Alpha-L-arabinofuranosidase 1 precursor - Arabidopsis thaliana (Mouse-ear cress)

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Q9SG80 (ASD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 72. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-L-arabinofuranosidase 1
Short name=AtASD1
EC=3.2.1.55

Alternative name(s):
Beta-D-xylosidase
EC=3.2.1.-

Gene names

Name:	ASD1
Synonyms:	ARAF, ARAF1
Ordered Locus Names:	At3g10740
ORF Names:	T7M13.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the coordinated dissolution of the cell wall matrix during abscission and in the secondary cell wall formation in xylem vessels. Prefers arabinoxylan, but may also use pectic arabinans as substrates. Ref.6 Ref.7 Ref.8
Catalytic activity	Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides. Ref.6 Ref.7
Subcellular location	Secreted › extracellular space › extracellular matrix Potential.
Tissue specificity	Expressed in roots, leaves, flowers, stems, siliques and seedlings. Observed in zones of cell proliferation, the vascular system and floral abscission zones. Expressed in the guard cells in stems, in xylem vessels and parenchyma cells surrounding the vessels, in the cambium and in the phloem, but not in the secondary xylem. Ref.1 Ref.6 Ref.8
Induction	Not induced by hormones or during leaf senescence. Ref.1
Disruption phenotype	No developmental phenotype, even in a asd1/asd2 double mutant. Ref.8
Sequence similarities	Belongs to the glycosyl hydrolase 51 family. Contains 1 CBM-cenC (cenC-type cellulose-binding) domain.
Biophysicochemical properties	Kinetic parameters: K_M=0.48 mM for p-nitrophenyl-beta-D-xylopyranoside Ref.6 Ref.7 K_M=5.2 mM for (1,5)-alpha-L-arabinobiose (at 37 degrees Celsius) pH dependence: Optimum pH is 4.3. Temperature dependence: Optimum temperature is 67 degrees Celsius.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-arabinose metabolic process Inferred from electronic annotation. Source: InterPro xylan catabolic process Inferred from direct assay Ref.6. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR plant-type cell wall Inferred from direct assay PubMed 17526915. Source: TAIR proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from direct assay PubMed 15539469. Source: TAIR
Molecular_function	alpha-N-arabinofuranosidase activity Inferred from direct assay Ref.6. Source: TAIR xylan 1,4-beta-xylosidase activity Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 33

Potential

Chain

34 – 678

645

Alpha-L-arabinofuranosidase 1

PRO_0000384371

Regions

Domain

152 – 239

CBM-cenC

Amino acid modifications

Glycosylation

181

N-linked (GlcNAc...) Potential

Glycosylation

362

N-linked (GlcNAc...) Potential

Glycosylation

523

N-linked (GlcNAc...) Potential

Glycosylation

555

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

251

L → P in AAN28883. Ref.4

Sequence conflict

251

L → P in AAK50089. Ref.4

Sequence conflict

253 – 254

QM → HL in AAO92261. Ref.1

Sequence conflict

453

R → G in AAO92261. Ref.1

Sequence conflict

499

E → D in AAO92261. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9SG80 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 8116546072E6CA9A
Show »

FASTA

678

75,045

        10         20         30         40         50         60 
MDMESWKLLR SVCVLSFLLG SCFVYQSLRV VDAQEDPKPA VTLQVDASNG GGRPIPETLF 

        70         80         90        100        110        120 
GIFFEEINHA GAGGLWAELV SNRGFEAGGQ NTPSNIWPWS IVGDHSSIYV ATDRSSCFER 

       130        140        150        160        170        180 
NKIALRMDVL CDSKGCPSGG VGVYNPGYWG MNIEEGKKYK VALYVRSTGD IDLSVSLTSS 

       190        200        210        220        230        240 
NGSRTLASEK IIASASDVSK WIKKEVLLEA KATDPSARLQ LTTTKKGSIW IDQVSAMPVD 

       250        260        270        280        290        300 
THKGHGFRND LFQMMADIKP RFIRFPGGCF VEGEWLSNAF RWKETVGPWE ERPGHFGDVW 

       310        320        330        340        350        360 
KYWTDDGLGH FEFFQMAEDI GAAPIWVFNN GISHNDEVET ASIMPFVQEA LDGIEFARGD 

       370        380        390        400        410        420 
ANSTWGSVRA KMGRQEPFEL KYVAIGNEDC GKTYYRGNYI VFYDAIKKAY PDIKIISNCD 

       430        440        450        460        470        480 
GSSHPLDHPA DYYDYHIYTS ASNLFSMYHQ FDRTSRKGPK AFVSEYAVTG KDAGTGSLLA 

       490        500        510        520        530        540 
SLAEAAFLIG LEKNSDIVEM ASYAPLFVNT NDRRWNPDAI VFNSSHLYGT PSYWVQRFFA 

       550        560        570        580        590        600 
ESSGATLLTS TLKGNSTSLV ASAISWKNNG KDYIRIKAVN FGANSENMQV LVTGLDPNVM 

       610        620        630        640        650        660 
RVSGSKKTVL TSTNVMDENS FSQPEKVVPH ESLLELAEED MTVVLPPHSF SSFDLLKESA 

       670 
KIRMPISDSS SHQKTTTV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two alpha-L-arabinofuranosidase genes in Arabidopsis thaliana are differentially expressed during vegetative growth and flower development." Fulton L.M., Cobbett C.S. J. Exp. Bot. 54:2467-2477(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY HORMONES. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 287-678. Strain: cv. Columbia.
[6]	"Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis." Minic Z., Rihouey C., Do C.T., Lerouge P., Jouanin L. Plant Physiol. 135:867-878(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[7]	"Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis." Minic Z., Do C.-T., Rihouey C., Morin H., Lerouge P., Jouanin L. J. Exp. Bot. 57:2339-2351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[8]	"Cell wall modifications in Arabidopsis plants with altered alpha-L-arabinofuranosidase activity." Chavez Montes R.A., Ranocha P., Martinez Y., Minic Z., Jouanin L., Marquis M., Saulnier L., Fulton L.M., Cobbett C.S., Bitton F., Renou J.-P., Jauneau A., Goffner D. Plant Physiol. 147:63-77(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY243509 mRNA. Translation: AAO92261.1. AC011708 Genomic DNA. Translation: AAF19575.1. CP002686 Genomic DNA. Translation: AEE74949.1. AF372949 mRNA. Translation: AAK50089.1. AY143944 mRNA. Translation: AAN28883.1. AK222175 mRNA. Translation: BAD95302.1. AK220766 mRNA. Translation: BAD93969.1.
IPI	IPI00526411.
RefSeq	NP_187685.1. NM_111911.4.
UniGene	At.20271.
3D structure databases
ProteinModelPortal	Q9SG80.
SMR	Q9SG80. Positions 229-559.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G10740.1-P.
Protein family/group databases
CAZy	GH51. Glycoside Hydrolase Family 51.
Proteomic databases
PaxDb	Q9SG80.
PRIDE	Q9SG80.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G10740.1; AT3G10740.1; AT3G10740.
GeneID	820243.
KEGG	ath:AT3G10740.
Organism-specific databases
TAIR	At3g10740.
Phylogenomic databases
eggNOG	COG3534.
HOGENOM	HOG000115340.
InParanoid	Q9SG80.
KO	K01209.
OMA	LEYLGIG.
PhylomeDB	Q9SG80.
ProtClustDB	CLSN2684036.
Gene expression databases
Genevestigator	Q9SG80.
Family and domain databases
InterPro	IPR010720. Alpha-L-AF_C. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF06964. Alpha-L-AF_C. 1 hit. [Graphical view]
SMART	SM00813. Alpha-L-AF_C. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASD1_ARATH

Accession

Primary (citable) accession number: Q9SG80
Secondary accession number(s): Q56W72

Q94JT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents