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Protein

Mannan endo-1,4-beta-mannosidase 3

Gene

MAN3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei203Proton donorBy similarity1
Active sitei323NucleophileBy similarity1

GO - Molecular functioni

  • mannan endo-1,4-beta-mannosidase activity Source: TAIR

GO - Biological processi

  • detoxification of cadmium ion Source: TAIR
  • mannan catabolic process Source: GO_Central
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT3G10890-MONOMER

Protein family/group databases

CAZyiGH5 Glycoside Hydrolase Family 5

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 3 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 3
Endo-beta-1,4-mannanase 3
Short name:
AtMAN3
Gene namesi
Name:MAN3
Ordered Locus Names:At3g10890
ORF Names:T7M13.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G10890
TAIRilocus:2103262 AT3G10890

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000027747620 – 414Mannan endo-1,4-beta-mannosidase 3Add BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi17N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi133N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi343N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi386N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SG94

Expressioni

Tissue specificityi

Expressed in leaves, flowers, siliques and seeds.1 Publication

Gene expression databases

ExpressionAtlasiQ9SG94 baseline and differential
GenevisibleiQ9SG94 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G10890.1

Structurei

3D structure databases

ProteinModelPortaliQ9SG94
SMRiQ9SG94
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHRI Eukaryota
COG3934 LUCA
HOGENOMiHOG000169952
InParanoidiQ9SG94
KOiK19355
OMAiMVNRVNT
OrthoDBiEOG09360BHK
PhylomeDBiQ9SG94

Family and domain databases

InterProiView protein in InterPro
IPR001547 Glyco_hydro_5
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00150 Cellulase, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SG94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCLCFVVLL AILIAQNSSD LGVKSASSDG FVSRKGVQFI LNGKPFYANG
60 70 80 90 100
FNAYWLAYEA TDSTTRFKIT YVFQNATIHD LTIVRTWGFR DGGYRALQIA
110 120 130 140 150
PGVYDEKTFQ GLDFAIAEAK RLGIKMIITF VNNYSDFGGR KQYVDWAKNT
160 170 180 190 200
GQNVSSDDDF YTNPLVKQYY KNHVKTMVNR VNTFTKVEYK DEPTIMGWEL
210 220 230 240 250
MNEPQCRADP SGKTLTAWMN EMALYVKSVD SKHLLSTGLE GFYGDSSPQR
260 270 280 290 300
KTSLNPVAAN VLGTDFIANH KLDAIDFASI HSYPDLWFPN LDEKSRLNLL
310 320 330 340 350
RKWLECHLED AQNILKKPLI LGEFGKPTNT PGYTQAQRDA VFNATFDTIY
360 370 380 390 400
ESAEKGGPAA GALFWHVISD GMNNFKDPLS IVLSENSTTV NIITEESRKL
410
GLIRGKGKLS KTKI
Length:414
Mass (Da):46,462
Last modified:May 1, 2000 - v1
Checksum:iBBACD11830EB2609
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011708 Genomic DNA Translation: AAF19560.1
CP002686 Genomic DNA Translation: AEE74967.1
RefSeqiNP_187700.1, NM_111926.2
UniGeneiAt.39840

Genome annotation databases

EnsemblPlantsiAT3G10890.1; AT3G10890.1; AT3G10890
GeneIDi820259
GrameneiAT3G10890.1; AT3G10890.1; AT3G10890
KEGGiath:AT3G10890

Similar proteinsi

Entry informationi

Entry nameiMAN3_ARATH
AccessioniPrimary (citable) accession number: Q9SG94
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health