UBC34

Functionⁱ

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityⁱ

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	87	Glycyl thioester intermediatePROSITE-ProRule annotation		1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
ubiquitin protein ligase activity Source: GO_Central
ubiquitin protein ligase binding Source: GO_Central
ubiquitin-protein transferase activity
Source: TAIR
Inferred from direct assayⁱ
- 16339806

Complete GO annotation...

GO - Biological processⁱ

protein polyubiquitination Source: GO_Central
ubiquitin-dependent ERAD pathway Source: GO_Central
ubiquitin-dependent protein catabolic process
Source: TAIR
Inferred from direct assayⁱ
- 16339806

Complete GO annotation...

Keywordsⁱ

Molecular function	Transferase
Biological process	Ubl conjugation pathway
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00143.

UniPathwayⁱ

UPA00143.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin-conjugating enzyme E2 34 (EC:2.3.2.23) Alternative name(s): E2 ubiquitin-conjugating enzyme 34 Ubiquitin carrier protein 34
Gene namesⁱ	Name:UBC34 Ordered Locus Names:At1g17280 ORF Names:F20D23.1
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 1

Organism-specific databases

TAIRⁱ	locus:2020392. AT1G17280.

TAIRⁱ

locus:2020392. AT1G17280.

Subcellular locationⁱ

Membrane Curated; Single-pass membrane protein Curated

Topology

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transmembraneⁱ	214 – 234	HelicalSequence analysisAdd BLAST		21

GO - Cellular componentⁱ

endoplasmic reticulum Source: GO_Central
integral component of membrane Source: UniProtKB-KW

Complete GO annotation...

Keywords - Cellular componentⁱ

Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000345199	1 – 237	Ubiquitin-conjugating enzyme E2 34Add BLAST		237

Proteomic databases

PaxDbⁱ	Q9SHI7.

PaxDbⁱ

Q9SHI7.

Expressionⁱ

Gene expression databases

ExpressionAtlasⁱ	Q9SHI7. baseline.
Genevisibleⁱ	Q9SHI7. AT.

Interactionⁱ

GO - Molecular functionⁱ

ubiquitin protein ligase binding Source: GO_Central

Complete GO annotation...

Protein-protein interaction databases

BioGridⁱ	23540. 448 interactors.
IntActⁱ	Q9SHI7. 1 interactor.
STRINGⁱ	3702.AT1G17280.1.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9SHI7.
SMRⁱ	Q9SHI7.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0894. Eukaryota. ENOG410Z0AN. LUCA.
HOGENOMⁱ	HOG000170329.
InParanoidⁱ	Q9SHI7.
KEGG Orthology (KO) More...KOⁱ	K04554.
OMAⁱ	FQPPSIY.
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360KZX.
PhylomeDBⁱ	Q9SHI7.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195. UBCc. 1 hit.
Gene3Dⁱ	3.10.110.10. 1 hit.
InterProⁱ	View protein in InterPro IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179. UQ_con. 1 hit.
SUPFAMⁱ	SSF54495. SSF54495. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9SHI7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAEKACIKRL QKEYRALCKE PVSHVVARPS PNDILEWHYV LEGSEGTPFA 
        60         70         80         90        100
GGFYYGKIKF PPEYPYKPPG ITMTTPNGRF MTQKKICLSM SDFHPESWNP 
       110        120        130        140        150
MWSVSSILTG LLSFMMDTSP TTGSVNTTVI EKQRLAKSSL AFNCKTPAFR 
       160        170        180        190        200
KLFPEYVEKY NQQQLAEQAT TQLTTPESPQ KSDTKVESEK TIDPTKGDSE 
       210        220        230 
GGLKERKKNN KQGLPAWIIL LLVSVFGVVM ALPLLQL

Length:237

Mass (Da):26,614

Last modified:May 1, 2000 - v1

Checksum:ⁱFE2888F36BF8B8EC

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AC007651 Genomic DNA. Translation: AAD50006.1. CP002684 Genomic DNA. Translation: AEE29567.1. CP002684 Genomic DNA. Translation: AEE29568.1. CP002684 Genomic DNA. Translation: ANM59253.1. CP002684 Genomic DNA. Translation: ANM59254.1. CP002684 Genomic DNA. Translation: ANM59255.1. CP002684 Genomic DNA. Translation: ANM59256.1. CP002684 Genomic DNA. Translation: ANM59257.1. CP002684 Genomic DNA. Translation: ANM59259.1. BT003073 mRNA. Translation: AAO23638.1. AK227344 mRNA. Translation: BAE99355.1. AY084676 mRNA. Translation: AAM61238.1. DQ027047 mRNA. Translation: AAY44873.1.
PIRⁱ	C86309.
NCBI Reference Sequences More...RefSeqⁱ	NP_001077554.1. NM_001084085.1. NP_001319027.1. NM_001332287.1. NP_001321626.1. NM_001332292.1. NP_001321627.1. NM_001332293.1. NP_001321628.1. NM_001332288.1. NP_001321629.1. NM_001332290.1. NP_001321630.1. NM_001332291.1. NP_173172.1. NM_101590.4.
UniGeneⁱ	At.42947.

Genome annotation databases

EnsemblPlantsⁱ	AT1G17280.1; AT1G17280.1; AT1G17280. AT1G17280.2; AT1G17280.2; AT1G17280. AT1G17280.3; AT1G17280.3; AT1G17280. AT1G17280.5; AT1G17280.5; AT1G17280. AT1G17280.6; AT1G17280.6; AT1G17280. AT1G17280.7; AT1G17280.7; AT1G17280. AT1G17280.8; AT1G17280.8; AT1G17280. AT1G17280.9; AT1G17280.9; AT1G17280.
GeneIDⁱ	838300.
Grameneⁱ	AT1G17280.1; AT1G17280.1; AT1G17280. AT1G17280.2; AT1G17280.2; AT1G17280. AT1G17280.3; AT1G17280.3; AT1G17280. AT1G17280.5; AT1G17280.5; AT1G17280. AT1G17280.6; AT1G17280.6; AT1G17280. AT1G17280.7; AT1G17280.7; AT1G17280. AT1G17280.8; AT1G17280.8; AT1G17280. AT1G17280.9; AT1G17280.9; AT1G17280.
KEGGⁱ	ath:AT1G17280.

Cross-referencesⁱ

Web resourcesⁱ

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AC007651 Genomic DNA. Translation: AAD50006.1. CP002684 Genomic DNA. Translation: AEE29567.1. CP002684 Genomic DNA. Translation: AEE29568.1. CP002684 Genomic DNA. Translation: ANM59253.1. CP002684 Genomic DNA. Translation: ANM59254.1. CP002684 Genomic DNA. Translation: ANM59255.1. CP002684 Genomic DNA. Translation: ANM59256.1. CP002684 Genomic DNA. Translation: ANM59257.1. CP002684 Genomic DNA. Translation: ANM59259.1. BT003073 mRNA. Translation: AAO23638.1. AK227344 mRNA. Translation: BAE99355.1. AY084676 mRNA. Translation: AAM61238.1. DQ027047 mRNA. Translation: AAY44873.1.
PIRⁱ	C86309.
NCBI Reference Sequences More...RefSeqⁱ	NP_001077554.1. NM_001084085.1. NP_001319027.1. NM_001332287.1. NP_001321626.1. NM_001332292.1. NP_001321627.1. NM_001332293.1. NP_001321628.1. NM_001332288.1. NP_001321629.1. NM_001332290.1. NP_001321630.1. NM_001332291.1. NP_173172.1. NM_101590.4.
UniGeneⁱ	At.42947.

3D structure databases

ProteinModelPortalⁱ	Q9SHI7.
SMRⁱ	Q9SHI7.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	23540. 448 interactors.
IntActⁱ	Q9SHI7. 1 interactor.
STRINGⁱ	3702.AT1G17280.1.

Proteomic databases

PaxDbⁱ	Q9SHI7.

PaxDbⁱ

Q9SHI7.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT1G17280.1; AT1G17280.1; AT1G17280. AT1G17280.2; AT1G17280.2; AT1G17280. AT1G17280.3; AT1G17280.3; AT1G17280. AT1G17280.5; AT1G17280.5; AT1G17280. AT1G17280.6; AT1G17280.6; AT1G17280. AT1G17280.7; AT1G17280.7; AT1G17280. AT1G17280.8; AT1G17280.8; AT1G17280. AT1G17280.9; AT1G17280.9; AT1G17280.
GeneIDⁱ	838300.
Grameneⁱ	AT1G17280.1; AT1G17280.1; AT1G17280. AT1G17280.2; AT1G17280.2; AT1G17280. AT1G17280.3; AT1G17280.3; AT1G17280. AT1G17280.5; AT1G17280.5; AT1G17280. AT1G17280.6; AT1G17280.6; AT1G17280. AT1G17280.7; AT1G17280.7; AT1G17280. AT1G17280.8; AT1G17280.8; AT1G17280. AT1G17280.9; AT1G17280.9; AT1G17280.
KEGGⁱ	ath:AT1G17280.

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT1G17280.
TAIRⁱ	locus:2020392. AT1G17280.

Phylogenomic databases

eggNOGⁱ	KOG0894. Eukaryota. ENOG410Z0AN. LUCA.
HOGENOMⁱ	HOG000170329.
InParanoidⁱ	Q9SHI7.
KEGG Orthology (KO) More...KOⁱ	K04554.
OMAⁱ	FQPPSIY.
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360KZX.
PhylomeDBⁱ	Q9SHI7.

Enzyme and pathway databases

UniPathwayⁱ	UPA00143.

UniPathwayⁱ

UPA00143.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9SHI7.

PROⁱ

PR:Q9SHI7.

Gene expression databases

ExpressionAtlasⁱ	Q9SHI7. baseline.
Genevisibleⁱ	Q9SHI7. AT.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195. UBCc. 1 hit.
Gene3Dⁱ	3.10.110.10. 1 hit.
InterProⁱ	View protein in InterPro IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179. UQ_con. 1 hit.
SUPFAMⁱ	SSF54495. SSF54495. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBC34_ARATH
Accessionⁱ	Q9SHI7Primary (citable) accession number: Q9SHI7 Secondary accession number(s): Q4TYX7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
	Last sequence update:	May 1, 2000
	Last modified:	June 7, 2017
	This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names
PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9SHI7 (UBC34_ARATH)

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Ubiquitin-conjugating enzyme E2 34

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ