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Q9SHI1 (IF2C_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2, chloroplastic
Gene names
Ordered Locus Names:At1g17220
ORF Names:F20D23.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1026 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100_B

Subcellular location

Plastidchloroplast HAMAP-Rule MF_00100_B.

Sequence similarities

Belongs to the IF-2 family.

Sequence caution

The sequence AAD50011.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6363Chloroplast Potential
Chain64 – 1026963Translation initiation factor IF-2, chloroplastic HAMAP-Rule MF_00100_B
PRO_0000014477

Regions

Nucleotide binding508 – 5158GTP By similarity
Nucleotide binding558 – 5625GTP By similarity
Nucleotide binding612 – 6154GTP By similarity
Region502 – 654153G-domain HAMAP-Rule MF_00100_B

Experimental info

Sequence conflict7791R → G in AAN46761. Ref.3
Sequence conflict7791R → G in AAK32930. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9SHI1 [UniParc].

Last modified May 31, 2011. Version 2.
Checksum: 9D2E8620C2E7BB1D

FASTA1,026109,747
        10         20         30         40         50         60 
MPSMLVLVGT MPSLASLVSL GGACASVSGT SSSDASYALV KRVSLSRRSV KGTKKWLCRY 

        70         80         90        100        110        120 
SVSSSTTTTT ADFIADQNNN SVSIDSNSFR GSKDGDDSEV VLKQTPKPVL KPPVARVERG 

       130        140        150        160        170        180 
LGVNTAPWSK DLSNGGKFDG EEERNKVIES LGEVLDKAEK LEIPKPGNKE GGEAVKPSQP 

       190        200        210        220        230        240 
SANSSNSRNG SYANASDGGT RKTKTMKSVW RKGDAVAAVQ KVVKESPKIF NRGVQTEPRT 

       250        260        270        280        290        300 
REEGEVNAKA GTPLAPPQPP FRPQPPVRPQ PMLQGKPMVA PPVKKSPILK DLGMAAKPLV 

       310        320        330        340        350        360 
SEEVDSSVKS KERKPILVDK FASKKKGVDP AASQAVLAPT KPGKGPPSNK FRVEHRNKKN 

       370        380        390        400        410        420 
ASASPRRRIV AEDDGDDDAS ISRSGRKGRK WSKASRKAVR LQAAKDAAPV KAEILEVEEE 

       430        440        450        460        470        480 
GMSIEDLAYN LAIGEGDILG YLYSKGIRPD GVHTLDREMV KMICRDYDVE VLDADSVKVE 

       490        500        510        520        530        540 
EMAKKRQTFD EEDLDKLEDR PPVITIMGHV DHGKTTLLDY IRKSKVAASE AGGITQGIGA 

       550        560        570        580        590        600 
YKVSVPVDGK LQSCVFLDTP GHEAFGAMRA RGARVTDIAI IVVAADDGIR PQTNEAIAHA 

       610        620        630        640        650        660 
KAAAVPIVIA INKIDKEGAS PDRVMQELSS IGLMPEDWGG DVPMVQISAL KGENVDDLLE 

       670        680        690        700        710        720 
TVMLVAELQE LKANPHRNAK GIVIEAGLDK AKGPFATFIV QKGTLKRGDV VVCGEAFGKV 

       730        740        750        760        770        780 
RALFDHSGER VDEAGPSIPV QVIGLNNVPI AGDEFEIVSS LDVAREMAEA RAVSLRDERI 

       790        800        810        820        830        840 
SAKAGDGKVT LSSLASAVSA KKMSGLDLHQ LNIILKVDVQ GSIEAVRQAL QVLPQENVTL 

       850        860        870        880        890        900 
KFLLQATGDV SNSDVDLASA SEAIVFGFNV KASGSVKKAA ENKGVEIRLY RVIYELIDDV 

       910        920        930        940        950        960 
RNAMEGLLES VEEQIPIGSA EVRATFSSGS GRVAGCMVNE GKFVKDCGIR VVRKGKTVHV 

       970        980        990       1000       1010       1020 
GVLDSLKRVK ENVKEVSAGL ECGIGMDDYD DWIEGDIIEA FNAVQKRRTL EEASASMSAA 


IEEAGV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 231-1026.
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC007651 Genomic DNA. Translation: AAD50011.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE29560.1.
AF367343 mRNA. Translation: AAK32930.1.
BT001007 mRNA. Translation: AAN46761.1.
AK317746 mRNA. Translation: BAH20402.1.
PIRD86308.
RefSeqNP_173165.1. NM_101583.3.
UniGeneAt.18936.

3D structure databases

ProteinModelPortalQ9SHI1.
SMRQ9SHI1. Positions 420-1006.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9SHI1.
PRIDEQ9SHI1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G17220.1; AT1G17220.1; AT1G17220.
GeneID838293.
KEGGath:AT1G17220.

Organism-specific databases

TAIRAT1G17220.

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076906.
InParanoidQ9SHI1.
KOK02519.
OMALECGIGM.

Enzyme and pathway databases

BioCycARA:GQT-1416-MONOMER.

Gene expression databases

GenevestigatorQ9SHI1.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF04760. IF2_N. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2C_ARATH
AccessionPrimary (citable) accession number: Q9SHI1
Secondary accession number(s): B9DI35, Q9ASQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 31, 2011
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names