Q9SHI1 (IF2C_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-2, chloroplastic

Gene names

Ordered Locus Names:	At1g17220
ORF Names:	F20D23.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1026 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the IF-2 family.
Sequence caution	The sequence AAD50011.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	chloroplast envelope Inferred from direct assay PubMed 12938931. Source: TAIR chloroplast stroma Inferred from direct assay PubMed 20061580. Source: TAIR
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from genetic interaction PubMed 17416734. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 63	63	Chloroplast Potential
	Chain	64 – 1026	963	Translation initiation factor IF-2, chloroplastic		PRO_0000014477
Regions
	Nucleotide binding	508 – 515	8	GTP By similarity
	Nucleotide binding	558 – 562	5	GTP By similarity
	Nucleotide binding	612 – 615	4	GTP By similarity
	Region	502 – 654	153	G-domain
Experimental info
	Sequence conflict	779	1	R → G in AAN46761. Ref.3
	Sequence conflict	779	1	R → G in AAK32930. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9SHI1 [UniParc]. Last modified May 31, 2011. Version 2. Checksum: 9D2E8620C2E7BB1D	FASTA	1,026	109,747
	10 20 30 40 50 60 MPSMLVLVGT MPSLASLVSL GGACASVSGT SSSDASYALV KRVSLSRRSV KGTKKWLCRY 70 80 90 100 110 120 SVSSSTTTTT ADFIADQNNN SVSIDSNSFR GSKDGDDSEV VLKQTPKPVL KPPVARVERG 130 140 150 160 170 180 LGVNTAPWSK DLSNGGKFDG EEERNKVIES LGEVLDKAEK LEIPKPGNKE GGEAVKPSQP 190 200 210 220 230 240 SANSSNSRNG SYANASDGGT RKTKTMKSVW RKGDAVAAVQ KVVKESPKIF NRGVQTEPRT 250 260 270 280 290 300 REEGEVNAKA GTPLAPPQPP FRPQPPVRPQ PMLQGKPMVA PPVKKSPILK DLGMAAKPLV 310 320 330 340 350 360 SEEVDSSVKS KERKPILVDK FASKKKGVDP AASQAVLAPT KPGKGPPSNK FRVEHRNKKN 370 380 390 400 410 420 ASASPRRRIV AEDDGDDDAS ISRSGRKGRK WSKASRKAVR LQAAKDAAPV KAEILEVEEE 430 440 450 460 470 480 GMSIEDLAYN LAIGEGDILG YLYSKGIRPD GVHTLDREMV KMICRDYDVE VLDADSVKVE 490 500 510 520 530 540 EMAKKRQTFD EEDLDKLEDR PPVITIMGHV DHGKTTLLDY IRKSKVAASE AGGITQGIGA 550 560 570 580 590 600 YKVSVPVDGK LQSCVFLDTP GHEAFGAMRA RGARVTDIAI IVVAADDGIR PQTNEAIAHA 610 620 630 640 650 660 KAAAVPIVIA INKIDKEGAS PDRVMQELSS IGLMPEDWGG DVPMVQISAL KGENVDDLLE 670 680 690 700 710 720 TVMLVAELQE LKANPHRNAK GIVIEAGLDK AKGPFATFIV QKGTLKRGDV VVCGEAFGKV 730 740 750 760 770 780 RALFDHSGER VDEAGPSIPV QVIGLNNVPI AGDEFEIVSS LDVAREMAEA RAVSLRDERI 790 800 810 820 830 840 SAKAGDGKVT LSSLASAVSA KKMSGLDLHQ LNIILKVDVQ GSIEAVRQAL QVLPQENVTL 850 860 870 880 890 900 KFLLQATGDV SNSDVDLASA SEAIVFGFNV KASGSVKKAA ENKGVEIRLY RVIYELIDDV 910 920 930 940 950 960 RNAMEGLLES VEEQIPIGSA EVRATFSSGS GRVAGCMVNE GKFVKDCGIR VVRKGKTVHV 970 980 990 1000 1010 1020 GVLDSLKRVK ENVKEVSAGL ECGIGMDDYD DWIEGDIIEA FNAVQKRRTL EEASASMSAA IEEAGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 231-1026. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007651 Genomic DNA. Translation: AAD50011.1. Different initiation. CP002684 Genomic DNA. Translation: AEE29560.1. AF367343 mRNA. Translation: AAK32930.1. BT001007 mRNA. Translation: AAN46761.1. AK317746 mRNA. Translation: BAH20402.1.
IPI	IPI00545652.
PIR	D86308.
RefSeq	NP_173165.1. NM_101583.3.
UniGene	At.18936.
3D structure databases
ProteinModelPortal	Q9SHI1.
SMR	Q9SHI1. Positions 458-1001.
ModBase	Search...
Proteomic databases
PaxDb	Q9SHI1.
PRIDE	Q9SHI1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G17220.1; AT1G17220.1; AT1G17220.
GeneID	838293.
KEGG	ath:AT1G17220.
Organism-specific databases
TAIR	At1g17220.
Phylogenomic databases
eggNOG	COG0532.
HOGENOM	HOG000076906.
InParanoid	Q9SHI1.
KO	K02519.
OMA	LECGIGM.
Gene expression databases
Genevestigator	Q9SHI1.
GermOnline	AT1G17220. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
InterPro	IPR000795. EF_GTP-bd_dom. IPR006847. IF2_N. IPR005225. Small_GTP-bd_dom. IPR000178. TF_IF2_bacterial-like. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. PF04760. IF2_N. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 2 hits.
TIGRFAMs	TIGR00487. IF-2. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2C_ARATH

Accession

Primary (citable) accession number: Q9SHI1
Secondary accession number(s): B9DI35, Q9ASQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 31, 2011
Last modified:	May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program