Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione S-transferase U25

Gene

GSTU25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  1. glutathione transferase activity Source: TAIR

GO - Biological processi

  1. 2,4,6-trinitrotoluene catabolic process Source: TAIR
  2. response to toxic substance Source: UniProtKB-KW
  3. toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Detoxification

Enzyme and pathway databases

BioCyciARA:AT1G17180-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase U25 (EC:2.5.1.18)
Short name:
AtGSTU25
Alternative name(s):
GST class-tau member 25
Gene namesi
Name:GSTU25
Ordered Locus Names:At1g17180
ORF Names:F20D23.12A
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G17180.

Subcellular locationi

Cytoplasmcytosol Curated

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. cytosol Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 221220Glutathione S-transferase U25PRO_0000413570Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei149 – 1491PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SHH7.
PRIDEiQ9SHH7.

Expressioni

Gene expression databases

GenevestigatoriQ9SHH7.

Interactioni

Protein-protein interaction databases

BioGridi23529. 2 interactions.
IntActiQ9SHH7. 2 interactions.
STRINGi3702.AT1G17180.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SHH7.
SMRiQ9SHH7. Positions 1-219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8280GST N-terminalAdd
BLAST
Domaini88 – 208121GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 142Glutathione bindingBy similarity
Regioni39 – 402Glutathione bindingBy similarity
Regioni53 – 542Glutathione bindingBy similarity
Regioni66 – 672Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Tau family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125749.
InParanoidiQ9SHH7.
KOiK00799.
OMAiFVDVAFV.
PhylomeDBiQ9SHH7.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SHH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEVILLDF WPSMFGMRTR IALEEKNVKF DYREQDLWNK SPILLEMNPV
60 70 80 90 100
HKKIPVLIHN GNPVCESLIQ IEYIDEVWPS KTPLLPSDPY QRAQAKFWGD
110 120 130 140 150
FIDKKVYASA RLIWGAKGEE HEAGKKEFIE ILKTLESELG DKTYFGGETF
160 170 180 190 200
GYVDIALIGF YSWFEAYEKF GSFSIEAECP KLIAWGKRCV ERESVAKSLP
210 220
DSEKIIKFVP ELRKKLGIEI E
Length:221
Mass (Da):25,591
Last modified:May 1, 2000 - v1
Checksum:iBCEB3254B39364A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007651 Genomic DNA. Translation: AAD50015.1.
CP002684 Genomic DNA. Translation: AEE29555.1.
AK118907 mRNA. Translation: BAC43490.1.
BT005643 mRNA. Translation: AAO64063.1.
PIRiH86307.
RefSeqiNP_173161.1. NM_101579.3.
UniGeneiAt.41849.

Genome annotation databases

EnsemblPlantsiAT1G17180.1; AT1G17180.1; AT1G17180.
GeneIDi838289.
KEGGiath:AT1G17180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007651 Genomic DNA. Translation: AAD50015.1.
CP002684 Genomic DNA. Translation: AEE29555.1.
AK118907 mRNA. Translation: BAC43490.1.
BT005643 mRNA. Translation: AAO64063.1.
PIRiH86307.
RefSeqiNP_173161.1. NM_101579.3.
UniGeneiAt.41849.

3D structure databases

ProteinModelPortaliQ9SHH7.
SMRiQ9SHH7. Positions 1-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23529. 2 interactions.
IntActiQ9SHH7. 2 interactions.
STRINGi3702.AT1G17180.1-P.

Proteomic databases

PaxDbiQ9SHH7.
PRIDEiQ9SHH7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G17180.1; AT1G17180.1; AT1G17180.
GeneIDi838289.
KEGGiath:AT1G17180.

Organism-specific databases

TAIRiAT1G17180.

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125749.
InParanoidiQ9SHH7.
KOiK00799.
OMAiFVDVAFV.
PhylomeDBiQ9SHH7.

Enzyme and pathway databases

BioCyciARA:AT1G17180-MONOMER.

Gene expression databases

GenevestigatoriQ9SHH7.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
    Wagner U., Edwards R., Dixon D.P., Mauch F.
    Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGSTUP_ARATH
AccessioniPrimary (citable) accession number: Q9SHH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: May 1, 2000
Last modified: March 4, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.