Methionine gamma-lyase - Arabidopsis thaliana (Mouse-ear cress)

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Q9SGU9 (MGL_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 74. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Methionine gamma-lyase
Short name=AtMGL
EC=4.4.1.11

Alternative name(s):
L-methioninase

Gene names

Name:	MGL
Ordered Locus Names:	At1g64660
ORF Names:	F1N19.23

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the degradation of L-methionine to alpha-ketobutyrate, methanethiol and ammonia. Exhibits a high activity toward L-methionine, L-ethionine, L-homocysteine and seleno-L-methionine, but not L-cysteine. Involved in an alternative cysteine biosynthesis pathway to the reverse trans-sulfuration pathway (methionine->homocysteine->cystathionine->cysteine) in which methanethiol is an intermediate. Mediates also an alternative isoleucine biosynthesis pathway in which 2-ketobutyrate is an intermediate. Ref.6 Ref.7
Catalytic activity	L-methionine + H₂O = methanethiol + NH₃ + 2-oxobutanoate. Ref.5 Ref.6
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homotetramer. Ref.6
Subcellular location	Cytoplasm Ref.5.
Tissue specificity	Expressed in roots, stems, siliques, leaves, flowers and seeds after imbibition (at protein level). Transcripts accumulate in dry mature seeds, but at protein level, only present upon imbibition. Ref.5 Ref.6
Induction	By sulfate deprivation and high methionine levels. Up-regulated by drought. Ref.5 Ref.6 Ref.7
Disruption phenotype	Increased leaves, flowers and seeds methionine content, and leaf and root S-methylmethionine content under conditions of sulfate starvation. Reduced MGL-mediated isoleucine biosynthesis from methionine. Ref.6 Ref.7
Sequence similarities	Belongs to the trans-sulfuration enzymes family.
Biophysicochemical properties	Absorption: Abs(max)=422 nm Ref.5 Ref.6 Kinetic parameters: kcat is 9.5 sec(-1) with L-methionine as substrate, 8.9 sec(-1) with L-ethionine as substrate, 36.2 sec(-1) with L-homocysteine as substrate and 11.6 sec(-1) with seleno-L-methionine as substrate (Ref.6, at pH 8 and 30 degrees Celsius). K_M=10 mM for L-methionine (Ref.5, at pH 7.2 and 30 degrees Celsius) K_M=72 mM for L-methionine (Ref.6, at pH 8 and 30 degrees Celsius) K_M=14 mM for L-ethionine (Ref.6, at pH 8 and 30 degrees Celsius) K_M=92 mM for L-homocysteine (Ref.6, at pH 8 and 30 degrees Celsius) K_M=40 mM for seleno-L-methionine (Ref.6, at pH 8 and 30 degrees Celsius) V_max=194 nmol/min/mg enzyme with L-methionine as substrate (Ref.6, at pH 8 and 30 degrees Celsius) V_max=182 nmol/min/mg enzyme with L-ethionine as substrate (Ref.6, at pH 8 and 30 degrees Celsius) V_max=743 nmol/min/mg enzyme with L-homocysteine as substrate (Ref.6, at pH 8 and 30 degrees Celsius) V_max=238 nmol/min/mg enzyme with seleno-L-methionine as substrate (Ref.6, at pH 8 and 30 degrees Celsius)

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to sulfate starvation Inferred from expression pattern Ref.6. Source: UniProtKB cellular response to water deprivation Inferred from expression pattern Ref.7. Source: UniProtKB methionine catabolic process via 2-oxobutanoate Traceable author statement Ref.5. Source: TAIR protein homotetramerization Inferred from direct assay Ref.6. Source: UniProtKB
Cellular_component	cytosol Inferred from direct assay Ref.5. Source: TAIR
Molecular_function	methionine gamma-lyase activity Inferred from direct assay Ref.6. Source: UniProtKB pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 441

441

Methionine gamma-lyase

PRO_0000420159

Amino acid modifications

Modified residue

248

N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9SGU9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 8CF2025201571FA1
Show »

FASTA

441

47,821

        10         20         30         40         50         60 
MAHFLETQEP LVFSGKKRND RDDEDGDALV AKKSALAVCD ADPAAAIANI RHEFGEHGGV 

        70         80         90        100        110        120 
NMSIEASATF TVMEPDTMRR MFTGELGPDN DFYVYSRHFN PTVLNLSRQM AALEGTQAAY 

       130        140        150        160        170        180 
CTSSGMSAIS SVMLQLCSSG GHVVAASTLY GGTHALLSHF LPRTCNITTS FVDITDHGAV 

       190        200        210        220        230        240 
ANAIVEGRTQ VLYFESVANP TLTVADIPEL SRMAHEKGVT VVVDNTFAPM VLSPAKLGAD 

       250        260        270        280        290        300 
VVVHSISKFI SGGADIIAGA VCGSENLVKE MMDLRGGSLM LLGPTMNAKV AFELSERIPH 

       310        320        330        340        350        360 
LGLRMREHSH RAQVYAERMR DLGMKVIYPG LETHPQHKLF KGMVNRDYGY GGLLSIDMET 

       370        380        390        400        410        420 
EEKANKLMAY LQNATQFGFM AVSLGYYETL MSCSGSSTSS ELDPSQKEAA GISPGLVRMS 

       430        440 
VGYVGTLEQK WTQFEKAFLR M

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Methionine catabolism in Arabidopsis cells is initiated by a gamma-cleavage process and leads to S-methylcysteine and isoleucine syntheses." Rebeille F., Jabrin S., Bligny R., Loizeau K., Gambonnet B., Van Wilder V., Douce R., Ravanel S. Proc. Natl. Acad. Sci. U.S.A. 103:15687-15692(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, INDUCTION BY HIGH MET LEVELS, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
[6]	"Functional characterization of a methionine gamma-lyase in Arabidopsis and its implication in an alternative to the reverse trans-sulfuration pathway." Goyer A., Collakova E., Shachar-Hill Y., Hanson A.D. Plant Cell Physiol. 48:232-242(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, INDUCTION BY SULFATE DEPRIVATION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Columbia.
[7]	"Arabidopsis methionine gamma-lyase is regulated according to isoleucine biosynthesis needs but plays a subordinate role to threonine deaminase." Joshi V., Jander G. Plant Physiol. 151:367-378(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY DROUGHT. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC009519 Genomic DNA. Translation: AAF19680.1. CP002684 Genomic DNA. Translation: AEE34271.1. AF428413 mRNA. Translation: AAL16181.1. AY054546 mRNA. Translation: AAK96737.1. BT006588 mRNA. Translation: AAP31932.1. AK226375 mRNA. Translation: BAE98522.1.
IPI	IPI00518615.
PIR	G96669.
RefSeq	NP_176647.1. NM_105141.2.
UniGene	At.16566. At.35974.
3D structure databases
HSSP	HSSP built from PDB template 1GC0 based on UniProtKB P13254.
ProteinModelPortal	Q9SGU9.
SMR	Q9SGU9. Positions 43-422.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G64660.1-P.
Proteomic databases
PaxDb	Q9SGU9.
PRIDE	Q9SGU9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G64660.1; AT1G64660.1; AT1G64660.
GeneID	842774.
KEGG	ath:AT1G64660.
Organism-specific databases
TAIR	At1g64660.
Phylogenomic databases
eggNOG	COG0626.
HOGENOM	HOG000246415.
InParanoid	Q9SGU9.
KO	K01761.
OMA	FIDATRM.
PhylomeDB	Q9SGU9.
ProtClustDB	PLN02242.
Enzyme and pathway databases
BioCyc	MetaCyc:AT1G64660-MONOMER.
Gene expression databases
Genevestigator	Q9SGU9.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11808. PTHR11808. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGL_ARATH

Accession

Primary (citable) accession number: Q9SGU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2012
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents