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Protein

6-phosphogluconate dehydrogenase, decarboxylating 1, chloroplastic

Gene

At1g64190

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH.By similarity

Catalytic activityi

6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

Pathwayi: pentose phosphate pathway

This protein is involved in step 3 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic (At1g24280), Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic (APG1), Glucose-6-phosphate 1-dehydrogenase (At3g27300), Glucose-6-phosphate 1-dehydrogenase (AXX17_At3g29760), Glucose-6-phosphate 1-dehydrogenase (G6PD4), Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic (At5g13110), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g25530), Glucose-6-phosphate 1-dehydrogenase (At5g35790), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g12520), Glucose-6-phosphate 1-dehydrogenase (G6PD5), Glucose-6-phosphate 1-dehydrogenase (G6PD5), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2 (ACG12), Glucose-6-phosphate 1-dehydrogenase (At1g09420), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g38480), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g09330), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (ACG9), Glucose-6-phosphate 1-dehydrogenase (At5g40760), Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (At1g09420)
  2. Probable 6-phosphogluconolactonase 5, chloroplastic (EMB2024), Probable 6-phosphogluconolactonase 3 (At5g24420), Probable 6-phosphogluconolactonase 4 (At5g24410), Probable 6-phosphogluconolactonase 1 (At1g13700), Probable 6-phosphogluconolactonase 2 (At3g49360)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At5g39410), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At3g01580), 6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (At5g41670), 6-phosphogluconate dehydrogenase, decarboxylating 3 (At3g02360), 6-phosphogluconate dehydrogenase, decarboxylating 1, chloroplastic (At1g64190), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At1g57660)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei108NADPBy similarity1
Binding sitei108SubstrateBy similarity1
Active sitei188Proton acceptorBy similarity1
Active sitei195Proton donorBy similarity1
Binding sitei196SubstrateBy similarity1
Binding sitei266Substrate; via amide nitrogenBy similarity1
Binding sitei293SubstrateBy similarity1
Binding sitei458Substrate; shared with dimeric partnerBy similarity1
Binding sitei464Substrate; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 18NADPBy similarity6
Nucleotide bindingi36 – 38NADPBy similarity3
Nucleotide bindingi80 – 82NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • D-gluconate metabolic process Source: UniProtKB-KW
  • pentose-phosphate shunt, oxidative branch Source: TAIR
  • response to salt stress Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processGluconate utilization, Pentose shunt
LigandNADP

Enzyme and pathway databases

BioCyciARA:AT1G64190-MONOMER
ReactomeiR-ATH-71336 Pentose phosphate pathway (hexose monophosphate shunt)
UniPathwayiUPA00115; UER00410

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphogluconate dehydrogenase, decarboxylating 1, chloroplastic (EC:1.1.1.44)
Gene namesi
Ordered Locus Names:At1g64190
ORF Names:F22C12.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G64190
TAIRilocus:2024542 AT1G64190

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004210981 – 4876-phosphogluconate dehydrogenase, decarboxylating 1, chloroplasticAdd BLAST487

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9SH69
PRIDEiQ9SH69

PTM databases

iPTMnetiQ9SH69

Expressioni

Gene expression databases

ExpressionAtlasiQ9SH69 baseline and differential
GenevisibleiQ9SH69 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi27945, 3 interactors
IntActiQ9SH69, 1 interactor
STRINGi3702.AT1G64190.1

Structurei

3D structure databases

ProteinModelPortaliQ9SH69
SMRiQ9SH69
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 136Substrate bindingBy similarity3
Regioni191 – 192Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2653 Eukaryota
COG0362 LUCA
HOGENOMiHOG000255147
InParanoidiQ9SH69
KOiK00033
OMAiNSHYPDS
OrthoDBiEOG09360AB8
PhylomeDBiQ9SH69

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR006114 6PGDH_C
IPR006113 6PGDH_Gnd/GntZ
IPR006115 6PGDH_NADP-bd
IPR036291 NAD(P)-bd_dom_sf
IPR006183 Pgluconate_DH
PfamiView protein in Pfam
PF00393 6PGD, 1 hit
PF03446 NAD_binding_2, 1 hit
PIRSFiPIRSF000109 6PGD, 1 hit
PRINTSiPR00076 6PGDHDRGNASE
SMARTiView protein in SMART
SM01350 6PGD, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00873 gnd, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SH69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESAALSRIG LAGLAVMGQN LALNIAEKGF PISVYNRTTS KVDETLDRAA
60 70 80 90 100
VEGNLPVSGQ YSPRDFVLSI QRPRSLIILV KAGAPVDQTI DAFSEYMEPG
110 120 130 140 150
DCIIDGGNEW YQNTERRISE AEQKGLLYLG MGVSGGEEGA RNGPSLMPGG
160 170 180 190 200
SFQAYDNIKD ILEKVAAQVE DGPCVTYIGE GGSGNFVKMV HNGIEYGDMQ
210 220 230 240 250
LISEAYDVLK NVGGLSNEEL AEIFTEWNSG ELESFLVEIT SDIFRVKDEF
260 270 280 290 300
GDGELVDKIL DKTGMKGTGK WTVQQAAELS VAAPTIAASL DCRYLSGLKD
310 320 330 340 350
ERENAAKVLR EAGLKEEIGS ASSGIDKKRL VDDVRQALYA SKICSYAQGM
360 370 380 390 400
NLLRAKSLEK SWNLNFGELA RIWKGGCIIR AVFLDRIKKA YQRNPDLASL
410 420 430 440 450
VVDPEFAKEM VQRQAAWRRV VGLAVSAGIS TPGMCASLAY FDTYRRARLP
460 470 480
ANLVQAQRDL FGAHTYERTD RPGAYHTEWT KLARKNH
Length:487
Mass (Da):53,378
Last modified:May 1, 2000 - v1
Checksum:i2D5F2C25CF2827BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007764 Genomic DNA Translation: AAF24560.1
CP002684 Genomic DNA Translation: AEE34207.1
BT004568 mRNA Translation: AAO42814.1
AK227596 mRNA Translation: BAE99587.1
RefSeqiNP_176601.1, NM_105093.3
UniGeneiAt.43563
At.70356

Genome annotation databases

EnsemblPlantsiAT1G64190.1; AT1G64190.1; AT1G64190
GeneIDi842724
GrameneiAT1G64190.1; AT1G64190.1; AT1G64190
KEGGiath:AT1G64190

Similar proteinsi

Entry informationi

Entry namei6PGD1_ARATH
AccessioniPrimary (citable) accession number: Q9SH69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health