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Protein

Protein SAD1/UNC-84 domain protein 2

Gene

SUN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments (PubMed:24667841). Required for the maintenance and/or formation of polarized nuclear shape in root hairs (PubMed:21294795). Modulates the anchoring and mobility of WIP proteins in the nuclear envelope (NE) (PubMed:22270916).By similarity3 Publications

GO - Molecular functioni

  • protein anchor Source: UniProtKB

GO - Biological processi

  • nucleus organization Source: UniProtKB
  • protein heterooligomerization Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
  • protein localization to nuclear envelope Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SAD1/UNC-84 domain protein 21 Publication
Short name:
AtSUN21 Publication
Gene namesi
Name:SUN21 Publication
Ordered Locus Names:At3g10730Imported
ORF Names:T7M13.19Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G10730.

Subcellular locationi

  • Nucleus inner membrane 3 Publications; Single-pass type II membrane protein Sequence analysis
  • Cytoplasmcytoskeletonphragmoplast 1 Publication
  • Endoplasmic reticulum membrane 1 Publication; Single-pass type II membrane protein Sequence analysis
  • Nucleus envelope 1 Publication

  • Note: Dynamic localization during mitosis, tightly coupled with nuclear envelope (NE) dynamics. NE re-formation during metaphase is temporally and spatially coordinated with plant-specific microtubule structures such as phragmoplasts. During anaphase, after NE breakdown (NEBD), predominantly localized with the endoplasmic reticulum, in the outside of the segregated chromosomes and not in between segregated chromosomes.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 105104NuclearCuratedAdd
BLAST
Transmembranei106 – 12823HelicalSequence analysisAdd
BLAST
Topological domaini129 – 455327Perinuclear spaceCuratedAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • nuclear envelope Source: UniProtKB
  • nuclear inner membrane Source: UniProtKB-SubCell
  • phragmoplast Source: UniProtKB
  • spindle Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. When associated with SUN1 disruption, abnormal nuclear shape in some cells such as mature root hairs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 455454Protein SAD1/UNC-84 domain protein 2PRO_0000432817Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei63 – 631PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9SG79.

PTM databases

iPTMnetiQ9SG79.

Expressioni

Tissue specificityi

Expressed in roots, hypocotyls, cotyledons and leaves and inflorescences.2 Publications

Inductioni

Up-regulated in proliferating tissues.1 Publication

Gene expression databases

GenevisibleiQ9SG79. AT.

Interactioni

Subunit structurei

Forms homomers (e.g. dimers, trimers and tetramers) and heteromers with SUN1 (PubMed:19807882). Core component of the LINC complex which is composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane WIP proteins, the nucleoskeletal CRWN/LINC proteins, and, possibly, KAKU4. Interacts with LINC1, WIP1, WIP2 and WIP3 at the nuclear envelope (NE) (PubMed:22270916, PubMed:24667841).3 Publications

GO - Molecular functioni

  • protein anchor Source: UniProtKB

Protein-protein interaction databases

IntActiQ9SG79. 1 interaction.
STRINGi3702.AT3G10730.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SG79.
SMRiQ9SG79. Positions 262-444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini285 – 447163SUNPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili201 – 22525Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi88 – 958Nuclear localization signalPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi78 – 8710Poly-ThrSequence analysis

Domaini

The SUN domain may play a role in the nuclear anchoring and/or migration (By similarity). The SUN domain is required for interactions with WIP proteins (PubMed:22270916).By similarity1 Publication

Sequence similaritiesi

Contains 1 SUN domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2687. Eukaryota.
ENOG410YM6S. LUCA.
HOGENOMiHOG000237750.
KOiK19347.
OMAiVKHSEPF.
OrthoDBiEOG093609UX.
PhylomeDBiQ9SG79.

Family and domain databases

InterProiIPR012919. SUN_dom.
[Graphical view]
PfamiPF07738. Sad1_UNC. 1 hit.
[Graphical view]
PROSITEiPS51469. SUN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SG79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASTVSITA SPRTIRRTPV LSGEKKSNFD FPPSESHANA AIGESSAGTN
60 70 80 90 100
KDLIRAEAAG ERSNTYDVGP VTRKSGSTAT GTNTTTTQRR TRKSQGNKID
110 120 130 140 150
RGKWKTVVRV FAKQFGALLL LVGLIQLIRK LTLKDSSLSS SNFPIETEMV
160 170 180 190 200
LSELESRISA VDGLVKTTTK MMQVQVEFLD KKMDSESRAL RQTIDSTSSV
210 220 230 240 250
LHSELKKVES KTERLQVSVD ELNAKPLVSR EELERVYEEL KKGKVGDSDV
260 270 280 290 300
NIDKLRAYAR DIVEKEIGKH VADGLGRVDY ALASGGAFVM GHSDPFLVGN
310 320 330 340 350
GRNWFGTSRR RVHSKAVKML TPSFGEPGQC FPLKGSNGYV LVRLRAPIIP
360 370 380 390 400
EAVTLEHVSK AVAYDRSSAP KDCRVSGWLG DIDMETETMP LLTEFSYDLD
410 420 430 440 450
RSNAQTFDIA DSAHSGLVNT VRLDFNSNHG SSSHTCIYRF RVHGRELDSV

SVAHA
Length:455
Mass (Da):49,940
Last modified:May 1, 2000 - v1
Checksum:iBB99EFF2B3EBF917
GO

Sequence cautioni

The sequence AAM65947 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021R → G in AAM65947 (Ref. 5) Curated
Sequence conflicti398 – 3981D → N in AAM65947 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011708 Genomic DNA. Translation: AAF19576.1.
CP002686 Genomic DNA. Translation: AEE74948.1.
AK118080 mRNA. Translation: BAC42710.1.
BT006045 mRNA. Translation: AAP04030.1.
AY088410 mRNA. Translation: AAM65947.1. Different initiation.
RefSeqiNP_566380.2. NM_111910.3.
UniGeneiAt.43435.

Genome annotation databases

EnsemblPlantsiAT3G10730.1; AT3G10730.1; AT3G10730.
GeneIDi820242.
GrameneiAT3G10730.1; AT3G10730.1; AT3G10730.
KEGGiath:AT3G10730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011708 Genomic DNA. Translation: AAF19576.1.
CP002686 Genomic DNA. Translation: AEE74948.1.
AK118080 mRNA. Translation: BAC42710.1.
BT006045 mRNA. Translation: AAP04030.1.
AY088410 mRNA. Translation: AAM65947.1. Different initiation.
RefSeqiNP_566380.2. NM_111910.3.
UniGeneiAt.43435.

3D structure databases

ProteinModelPortaliQ9SG79.
SMRiQ9SG79. Positions 262-444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9SG79. 1 interaction.
STRINGi3702.AT3G10730.1.

PTM databases

iPTMnetiQ9SG79.

Proteomic databases

PaxDbiQ9SG79.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G10730.1; AT3G10730.1; AT3G10730.
GeneIDi820242.
GrameneiAT3G10730.1; AT3G10730.1; AT3G10730.
KEGGiath:AT3G10730.

Organism-specific databases

TAIRiAT3G10730.

Phylogenomic databases

eggNOGiKOG2687. Eukaryota.
ENOG410YM6S. LUCA.
HOGENOMiHOG000237750.
KOiK19347.
OMAiVKHSEPF.
OrthoDBiEOG093609UX.
PhylomeDBiQ9SG79.

Miscellaneous databases

PROiQ9SG79.

Gene expression databases

GenevisibleiQ9SG79. AT.

Family and domain databases

InterProiIPR012919. SUN_dom.
[Graphical view]
PfamiPF07738. Sad1_UNC. 1 hit.
[Graphical view]
PROSITEiPS51469. SUN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSUN2_ARATH
AccessioniPrimary (citable) accession number: Q9SG79
Secondary accession number(s): Q8L9I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2015
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.