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Q9SG80

- ASD1_ARATH

UniProt

Q9SG80 - ASD1_ARATH

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Protein

Alpha-L-arabinofuranosidase 1

Gene

ASD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in the coordinated dissolution of the cell wall matrix during abscission and in the secondary cell wall formation in xylem vessels. Prefers arabinoxylan, but may also use pectic arabinans as substrates.3 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.2 Publications

Kineticsi

  1. KM=0.48 mM for p-nitrophenyl-beta-D-xylopyranoside2 Publications
  2. KM=5.2 mM for (1,5)-alpha-L-arabinobiose (at 37 degrees Celsius)2 Publications

pH dependencei

Optimum pH is 4.3.2 Publications

Temperature dependencei

Optimum temperature is 67 degrees Celsius.2 Publications

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: TAIR
  2. xylan 1,4-beta-xylosidase activity Source: TAIR

GO - Biological processi

  1. L-arabinose metabolic process Source: InterPro
  2. xylan catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciARA:AT3G10740-MONOMER.
SABIO-RKQ9SG80.

Protein family/group databases

CAZyiGH51. Glycoside Hydrolase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-L-arabinofuranosidase 1 (EC:3.2.1.55)
Short name:
AtASD1
Alternative name(s):
Beta-D-xylosidase (EC:3.2.1.-)
Gene namesi
Name:ASD1
Synonyms:ARAF, ARAF1
Ordered Locus Names:At3g10740
ORF Names:T7M13.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G10740.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. plant-type cell wall Source: TAIR
  3. proteinaceous extracellular matrix Source: UniProtKB-KW
  4. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; even in asd1 and asd2 double mutant.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Chaini34 – 678645Alpha-L-arabinofuranosidase 1PRO_0000384371Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi555 – 5551N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SG80.
PRIDEiQ9SG80.

Expressioni

Tissue specificityi

Expressed in roots, leaves, flowers, stems, siliques and seedlings. Observed in zones of cell proliferation, the vascular system and floral abscission zones. Expressed in the guard cells in stems, in xylem vessels and parenchyma cells surrounding the vessels, in the cambium and in the phloem, but not in the secondary xylem.3 Publications

Inductioni

Not induced by hormones or during leaf senescence.1 Publication

Gene expression databases

GenevestigatoriQ9SG80.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G10740.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SG80.
SMRiQ9SG80. Positions 229-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini152 – 23988CBM-cenCAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 51 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3534.
HOGENOMiHOG000115340.
InParanoidiQ9SG80.
KOiK01209.
OMAiCITHGLG.
PhylomeDBiQ9SG80.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR010720. Alpha-L-AF_C.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF06964. Alpha-L-AF_C. 1 hit.
[Graphical view]
SMARTiSM00813. Alpha-L-AF_C. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SG80-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDMESWKLLR SVCVLSFLLG SCFVYQSLRV VDAQEDPKPA VTLQVDASNG
60 70 80 90 100
GGRPIPETLF GIFFEEINHA GAGGLWAELV SNRGFEAGGQ NTPSNIWPWS
110 120 130 140 150
IVGDHSSIYV ATDRSSCFER NKIALRMDVL CDSKGCPSGG VGVYNPGYWG
160 170 180 190 200
MNIEEGKKYK VALYVRSTGD IDLSVSLTSS NGSRTLASEK IIASASDVSK
210 220 230 240 250
WIKKEVLLEA KATDPSARLQ LTTTKKGSIW IDQVSAMPVD THKGHGFRND
260 270 280 290 300
LFQMMADIKP RFIRFPGGCF VEGEWLSNAF RWKETVGPWE ERPGHFGDVW
310 320 330 340 350
KYWTDDGLGH FEFFQMAEDI GAAPIWVFNN GISHNDEVET ASIMPFVQEA
360 370 380 390 400
LDGIEFARGD ANSTWGSVRA KMGRQEPFEL KYVAIGNEDC GKTYYRGNYI
410 420 430 440 450
VFYDAIKKAY PDIKIISNCD GSSHPLDHPA DYYDYHIYTS ASNLFSMYHQ
460 470 480 490 500
FDRTSRKGPK AFVSEYAVTG KDAGTGSLLA SLAEAAFLIG LEKNSDIVEM
510 520 530 540 550
ASYAPLFVNT NDRRWNPDAI VFNSSHLYGT PSYWVQRFFA ESSGATLLTS
560 570 580 590 600
TLKGNSTSLV ASAISWKNNG KDYIRIKAVN FGANSENMQV LVTGLDPNVM
610 620 630 640 650
RVSGSKKTVL TSTNVMDENS FSQPEKVVPH ESLLELAEED MTVVLPPHSF
660 670
SSFDLLKESA KIRMPISDSS SHQKTTTV
Length:678
Mass (Da):75,045
Last modified:May 1, 2000 - v1
Checksum:i8116546072E6CA9A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti251 – 2511L → P in AAN28883. (PubMed:14593172)Curated
Sequence conflicti251 – 2511L → P in AAK50089. (PubMed:14593172)Curated
Sequence conflicti253 – 2542QM → HL in AAO92261. (PubMed:14512381)Curated
Sequence conflicti453 – 4531R → G in AAO92261. (PubMed:14512381)Curated
Sequence conflicti499 – 4991E → D in AAO92261. (PubMed:14512381)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY243509 mRNA. Translation: AAO92261.1.
AC011708 Genomic DNA. Translation: AAF19575.1.
CP002686 Genomic DNA. Translation: AEE74949.1.
AF372949 mRNA. Translation: AAK50089.1.
AY143944 mRNA. Translation: AAN28883.1.
AK222175 mRNA. Translation: BAD95302.1.
AK220766 mRNA. Translation: BAD93969.1.
RefSeqiNP_187685.1. NM_111911.4.
UniGeneiAt.20271.

Genome annotation databases

EnsemblPlantsiAT3G10740.1; AT3G10740.1; AT3G10740.
GeneIDi820243.
KEGGiath:AT3G10740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY243509 mRNA. Translation: AAO92261.1 .
AC011708 Genomic DNA. Translation: AAF19575.1 .
CP002686 Genomic DNA. Translation: AEE74949.1 .
AF372949 mRNA. Translation: AAK50089.1 .
AY143944 mRNA. Translation: AAN28883.1 .
AK222175 mRNA. Translation: BAD95302.1 .
AK220766 mRNA. Translation: BAD93969.1 .
RefSeqi NP_187685.1. NM_111911.4.
UniGenei At.20271.

3D structure databases

ProteinModelPortali Q9SG80.
SMRi Q9SG80. Positions 229-559.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G10740.1-P.

Protein family/group databases

CAZyi GH51. Glycoside Hydrolase Family 51.

Proteomic databases

PaxDbi Q9SG80.
PRIDEi Q9SG80.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G10740.1 ; AT3G10740.1 ; AT3G10740 .
GeneIDi 820243.
KEGGi ath:AT3G10740.

Organism-specific databases

TAIRi AT3G10740.

Phylogenomic databases

eggNOGi COG3534.
HOGENOMi HOG000115340.
InParanoidi Q9SG80.
KOi K01209.
OMAi CITHGLG.
PhylomeDBi Q9SG80.

Enzyme and pathway databases

BioCyci ARA:AT3G10740-MONOMER.
SABIO-RK Q9SG80.

Gene expression databases

Genevestigatori Q9SG80.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
InterProi IPR010720. Alpha-L-AF_C.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF06964. Alpha-L-AF_C. 1 hit.
[Graphical view ]
SMARTi SM00813. Alpha-L-AF_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two alpha-L-arabinofuranosidase genes in Arabidopsis thaliana are differentially expressed during vegetative growth and flower development."
    Fulton L.M., Cobbett C.S.
    J. Exp. Bot. 54:2467-2477(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY HORMONES.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 287-678.
    Strain: cv. Columbia.
  6. "Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis."
    Minic Z., Rihouey C., Do C.T., Lerouge P., Jouanin L.
    Plant Physiol. 135:867-878(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  7. "Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis."
    Minic Z., Do C.-T., Rihouey C., Morin H., Lerouge P., Jouanin L.
    J. Exp. Bot. 57:2339-2351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  8. "Cell wall modifications in Arabidopsis plants with altered alpha-L-arabinofuranosidase activity."
    Chavez Montes R.A., Ranocha P., Martinez Y., Minic Z., Jouanin L., Marquis M., Saulnier L., Fulton L.M., Cobbett C.S., Bitton F., Renou J.-P., Jauneau A., Goffner D.
    Plant Physiol. 147:63-77(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiASD1_ARATH
AccessioniPrimary (citable) accession number: Q9SG80
Secondary accession number(s): Q56W72
, Q570E2, Q84RC5, Q94JT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3