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Protein

Ubiquitin-conjugating enzyme E2 34

Gene

UBC34

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 34 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 34
Ubiquitin carrier protein 34
Gene namesi
Name:UBC34
Ordered Locus Names:At1g17280
ORF Names:F20D23.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G17280.
TAIRilocus:2020392. AT1G17280.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451991 – 237Ubiquitin-conjugating enzyme E2 34Add BLAST237

Proteomic databases

PaxDbiQ9SHI7.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SHI7. baseline and differential.
GenevisibleiQ9SHI7. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi23540. 448 interactors.
IntActiQ9SHI7. 1 interactor.
STRINGi3702.AT1G17280.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SHI7.
SMRiQ9SHI7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0894. Eukaryota.
ENOG410Z0AN. LUCA.
HOGENOMiHOG000170329.
InParanoidiQ9SHI7.
KOiK04554.
OMAiFQPPSIY.
OrthoDBiEOG09360KZX.
PhylomeDBiQ9SHI7.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SHI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKACIKRL QKEYRALCKE PVSHVVARPS PNDILEWHYV LEGSEGTPFA
60 70 80 90 100
GGFYYGKIKF PPEYPYKPPG ITMTTPNGRF MTQKKICLSM SDFHPESWNP
110 120 130 140 150
MWSVSSILTG LLSFMMDTSP TTGSVNTTVI EKQRLAKSSL AFNCKTPAFR
160 170 180 190 200
KLFPEYVEKY NQQQLAEQAT TQLTTPESPQ KSDTKVESEK TIDPTKGDSE
210 220 230
GGLKERKKNN KQGLPAWIIL LLVSVFGVVM ALPLLQL
Length:237
Mass (Da):26,614
Last modified:May 1, 2000 - v1
Checksum:iFE2888F36BF8B8EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007651 Genomic DNA. Translation: AAD50006.1.
CP002684 Genomic DNA. Translation: AEE29567.1.
CP002684 Genomic DNA. Translation: AEE29568.1.
CP002684 Genomic DNA. Translation: ANM59253.1.
CP002684 Genomic DNA. Translation: ANM59254.1.
CP002684 Genomic DNA. Translation: ANM59255.1.
CP002684 Genomic DNA. Translation: ANM59256.1.
CP002684 Genomic DNA. Translation: ANM59257.1.
CP002684 Genomic DNA. Translation: ANM59259.1.
BT003073 mRNA. Translation: AAO23638.1.
AK227344 mRNA. Translation: BAE99355.1.
AY084676 mRNA. Translation: AAM61238.1.
DQ027047 mRNA. Translation: AAY44873.1.
PIRiC86309.
RefSeqiNP_001077554.1. NM_001084085.1.
NP_001319027.1. NM_001332287.1.
NP_001321626.1. NM_001332292.1.
NP_001321627.1. NM_001332293.1.
NP_001321628.1. NM_001332288.1.
NP_001321629.1. NM_001332290.1.
NP_001321630.1. NM_001332291.1.
NP_173172.1. NM_101590.4.
UniGeneiAt.42947.

Genome annotation databases

EnsemblPlantsiAT1G17280.1; AT1G17280.1; AT1G17280.
AT1G17280.2; AT1G17280.2; AT1G17280.
AT1G17280.3; AT1G17280.3; AT1G17280.
AT1G17280.5; AT1G17280.5; AT1G17280.
AT1G17280.6; AT1G17280.6; AT1G17280.
AT1G17280.7; AT1G17280.7; AT1G17280.
AT1G17280.8; AT1G17280.8; AT1G17280.
AT1G17280.9; AT1G17280.9; AT1G17280.
GeneIDi838300.
GrameneiAT1G17280.1; AT1G17280.1; AT1G17280.
AT1G17280.2; AT1G17280.2; AT1G17280.
AT1G17280.3; AT1G17280.3; AT1G17280.
AT1G17280.5; AT1G17280.5; AT1G17280.
AT1G17280.6; AT1G17280.6; AT1G17280.
AT1G17280.7; AT1G17280.7; AT1G17280.
AT1G17280.8; AT1G17280.8; AT1G17280.
AT1G17280.9; AT1G17280.9; AT1G17280.
KEGGiath:AT1G17280.

Similar proteinsi

Entry informationi

Entry nameiUBC34_ARATH
AccessioniPrimary (citable) accession number: Q9SHI7
Secondary accession number(s): Q4TYX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: May 1, 2000
Last modified: August 30, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families