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Protein

Ubiquitin-conjugating enzyme E2 34

Gene

UBC34

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G17280-MONOMER.
ARA:GQT-1971-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 34 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 34
Ubiquitin carrier protein 34
Gene namesi
Name:UBC34
Ordered Locus Names:At1g17280
ORF Names:F20D23.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G17280.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei214 – 23421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Ubiquitin-conjugating enzyme E2 34PRO_0000345199Add
BLAST

Proteomic databases

PaxDbiQ9SHI7.
PRIDEiQ9SHI7.

Expressioni

Gene expression databases

GenevisibleiQ9SHI7. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi23540. 448 interactions.
IntActiQ9SHI7. 1 interaction.
STRINGi3702.AT1G17280.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SHI7.
SMRiQ9SHI7. Positions 8-164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0894. Eukaryota.
ENOG410Z0AN. LUCA.
HOGENOMiHOG000170329.
InParanoidiQ9SHI7.
KOiK04554.
OMAiPPGISMI.
OrthoDBiEOG09360KZX.
PhylomeDBiQ9SHI7.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SHI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKACIKRL QKEYRALCKE PVSHVVARPS PNDILEWHYV LEGSEGTPFA
60 70 80 90 100
GGFYYGKIKF PPEYPYKPPG ITMTTPNGRF MTQKKICLSM SDFHPESWNP
110 120 130 140 150
MWSVSSILTG LLSFMMDTSP TTGSVNTTVI EKQRLAKSSL AFNCKTPAFR
160 170 180 190 200
KLFPEYVEKY NQQQLAEQAT TQLTTPESPQ KSDTKVESEK TIDPTKGDSE
210 220 230
GGLKERKKNN KQGLPAWIIL LLVSVFGVVM ALPLLQL
Length:237
Mass (Da):26,614
Last modified:May 1, 2000 - v1
Checksum:iFE2888F36BF8B8EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007651 Genomic DNA. Translation: AAD50006.1.
CP002684 Genomic DNA. Translation: AEE29567.1.
CP002684 Genomic DNA. Translation: AEE29568.1.
BT003073 mRNA. Translation: AAO23638.1.
AK227344 mRNA. Translation: BAE99355.1.
AY084676 mRNA. Translation: AAM61238.1.
DQ027047 mRNA. Translation: AAY44873.1.
PIRiC86309.
RefSeqiNP_001077554.1. NM_001084085.1.
NP_173172.1. NM_101590.3.
UniGeneiAt.42947.

Genome annotation databases

EnsemblPlantsiAT1G17280.1; AT1G17280.1; AT1G17280.
AT1G17280.2; AT1G17280.2; AT1G17280.
GeneIDi838300.
GrameneiAT1G17280.1; AT1G17280.1; AT1G17280.
AT1G17280.2; AT1G17280.2; AT1G17280.
KEGGiath:AT1G17280.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007651 Genomic DNA. Translation: AAD50006.1.
CP002684 Genomic DNA. Translation: AEE29567.1.
CP002684 Genomic DNA. Translation: AEE29568.1.
BT003073 mRNA. Translation: AAO23638.1.
AK227344 mRNA. Translation: BAE99355.1.
AY084676 mRNA. Translation: AAM61238.1.
DQ027047 mRNA. Translation: AAY44873.1.
PIRiC86309.
RefSeqiNP_001077554.1. NM_001084085.1.
NP_173172.1. NM_101590.3.
UniGeneiAt.42947.

3D structure databases

ProteinModelPortaliQ9SHI7.
SMRiQ9SHI7. Positions 8-164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23540. 448 interactions.
IntActiQ9SHI7. 1 interaction.
STRINGi3702.AT1G17280.1.

Proteomic databases

PaxDbiQ9SHI7.
PRIDEiQ9SHI7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G17280.1; AT1G17280.1; AT1G17280.
AT1G17280.2; AT1G17280.2; AT1G17280.
GeneIDi838300.
GrameneiAT1G17280.1; AT1G17280.1; AT1G17280.
AT1G17280.2; AT1G17280.2; AT1G17280.
KEGGiath:AT1G17280.

Organism-specific databases

TAIRiAT1G17280.

Phylogenomic databases

eggNOGiKOG0894. Eukaryota.
ENOG410Z0AN. LUCA.
HOGENOMiHOG000170329.
InParanoidiQ9SHI7.
KOiK04554.
OMAiPPGISMI.
OrthoDBiEOG09360KZX.
PhylomeDBiQ9SHI7.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:AT1G17280-MONOMER.
ARA:GQT-1971-MONOMER.

Miscellaneous databases

PROiQ9SHI7.

Gene expression databases

GenevisibleiQ9SHI7. AT.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC34_ARATH
AccessioniPrimary (citable) accession number: Q9SHI7
Secondary accession number(s): Q4TYX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.