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Q9SHI1

- IF2C_ARATH

UniProt

Q9SHI1 - IF2C_ARATH

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Protein

Translation initiation factor IF-2, chloroplastic

Gene

At1g17220

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi508 – 5158GTPBy similarity
Nucleotide bindingi558 – 5625GTPBy similarity
Nucleotide bindingi612 – 6154GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. translation initiation factor activity Source: TAIR

GO - Biological processi

  1. translational initiation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1416-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-2, chloroplastic
Gene namesi
Ordered Locus Names:At1g17220
ORF Names:F20D23.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G17220.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6363ChloroplastSequence AnalysisAdd
BLAST
Chaini64 – 1026963Translation initiation factor IF-2, chloroplasticPRO_0000014477Add
BLAST

Proteomic databases

PaxDbiQ9SHI1.
PRIDEiQ9SHI1.

Expressioni

Gene expression databases

GenevestigatoriQ9SHI1.

Structurei

3D structure databases

ProteinModelPortaliQ9SHI1.
SMRiQ9SHI1. Positions 473-1005.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini499 – 672174tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni508 – 5158G1By similarity
Regioni533 – 5375G2By similarity
Regioni558 – 5614G3By similarity
Regioni612 – 6154G4By similarity
Regioni648 – 6503G5By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0532.
HOGENOMiHOG000076906.
InParanoidiQ9SHI1.
KOiK02519.
OMAiMKSVWRK.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERiPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF04760. IF2_N. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SHI1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSMLVLVGT MPSLASLVSL GGACASVSGT SSSDASYALV KRVSLSRRSV
60 70 80 90 100
KGTKKWLCRY SVSSSTTTTT ADFIADQNNN SVSIDSNSFR GSKDGDDSEV
110 120 130 140 150
VLKQTPKPVL KPPVARVERG LGVNTAPWSK DLSNGGKFDG EEERNKVIES
160 170 180 190 200
LGEVLDKAEK LEIPKPGNKE GGEAVKPSQP SANSSNSRNG SYANASDGGT
210 220 230 240 250
RKTKTMKSVW RKGDAVAAVQ KVVKESPKIF NRGVQTEPRT REEGEVNAKA
260 270 280 290 300
GTPLAPPQPP FRPQPPVRPQ PMLQGKPMVA PPVKKSPILK DLGMAAKPLV
310 320 330 340 350
SEEVDSSVKS KERKPILVDK FASKKKGVDP AASQAVLAPT KPGKGPPSNK
360 370 380 390 400
FRVEHRNKKN ASASPRRRIV AEDDGDDDAS ISRSGRKGRK WSKASRKAVR
410 420 430 440 450
LQAAKDAAPV KAEILEVEEE GMSIEDLAYN LAIGEGDILG YLYSKGIRPD
460 470 480 490 500
GVHTLDREMV KMICRDYDVE VLDADSVKVE EMAKKRQTFD EEDLDKLEDR
510 520 530 540 550
PPVITIMGHV DHGKTTLLDY IRKSKVAASE AGGITQGIGA YKVSVPVDGK
560 570 580 590 600
LQSCVFLDTP GHEAFGAMRA RGARVTDIAI IVVAADDGIR PQTNEAIAHA
610 620 630 640 650
KAAAVPIVIA INKIDKEGAS PDRVMQELSS IGLMPEDWGG DVPMVQISAL
660 670 680 690 700
KGENVDDLLE TVMLVAELQE LKANPHRNAK GIVIEAGLDK AKGPFATFIV
710 720 730 740 750
QKGTLKRGDV VVCGEAFGKV RALFDHSGER VDEAGPSIPV QVIGLNNVPI
760 770 780 790 800
AGDEFEIVSS LDVAREMAEA RAVSLRDERI SAKAGDGKVT LSSLASAVSA
810 820 830 840 850
KKMSGLDLHQ LNIILKVDVQ GSIEAVRQAL QVLPQENVTL KFLLQATGDV
860 870 880 890 900
SNSDVDLASA SEAIVFGFNV KASGSVKKAA ENKGVEIRLY RVIYELIDDV
910 920 930 940 950
RNAMEGLLES VEEQIPIGSA EVRATFSSGS GRVAGCMVNE GKFVKDCGIR
960 970 980 990 1000
VVRKGKTVHV GVLDSLKRVK ENVKEVSAGL ECGIGMDDYD DWIEGDIIEA
1010 1020
FNAVQKRRTL EEASASMSAA IEEAGV
Length:1,026
Mass (Da):109,747
Last modified:May 31, 2011 - v2
Checksum:i9D2E8620C2E7BB1D
GO

Sequence cautioni

The sequence AAD50011.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti779 – 7791R → G in AAN46761. (PubMed:14593172)Curated
Sequence conflicti779 – 7791R → G in AAK32930. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007651 Genomic DNA. Translation: AAD50011.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE29560.1.
AF367343 mRNA. Translation: AAK32930.1.
BT001007 mRNA. Translation: AAN46761.1.
AK317746 mRNA. Translation: BAH20402.1.
PIRiD86308.
RefSeqiNP_173165.1. NM_101583.3.
UniGeneiAt.18936.

Genome annotation databases

EnsemblPlantsiAT1G17220.1; AT1G17220.1; AT1G17220.
GeneIDi838293.
KEGGiath:AT1G17220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007651 Genomic DNA. Translation: AAD50011.1 . Different initiation.
CP002684 Genomic DNA. Translation: AEE29560.1 .
AF367343 mRNA. Translation: AAK32930.1 .
BT001007 mRNA. Translation: AAN46761.1 .
AK317746 mRNA. Translation: BAH20402.1 .
PIRi D86308.
RefSeqi NP_173165.1. NM_101583.3.
UniGenei At.18936.

3D structure databases

ProteinModelPortali Q9SHI1.
SMRi Q9SHI1. Positions 473-1005.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9SHI1.
PRIDEi Q9SHI1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G17220.1 ; AT1G17220.1 ; AT1G17220 .
GeneIDi 838293.
KEGGi ath:AT1G17220.

Organism-specific databases

TAIRi AT1G17220.

Phylogenomic databases

eggNOGi COG0532.
HOGENOMi HOG000076906.
InParanoidi Q9SHI1.
KOi K02519.
OMAi MKSVWRK.

Enzyme and pathway databases

BioCyci ARA:GQT-1416-MONOMER.

Gene expression databases

Genevestigatori Q9SHI1.

Family and domain databases

Gene3Di 3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00100_B. IF_2_B.
InterProi IPR000795. EF_GTP-bd_dom.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
PANTHERi PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF04760. IF2_N. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 231-1026.
    Strain: cv. Columbia.

Entry informationi

Entry nameiIF2C_ARATH
AccessioniPrimary (citable) accession number: Q9SHI1
Secondary accession number(s): B9DI35, Q9ASQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 31, 2011
Last modified: October 1, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3