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Q9SG80 (ASD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-L-arabinofuranosidase 1

Short name=AtASD1
EC=3.2.1.55
Alternative name(s):
Beta-D-xylosidase
EC=3.2.1.-
Gene names
Name:ASD1
Synonyms:ARAF, ARAF1
Ordered Locus Names:At3g10740
ORF Names:T7M13.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length678 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in the coordinated dissolution of the cell wall matrix during abscission and in the secondary cell wall formation in xylem vessels. Prefers arabinoxylan, but may also use pectic arabinans as substrates. Ref.6 Ref.7 Ref.8

Catalytic activity

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides. Ref.6 Ref.7

Subcellular location

Secretedextracellular spaceextracellular matrix Potential.

Tissue specificity

Expressed in roots, leaves, flowers, stems, siliques and seedlings. Observed in zones of cell proliferation, the vascular system and floral abscission zones. Expressed in the guard cells in stems, in xylem vessels and parenchyma cells surrounding the vessels, in the cambium and in the phloem, but not in the secondary xylem. Ref.1 Ref.6 Ref.8

Induction

Not induced by hormones or during leaf senescence. Ref.1

Disruption phenotype

No visible phenotype; even in asd1 and asd2 double mutant. Ref.8

Sequence similarities

Belongs to the glycosyl hydrolase 51 family.

Contains 1 CBM-cenC (cenC-type cellulose-binding) domain.

Biophysicochemical properties

Kinetic parameters:

KM=0.48 mM for p-nitrophenyl-beta-D-xylopyranoside Ref.6 Ref.7

KM=5.2 mM for (1,5)-alpha-L-arabinobiose (at 37 degrees Celsius)

pH dependence:

Optimum pH is 4.3.

Temperature dependence:

Optimum temperature is 67 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 678645Alpha-L-arabinofuranosidase 1
PRO_0000384371

Regions

Domain152 – 23988CBM-cenC

Amino acid modifications

Glycosylation1811N-linked (GlcNAc...) Potential
Glycosylation3621N-linked (GlcNAc...) Potential
Glycosylation5231N-linked (GlcNAc...) Potential
Glycosylation5551N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2511L → P in AAN28883. Ref.4
Sequence conflict2511L → P in AAK50089. Ref.4
Sequence conflict253 – 2542QM → HL in AAO92261. Ref.1
Sequence conflict4531R → G in AAO92261. Ref.1
Sequence conflict4991E → D in AAO92261. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9SG80 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 8116546072E6CA9A

FASTA67875,045
        10         20         30         40         50         60 
MDMESWKLLR SVCVLSFLLG SCFVYQSLRV VDAQEDPKPA VTLQVDASNG GGRPIPETLF 

        70         80         90        100        110        120 
GIFFEEINHA GAGGLWAELV SNRGFEAGGQ NTPSNIWPWS IVGDHSSIYV ATDRSSCFER 

       130        140        150        160        170        180 
NKIALRMDVL CDSKGCPSGG VGVYNPGYWG MNIEEGKKYK VALYVRSTGD IDLSVSLTSS 

       190        200        210        220        230        240 
NGSRTLASEK IIASASDVSK WIKKEVLLEA KATDPSARLQ LTTTKKGSIW IDQVSAMPVD 

       250        260        270        280        290        300 
THKGHGFRND LFQMMADIKP RFIRFPGGCF VEGEWLSNAF RWKETVGPWE ERPGHFGDVW 

       310        320        330        340        350        360 
KYWTDDGLGH FEFFQMAEDI GAAPIWVFNN GISHNDEVET ASIMPFVQEA LDGIEFARGD 

       370        380        390        400        410        420 
ANSTWGSVRA KMGRQEPFEL KYVAIGNEDC GKTYYRGNYI VFYDAIKKAY PDIKIISNCD 

       430        440        450        460        470        480 
GSSHPLDHPA DYYDYHIYTS ASNLFSMYHQ FDRTSRKGPK AFVSEYAVTG KDAGTGSLLA 

       490        500        510        520        530        540 
SLAEAAFLIG LEKNSDIVEM ASYAPLFVNT NDRRWNPDAI VFNSSHLYGT PSYWVQRFFA 

       550        560        570        580        590        600 
ESSGATLLTS TLKGNSTSLV ASAISWKNNG KDYIRIKAVN FGANSENMQV LVTGLDPNVM 

       610        620        630        640        650        660 
RVSGSKKTVL TSTNVMDENS FSQPEKVVPH ESLLELAEED MTVVLPPHSF SSFDLLKESA 

       670 
KIRMPISDSS SHQKTTTV 

« Hide

References

« Hide 'large scale' references
[1]"Two alpha-L-arabinofuranosidase genes in Arabidopsis thaliana are differentially expressed during vegetative growth and flower development."
Fulton L.M., Cobbett C.S.
J. Exp. Bot. 54:2467-2477(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY HORMONES.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 287-678.
Strain: cv. Columbia.
[6]"Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis."
Minic Z., Rihouey C., Do C.T., Lerouge P., Jouanin L.
Plant Physiol. 135:867-878(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[7]"Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis."
Minic Z., Do C.-T., Rihouey C., Morin H., Lerouge P., Jouanin L.
J. Exp. Bot. 57:2339-2351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[8]"Cell wall modifications in Arabidopsis plants with altered alpha-L-arabinofuranosidase activity."
Chavez Montes R.A., Ranocha P., Martinez Y., Minic Z., Jouanin L., Marquis M., Saulnier L., Fulton L.M., Cobbett C.S., Bitton F., Renou J.-P., Jauneau A., Goffner D.
Plant Physiol. 147:63-77(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY243509 mRNA. Translation: AAO92261.1.
AC011708 Genomic DNA. Translation: AAF19575.1.
CP002686 Genomic DNA. Translation: AEE74949.1.
AF372949 mRNA. Translation: AAK50089.1.
AY143944 mRNA. Translation: AAN28883.1.
AK222175 mRNA. Translation: BAD95302.1.
AK220766 mRNA. Translation: BAD93969.1.
RefSeqNP_187685.1. NM_111911.4.
UniGeneAt.20271.

3D structure databases

ProteinModelPortalQ9SG80.
SMRQ9SG80. Positions 229-559.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G10740.1-P.

Protein family/group databases

CAZyGH51. Glycoside Hydrolase Family 51.

Proteomic databases

PaxDbQ9SG80.
PRIDEQ9SG80.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G10740.1; AT3G10740.1; AT3G10740.
GeneID820243.
KEGGath:AT3G10740.

Organism-specific databases

TAIRAT3G10740.

Phylogenomic databases

eggNOGCOG3534.
HOGENOMHOG000115340.
InParanoidQ9SG80.
KOK01209.
OMACITHGLG.
PhylomeDBQ9SG80.

Enzyme and pathway databases

BioCycARA:AT3G10740-MONOMER.
SABIO-RKQ9SG80.

Gene expression databases

GenevestigatorQ9SG80.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
InterProIPR010720. Alpha-L-AF_C.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF06964. Alpha-L-AF_C. 1 hit.
[Graphical view]
SMARTSM00813. Alpha-L-AF_C. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameASD1_ARATH
AccessionPrimary (citable) accession number: Q9SG80
Secondary accession number(s): Q56W72 expand/collapse secondary AC list , Q570E2, Q84RC5, Q94JT7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names