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Q9SGH2 (MBD9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-CpG-binding domain-containing protein 9

Short name=AtMBD9
Short name=MBD09
EC=2.3.1.48
Alternative name(s):
Histone acetyl transferase MBD9
Methyl-CpG-binding protein MBD9
Gene names
Name:MBD9
Ordered Locus Names:At3g01460
ORF Names:F4P13.1, T13O15.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length2176 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probable transcriptional regulator that acts as a histone acetyltransferase. Mediates the acetylation of histone H3 and H4 of target loci (e.g. FLC). Involved in an auxin-independent regulation of shoot branching and flowering time. Ref.5 Ref.7

Catalytic activity

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Subunit structure

Interacts with histone H4.

Subcellular location

Nucleus Ref.7.

Tissue specificity

Expressed in leaves, buds, flowers and stems. Ref.3 Ref.7

Domain

The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions By similarity.

Disruption phenotype

Over-methylated genomic DNA. Increased shoot branching and reduced transcription of FLC leading to early flowering, associated with a decrease in the acetylation level in histone H3 and H4 of FLC chromatin. Ref.5 Ref.7

Sequence similarities

Contains 1 bromo domain.

Contains 1 FYR C-terminal domain.

Contains 1 FYR N-terminal domain.

Contains 1 MBD (methyl-CpG-binding) domain.

Contains 2 PHD-type zinc fingers.

Contains 1 pumilio repeat.

Contains 2 RING-type zinc fingers.

Sequence caution

The sequence AAF01531.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]
Isoform 1 (identifier: Q9SGH2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9SGH2-2)

The sequence of this isoform is not available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 21762176Methyl-CpG-binding domain-containing protein 9
PRO_0000405285

Regions

Domain258 – 32770MBD
Domain403 – 45654FYR N-terminal
Domain550 – 698149FYR C-terminal
Repeat1098 – 113740Pumilio
Domain1157 – 122872Bromo
Zinc finger83 – 13351PHD-type 1
Zinc finger86 – 13146RING-type 1; degenerate
Zinc finger1287 – 133751PHD-type 2
Zinc finger1290 – 133546RING-type 2; degenerate
Coiled coil491 – 51121 Potential
Coiled coil1251 – 127323 Potential
Coiled coil1410 – 143728 Potential
Coiled coil1588 – 162841 Potential
Motif914 – 9218Nuclear localization signal By similarity
Motif1124 – 11318Nuclear localization signal By similarity
Motif1256 – 12638Nuclear localization signal By similarity
Motif1337 – 13448Nuclear localization signal By similarity
Motif1761 – 17688Nuclear localization signal By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 05DBD955D895C3D5

FASTA2,176240,431
        10         20         30         40         50         60 
MEPTDSTNEQ LGDTKTAAVK EESRSFLGID LNEIPTGATL GGGCTAGQDD DGEYEPVEVV 

        70         80         90        100        110        120 
RSIHDNPDPA PGAPAEVPEP DRDASCGACG RPESIELVVV CDACERGFHM SCVNDGVEAA 

       130        140        150        160        170        180 
PSADWMCSDC RTGGERSKLW PLGVKSKLIL DMNASPPSDA EGYGAEETSD SRKHMLASSS 

       190        200        210        220        230        240 
CIGNSFDYAM MHSSFSSLGR GHASLEASGL MSRNTKMSMD ALGSHNLGFG FPLNLNNSSL 

       250        260        270        280        290        300 
PMRFPSLDPS ELFLQNLRHF ISERHGVLED GWRVEFRQPL NGYQLCAVYC APNGKTFSSI 

       310        320        330        340        350        360 
QEVACYLGLA INGNYSCMDA EIRNENSLLQ ERLHTPKRRK TSRWPNNGFP EQKGSSVSAQ 

       370        380        390        400        410        420 
LRRFPFNGQT MSPFAVKSGT HFQAGGSLSS GNNGCGCEEA KNGCPMQFED FFVLSLGRID 

       430        440        450        460        470        480 
IRQSYHNVNV IYPIGYKSCW HDKITGSLFT CEVSDGNSGP IFKVTRSPCS KSFIPAGSTV 

       490        500        510        520        530        540 
FSCPKIDEMV EQNSDKLSNR RDSTQERDDD ASVEILLSEH CPPLGDDILS CLREKSFSKT 

       550        560        570        580        590        600 
VNSLRSEVDS SRVDFDKNLS YDQDHGVEIG DIVVEEDSLS DAWKKVSQKL VDACSIVLKQ 

       610        620        630        640        650        660 
KGTLNFLCKH VDRETSEINW DTMNEKDNVI LSLSKFCCSL APCSVTCGEK DKSEFAAVVD 

       670        680        690        700        710        720 
ALSRWLDQNR FGLDADFVQE MIEHMPGAES CTNYRTLKSR SSSSVPITVA EGALVVKPKG 

       730        740        750        760        770        780 
GENVKDEVFG EISRKAKKPK LNGGHGVRNL HPPPGRPMCL RLPPGLVGDF LQVSEVFWRF 

       790        800        810        820        830        840 
HEILGFEEAF SPENLEQELI NPVFDGLFLD KPGKDDKRSE INFTDKDSTA TKLFSLFDES 

       850        860        870        880        890        900 
RQPFPAKNTS ASELKEKKAG DSSDFKISDS SRGSCVGALL TRAHISLLQV LICELQSKVA 

       910        920        930        940        950        960 
AFVDPNFDSG ESRSRRGRKK DDSTLSAKRN KLHMLPVNEF TWPELARRYI LSLLSMDGNL 

       970        980        990       1000       1010       1020 
ESAEIAARES GKVFRCLQGD GGLLCGSLTG VAGMEADSML LAEAIKKISG SLTSENDVLS 

      1030       1040       1050       1060       1070       1080 
VEDDDSDGLD ATETNTCSGD IPEWAQVLEP VKKLPTNVGT RIRKCVYEAL ERNPPEWAKK 

      1090       1100       1110       1120       1130       1140 
ILEHSISKEI YKGNASGPTK KAVLSLLADI RGGDLVQRSI KGTKKRTYIS VSDVIMKKCR 

      1150       1160       1170       1180       1190       1200 
AVLRGVAAAD EDKVLCTLLG RKLLNSSDND DDGLLGSPAM VSRPLDFRTI DLRLAAGAYD 

      1210       1220       1230       1240       1250       1260 
GSTEAFLEDV LELWSSIRVM YADQPDCVDL VATLSEKFKS LYEAEVVPLV QKLKDYRKLE 

      1270       1280       1290       1300       1310       1320 
CLSAEMKKEI KDIVVSVNKL PKAPWDEGVC KVCGVDKDDD SVLLCDTCDA EYHTYCLNPP 

      1330       1340       1350       1360       1370       1380 
LIRIPDGNWY CPSCVIAKRM AQEALESYKL VRRRKGRKYQ GELTRASMEL TAHLADVMEE 

      1390       1400       1410       1420       1430       1440 
KDYWEFSAEE RILLLKLLCD ELLSSSLVHQ HLEQCAEAII EMQQKLRSLS SEWKNAKMRQ 

      1450       1460       1470       1480       1490       1500 
EFLTAKLAKV EPSILKEVGE PHNSSYFADQ MGCDPQPQEG VGDGVTRDDE TSSTAYLNKN 

      1510       1520       1530       1540       1550       1560 
QGKSPLETDT QPGESHVNFG ESKISSPETI SSPGRHELPI ADTSPLVTDN LPEKDTSETL 

      1570       1580       1590       1600       1610       1620 
LKSVGRNHET HSPNSNAVEL PTAHDASSQA SQELQACQQD LSATSNEIQN LQQSIRSIES 

      1630       1640       1650       1660       1670       1680 
QLLKQSIRRD FLGTDASGRL YWGCCFPDEN PRILVDGSIS LQKPVQADLI GSKVPSPFLH 

      1690       1700       1710       1720       1730       1740 
TVDHGRLRLS PWTYYETETE ISELVQWLHD DDLKERDLRE SILWWKRLRY GDVQKEKKQA 

      1750       1760       1770       1780       1790       1800 
QNLSAPVFAT GLETKAAMSM EKRYGPCIKL EMETLKKRGK KTKVAEREKL CRCECLESIL 

      1810       1820       1830       1840       1850       1860 
PSMIHCLICH KTFASDDEFE DHTESKCIPY SLATEEGKDI SDSSKAKESL KSDYLNVKSS 

      1870       1880       1890       1900       1910       1920 
AGKDVAEISN VSELDSGLIR YQEEESISPY HFEEICSKFV TKDCNRDLVK EIGLISSNGI 

      1930       1940       1950       1960       1970       1980 
PTFLPSSSTH LNDSVLISAK SNKPDGGDSG DQVIFAGPET NVEGLNSESN MSFDRSVTDS 

      1990       2000       2010       2020       2030       2040 
HGGPLDKPSG LGFGFSEQKN KKSSGSGLKS CCVVPQAALK RVTGKALPGF RFLKTNLLDM 

      2050       2060       2070       2080       2090       2100 
DVALPEEALR PSKSHPNRRR AWRVFVKSSQ SIYELVQATI VVEDMIKTEY LKNEWWYWSS 

      2110       2120       2130       2140       2150       2160 
LSAAAKISTL SALSVRIFSL DAAIIYDKPI TPSNPIDETK PIISLPDQKS QPVSDSQERS 

      2170 
SRVRRSGKKR KEPEGS 

« Hide

Isoform 2 (Sequence not available).

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Ten members of the Arabidopsis gene family encoding methyl-CpG-binding domain proteins are transcriptionally active and at least one, AtMBD11, is crucial for normal development."
Berg A., Meza T.J., Mahic M., Thorstensen T., Kristiansen K., Aalen R.B.
Nucleic Acids Res. 31:5291-5304(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[4]"Evolutionary divergence of monocot and dicot methyl-CpG-binding domain proteins."
Springer N.M., Kaeppler S.M.
Plant Physiol. 138:92-104(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[5]"AtMBD9: a protein with a methyl-CpG-binding domain regulates flowering time and shoot branching in Arabidopsis."
Peng M., Cui Y., Bi Y.-M., Rothstein S.J.
Plant J. 46:282-296(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]"Methyl-CpG-binding domain proteins in plants: interpreters of DNA methylation."
Zemach A., Grafi G.
Trends Plant Sci. 12:80-85(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[7]"AtMBD9 modulates Arabidopsis development through the dual epigenetic pathways of DNA methylation and histone acetylation."
Yaish M.W.F., Peng M., Rothstein S.J.
Plant J. 59:123-135(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC009325 Genomic DNA. Translation: AAF01531.1. Sequence problems.
AC010870 Genomic DNA. Translation: AAF24616.1.
CP002686 Genomic DNA. Translation: AEE73669.1.
RefSeqNP_186795.1. NM_111012.3.
UniGeneAt.41275.
At.47815.

3D structure databases

ProteinModelPortalQ9SGH2.
SMRQ9SGH2. Positions 84-133, 1174-1245, 1290-1334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G01460.1-P.

Proteomic databases

PaxDbQ9SGH2.
PRIDEQ9SGH2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G01460.1; AT3G01460.1; AT3G01460. [Q9SGH2-1]
GeneID821132.
KEGGath:AT3G01460.

Organism-specific databases

TAIRAT3G01460.

Phylogenomic databases

eggNOGNOG79337.
HOGENOMHOG000153462.
InParanoidQ9SGH2.
OMAICSKFVT.
PhylomeDBQ9SGH2.
ProtClustDBCLSN2684998.

Gene expression databases

GenevestigatorQ9SGH2.

Family and domain databases

Gene3D3.30.40.10. 2 hits.
InterProIPR016177. DNA-bd_dom.
IPR003889. FYrich_C.
IPR003888. FYrich_N.
IPR001739. Methyl_CpG_DNA-bd.
IPR028942. WHIM1_dom.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF05965. FYRC. 1 hit.
PF05964. FYRN. 1 hit.
PF01429. MBD. 1 hit.
PF00628. PHD. 2 hits.
PF15612. WHIM1. 1 hit.
[Graphical view]
SMARTSM00249. PHD. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
SUPFAMSSF54171. SSF54171. 1 hit.
SSF57903. SSF57903. 2 hits.
PROSITEPS51543. FYRC. 1 hit.
PS51542. FYRN. 1 hit.
PS50982. MBD. 1 hit.
PS01359. ZF_PHD_1. 2 hits.
PS50016. ZF_PHD_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMBD9_ARATH
AccessionPrimary (citable) accession number: Q9SGH2
Secondary accession number(s): Q9SSA6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names