Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9SGH2

- MBD9_ARATH

UniProt

Q9SGH2 - MBD9_ARATH

Protein

Methyl-CpG-binding domain-containing protein 9

Gene

MBD9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Probable transcriptional regulator that acts as a histone acetyltransferase. Mediates the acetylation of histone H3 and H4 of target loci (e.g. FLC). Involved in an auxin-independent regulation of shoot branching and flowering time.2 Publications

    Catalytic activityi

    Acetyl-CoA + [histone] = CoA + acetyl-[histone].

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri83 – 13351PHD-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri86 – 13146RING-type 1; degenerateAdd
    BLAST
    Zinc fingeri1287 – 133751PHD-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1290 – 133546RING-type 2; degenerateAdd
    BLAST

    GO - Molecular functioni

    1. histone acetyltransferase activity Source: UniProtKB
    2. histone binding Source: UniProtKB
    3. methyl-CpG binding Source: TAIR
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. histone acetylation Source: UniProtKB
    2. histone H3 acetylation Source: UniProtKB
    3. histone H4 acetylation Source: UniProtKB
    4. photoperiodism, flowering Source: TAIR
    5. regulation of transcription, DNA-templated Source: TAIR
    6. secondary shoot formation Source: TAIR
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methyl-CpG-binding domain-containing protein 9 (EC:2.3.1.48)
    Short name:
    AtMBD9
    Short name:
    MBD09
    Alternative name(s):
    Histone acetyl transferase MBD9
    Methyl-CpG-binding protein MBD9
    Gene namesi
    Name:MBD9
    Ordered Locus Names:At3g01460
    ORF Names:F4P13.1, T13O15.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G01460.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB
    2. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Over-methylated genomic DNA. Increased shoot branching and reduced transcription of FLC leading to early flowering, associated with a decrease in the acetylation level in histone H3 and H4 of FLC chromatin.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21762176Methyl-CpG-binding domain-containing protein 9PRO_0000405285Add
    BLAST

    Proteomic databases

    PaxDbiQ9SGH2.
    PRIDEiQ9SGH2.

    Expressioni

    Tissue specificityi

    Expressed in leaves, buds, flowers and stems.2 Publications

    Gene expression databases

    GenevestigatoriQ9SGH2.

    Interactioni

    Subunit structurei

    Interacts with histone H4.

    Protein-protein interaction databases

    STRINGi3702.AT3G01460.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SGH2.
    SMRiQ9SGH2. Positions 82-133, 267-308, 1180-1240, 1290-1334.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini258 – 32770MBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini403 – 45654FYR N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini550 – 698149FYR C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Repeati1098 – 113740PumilioAdd
    BLAST
    Domaini1157 – 122872BromoAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili491 – 51121Sequence AnalysisAdd
    BLAST
    Coiled coili1251 – 127323Sequence AnalysisAdd
    BLAST
    Coiled coili1410 – 143728Sequence AnalysisAdd
    BLAST
    Coiled coili1588 – 162841Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi914 – 9218Nuclear localization signalBy similarity
    Motifi1124 – 11318Nuclear localization signalBy similarity
    Motifi1256 – 12638Nuclear localization signalBy similarity
    Motifi1337 – 13448Nuclear localization signalBy similarity
    Motifi1761 – 17688Nuclear localization signalBy similarity

    Domaini

    The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.By similarity

    Sequence similaritiesi

    Contains 1 bromo domain.Curated
    Contains 1 FYR C-terminal domain.PROSITE-ProRule annotation
    Contains 1 FYR N-terminal domain.PROSITE-ProRule annotation
    Contains 1 MBD (methyl-CpG-binding) domain.PROSITE-ProRule annotation
    Contains 2 PHD-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 pumilio repeat.Curated
    Contains 2 RING-type zinc fingers.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri83 – 13351PHD-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri86 – 13146RING-type 1; degenerateAdd
    BLAST
    Zinc fingeri1287 – 133751PHD-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1290 – 133546RING-type 2; degenerateAdd
    BLAST

    Keywords - Domaini

    Bromodomain, Coiled coil, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG79337.
    HOGENOMiHOG000153462.
    InParanoidiQ9SGH2.
    OMAiFEEICSK.
    PhylomeDBiQ9SGH2.

    Family and domain databases

    Gene3Di3.30.40.10. 2 hits.
    InterProiIPR016177. DNA-bd_dom.
    IPR003889. FYrich_C.
    IPR003888. FYrich_N.
    IPR001739. Methyl_CpG_DNA-bd.
    IPR028942. WHIM1_dom.
    IPR019786. Zinc_finger_PHD-type_CS.
    IPR011011. Znf_FYVE_PHD.
    IPR001965. Znf_PHD.
    IPR019787. Znf_PHD-finger.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF05965. FYRC. 1 hit.
    PF05964. FYRN. 1 hit.
    PF01429. MBD. 1 hit.
    PF00628. PHD. 2 hits.
    PF15612. WHIM1. 1 hit.
    [Graphical view]
    SMARTiSM00249. PHD. 2 hits.
    SM00184. RING. 2 hits.
    [Graphical view]
    SUPFAMiSSF54171. SSF54171. 1 hit.
    SSF57903. SSF57903. 2 hits.
    PROSITEiPS51543. FYRC. 1 hit.
    PS51542. FYRN. 1 hit.
    PS50982. MBD. 1 hit.
    PS01359. ZF_PHD_1. 2 hits.
    PS50016. ZF_PHD_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9SGH2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPTDSTNEQ LGDTKTAAVK EESRSFLGID LNEIPTGATL GGGCTAGQDD     50
    DGEYEPVEVV RSIHDNPDPA PGAPAEVPEP DRDASCGACG RPESIELVVV 100
    CDACERGFHM SCVNDGVEAA PSADWMCSDC RTGGERSKLW PLGVKSKLIL 150
    DMNASPPSDA EGYGAEETSD SRKHMLASSS CIGNSFDYAM MHSSFSSLGR 200
    GHASLEASGL MSRNTKMSMD ALGSHNLGFG FPLNLNNSSL PMRFPSLDPS 250
    ELFLQNLRHF ISERHGVLED GWRVEFRQPL NGYQLCAVYC APNGKTFSSI 300
    QEVACYLGLA INGNYSCMDA EIRNENSLLQ ERLHTPKRRK TSRWPNNGFP 350
    EQKGSSVSAQ LRRFPFNGQT MSPFAVKSGT HFQAGGSLSS GNNGCGCEEA 400
    KNGCPMQFED FFVLSLGRID IRQSYHNVNV IYPIGYKSCW HDKITGSLFT 450
    CEVSDGNSGP IFKVTRSPCS KSFIPAGSTV FSCPKIDEMV EQNSDKLSNR 500
    RDSTQERDDD ASVEILLSEH CPPLGDDILS CLREKSFSKT VNSLRSEVDS 550
    SRVDFDKNLS YDQDHGVEIG DIVVEEDSLS DAWKKVSQKL VDACSIVLKQ 600
    KGTLNFLCKH VDRETSEINW DTMNEKDNVI LSLSKFCCSL APCSVTCGEK 650
    DKSEFAAVVD ALSRWLDQNR FGLDADFVQE MIEHMPGAES CTNYRTLKSR 700
    SSSSVPITVA EGALVVKPKG GENVKDEVFG EISRKAKKPK LNGGHGVRNL 750
    HPPPGRPMCL RLPPGLVGDF LQVSEVFWRF HEILGFEEAF SPENLEQELI 800
    NPVFDGLFLD KPGKDDKRSE INFTDKDSTA TKLFSLFDES RQPFPAKNTS 850
    ASELKEKKAG DSSDFKISDS SRGSCVGALL TRAHISLLQV LICELQSKVA 900
    AFVDPNFDSG ESRSRRGRKK DDSTLSAKRN KLHMLPVNEF TWPELARRYI 950
    LSLLSMDGNL ESAEIAARES GKVFRCLQGD GGLLCGSLTG VAGMEADSML 1000
    LAEAIKKISG SLTSENDVLS VEDDDSDGLD ATETNTCSGD IPEWAQVLEP 1050
    VKKLPTNVGT RIRKCVYEAL ERNPPEWAKK ILEHSISKEI YKGNASGPTK 1100
    KAVLSLLADI RGGDLVQRSI KGTKKRTYIS VSDVIMKKCR AVLRGVAAAD 1150
    EDKVLCTLLG RKLLNSSDND DDGLLGSPAM VSRPLDFRTI DLRLAAGAYD 1200
    GSTEAFLEDV LELWSSIRVM YADQPDCVDL VATLSEKFKS LYEAEVVPLV 1250
    QKLKDYRKLE CLSAEMKKEI KDIVVSVNKL PKAPWDEGVC KVCGVDKDDD 1300
    SVLLCDTCDA EYHTYCLNPP LIRIPDGNWY CPSCVIAKRM AQEALESYKL 1350
    VRRRKGRKYQ GELTRASMEL TAHLADVMEE KDYWEFSAEE RILLLKLLCD 1400
    ELLSSSLVHQ HLEQCAEAII EMQQKLRSLS SEWKNAKMRQ EFLTAKLAKV 1450
    EPSILKEVGE PHNSSYFADQ MGCDPQPQEG VGDGVTRDDE TSSTAYLNKN 1500
    QGKSPLETDT QPGESHVNFG ESKISSPETI SSPGRHELPI ADTSPLVTDN 1550
    LPEKDTSETL LKSVGRNHET HSPNSNAVEL PTAHDASSQA SQELQACQQD 1600
    LSATSNEIQN LQQSIRSIES QLLKQSIRRD FLGTDASGRL YWGCCFPDEN 1650
    PRILVDGSIS LQKPVQADLI GSKVPSPFLH TVDHGRLRLS PWTYYETETE 1700
    ISELVQWLHD DDLKERDLRE SILWWKRLRY GDVQKEKKQA QNLSAPVFAT 1750
    GLETKAAMSM EKRYGPCIKL EMETLKKRGK KTKVAEREKL CRCECLESIL 1800
    PSMIHCLICH KTFASDDEFE DHTESKCIPY SLATEEGKDI SDSSKAKESL 1850
    KSDYLNVKSS AGKDVAEISN VSELDSGLIR YQEEESISPY HFEEICSKFV 1900
    TKDCNRDLVK EIGLISSNGI PTFLPSSSTH LNDSVLISAK SNKPDGGDSG 1950
    DQVIFAGPET NVEGLNSESN MSFDRSVTDS HGGPLDKPSG LGFGFSEQKN 2000
    KKSSGSGLKS CCVVPQAALK RVTGKALPGF RFLKTNLLDM DVALPEEALR 2050
    PSKSHPNRRR AWRVFVKSSQ SIYELVQATI VVEDMIKTEY LKNEWWYWSS 2100
    LSAAAKISTL SALSVRIFSL DAAIIYDKPI TPSNPIDETK PIISLPDQKS 2150
    QPVSDSQERS SRVRRSGKKR KEPEGS 2176
    Length:2,176
    Mass (Da):240,431
    Last modified:May 1, 2000 - v1
    Checksum:i05DBD955D895C3D5
    GO
    Isoform 2 (identifier: Q9SGH2-2)

    Sequence is not available
    Length:
    Mass (Da):

    Sequence cautioni

    The sequence AAF01531.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC009325 Genomic DNA. Translation: AAF01531.1. Sequence problems.
    AC010870 Genomic DNA. Translation: AAF24616.1.
    CP002686 Genomic DNA. Translation: AEE73669.1.
    RefSeqiNP_186795.1. NM_111012.3. [Q9SGH2-1]
    UniGeneiAt.41275.
    At.47815.

    Genome annotation databases

    EnsemblPlantsiAT3G01460.1; AT3G01460.1; AT3G01460. [Q9SGH2-1]
    GeneIDi821132.
    KEGGiath:AT3G01460.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    The Chromatin Database (ChromDB)

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC009325 Genomic DNA. Translation: AAF01531.1 . Sequence problems.
    AC010870 Genomic DNA. Translation: AAF24616.1 .
    CP002686 Genomic DNA. Translation: AEE73669.1 .
    RefSeqi NP_186795.1. NM_111012.3. [Q9SGH2-1 ]
    UniGenei At.41275.
    At.47815.

    3D structure databases

    ProteinModelPortali Q9SGH2.
    SMRi Q9SGH2. Positions 82-133, 267-308, 1180-1240, 1290-1334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G01460.1-P.

    Proteomic databases

    PaxDbi Q9SGH2.
    PRIDEi Q9SGH2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G01460.1 ; AT3G01460.1 ; AT3G01460 . [Q9SGH2-1 ]
    GeneIDi 821132.
    KEGGi ath:AT3G01460.

    Organism-specific databases

    TAIRi AT3G01460.

    Phylogenomic databases

    eggNOGi NOG79337.
    HOGENOMi HOG000153462.
    InParanoidi Q9SGH2.
    OMAi FEEICSK.
    PhylomeDBi Q9SGH2.

    Gene expression databases

    Genevestigatori Q9SGH2.

    Family and domain databases

    Gene3Di 3.30.40.10. 2 hits.
    InterProi IPR016177. DNA-bd_dom.
    IPR003889. FYrich_C.
    IPR003888. FYrich_N.
    IPR001739. Methyl_CpG_DNA-bd.
    IPR028942. WHIM1_dom.
    IPR019786. Zinc_finger_PHD-type_CS.
    IPR011011. Znf_FYVE_PHD.
    IPR001965. Znf_PHD.
    IPR019787. Znf_PHD-finger.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF05965. FYRC. 1 hit.
    PF05964. FYRN. 1 hit.
    PF01429. MBD. 1 hit.
    PF00628. PHD. 2 hits.
    PF15612. WHIM1. 1 hit.
    [Graphical view ]
    SMARTi SM00249. PHD. 2 hits.
    SM00184. RING. 2 hits.
    [Graphical view ]
    SUPFAMi SSF54171. SSF54171. 1 hit.
    SSF57903. SSF57903. 2 hits.
    PROSITEi PS51543. FYRC. 1 hit.
    PS51542. FYRN. 1 hit.
    PS50982. MBD. 1 hit.
    PS01359. ZF_PHD_1. 2 hits.
    PS50016. ZF_PHD_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Ten members of the Arabidopsis gene family encoding methyl-CpG-binding domain proteins are transcriptionally active and at least one, AtMBD11, is crucial for normal development."
      Berg A., Meza T.J., Mahic M., Thorstensen T., Kristiansen K., Aalen R.B.
      Nucleic Acids Res. 31:5291-5304(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    4. "Evolutionary divergence of monocot and dicot methyl-CpG-binding domain proteins."
      Springer N.M., Kaeppler S.M.
      Plant Physiol. 138:92-104(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    5. "AtMBD9: a protein with a methyl-CpG-binding domain regulates flowering time and shoot branching in Arabidopsis."
      Peng M., Cui Y., Bi Y.-M., Rothstein S.J.
      Plant J. 46:282-296(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "Methyl-CpG-binding domain proteins in plants: interpreters of DNA methylation."
      Zemach A., Grafi G.
      Trends Plant Sci. 12:80-85(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    7. "AtMBD9 modulates Arabidopsis development through the dual epigenetic pathways of DNA methylation and histone acetylation."
      Yaish M.W.F., Peng M., Rothstein S.J.
      Plant J. 59:123-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMBD9_ARATH
    AccessioniPrimary (citable) accession number: Q9SGH2
    Secondary accession number(s): Q9SSA6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 8, 2011
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3