UBC34

Functionⁱ

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityⁱ

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	87	Glycyl thioester intermediatePROSITE-ProRule annotation		1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
ubiquitin protein ligase activity Source: GO_Central
ubiquitin protein ligase binding Source: GO_Central
ubiquitin-protein transferase activity
Source: TAIR
Inferred from direct assayⁱ
- 16339806

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

protein polyubiquitination Source: GO_Central
ubiquitin-dependent ERAD pathway Source: GO_Central
ubiquitin-dependent protein catabolic process
Source: TAIR
Inferred from direct assayⁱ
- 16339806

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Ubl conjugation pathway
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayⁱ	UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin-conjugating enzyme E2 34 (EC:2.3.2.23) Alternative name(s): E2 ubiquitin-conjugating enzyme 34 Ubiquitin carrier protein 34
Gene namesⁱ	Name:UBC34 Ordered Locus Names:At1g17280 ORF Names:F20D23.1
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 1

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT1G17280
TAIRⁱ	locus:2020392 AT1G17280

Topology

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transmembraneⁱ	214 – 234	HelicalSequence analysisAdd BLAST		21

Keywords - Cellular componentⁱ

Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000345199	1 – 237	Ubiquitin-conjugating enzyme E2 34Add BLAST		237

Proteomic databases

PaxDbⁱ	Q9SHI7

PaxDbⁱ

Q9SHI7

Expressionⁱ

Gene expression databases

ExpressionAtlasⁱ	Q9SHI7 baseline and differential
Genevisibleⁱ	Q9SHI7 AT

Interactionⁱ

GO - Molecular functionⁱ

ubiquitin protein ligase binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	23540448 interactors.
IntActⁱ	Q9SHI7 1 interactor.
STRINGⁱ	3702.AT1G17280.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9SHI7
SMRⁱ	Q9SHI7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0894 Eukaryota ENOG410Z0AN LUCA
HOGENOMⁱ	HOG000170329
InParanoidⁱ	Q9SHI7
KEGG Orthology (KO) More...KOⁱ	K04554
OMAⁱ	FQPPSIY
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360KZX
PhylomeDBⁱ	Q9SHI7

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195 UBCc, 1 hit
Gene3Dⁱ	3.10.110.101 hit
InterProⁱ	View protein in InterPro IPR000608 UBQ-conjugat_E2 IPR016135 UBQ-conjugating_enzyme/RWD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179 UQ_con, 1 hit
SUPFAMⁱ	SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9SHI7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAEKACIKRL QKEYRALCKE PVSHVVARPS PNDILEWHYV LEGSEGTPFA 
        60         70         80         90        100
GGFYYGKIKF PPEYPYKPPG ITMTTPNGRF MTQKKICLSM SDFHPESWNP 
       110        120        130        140        150
MWSVSSILTG LLSFMMDTSP TTGSVNTTVI EKQRLAKSSL AFNCKTPAFR 
       160        170        180        190        200
KLFPEYVEKY NQQQLAEQAT TQLTTPESPQ KSDTKVESEK TIDPTKGDSE 
       210        220        230 
GGLKERKKNN KQGLPAWIIL LLVSVFGVVM ALPLLQL

Length:237

Mass (Da):26,614

Last modified:May 1, 2000 - v1

Checksum:ⁱFE2888F36BF8B8EC

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AC007651 Genomic DNA Translation: AAD50006.1 CP002684 Genomic DNA Translation: AEE29567.1 CP002684 Genomic DNA Translation: AEE29568.1 CP002684 Genomic DNA Translation: ANM59253.1 CP002684 Genomic DNA Translation: ANM59254.1 CP002684 Genomic DNA Translation: ANM59255.1 CP002684 Genomic DNA Translation: ANM59256.1 CP002684 Genomic DNA Translation: ANM59257.1 CP002684 Genomic DNA Translation: ANM59259.1 BT003073 mRNA Translation: AAO23638.1 AK227344 mRNA Translation: BAE99355.1 AY084676 mRNA Translation: AAM61238.1 DQ027047 mRNA Translation: AAY44873.1
PIRⁱ	C86309
NCBI Reference Sequences More...RefSeqⁱ	NP_001077554.1, NM_001084085.1 NP_001319027.1, NM_001332287.1 NP_001321626.1, NM_001332292.1 NP_001321627.1, NM_001332293.1 NP_001321628.1, NM_001332288.1 NP_001321629.1, NM_001332290.1 NP_001321630.1, NM_001332291.1 NP_173172.1, NM_101590.4
UniGeneⁱ	At.42947

Genome annotation databases

EnsemblPlantsⁱ	AT1G17280.1; AT1G17280.1; AT1G17280 AT1G17280.2; AT1G17280.2; AT1G17280 AT1G17280.3; AT1G17280.3; AT1G17280 AT1G17280.5; AT1G17280.5; AT1G17280 AT1G17280.6; AT1G17280.6; AT1G17280 AT1G17280.7; AT1G17280.7; AT1G17280 AT1G17280.8; AT1G17280.8; AT1G17280 AT1G17280.9; AT1G17280.9; AT1G17280
GeneIDⁱ	838300
Grameneⁱ	AT1G17280.1; AT1G17280.1; AT1G17280 AT1G17280.2; AT1G17280.2; AT1G17280 AT1G17280.3; AT1G17280.3; AT1G17280 AT1G17280.5; AT1G17280.5; AT1G17280 AT1G17280.6; AT1G17280.6; AT1G17280 AT1G17280.7; AT1G17280.7; AT1G17280 AT1G17280.8; AT1G17280.8; AT1G17280 AT1G17280.9; AT1G17280.9; AT1G17280
KEGGⁱ	ath:AT1G17280

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9SHI7	A0A178W1E2	Arabidopsis thaliana (Mouse-ear cress)	237	UniRef100_Q9SHI7	Cluster: Ubiquitin-conjugating enzyme E2 34	2

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9SHI7	A0A178W1E2 A0A1P8ARH3 A0A0D3DWU9 UPI0004EE073C R0IJ31 D7KFD7 UPI00053A960D UPI00053A9EF3 UPI00053B2947 V4KBK5 +8	Arabidopsis thaliana (Mouse-ear cress) Brassica oleracea var. oleracea Brassica napus (Rape) Capsella rubella Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress) Camelina sativa (False flax) (Myagrum sativum) Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum) Brassica rapa subsp. pekinensis (Chinese cabbage) (Brassica pekinensis) Raphanus sativus (Radish) Rhinolophus sinicus (Chinese rufous horseshoe bat)	237	UniRef90_Q9SHI7	Cluster: Ubiquitin-conjugating enzyme E2 34	19

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9SHI7	Q9FK29 A0A178UPT5 A0A178UPX0 A0A178URD9 R0EYB8 UPI00053AB426 UPI0005399790 UPI000A29D9A2 D7MQ79 UPI00053ADBE0 +126	Arabidopsis thaliana (Mouse-ear cress) Capsella rubella Camelina sativa (False flax) (Myagrum sativum) Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress) Klebsormidium nitens (Green alga) (Ulothrix nitens) Zostera marina (Eelgrass) Sesamum indicum (Oriental sesame) (Sesamum orientale) Musa acuminata subsp. malaccensis (Wild banana) (Musa malaccensis) Jatropha curcas (Barbados nut) Selaginella moellendorffii (Spikemoss) And more	243	UniRef50_Q9FK29	Cluster: Probable ubiquitin-conjugating enzyme E2 33	137

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AC007651 Genomic DNA Translation: AAD50006.1 CP002684 Genomic DNA Translation: AEE29567.1 CP002684 Genomic DNA Translation: AEE29568.1 CP002684 Genomic DNA Translation: ANM59253.1 CP002684 Genomic DNA Translation: ANM59254.1 CP002684 Genomic DNA Translation: ANM59255.1 CP002684 Genomic DNA Translation: ANM59256.1 CP002684 Genomic DNA Translation: ANM59257.1 CP002684 Genomic DNA Translation: ANM59259.1 BT003073 mRNA Translation: AAO23638.1 AK227344 mRNA Translation: BAE99355.1 AY084676 mRNA Translation: AAM61238.1 DQ027047 mRNA Translation: AAY44873.1
PIRⁱ	C86309
NCBI Reference Sequences More...RefSeqⁱ	NP_001077554.1, NM_001084085.1 NP_001319027.1, NM_001332287.1 NP_001321626.1, NM_001332292.1 NP_001321627.1, NM_001332293.1 NP_001321628.1, NM_001332288.1 NP_001321629.1, NM_001332290.1 NP_001321630.1, NM_001332291.1 NP_173172.1, NM_101590.4
UniGeneⁱ	At.42947

3D structure databases

ProteinModelPortalⁱ	Q9SHI7
SMRⁱ	Q9SHI7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	23540448 interactors.
IntActⁱ	Q9SHI7 1 interactor.
STRINGⁱ	3702.AT1G17280.1

Proteomic databases

PaxDbⁱ	Q9SHI7

PaxDbⁱ

Q9SHI7

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT1G17280.1; AT1G17280.1; AT1G17280 AT1G17280.2; AT1G17280.2; AT1G17280 AT1G17280.3; AT1G17280.3; AT1G17280 AT1G17280.5; AT1G17280.5; AT1G17280 AT1G17280.6; AT1G17280.6; AT1G17280 AT1G17280.7; AT1G17280.7; AT1G17280 AT1G17280.8; AT1G17280.8; AT1G17280 AT1G17280.9; AT1G17280.9; AT1G17280
GeneIDⁱ	838300
Grameneⁱ	AT1G17280.1; AT1G17280.1; AT1G17280 AT1G17280.2; AT1G17280.2; AT1G17280 AT1G17280.3; AT1G17280.3; AT1G17280 AT1G17280.5; AT1G17280.5; AT1G17280 AT1G17280.6; AT1G17280.6; AT1G17280 AT1G17280.7; AT1G17280.7; AT1G17280 AT1G17280.8; AT1G17280.8; AT1G17280 AT1G17280.9; AT1G17280.9; AT1G17280
KEGGⁱ	ath:AT1G17280

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT1G17280
TAIRⁱ	locus:2020392 AT1G17280

Phylogenomic databases

eggNOGⁱ	KOG0894 Eukaryota ENOG410Z0AN LUCA
HOGENOMⁱ	HOG000170329
InParanoidⁱ	Q9SHI7
KEGG Orthology (KO) More...KOⁱ	K04554
OMAⁱ	FQPPSIY
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360KZX
PhylomeDBⁱ	Q9SHI7

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
Reactomeⁱ	R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9SHI7

PROⁱ

PR:Q9SHI7

Gene expression databases

ExpressionAtlasⁱ	Q9SHI7 baseline and differential
Genevisibleⁱ	Q9SHI7 AT

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195 UBCc, 1 hit
Gene3Dⁱ	3.10.110.101 hit
InterProⁱ	View protein in InterPro IPR000608 UBQ-conjugat_E2 IPR016135 UBQ-conjugating_enzyme/RWD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179 UQ_con, 1 hit
SUPFAMⁱ	SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBC34_ARATH
Accessionⁱ	Q9SHI7Primary (citable) accession number: Q9SHI7 Secondary accession number(s): Q4TYX7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
	Last sequence update:	May 1, 2000
	Last modified:	April 25, 2018
	This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9SHI7 (UBC34_ARATH)

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Ubiquitin-conjugating enzyme E2 34

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Endoplasmic reticulum

Other locations

Topology

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ