23, 2007. Version 2.
Checksum: E855AC8655715F64

FASTA1,006107,523
        10         20         30         40         50         60 
MDLDSSMVPE NDQDPIATSE NQSPMEEKEE ASEQQTGSES ESASLTPSLP PPSQQQQQQQ 

        70         80         90        100        110        120 
QQPQVTAVVG PRCAPTYSVV NAIIEKKEDG PGPRCGHTLT AVPAVGEEGT SSYIGPRLIL 

       130        140        150        160        170        180 
FGGATALEGN SGGTGTPTSA GSAGIRLAGA TADVHCYDVL SNKWSRLTPY GEPPSPRAAH 

       190        200        210        220        230        240 
VATAVGTMVV IQGGIGPAGL SAEDLHVLDL TQQRPRWHRV VVQGPGPGPR YGHVMALVGQ 

       250        260        270        280        290        300 
RYLMAIGGND GKRPLADVWA LDTAAKPYEW RKLEPEGEGP PPCMYATASA RSDGLLLLCG 

       310        320        330        340        350        360 
GRDANSVPLA SAYGLAKHRD GRWEWAIAPG VSPSARYQHA AVFVNARLHV SGGALGGGRM 

       370        380        390        400        410        420 
VEDSSSVAVL DTAAGVWCDT KSVVTSPRTG RYSADAAGGD ASVELTRRCR HAAAAVGDLI 

       430        440        450        460        470        480 
FIYGGLRGGV LLDDLLVAED LAAAETTSAA SHAAAAAAAT NTPPGRSPGR YGFSDERTGE 

       490        500        510        520        530        540 
LPESAPDAVV LGSPVAPPVN GDMYTDISTE NAMVPGIRRT SKGVEYLVEA SAAEAEAISA 

       550        560        570        580        590        600 
TLAAAKARQV NGEVELPDRD RGAEATPSGK PSLSLIKPDS AVPNSVIPAG VRLHHRAVVV 

       610        620        630        640        650        660 
AAETGGALGG MVRQLSIDQF ENEGRRVSYG TPESATAARK LLDRQMSINS VPKKVVAHLL 

       670        680        690        700        710        720 
KPRGWKPPVR RQFFLDCNEI ADLCDSAERI FSSEPTVLQL KAPIKIFGDL HGQFGDLMRL 

       730        740        750        760        770        780 
FDEYGSPSTA GDISYIDYLF LGDYVDRGQH SLETITLLLA LKVEYQHNVH LIRGNHEAAD 

       790        800        810        820        830        840 
INALFGFRIE CIERMGERDG IWVWHRINRL FNWLPLAALI EKKIICMHGG IGRSINHVEQ 

       850        860        870        880        890        900 
IENIQRPITM EAGSIVLMDL LWSDPTENDS VEGLRPNARG PGLVTFGPDR VMEFCNNNDL 

       910        920        930        940        950        960 
QLIVRAHECV MDGFERFAQG HLITLFSATN YCGTANNAGA ILVLGRDLVV VPKLIHPLPP 

       970        980        990       1000 
AITSPETSPE RHIEDTWMQE LNVNRPPTPT RGRPQNPNDR GSLAWI 

« Hide

Isoform 2 [UniParc].

Checksum: A1175DA6B9FC16FC
Show »

FASTA70773,747

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis."
Mora-Garcia S., Vert G., Yin Y., Cano-Delgado A., Cheong H., Chory J.
Genes Dev. 18:448-460(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]"Arabidopsis PPP family of serine/threonine phosphatases."
Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis."
de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H.
J. Proteome Res. 7:2458-2470(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Root.
[7]"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC007290 Genomic DNA. Translation: AAD26883.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07953.1.
AK229113 mRNA. Translation: BAF00990.1.
PIRB84670.
RefSeqNP_180289.3. NM_128279.3.
UniGeneAt.14572.

3D structure databases

ProteinModelPortalQ9SHS7.
SMRQ9SHS7. Positions 150-439, 614-966.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9SHS7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G27210.1; AT2G27210.1; AT2G27210. [Q9SHS7-1]
GeneID817263.
KEGGath:AT2G27210.

Organism-specific databases

TAIRAT2G27210.

Phylogenomic databases

HOGENOMHOG000246464.
InParanoidQ9SHS7.
OMATENAMVP.
PhylomeDBQ9SHS7.
ProtClustDBCLSN2682801.

Enzyme and pathway databases

BioCycARA:AT2G27210-MONOMER.
ARA:GQT-317-MONOMER.

Gene expression databases

GenevestigatorQ9SHS7.

Family and domain databases

Gene3D2.120.10.80. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
[Graphical view]
PfamPF07646. Kelch_2. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFPIRSF036363. PPP_BSU1. 1 hit.
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBSL3_ARATH
AccessionPrimary (citable) accession number: Q9SHS7
Secondary accession number(s): Q0WPF9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: October 23, 2007
Last modified: April 16, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names