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Q9SHI1

- IF2C_ARATH

UniProt

Q9SHI1 - IF2C_ARATH

Protein

Translation initiation factor IF-2, chloroplastic

Gene

At1g17220

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (31 May 2011)
      Previous versions | rss
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    Functioni

    One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi508 – 5158GTPBy similarity
    Nucleotide bindingi558 – 5625GTPBy similarity
    Nucleotide bindingi612 – 6154GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. translation initiation factor activity Source: TAIR

    GO - Biological processi

    1. translational initiation Source: TAIR

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-1416-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Translation initiation factor IF-2, chloroplastic
    Gene namesi
    Ordered Locus Names:At1g17220
    ORF Names:F20D23.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G17220.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast envelope Source: TAIR
    3. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6363ChloroplastSequence AnalysisAdd
    BLAST
    Chaini64 – 1026963Translation initiation factor IF-2, chloroplasticPRO_0000014477Add
    BLAST

    Proteomic databases

    PaxDbiQ9SHI1.
    PRIDEiQ9SHI1.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9SHI1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SHI1.
    SMRiQ9SHI1. Positions 473-1005.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini499 – 672174tr-type GAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni508 – 5158G1By similarity
    Regioni533 – 5375G2By similarity
    Regioni558 – 5614G3By similarity
    Regioni612 – 6154G4By similarity
    Regioni648 – 6503G5By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0532.
    HOGENOMiHOG000076906.
    InParanoidiQ9SHI1.
    KOiK02519.
    OMAiMKSVWRK.

    Family and domain databases

    Gene3Di3.40.50.10050. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00100_B. IF_2_B.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR006847. IF2_N.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR000178. TF_IF2_bacterial-like.
    IPR015760. TIF_IF2.
    IPR023115. TIF_IF2_dom3.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PANTHERiPTHR23115:SF41. PTHR23115:SF41. 1 hit.
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF11987. IF-2. 1 hit.
    PF04760. IF2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 2 hits.
    SSF52156. SSF52156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00487. IF-2. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS51722. G_TR_2. 1 hit.
    PS01176. IF2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SHI1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSMLVLVGT MPSLASLVSL GGACASVSGT SSSDASYALV KRVSLSRRSV     50
    KGTKKWLCRY SVSSSTTTTT ADFIADQNNN SVSIDSNSFR GSKDGDDSEV 100
    VLKQTPKPVL KPPVARVERG LGVNTAPWSK DLSNGGKFDG EEERNKVIES 150
    LGEVLDKAEK LEIPKPGNKE GGEAVKPSQP SANSSNSRNG SYANASDGGT 200
    RKTKTMKSVW RKGDAVAAVQ KVVKESPKIF NRGVQTEPRT REEGEVNAKA 250
    GTPLAPPQPP FRPQPPVRPQ PMLQGKPMVA PPVKKSPILK DLGMAAKPLV 300
    SEEVDSSVKS KERKPILVDK FASKKKGVDP AASQAVLAPT KPGKGPPSNK 350
    FRVEHRNKKN ASASPRRRIV AEDDGDDDAS ISRSGRKGRK WSKASRKAVR 400
    LQAAKDAAPV KAEILEVEEE GMSIEDLAYN LAIGEGDILG YLYSKGIRPD 450
    GVHTLDREMV KMICRDYDVE VLDADSVKVE EMAKKRQTFD EEDLDKLEDR 500
    PPVITIMGHV DHGKTTLLDY IRKSKVAASE AGGITQGIGA YKVSVPVDGK 550
    LQSCVFLDTP GHEAFGAMRA RGARVTDIAI IVVAADDGIR PQTNEAIAHA 600
    KAAAVPIVIA INKIDKEGAS PDRVMQELSS IGLMPEDWGG DVPMVQISAL 650
    KGENVDDLLE TVMLVAELQE LKANPHRNAK GIVIEAGLDK AKGPFATFIV 700
    QKGTLKRGDV VVCGEAFGKV RALFDHSGER VDEAGPSIPV QVIGLNNVPI 750
    AGDEFEIVSS LDVAREMAEA RAVSLRDERI SAKAGDGKVT LSSLASAVSA 800
    KKMSGLDLHQ LNIILKVDVQ GSIEAVRQAL QVLPQENVTL KFLLQATGDV 850
    SNSDVDLASA SEAIVFGFNV KASGSVKKAA ENKGVEIRLY RVIYELIDDV 900
    RNAMEGLLES VEEQIPIGSA EVRATFSSGS GRVAGCMVNE GKFVKDCGIR 950
    VVRKGKTVHV GVLDSLKRVK ENVKEVSAGL ECGIGMDDYD DWIEGDIIEA 1000
    FNAVQKRRTL EEASASMSAA IEEAGV 1026
    Length:1,026
    Mass (Da):109,747
    Last modified:May 31, 2011 - v2
    Checksum:i9D2E8620C2E7BB1D
    GO

    Sequence cautioni

    The sequence AAD50011.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti779 – 7791R → G in AAN46761. (PubMed:14593172)Curated
    Sequence conflicti779 – 7791R → G in AAK32930. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007651 Genomic DNA. Translation: AAD50011.1. Different initiation.
    CP002684 Genomic DNA. Translation: AEE29560.1.
    AF367343 mRNA. Translation: AAK32930.1.
    BT001007 mRNA. Translation: AAN46761.1.
    AK317746 mRNA. Translation: BAH20402.1.
    PIRiD86308.
    RefSeqiNP_173165.1. NM_101583.3.
    UniGeneiAt.18936.

    Genome annotation databases

    EnsemblPlantsiAT1G17220.1; AT1G17220.1; AT1G17220.
    GeneIDi838293.
    KEGGiath:AT1G17220.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007651 Genomic DNA. Translation: AAD50011.1 . Different initiation.
    CP002684 Genomic DNA. Translation: AEE29560.1 .
    AF367343 mRNA. Translation: AAK32930.1 .
    BT001007 mRNA. Translation: AAN46761.1 .
    AK317746 mRNA. Translation: BAH20402.1 .
    PIRi D86308.
    RefSeqi NP_173165.1. NM_101583.3.
    UniGenei At.18936.

    3D structure databases

    ProteinModelPortali Q9SHI1.
    SMRi Q9SHI1. Positions 473-1005.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9SHI1.
    PRIDEi Q9SHI1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G17220.1 ; AT1G17220.1 ; AT1G17220 .
    GeneIDi 838293.
    KEGGi ath:AT1G17220.

    Organism-specific databases

    TAIRi AT1G17220.

    Phylogenomic databases

    eggNOGi COG0532.
    HOGENOMi HOG000076906.
    InParanoidi Q9SHI1.
    KOi K02519.
    OMAi MKSVWRK.

    Enzyme and pathway databases

    BioCyci ARA:GQT-1416-MONOMER.

    Gene expression databases

    Genevestigatori Q9SHI1.

    Family and domain databases

    Gene3Di 3.40.50.10050. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00100_B. IF_2_B.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR006847. IF2_N.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR000178. TF_IF2_bacterial-like.
    IPR015760. TIF_IF2.
    IPR023115. TIF_IF2_dom3.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    PANTHERi PTHR23115:SF41. PTHR23115:SF41. 1 hit.
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF11987. IF-2. 1 hit.
    PF04760. IF2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 2 hits.
    SSF52156. SSF52156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00487. IF-2. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51722. G_TR_2. 1 hit.
    PS01176. IF2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 231-1026.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiIF2C_ARATH
    AccessioniPrimary (citable) accession number: Q9SHI1
    Secondary accession number(s): B9DI35, Q9ASQ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: May 31, 2011
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3