Q9SGG3 (ALA5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative phospholipid-transporting ATPase 5
Short name=AtALA5
EC=3.6.3.1

Alternative name(s):
Aminophospholipid flippase 5

Gene names

Name:	ALA5
Ordered Locus Names:	At1g72700
ORF Names:	F28P22.11

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1228 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in transport of phospholipids Potential.
Catalytic activity	ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cation transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1228	1228	Putative phospholipid-transporting ATPase 5		PRO_0000046389
Regions
	Topological domain	1 – 74	74	Cytoplasmic Potential
	Transmembrane	75 – 96	22	Helical; Potential
	Topological domain	97 – 100	4	Extracellular Potential
	Transmembrane	101 – 123	23	Helical; Potential
	Topological domain	124 – 305	182	Cytoplasmic Potential
	Transmembrane	306 – 327	22	Helical; Potential
	Topological domain	328 – 359	32	Extracellular Potential
	Transmembrane	360 – 377	18	Helical; Potential
	Topological domain	378 – 934	557	Cytoplasmic Potential
	Transmembrane	935 – 954	20	Helical; Potential
	Topological domain	955 – 968	14	Extracellular Potential
	Transmembrane	969 – 988	20	Helical; Potential
	Topological domain	989 – 1018	30	Cytoplasmic Potential
	Transmembrane	1019 – 1041	23	Helical; Potential
	Topological domain	1042 – 1054	13	Extracellular Potential
	Transmembrane	1055 – 1077	23	Helical; Potential
	Topological domain	1078 – 1083	6	Cytoplasmic Potential
	Transmembrane	1084 – 1104	21	Helical; Potential
	Topological domain	1105 – 1117	13	Extracellular Potential
	Transmembrane	1118 – 1146	29	Helical; Potential
	Topological domain	1147 – 1228	82	Cytoplasmic Potential
Sites
	Active site	425	1	4-aspartylphosphate intermediate By similarity
	Metal binding	879	1	Magnesium By similarity
	Metal binding	883	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SGG3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 47E47A9297131CB8	FASTA	1,228	139,342
	10 20 30 40 50 60 MARGRIRSKL RLSLLYTFGC LRPATLEGQD SQPIQGPGFS RTVFCNQPHM HKKKPLRYRS 70 80 90 100 110 120 NYVSTTRYNL ITFFPKSLYE QFHRAANLYF LVAAILSVFP LSPFNKWSMI APLVFVVGLS 130 140 150 160 170 180 MLKEALEDWR RFMQDVKINA RKTCVHKSDG VFRQRKWKKV SVGDIVKVEK DEFFPADLLL 190 200 210 220 230 240 LSSSYEDGIC YVETMNLDGE TNLKVKRSLE VSLPLDDDES FKNFMATIRC EDPNPNLYTF 250 260 270 280 290 300 VGNLEFERQT FPLDPSQILL RDSKLRNTTY VYGVVVFTGF DTKVMQNSTK SPSKRSRIER 310 320 330 340 350 360 TMDYIIYTLL VLLILISCIS SSGFAWETEF HMPKMWYLRP GEPIDFTNPI NPIYAGVVHL 370 380 390 400 410 420 ITALLLYGYL IPISLYVSIE VVKVWQASFI NQDLHMYDDE SGVPANARTS NLNEELGQVH 430 440 450 460 470 480 TILSDKTGTL TCNQMDFLKC SIAGTSYGVR SSEVEVAAAK QMAVDLEEHG EISSTPQSQT 490 500 510 520 530 540 KVYGTWDSSR TQEIEVEGDN NYNTPRAPIK GFGFEDNRLM NGNWLRESQP NDILQFFRIL 550 560 570 580 590 600 AICHTAIPEL NEETGKYTYE AESPDEASFL AAAREFGFEF FKRTQSSVFI RERFSGSGQI 610 620 630 640 650 660 IEREYKVLNL LEFTSKRKRM TVIVRDEEGQ ILLLCKGADS IIFERLAKNG KTYLGPTTRH 670 680 690 700 710 720 LTEYGEAGLR TLALAYRKLD EDEYAAWNSE FLKAKTSIGS DRDELLETGA DMIEKELILI 730 740 750 760 770 780 GATAVEDKLQ KGVPQCIDKL AQAGLKLWVL TGDKMETAIN IGFACSLLRQ GMRQICITSM 790 800 810 820 830 840 NSEGGSQDSK RVVKENILNQ LTKAVQMVKL EKDPHAAFAL IIDGKTLTYA LEDDMKYQFL 850 860 870 880 890 900 ALAVDCASVI CCRVSPKQKA LVVRLVKEGT GKTTLAIGDG ANDVGMIQEA DIGVGISGVE 910 920 930 940 950 960 GMQAVMASDF SIAQFRFLER LLVVHGHWCY KRIAQMICYF FYKNIAFGLT LFYFEAFTGF 970 980 990 1000 1010 1020 SGQSVYNDYY LLLFNVVLTS LPVIALGVFE QDVSSEICLQ FPALYQQGTK NLFFDWSRIL 1030 1040 1050 1060 1070 1080 GWMCNGVYAS LVIFFLNIGI IYSQAFRDNG QTADMDAVGT TMFTCIIWAA NVQIALTMSH 1090 1100 1110 1120 1130 1140 FTWIQHVLIW GSIGMWYLFV AIYSMMPPSY SGNIYRILDE ILAPAPIYWM ATLLVTVAAV 1150 1160 1170 1180 1190 1200 LPYVAHIAFQ RFLNPLDHHI IQEIKYYGRD IEDARLWTRE RTKAREKTKI GFTARVDAKI 1210 1220 RHLRSKLNKK QSNLSHFSAQ DAMSPRSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC010926 Genomic DNA. Translation: AAG51844.1. CP002684 Genomic DNA. Translation: AEE35362.1.
IPI	IPI00528116.
PIR	G96751.
RefSeq	NP_177414.1. NM_105929.4.
UniGene	At.35067.
3D structure databases
ProteinModelPortal	Q9SGG3.
SMR	Q9SGG3. Positions 851-914.
ModBase	Search...
Proteomic databases
PRIDE	Q9SGG3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G72700.1; AT1G72700.1; AT1G72700.
GeneID	843602.
GenomeReviews	Gene locus AT1G72700 in contig CT485782_GR.
KEGG	ath:AT1G72700.
NMPDR	fig|3702.1.peg.6763.
Organism-specific databases
TAIR	At1g72700.
Phylogenomic databases
eggNOG	KOG0206.
GeneTree	EPGT00070000028188.
HOGENOM	HBG745019.
InParanoid	Q9SGG3.
OMA	NRSHGEK.
PhylomeDB	Q9SGG3.
ProtClustDB	CLSN2679593.
Gene expression databases
ArrayExpress	Q9SGG3.
Genevestigator	Q9SGG3.
GermOnline	AT1G72700. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR023306. ATPase_cation_domN. IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 2 hits. G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K01530.
Pfam	PF00122. E1-E2_ATPase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 1 hit.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALA5_ARATH

Accession

Primary (citable) accession number: Q9SGG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program