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Protein

Probable copper-transporting ATPase HMA5

Gene

HMA5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in copper import into the cell. May play a role in copper detoxification in roots.1 Publication

Catalytic activityi

ATP + H2O + Cu+(Side 1) = ADP + phosphate + Cu+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621CopperPROSITE-ProRule annotation
Metal bindingi65 – 651CopperPROSITE-ProRule annotation
Metal bindingi140 – 1401CopperPROSITE-ProRule annotation
Metal bindingi143 – 1431CopperPROSITE-ProRule annotation
Active sitei661 – 66114-aspartylphosphate intermediateBy similarity
Metal bindingi866 – 8661MagnesiumPROSITE-ProRule annotation
Metal bindingi870 – 8701MagnesiumPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • copper ion transmembrane transport Source: GOC
  • detoxification of copper ion Source: TAIR
  • response to copper ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Copper transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G63440-MONOMER.
ARA:MONOMER-14503.
MetaCyc:MONOMER-14503.
BRENDAi3.6.3.4. 399.
ReactomeiR-ATH-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable copper-transporting ATPase HMA5 (EC:3.6.3.54)
Alternative name(s):
Probable copper-transporting ATPase 3
Protein HEAVY METAL ATPASE 5
Gene namesi
Name:HMA5
Ordered Locus Names:At1g63440
ORF Names:F2K11.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G63440.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 299299CytoplasmicSequence analysisAdd
BLAST
Transmembranei300 – 32122HelicalSequence analysisAdd
BLAST
Topological domaini322 – 34019ExtracellularSequence analysisAdd
BLAST
Transmembranei341 – 36020HelicalSequence analysisAdd
BLAST
Topological domaini361 – 3677CytoplasmicSequence analysis
Transmembranei368 – 38821HelicalSequence analysisAdd
BLAST
Topological domaini389 – 40618ExtracellularSequence analysisAdd
BLAST
Transmembranei407 – 42721HelicalSequence analysisAdd
BLAST
Topological domaini428 – 561134CytoplasmicSequence analysisAdd
BLAST
Transmembranei562 – 58423HelicalSequence analysisAdd
BLAST
Topological domaini585 – 60521ExtracellularSequence analysisAdd
BLAST
Transmembranei606 – 62318HelicalSequence analysisAdd
BLAST
Topological domaini624 – 920297CytoplasmicSequence analysisAdd
BLAST
Transmembranei921 – 94020HelicalSequence analysisAdd
BLAST
Topological domaini941 – 95212ExtracellularSequence analysisAdd
BLAST
Transmembranei953 – 97119HelicalSequence analysisAdd
BLAST
Topological domaini972 – 99524CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants are hypersensitive to copper excess.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 995995Probable copper-transporting ATPase HMA5PRO_0000046404Add
BLAST

Proteomic databases

PaxDbiQ9SH30.
PRIDEiQ9SH30.

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Inductioni

By copper.1 Publication

Gene expression databases

ExpressionAtlasiQ9SH30. baseline and differential.
GenevisibleiQ9SH30. AT.

Interactioni

Subunit structurei

Interacts with ATX1.1 Publication

Protein-protein interaction databases

BioGridi27871. 2 interactions.
IntActiQ9SH30. 1 interaction.
STRINGi3702.AT1G63440.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SH30.
SMRiQ9SH30. Positions 56-979.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 11867HMA 1PROSITE-ProRule annotationAdd
BLAST
Domaini130 – 19667HMA 2PROSITE-ProRule annotationAdd
BLAST
Domaini205 – 27167HMA 3; degeneratePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 HMA domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250397.
InParanoidiQ9SH30.
KOiK17686.
OMAiSEENHIW.
PhylomeDBiQ9SH30.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006122. HMA_Cu_ion-bd.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 3 hits.
[Graphical view]
SUPFAMiSSF55008. SSF55008. 3 hits.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
TIGR00003. TIGR00003. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SH30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKLLSLTC IRKERFSERY PLVRKHLTRS RDGGGGSSSE TAAFEIDDPI
60 70 80 90 100
SRAVFQVLGM TCSACAGSVE KAIKRLPGIH DAVIDALNNR AQILFYPNSV
110 120 130 140 150
DVETIRETIE DAGFEASLIE NEANERSRQV CRIRINGMTC TSCSSTIERV
160 170 180 190 200
LQSVNGVQRA HVALAIEEAE IHYDPRLSSY DRLLEEIENA GFEAVLISTG
210 220 230 240 250
EDVSKIDLKI DGELTDESMK VIERSLEALP GVQSVEISHG TDKISVLYKP
260 270 280 290 300
DVTGPRNFIQ VIESTVFGHS GHIKATIFSE GGVGRESQKQ GEIKQYYKSF
310 320 330 340 350
LWSLVFTVPV FLTAMVFMYI PGIKDLLMFK VINMLTVGEI IRCVLATPVQ
360 370 380 390 400
FVIGWRFYTG SYKALRRGSA NMDVLIALGT NAAYFYSLYT VLRAATSPDF
410 420 430 440 450
KGVDFFETSA MLISFIILGK YLEVMAKGKT SQAIAKLMNL APDTAILLSL
460 470 480 490 500
DKEGNVTGEE EIDGRLIQKN DVIKIVPGAK VASDGYVIWG QSHVNESMIT
510 520 530 540 550
GEARPVAKRK GDTVIGGTLN ENGVLHVKVT RVGSESALAQ IVRLVESAQL
560 570 580 590 600
AKAPVQKLAD RISKFFVPLV IFLSFSTWLA WFLAGKLHWY PESWIPSSMD
610 620 630 640 650
SFELALQFGI SVMVIACPCA LGLATPTAVM VGTGVGASQG VLIKGGQALE
660 670 680 690 700
RAHKVNCIVF DKTGTLTMGK PVVVKTKLLK NMVLREFYEL VAATEVNSEH
710 720 730 740 750
PLAKAIVEYA KKFRDDEENP AWPEACDFVS ITGKGVKATV KGREIMVGNK
760 770 780 790 800
NLMNDHKVII PDDAEELLAD SEDMAQTGIL VSINSELIGV LSVSDPLKPS
810 820 830 840 850
AREAISILKS MNIKSIMVTG DNWGTANSIA REVGIDSVIA EAKPEQKAEK
860 870 880 890 900
VKELQAAGHV VAMVGDGIND SPALVAADVG MAIGAGTDIA IEAADIVLMK
910 920 930 940 950
SNLEDVITAI DLSRKTFSRI RLNYVWALGY NLMGIPIAAG VLFPGTRFRL
960 970 980 990
PPWIAGAAMA ASSVSVVCCS LLLKNYKRPK KLDHLEIREI QVERV
Length:995
Mass (Da):108,336
Last modified:January 11, 2001 - v2
Checksum:iC9289E6FDE7D727B
GO

Sequence cautioni

The sequence AAF19707.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008047 Genomic DNA. Translation: AAF19707.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34100.1.
PIRiB96660.
RefSeqiNP_176533.1. NM_105023.1.
UniGeneiAt.36129.

Genome annotation databases

EnsemblPlantsiAT1G63440.1; AT1G63440.1; AT1G63440.
GeneIDi842650.
GrameneiAT1G63440.1; AT1G63440.1; AT1G63440.
KEGGiath:AT1G63440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008047 Genomic DNA. Translation: AAF19707.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34100.1.
PIRiB96660.
RefSeqiNP_176533.1. NM_105023.1.
UniGeneiAt.36129.

3D structure databases

ProteinModelPortaliQ9SH30.
SMRiQ9SH30. Positions 56-979.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27871. 2 interactions.
IntActiQ9SH30. 1 interaction.
STRINGi3702.AT1G63440.1.

Proteomic databases

PaxDbiQ9SH30.
PRIDEiQ9SH30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G63440.1; AT1G63440.1; AT1G63440.
GeneIDi842650.
GrameneiAT1G63440.1; AT1G63440.1; AT1G63440.
KEGGiath:AT1G63440.

Organism-specific databases

TAIRiAT1G63440.

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250397.
InParanoidiQ9SH30.
KOiK17686.
OMAiSEENHIW.
PhylomeDBiQ9SH30.

Enzyme and pathway databases

BioCyciARA:AT1G63440-MONOMER.
ARA:MONOMER-14503.
MetaCyc:MONOMER-14503.
BRENDAi3.6.3.4. 399.
ReactomeiR-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ9SH30.

Gene expression databases

ExpressionAtlasiQ9SH30. baseline and differential.
GenevisibleiQ9SH30. AT.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006122. HMA_Cu_ion-bd.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 3 hits.
[Graphical view]
SUPFAMiSSF55008. SSF55008. 3 hits.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
TIGR00003. TIGR00003. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "The Arabidopsis heavy metal P-type ATPase HMA5 interacts with metallochaperones and functions in copper detoxification of roots."
    Andres-Colas N., Sancenon V., Rodriguez-Navarro S., Mayo S., Thiele D.J., Ecker J.R., Puig S., Penarrubia L.
    Plant J. 45:225-236(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATX1, TISSUE SPECIFICITY, INDUCTION BY COPPER, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiHMA5_ARATH
AccessioniPrimary (citable) accession number: Q9SH30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: April 13, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.