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Q9SHJ3 (CALS7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Callose synthase 7
EC=2.4.1.34
Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 7
Gene names
Name:CALS7
Synonyms:GSL7
Ordered Locus Names:At1g06490
ORF Names:F12K11.17
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length1958 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in callose synthesis at the forming cell plate during cytokinesis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals By similarity.

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Sequence caution

The sequence AAF24822.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19581958Callose synthase 7
PRO_0000334579

Regions

Topological domain1 – 504504Cytoplasmic Potential
Transmembrane505 – 52521Helical; Potential
Topological domain526 – 53510Extracellular Potential
Transmembrane536 – 55621Helical; Potential
Topological domain557 – 56913Cytoplasmic Potential
Transmembrane570 – 59021Helical; Potential
Topological domain591 – 62030Extracellular Potential
Transmembrane621 – 64121Helical; Potential
Topological domain642 – 67332Cytoplasmic Potential
Transmembrane674 – 69421Helical; Potential
Topological domain695 – 73036Extracellular Potential
Transmembrane731 – 75121Helical; Potential
Topological domain752 – 1496745Cytoplasmic Potential
Transmembrane1497 – 151721Helical; Potential
Topological domain1518 – 154730Extracellular Potential
Transmembrane1548 – 156821Helical; Potential
Topological domain1569 – 15768Cytoplasmic Potential
Transmembrane1577 – 159721Helical; Potential
Topological domain1598 – 164043Extracellular Potential
Transmembrane1641 – 166121Helical; Potential
Topological domain1662 – 16676Cytoplasmic Potential
Transmembrane1668 – 168821Helical; Potential
Topological domain1689 – 174254Extracellular Potential
Transmembrane1743 – 176321Helical; Potential
Topological domain1764 – 17718Cytoplasmic Potential
Transmembrane1772 – 179221Helical; Potential
Topological domain1793 – 181220Extracellular Potential
Transmembrane1813 – 183321Helical; Potential
Topological domain1834 – 18352Cytoplasmic Potential
Transmembrane1836 – 185621Helical; Potential
Topological domain1857 – 187822Extracellular Potential
Transmembrane1879 – 189921Helical; Potential
Topological domain1900 – 195859Cytoplasmic Potential
Compositional bias111 – 1155Poly-Glu
Compositional bias1037 – 10415Poly-Leu

Sequences

Sequence LengthMass (Da)Tools
Q9SHJ3 [UniParc].

Last modified May 31, 2011. Version 3.
Checksum: 40AC43FFC980F692

FASTA1,958228,032
        10         20         30         40         50         60 
MASTSSGGRG EDGRPPQMQP VRSMSRKMTR AGTMMIEHPN EDERPIDSEL VPSSLASIAP 

        70         80         90        100        110        120 
ILRVANDIDQ DNARVAYLCR FHAFEKAHRM DPTSSGRGVR QFKTYLLHKL EEEEEITEHM 

       130        140        150        160        170        180 
LAKSDPREIQ LYYQTFYENN IQDGEGKKTP EEMAKLYQIA TVLYDVLKTV VPQARIDDKT 

       190        200        210        220        230        240 
LRYAKEVERK KEQYEHYNIL PLYALGAKTA VMELPEIKAA ILAVCNVDNL PRPRFHSASA 

       250        260        270        280        290        300 
NLDEVDRERG RSFNDILEWL ALVFGFQRGN VANQREHLIL LLANIDVRKR DLENYVEIKP 

       310        320        330        340        350        360 
STVRKLMEKY FKNYNSWCKY LRCDSYLRFP AGCDKQQLSL LYIGLYLLIW GEASNVRFMP 

       370        380        390        400        410        420 
ECLCYIFHNM ANEVHGILFG NVYPVTGDTY EAGAPDEEAF LRNVITPIYQ VLRKEVRRNK 

       430        440        450        460        470        480 
NGKASHSKWR NYDDLNEYFW DKRCFRLKWP MNFKADFFIH TDEISQVPNQ RHDQVSHGKR 

       490        500        510        520        530        540 
KPKTNFVEAR TFWNLYRSFD RMWMFLVLSL QTMIIVAWHP SGSILAIFTE DVFRNVLTIF 

       550        560        570        580        590        600 
ITSAFLNLLQ ATLDLVLSFG AWKSLKFSQI MRYITKFLMA AMWAIMLPIT YSKSVQNPTG 

       610        620        630        640        650        660 
LIKFFSSWVG SWLHRSLYDY AIALYVLPNI LAAVFFLLPP LRRIMERSNM RIVTLIMWWA 

       670        680        690        700        710        720 
QPKLYIGRGM HEEMFALFKY TFFWVMLLLS KLAFSYYVEI LPLVNPTKLI WDMHVVNYEW 

       730        740        750        760        770        780 
HEFFPNATHN IGVIIAIWGP IVLVYFMDTQ IWYAIFSTLF GGIYGAFSHL GEIRTLGMLR 

       790        800        810        820        830        840 
SRFKVVPSAF CSKLTPLPLG HAKRKHLDET VDEKDIARFS QMWNKFIHTM RDEDLISDRE 

       850        860        870        880        890        900 
RDLLLVPSSS GDVTVVQWPP FLLASKIPIA LDMAKDFKGK EDVDLFKKIK SEYYMHYAVV 

       910        920        930        940        950        960 
EAYETVRDII YGLLQDESDK RIVREICYEV DISIQQHRFL SEFRMTGMPL LSDKLEKFLK 

       970        980        990       1000       1010       1020 
ILLSDYEEDD YKSQIINVLQ DIIEIITQDV MVNGHEILER AHLQSGDIES DKKEQRFEKI 

      1030       1040       1050       1060       1070       1080 
DLSLTQNISW REKVVRLLLL LTVKESAINI PQSLEARRRM TFFANSLFMN MPDAPRVRDM 

      1090       1100       1110       1120       1130       1140 
LSFSVLTPYY KEDVLYSEEE LNKENEDGIT ILFYLQRIYP EEWSNYCERV NDLKRNLSEK 

      1150       1160       1170       1180       1190       1200 
DKAEQLRQWV SYRGQTLSRT VRGMMYYRVA LELQCFQEYT EENATNGGYL PSESNEDDRK 

      1210       1220       1230       1240       1250       1260 
AFSDRARALA DLKFTYVVSC QVYGNQKKSS ESRDRSCYNN ILQLMLKYPS LRVAYIDERE 

      1270       1280       1290       1300       1310       1320 
ETVNGKSQKV FYSVLLKGCD KLDEEIYRIK LPGPPTEIGE GKPENQNHAI IFTRGEALQT 

      1330       1340       1350       1360       1370       1380 
IDMNQDNYFE ECFKMRNVLQ EFDEGRRGKR NPTILGLREH IFTGSVSSLA WFMSNQETSF 

      1390       1400       1410       1420       1430       1440 
VTIGQRVLAN PLRVRFHYGH PDIFDRIFHI TRGGISKASK IINLSEDIFA GYNSTLRGGY 

      1450       1460       1470       1480       1490       1500 
VTHHEYIQAG KGRDVGMNQI SFFEAKVANG NGEQTLSRDV YRLGRRFDFY RMLSFYFTTV 

      1510       1520       1530       1540       1550       1560 
GFYFSSMITV LTVYVFLYGR LYLVLSGLEK NILQSASVHE SNALEQALAA QSVFQLGFLM 

      1570       1580       1590       1600       1610       1620 
VLPMVMEIGL EKGFRTALGD FIIMQLQLAS VFFTFQLGTK AHYFGRTILH GGSKYRATGR 

      1630       1640       1650       1660       1670       1680 
GFVVFHAKFA ENYRLYSRSH FVKGLELVIL LVVYQVYGTS YRSSSTYMYI TFSMWFLVTS 

      1690       1700       1710       1720       1730       1740 
WLFAPFIFNP SGFEWQKTVD DWTDWKRWMG NRGGIGIVLD KSWESWWDIE QEHLKHTNLR 

      1750       1760       1770       1780       1790       1800 
GRVLEILLAL RFLLYQYGIV YHLNIARRHT TFLVYGLSWA ILLSVLLVLK MVSMGRRKFG 

      1810       1820       1830       1840       1850       1860 
TDFQVMFRIL KALLFLGFLS VMTVLFVVCG LTISDLFASI LAFLPTGWAI LLIGQALRSV 

      1870       1880       1890       1900       1910       1920 
FKGLGFWDSV KELGRAYEYI MGLVIFTPIA VLSWFPFVSE FQTRLLFNQA FSRGLQISMI 

      1930       1940       1950 
LAGKKDKETP STKYLGHTEE SFGLEHDTNT FNHYYLWT

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"A cell plate-specific callose synthase and its interaction with phragmoplastin."
Hong Z., Delauney A.J., Verma D.P.S.
Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract]
Cited for: GENE FAMILY AND NOMENCLATURE.
[4]"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC007592 Genomic DNA. Translation: AAF24822.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27993.1.
IPIIPI00524232.
PIRF86200.
RefSeqNP_172136.2. NM_100528.2.
UniGeneAt.51506.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Proteomic databases

PRIDEQ9SHJ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G06490.1; AT1G06490.1; AT1G06490.
GeneID837160.
GenomeReviewsGene locus AT1G06490 in contig CT485782_GR.
KEGGath:AT1G06490.

Organism-specific databases

TAIRAt1g06490.

Phylogenomic databases

GeneTreeEPGT00070000029319.
HOGENOMHBG316390.
InParanoidQ9SHJ3.
OMAYGAFSHL.
ProtClustDBCLSN2682326.

Enzyme and pathway databases

BioCycMetaCyc:AT1G06490-MONOMER.

Gene expression databases

GenevestigatorQ9SHJ3.

Family and domain databases

InterProIPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1_N.
[Graphical view]
Gene3DG3DSA:1.25.40.270. G3DSA:1.25.40.270. 1 hit.
KOK11000.
PfamPF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCALS7_ARATH
AccessionPrimary (citable) accession number: Q9SHJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 31, 2011
Last modified: January 25, 2012
This is version 60 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents