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Protein

CDPK-related kinase 6

Gene

CRK6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium and calmodulin. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei174ATPPROSITE-ProRule annotation1
Active sitei270Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi148 – 156ATPPROSITE-ProRule annotation9
Calcium bindingi458 – 4681By similarityAdd BLAST11
Calcium bindingi494 – 5052By similarityAdd BLAST12
Calcium bindingi534 – 5453By similarityAdd BLAST12
Calcium bindingi564 – 5754By similarityAdd BLAST12

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CDPK-related kinase 6 (EC:2.7.11.1)
Short name:
AtCRK6
Alternative name(s):
Calcium/calmodulin-dependent protein kinase CRK6
Gene namesi
Name:CRK6
Ordered Locus Names:At3g49370
ORF Names:F2K15.230, T1G12.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G49370.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00004205332 – 594CDPK-related kinase 6Add BLAST593

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei352Phosphoserine; by CPK1, CPK10 and CPK341 Publication1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ9SG12.

PTM databases

iPTMnetiQ9SG12.

Expressioni

Gene expression databases

GenevisibleiQ9SG12. AT.

Interactioni

Subunit structurei

Binds calmodulin (CaM) in a calcium-dependent manner.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G49370.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SG12.
SMRiQ9SG12.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini142 – 404Protein kinasePROSITE-ProRule annotationAdd BLAST263
Domaini445 – 480EF-hand 1Add BLAST36
Domaini481 – 516EF-hand 2Add BLAST36
Domaini517 – 556EF-hand 3Add BLAST40
Domaini557 – 586EF-hand 4Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni408 – 438Autoinhibitory domainBy similarityAdd BLAST31
Regioni427 – 447Calmodulin binding (CaMBD)By similarityAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi43 – 112Pro-richAdd BLAST70

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (408-438) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
KOiK00924.
OMAiQEMNLGP.
OrthoDBiEOG093606GB.
PhylomeDBiQ9SG12.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SG12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHCYSRNIS TVDDDDEIPS ATAQLPHRSH QNHHQTSSSS SIPQSPATSE
60 70 80 90 100
VNPYNISPFQ SPLPAGVAPS PARTPGRKFK WPFPPPSPAK PIMAALRRRR
110 120 130 140 150
GTAPHPRDGP IPEDSEAGGS GGGIGERLDK NFGFAKNFEG KYELGREVGR
160 170 180 190 200
GHFGHTCWAK AKKGKIKGQT VAVKIISKSK MTSALSIEDV RREVKLLKAL
210 220 230 240 250
SGHSHMVKFY DVFEDSDNVF VVMELCEGGE LLDSILARGG RYPEAEAKRI
260 270 280 290 300
LVQILSATAF FHLQGVVHRD LKPENFLFTS KNEDAVLKVI DFGLSDYARF
310 320 330 340 350
DQRLNDVVGS AYYVAPEVLH RSYSTEADIW SIGVISYILL CGSRPFYGRT
360 370 380 390 400
ESAIFRCVLR ANPNFDDLPW PSISPIAKDF VKRLLNKDHR KRMTAAQALA
410 420 430 440 450
HPWLRDENPG LLLDFSIYKL VKSYIRASPF RRAALKSLSK AIPEEELVFL
460 470 480 490 500
KAQFMLLEPE DGGLHLHNFT TALTRYATDA MIESRLPDIL NMMQPLAHKK
510 520 530 540 550
LDFEEFCAAS VSVYQLEALE EWEQIATVAF EHFESEGSRA ISVQELAEEM
560 570 580 590
SLGPNAYPLL KDWIRSLDGK LNFLGYAKFL HGVTVRSSSS RPMR
Length:594
Mass (Da):66,372
Last modified:May 1, 2000 - v1
Checksum:i2B170246FB708FB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012329 Genomic DNA. Translation: AAG52176.1.
AL132956 Genomic DNA. Translation: CAB66416.1.
CP002686 Genomic DNA. Translation: AEE78533.1.
PIRiT45842.
RefSeqiNP_190506.1. NM_114797.3.
UniGeneiAt.35614.

Genome annotation databases

EnsemblPlantsiAT3G49370.1; AT3G49370.1; AT3G49370.
GeneIDi824099.
GrameneiAT3G49370.1; AT3G49370.1; AT3G49370.
KEGGiath:AT3G49370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012329 Genomic DNA. Translation: AAG52176.1.
AL132956 Genomic DNA. Translation: CAB66416.1.
CP002686 Genomic DNA. Translation: AEE78533.1.
PIRiT45842.
RefSeqiNP_190506.1. NM_114797.3.
UniGeneiAt.35614.

3D structure databases

ProteinModelPortaliQ9SG12.
SMRiQ9SG12.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G49370.1.

PTM databases

iPTMnetiQ9SG12.

Proteomic databases

PaxDbiQ9SG12.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G49370.1; AT3G49370.1; AT3G49370.
GeneIDi824099.
GrameneiAT3G49370.1; AT3G49370.1; AT3G49370.
KEGGiath:AT3G49370.

Organism-specific databases

TAIRiAT3G49370.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
KOiK00924.
OMAiQEMNLGP.
OrthoDBiEOG093606GB.
PhylomeDBiQ9SG12.

Miscellaneous databases

PROiQ9SG12.

Gene expression databases

GenevisibleiQ9SG12. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAMK6_ARATH
AccessioniPrimary (citable) accession number: Q9SG12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.