Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9SG80

- ASD1_ARATH

UniProt

Q9SG80 - ASD1_ARATH

Protein

Alpha-L-arabinofuranosidase 1

Gene

ASD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May be involved in the coordinated dissolution of the cell wall matrix during abscission and in the secondary cell wall formation in xylem vessels. Prefers arabinoxylan, but may also use pectic arabinans as substrates.3 Publications

    Catalytic activityi

    Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.2 Publications

    Kineticsi

    1. KM=0.48 mM for p-nitrophenyl-beta-D-xylopyranoside2 Publications
    2. KM=5.2 mM for (1,5)-alpha-L-arabinobiose (at 37 degrees Celsius)2 Publications

    pH dependencei

    Optimum pH is 4.3.2 Publications

    Temperature dependencei

    Optimum temperature is 67 degrees Celsius.2 Publications

    GO - Molecular functioni

    1. alpha-L-arabinofuranosidase activity Source: TAIR
    2. xylan 1,4-beta-xylosidase activity Source: TAIR

    GO - Biological processi

    1. L-arabinose metabolic process Source: InterPro
    2. xylan catabolic process Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT3G10740-MONOMER.
    SABIO-RKQ9SG80.

    Protein family/group databases

    CAZyiGH51. Glycoside Hydrolase Family 51.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-L-arabinofuranosidase 1 (EC:3.2.1.55)
    Short name:
    AtASD1
    Alternative name(s):
    Beta-D-xylosidase (EC:3.2.1.-)
    Gene namesi
    Name:ASD1
    Synonyms:ARAF, ARAF1
    Ordered Locus Names:At3g10740
    ORF Names:T7M13.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G10740.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. plant-type cell wall Source: TAIR
    3. proteinaceous extracellular matrix Source: UniProtKB-SubCell
    4. vacuole Source: TAIR

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype; even in asd1 and asd2 double mutant.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 678645Alpha-L-arabinofuranosidase 1PRO_0000384371Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi555 – 5551N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9SG80.
    PRIDEiQ9SG80.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, flowers, stems, siliques and seedlings. Observed in zones of cell proliferation, the vascular system and floral abscission zones. Expressed in the guard cells in stems, in xylem vessels and parenchyma cells surrounding the vessels, in the cambium and in the phloem, but not in the secondary xylem.3 Publications

    Inductioni

    Not induced by hormones or during leaf senescence.1 Publication

    Gene expression databases

    GenevestigatoriQ9SG80.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G10740.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SG80.
    SMRiQ9SG80. Positions 229-559.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini152 – 23988CBM-cenCAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 51 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3534.
    HOGENOMiHOG000115340.
    InParanoidiQ9SG80.
    KOiK01209.
    OMAiCITHGLG.
    PhylomeDBiQ9SG80.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    InterProiIPR010720. Alpha-L-AF_C.
    IPR008979. Galactose-bd-like.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF06964. Alpha-L-AF_C. 1 hit.
    [Graphical view]
    SMARTiSM00813. Alpha-L-AF_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SG80-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDMESWKLLR SVCVLSFLLG SCFVYQSLRV VDAQEDPKPA VTLQVDASNG    50
    GGRPIPETLF GIFFEEINHA GAGGLWAELV SNRGFEAGGQ NTPSNIWPWS 100
    IVGDHSSIYV ATDRSSCFER NKIALRMDVL CDSKGCPSGG VGVYNPGYWG 150
    MNIEEGKKYK VALYVRSTGD IDLSVSLTSS NGSRTLASEK IIASASDVSK 200
    WIKKEVLLEA KATDPSARLQ LTTTKKGSIW IDQVSAMPVD THKGHGFRND 250
    LFQMMADIKP RFIRFPGGCF VEGEWLSNAF RWKETVGPWE ERPGHFGDVW 300
    KYWTDDGLGH FEFFQMAEDI GAAPIWVFNN GISHNDEVET ASIMPFVQEA 350
    LDGIEFARGD ANSTWGSVRA KMGRQEPFEL KYVAIGNEDC GKTYYRGNYI 400
    VFYDAIKKAY PDIKIISNCD GSSHPLDHPA DYYDYHIYTS ASNLFSMYHQ 450
    FDRTSRKGPK AFVSEYAVTG KDAGTGSLLA SLAEAAFLIG LEKNSDIVEM 500
    ASYAPLFVNT NDRRWNPDAI VFNSSHLYGT PSYWVQRFFA ESSGATLLTS 550
    TLKGNSTSLV ASAISWKNNG KDYIRIKAVN FGANSENMQV LVTGLDPNVM 600
    RVSGSKKTVL TSTNVMDENS FSQPEKVVPH ESLLELAEED MTVVLPPHSF 650
    SSFDLLKESA KIRMPISDSS SHQKTTTV 678
    Length:678
    Mass (Da):75,045
    Last modified:May 1, 2000 - v1
    Checksum:i8116546072E6CA9A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti251 – 2511L → P in AAN28883. (PubMed:14593172)Curated
    Sequence conflicti251 – 2511L → P in AAK50089. (PubMed:14593172)Curated
    Sequence conflicti253 – 2542QM → HL in AAO92261. (PubMed:14512381)Curated
    Sequence conflicti453 – 4531R → G in AAO92261. (PubMed:14512381)Curated
    Sequence conflicti499 – 4991E → D in AAO92261. (PubMed:14512381)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY243509 mRNA. Translation: AAO92261.1.
    AC011708 Genomic DNA. Translation: AAF19575.1.
    CP002686 Genomic DNA. Translation: AEE74949.1.
    AF372949 mRNA. Translation: AAK50089.1.
    AY143944 mRNA. Translation: AAN28883.1.
    AK222175 mRNA. Translation: BAD95302.1.
    AK220766 mRNA. Translation: BAD93969.1.
    RefSeqiNP_187685.1. NM_111911.4.
    UniGeneiAt.20271.

    Genome annotation databases

    EnsemblPlantsiAT3G10740.1; AT3G10740.1; AT3G10740.
    GeneIDi820243.
    KEGGiath:AT3G10740.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY243509 mRNA. Translation: AAO92261.1 .
    AC011708 Genomic DNA. Translation: AAF19575.1 .
    CP002686 Genomic DNA. Translation: AEE74949.1 .
    AF372949 mRNA. Translation: AAK50089.1 .
    AY143944 mRNA. Translation: AAN28883.1 .
    AK222175 mRNA. Translation: BAD95302.1 .
    AK220766 mRNA. Translation: BAD93969.1 .
    RefSeqi NP_187685.1. NM_111911.4.
    UniGenei At.20271.

    3D structure databases

    ProteinModelPortali Q9SG80.
    SMRi Q9SG80. Positions 229-559.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G10740.1-P.

    Protein family/group databases

    CAZyi GH51. Glycoside Hydrolase Family 51.

    Proteomic databases

    PaxDbi Q9SG80.
    PRIDEi Q9SG80.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G10740.1 ; AT3G10740.1 ; AT3G10740 .
    GeneIDi 820243.
    KEGGi ath:AT3G10740.

    Organism-specific databases

    TAIRi AT3G10740.

    Phylogenomic databases

    eggNOGi COG3534.
    HOGENOMi HOG000115340.
    InParanoidi Q9SG80.
    KOi K01209.
    OMAi CITHGLG.
    PhylomeDBi Q9SG80.

    Enzyme and pathway databases

    BioCyci ARA:AT3G10740-MONOMER.
    SABIO-RK Q9SG80.

    Gene expression databases

    Genevestigatori Q9SG80.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    InterProi IPR010720. Alpha-L-AF_C.
    IPR008979. Galactose-bd-like.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF06964. Alpha-L-AF_C. 1 hit.
    [Graphical view ]
    SMARTi SM00813. Alpha-L-AF_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Two alpha-L-arabinofuranosidase genes in Arabidopsis thaliana are differentially expressed during vegetative growth and flower development."
      Fulton L.M., Cobbett C.S.
      J. Exp. Bot. 54:2467-2477(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY HORMONES.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 287-678.
      Strain: cv. Columbia.
    6. "Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis."
      Minic Z., Rihouey C., Do C.T., Lerouge P., Jouanin L.
      Plant Physiol. 135:867-878(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    7. "Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis."
      Minic Z., Do C.-T., Rihouey C., Morin H., Lerouge P., Jouanin L.
      J. Exp. Bot. 57:2339-2351(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    8. "Cell wall modifications in Arabidopsis plants with altered alpha-L-arabinofuranosidase activity."
      Chavez Montes R.A., Ranocha P., Martinez Y., Minic Z., Jouanin L., Marquis M., Saulnier L., Fulton L.M., Cobbett C.S., Bitton F., Renou J.-P., Jauneau A., Goffner D.
      Plant Physiol. 147:63-77(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiASD1_ARATH
    AccessioniPrimary (citable) accession number: Q9SG80
    Secondary accession number(s): Q56W72
    , Q570E2, Q84RC5, Q94JT7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3