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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A

Gene

OST1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT1G76400-MONOMER.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A (EC:2.4.99.18)
Alternative name(s):
Ribophorin IA
Short name:
RPN-IA
Ribophorin-1A
Gene namesi
Name:OST1A
Synonyms:RPN1A
Ordered Locus Names:At1g76400
ORF Names:F15M4.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G76400.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 432407LumenalSequence analysisAdd
BLAST
Transmembranei433 – 45321HelicalSequence analysisAdd
BLAST
Topological domaini454 – 614161CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 614589Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1APRO_0000420806Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi94 – 941N-linked (GlcNAc...)Sequence analysis
Glycosylationi299 – 2991N-linked (GlcNAc...)Sequence analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SFX3.
PRIDEiQ9SFX3.

PTM databases

iPTMnetiQ9SFX3.

Expressioni

Gene expression databases

GenevisibleiQ9SFX3. AT.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex.By similarity

Protein-protein interaction databases

BioGridi29191. 2 interactions.
STRINGi3702.AT1G76400.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SFX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the OST1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2291. Eukaryota.
ENOG410XQVZ. LUCA.
HOGENOMiHOG000247000.
InParanoidiQ9SFX3.
KOiK12666.
OMAiLLIEPRF.
PhylomeDBiQ9SFX3.

Family and domain databases

InterProiIPR007676. Ribophorin_I.
[Graphical view]
PANTHERiPTHR21049. PTHR21049. 1 hit.
PfamiPF04597. Ribophorin_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SFX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQSSVVDLL LLLLAIALLA TPAFSDLVLS KVERRIDVTS QIARVTKTLK
60 70 80 90 100
VVNSGSESVS EFALTFPKFL GNNLAYLSVA PSEGKGKSKR TLVNLSVREA
110 120 130 140 150
DQKGLPDSIS VYSVALPKPL SKGDTLTLEV VAAFTNVLQP FPEKITQGEI
160 170 180 190 200
HLVMLQESAQ YLSPYAVESQ SLSIKLPNAR IESYTKFENT KLQGSELKYG
210 220 230 240 250
PYKNLQSYSY SPIVVHFESK AAFAVAEKLV REIEVSHWGN VQVTENYNVV
260 270 280 290 300
HRGAQLKGEF SRLDFQARPN PRGASAFRHL LARLPPRAHS IYYRDDIGNI
310 320 330 340 350
STSEMKSDSK KTELLIEPRF PLFGGWKTFF TIGYGLPLTD FLFASEGKRF
360 370 380 390 400
LNISFGSPIL DLVTEKLIVQ VVLPEGSKDI SVTTPFAVKQ SQEIKYSHLD
410 420 430 440 450
IAGRPVVVLE KNNVVPDHNQ HIQVYYKFSN INLLSEPLML ISGFFILFIT
460 470 480 490 500
CIIYTRADIS ISKSSPSYLA KLQWDEVLAT LQEVQSIVQK CLATHDKLEA
510 520 530 540 550
SLRDLSRTGD IQTCKAARKS TDSLLKDLSK ELKPLLGFLQ SFPSASHISP
560 570 580 590 600
KVEELVVKEK ELQEKLMAKH TTVVEGYEKK SSGRDIENRI ASQQQKIIAL
610
RQEIEDLLEF IDEI
Length:614
Mass (Da):68,642
Last modified:May 1, 2000 - v1
Checksum:i80078AEBFA62FB17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012394 Genomic DNA. Translation: AAF16661.1.
CP002684 Genomic DNA. Translation: AEE35835.1.
AY081297 mRNA. Translation: AAL91186.1.
AF083780 mRNA. Translation: AAN60338.1.
AY128779 mRNA. Translation: AAM91179.1.
AY139776 mRNA. Translation: AAM98094.1.
PIRiF96791.
RefSeqiNP_177766.1. NM_106289.4.
UniGeneiAt.16778.
At.66969.

Genome annotation databases

EnsemblPlantsiAT1G76400.1; AT1G76400.1; AT1G76400.
GeneIDi843972.
GrameneiAT1G76400.1; AT1G76400.1; AT1G76400.
KEGGiath:AT1G76400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012394 Genomic DNA. Translation: AAF16661.1.
CP002684 Genomic DNA. Translation: AEE35835.1.
AY081297 mRNA. Translation: AAL91186.1.
AF083780 mRNA. Translation: AAN60338.1.
AY128779 mRNA. Translation: AAM91179.1.
AY139776 mRNA. Translation: AAM98094.1.
PIRiF96791.
RefSeqiNP_177766.1. NM_106289.4.
UniGeneiAt.16778.
At.66969.

3D structure databases

ProteinModelPortaliQ9SFX3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29191. 2 interactions.
STRINGi3702.AT1G76400.1.

PTM databases

iPTMnetiQ9SFX3.

Proteomic databases

PaxDbiQ9SFX3.
PRIDEiQ9SFX3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G76400.1; AT1G76400.1; AT1G76400.
GeneIDi843972.
GrameneiAT1G76400.1; AT1G76400.1; AT1G76400.
KEGGiath:AT1G76400.

Organism-specific databases

TAIRiAT1G76400.

Phylogenomic databases

eggNOGiKOG2291. Eukaryota.
ENOG410XQVZ. LUCA.
HOGENOMiHOG000247000.
InParanoidiQ9SFX3.
KOiK12666.
OMAiLLIEPRF.
PhylomeDBiQ9SFX3.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:AT1G76400-MONOMER.

Miscellaneous databases

PROiQ9SFX3.

Gene expression databases

GenevisibleiQ9SFX3. AT.

Family and domain databases

InterProiIPR007676. Ribophorin_I.
[Graphical view]
PANTHERiPTHR21049. PTHR21049. 1 hit.
PfamiPF04597. Ribophorin_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiOST1A_ARATH
AccessioniPrimary (citable) accession number: Q9SFX3
Secondary accession number(s): Q8H795
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.