Putative copper-transporting ATPase 3 - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q9SH30 (AHM7_ARATH)

Last modified June 16, 2009. Version 75. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Putative copper-transporting ATPase 3
EC=3.6.3.4

Gene names

Name:	HMA5
Ordered Locus Names:	At1g63440
ORF Names:	F2K11.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	995 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Involved in copper import into the cell.
Catalytic activity	ATP + H₂O + Cu²⁺(In) = ADP + phosphate + Cu²⁺(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IB subfamily. Contains 3 HMA domains.
Sequence caution	The sequence AAF19707.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords
Biological process	Copper transport Ion transport Transport
Cellular component	Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Copper Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro copper ion transport Inferred from electronic annotation. Source: UniProtKB-KW detoxification of copper ion Inferred from mutant phenotype. Source: TAIR
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW copper-exporting ATPase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 995

995

Putative copper-transporting ATPase 3

PRO_0000046404

Regions

Topological domain

1 – 299

299

Cytoplasmic Potential

Transmembrane

300 – 321

Potential

Topological domain

322 – 340

Extracellular Potential

Transmembrane

341 – 360

Potential

Topological domain

361 – 367

Cytoplasmic Potential

Transmembrane

368 – 388

Potential

Topological domain

389 – 406

Extracellular Potential

Transmembrane

407 – 427

Potential

Topological domain

428 – 561

134

Cytoplasmic Potential

Transmembrane

562 – 584

Potential

Topological domain

585 – 605

Extracellular Potential

Transmembrane

606 – 623

Potential

Topological domain

624 – 920

297

Cytoplasmic Potential

Transmembrane

921 – 940

Potential

Topological domain

941 – 952

Extracellular Potential

Transmembrane

953 – 971

Potential

Topological domain

972 – 995

Cytoplasmic Potential

Domain

52 – 118

HMA 1

Domain

130 – 196

HMA 2

Domain

205 – 271

HMA 3; degenerate

Sites

Active site

661

4-aspartylphosphate intermediate By similarity

Metal binding

Copper Potential

Metal binding

Copper Potential

Metal binding

140

Copper Potential

Metal binding

143

Copper Potential

Metal binding

866

Magnesium By similarity

Metal binding

870

Magnesium By similarity

Amino acid modifications

Modified residue

601

Phosphoserine By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9SH30-1 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: C9289E6FDE7D727B
Show »

FASTA

995

108,336

        10         20         30         40         50         60 
MATKLLSLTC IRKERFSERY PLVRKHLTRS RDGGGGSSSE TAAFEIDDPI SRAVFQVLGM 

        70         80         90        100        110        120 
TCSACAGSVE KAIKRLPGIH DAVIDALNNR AQILFYPNSV DVETIRETIE DAGFEASLIE 

       130        140        150        160        170        180 
NEANERSRQV CRIRINGMTC TSCSSTIERV LQSVNGVQRA HVALAIEEAE IHYDPRLSSY 

       190        200        210        220        230        240 
DRLLEEIENA GFEAVLISTG EDVSKIDLKI DGELTDESMK VIERSLEALP GVQSVEISHG 

       250        260        270        280        290        300 
TDKISVLYKP DVTGPRNFIQ VIESTVFGHS GHIKATIFSE GGVGRESQKQ GEIKQYYKSF 

       310        320        330        340        350        360 
LWSLVFTVPV FLTAMVFMYI PGIKDLLMFK VINMLTVGEI IRCVLATPVQ FVIGWRFYTG 

       370        380        390        400        410        420 
SYKALRRGSA NMDVLIALGT NAAYFYSLYT VLRAATSPDF KGVDFFETSA MLISFIILGK 

       430        440        450        460        470        480 
YLEVMAKGKT SQAIAKLMNL APDTAILLSL DKEGNVTGEE EIDGRLIQKN DVIKIVPGAK 

       490        500        510        520        530        540 
VASDGYVIWG QSHVNESMIT GEARPVAKRK GDTVIGGTLN ENGVLHVKVT RVGSESALAQ 

       550        560        570        580        590        600 
IVRLVESAQL AKAPVQKLAD RISKFFVPLV IFLSFSTWLA WFLAGKLHWY PESWIPSSMD 

       610        620        630        640        650        660 
SFELALQFGI SVMVIACPCA LGLATPTAVM VGTGVGASQG VLIKGGQALE RAHKVNCIVF 

       670        680        690        700        710        720 
DKTGTLTMGK PVVVKTKLLK NMVLREFYEL VAATEVNSEH PLAKAIVEYA KKFRDDEENP 

       730        740        750        760        770        780 
AWPEACDFVS ITGKGVKATV KGREIMVGNK NLMNDHKVII PDDAEELLAD SEDMAQTGIL 

       790        800        810        820        830        840 
VSINSELIGV LSVSDPLKPS AREAISILKS MNIKSIMVTG DNWGTANSIA REVGIDSVIA 

       850        860        870        880        890        900 
EAKPEQKAEK VKELQAAGHV VAMVGDGIND SPALVAADVG MAIGAGTDIA IEAADIVLMK 

       910        920        930        940        950        960 
SNLEDVITAI DLSRKTFSRI RLNYVWALGY NLMGIPIAAG VLFPGTRFRL PPWIAGAAMA 

       970        980        990 
ASSVSVVCCS LLLKNYKRPK KLDHLEIREI QVERV

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References

[1]

"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.

Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.

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Cross-references

Sequence databases
	AC008047 Genomic DNA. Translation: AAF19707.1. Sequence problems.
IPI	IPI00548991.
PIR	B96660.
RefSeq	NP_176533.1.
UniGene	At.36129
3D structure databases
HSSP	HSSP built from PDB template 1AW0 based on UniProtKB Q04656.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SH30. 1 interaction.
Proteomic databases
PRIDE	Q9SH30.
Genome annotation databases
GeneID	842650.
GenomeReviews	Gene locus AT1G63440 in contig CT485782_GR.
KEGG	ath:AT1G63440.
NMPDR	fig\|3702.1.peg.5748.
Organism-specific databases
TAIR	At1g63440.
Phylogenomic databases
OMA	Q9SH30. SRVTPGA.
Enzyme and pathway databases
BRENDA	3.6.3.4. 302.
Gene expression databases
ArrayExpress	Q9SH30.
GermOnline	AT1G63440. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001877. ATPase1_Cu-transp. IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR006403. ATPase_P-typ_cat/Cu-transptr. IPR006416. ATPase_P-typ_heavy-metal. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_phosphor_site. IPR005834. Dehalogen-like_hydro. IPR017969. Heavy-metal-associated_CS. IPR006121. HeavyMe_transpt. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00403. HMA. 3 hits. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00942. CUATPASEI.
TIGRFAMs	TIGR01511. ATPase-IB1_Cu. 1 hit. TIGR01525. ATPase-IB_hvy. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. PS01047. HMA_1. 1 hit. PS50846. HMA_2. 3 hits. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

AHM7_ARATH

Accession

Primary (citable) accession number: Q9SH30

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents