Reviewed, UniProtKB/Swiss-Prot Q9SGD6 (AROD6_ARATH)

Names and origin

Protein names

Recommended name:
    Arogenate dehydratase/prephenate dehydratase 6, chloroplastic
      Short name=AtADT6
      Short name=AtPDT6
    EC=4.2.1.91
    EC=4.2.1.51

Gene names

Name:	ADT6
Synonyms:	PDT6
Ordered Locus Names:	At1g08250
ORF Names:	T23G18.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine. Ref.4
Catalytic activity	L-arogenate = L-phenylalanine + H2O + CO2. Prephenate = phenylpyruvate + H2O + CO2.
Pathway	Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-phenylalanine from L-arogenate: step 1/1. Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.
Subcellular location	Plastid › chloroplast stroma Ref.5.
Tissue specificity	Expressed in roots, leaves, stems, flowers and siliques. Ref.4
Sequence similarities	Contains 1 prephenate dehydratase domain.
Biophysicochemical properties	Kinetic parameters: KM=2.58 mM for arogenate KM=2.44 mM for prephenate Vmax=42.61 pmol/sec/µg enzyme with arogenate as substrate Vmax=0.4 pmol/sec/µg enzyme with prephenate as substrate

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Phenylalanine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arogenate dehydratase activity Ref.4 Inferred from direct assay. Source: TAIR prephenate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 44	44	Chloroplast Potential
	Chain	45 – 413	369	Arogenate dehydratase/prephenate dehydratase 6, chloroplastic		PRO_0000373795
Regions
	Domain	117 – 294	178	Prephenate dehydratase

Sequences

	Sequence		Length	Mass (Da)
	Q9SGD6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 70099DC47A932022	FASTA	413	44,802
	10 20 30 40 50 60 MKALSSSSPI LGASQPATAT ALIARSGRSE WQSSCAILTS KVISQEESES LPVPPVSGGV 70 80 90 100 110 120 DHLNGHNSAA ARVPGMNLVP IEKSDSNPLV PQHRHNPLKP LSMTDLSPAP MHGSNLRVAY 130 140 150 160 170 180 QGVPGAYSEA AAGKAYPNCQ AIPCDQFEVA FQAVELWIAD RAVLPVENSL GGSIHRNYDL 190 200 210 220 230 240 LLRHRLHIVG EVQLPVHHCL LALPGVRKEF LTRVISHPQG LAQCEHTLTK LGLNVAREAV 250 260 270 280 290 300 DDTAGAAEFI ASNNLRDTAA IASARAAEIY GLEILEDGIQ DDVSNVTRFV MLAREPIIPR 310 320 330 340 350 360 TDRPFKTSIV FAHEKGTSVL FKVLSAFAFR DISLTKIESR PNHNRPIRVV DDANVGTAKH 370 380 390 400 410 FEYMFYVDFE ASMAEARAQN ALAEVQEFTS FLRVLGSYPM DMTPWSPTSS TSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Matringe M., Grisollet D., Rippert P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Phenylalanine biosynthesis in Arabidopsis thaliana. Identification and characterization of arogenate dehydratases." Cho M.-H., Corea O.R.A., Yang H., Bedgar D.L., Laskar D.D., Anterola A.M., Moog-Anterola F.A., Hood R.L., Kohalmi S.E., Bernards M.A., Kang C., Davin L.B., Lewis N.G. J. Biol. Chem. 282:30827-30835(2007) [PubMed: 17726025] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
[5]	"Tyrosine and phenylalanine are synthesized within the plastids in Arabidopsis." Rippert P., Puyaubert J., Grisollet D., Derrier L., Matringe M. Plant Physiol. 149:1251-1260(2009) [PubMed: 19136569] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ411468 mRNA. Translation: ABD67754.1. AC011438 Genomic DNA. Translation: AAF18250.1. AY056290 mRNA. Translation: AAL07139.1. AY091181 mRNA. Translation: AAM14120.1.
IPI	IPI00523853.
PIR	E86216.
RefSeq	NP_563809.1.
UniGene	At.16611
3D structure databases
SMR	Q9SGD6. Positions 115-401.
ModBase	Search...
Proteomic databases
PRIDE	Q9SGD6.
Genome annotation databases
GeneID	837345.
GenomeReviews	Gene locus AT1G08250 in contig CT485782_GR.
KEGG	ath:AT1G08250.
NMPDR	fig|3702.1.peg.1031.
Organism-specific databases
TAIR	At1g08250.
Phylogenomic databases
eggNOG	KOG2797.
HOGENOM	HBG693866.
InParanoid	Q9SGD6.
OMA	NANIERI.
PhylomeDB	Q9SGD6.
Gene expression databases
ArrayExpress	Q9SGD6.
Genevestigator	Q9SGD6.
Family and domain databases
InterPro	IPR001086. Preph_deHydtase. IPR018528. Preph_deHydtase_CS. [Graphical view]
Pfam	PF00800. PDT. 1 hit. [Graphical view]
PROSITE	PS00857. PREPHENATE_DEHYDR_1. 1 hit. PS00858. PREPHENATE_DEHYDR_2. 1 hit. PS51171. PREPHENATE_DEHYDR_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD6_ARATH

Accession

Primary (citable) accession number: Q9SGD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)