Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A

Gene

OST1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein N-linked glycosylation Source: TAIR
  • protein N-linked glycosylation via asparagine Source: GO_Central

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A (EC:2.4.99.18)
Alternative name(s):
Ribophorin IA
Short name:
RPN-IA
Ribophorin-1A
Gene namesi
Name:OST1A
Synonyms:RPN1A
Ordered Locus Names:At1g76400
ORF Names:F15M4.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G76400
TAIRilocus:2014579 AT1G76400

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 432LumenalSequence analysisAdd BLAST407
Transmembranei433 – 453HelicalSequence analysisAdd BLAST21
Topological domaini454 – 614CytoplasmicSequence analysisAdd BLAST161

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000042080626 – 614Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1AAdd BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi299N-linked (GlcNAc...) asparagineSequence analysis1
Cross-linki311Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Glycosylationi352N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9SFX3
PRIDEiQ9SFX3

PTM databases

iPTMnetiQ9SFX3

Expressioni

Gene expression databases

ExpressionAtlasiQ9SFX3 baseline and differential
GenevisibleiQ9SFX3 AT

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex.By similarity

Protein-protein interaction databases

BioGridi29191, 2 interactors
STRINGi3702.AT1G76400.1

Structurei

3D structure databases

ProteinModelPortaliQ9SFX3
SMRiQ9SFX3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the OST1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2291 Eukaryota
ENOG410XQVZ LUCA
HOGENOMiHOG000247000
InParanoidiQ9SFX3
KOiK12666
OMAiRYPIFGG
OrthoDBiEOG0936060U
PhylomeDBiQ9SFX3

Family and domain databases

InterProiView protein in InterPro
IPR007676 Ribophorin_I
PANTHERiPTHR21049 PTHR21049, 1 hit
PfamiView protein in Pfam
PF04597 Ribophorin_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SFX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQSSVVDLL LLLLAIALLA TPAFSDLVLS KVERRIDVTS QIARVTKTLK
60 70 80 90 100
VVNSGSESVS EFALTFPKFL GNNLAYLSVA PSEGKGKSKR TLVNLSVREA
110 120 130 140 150
DQKGLPDSIS VYSVALPKPL SKGDTLTLEV VAAFTNVLQP FPEKITQGEI
160 170 180 190 200
HLVMLQESAQ YLSPYAVESQ SLSIKLPNAR IESYTKFENT KLQGSELKYG
210 220 230 240 250
PYKNLQSYSY SPIVVHFESK AAFAVAEKLV REIEVSHWGN VQVTENYNVV
260 270 280 290 300
HRGAQLKGEF SRLDFQARPN PRGASAFRHL LARLPPRAHS IYYRDDIGNI
310 320 330 340 350
STSEMKSDSK KTELLIEPRF PLFGGWKTFF TIGYGLPLTD FLFASEGKRF
360 370 380 390 400
LNISFGSPIL DLVTEKLIVQ VVLPEGSKDI SVTTPFAVKQ SQEIKYSHLD
410 420 430 440 450
IAGRPVVVLE KNNVVPDHNQ HIQVYYKFSN INLLSEPLML ISGFFILFIT
460 470 480 490 500
CIIYTRADIS ISKSSPSYLA KLQWDEVLAT LQEVQSIVQK CLATHDKLEA
510 520 530 540 550
SLRDLSRTGD IQTCKAARKS TDSLLKDLSK ELKPLLGFLQ SFPSASHISP
560 570 580 590 600
KVEELVVKEK ELQEKLMAKH TTVVEGYEKK SSGRDIENRI ASQQQKIIAL
610
RQEIEDLLEF IDEI
Length:614
Mass (Da):68,642
Last modified:May 1, 2000 - v1
Checksum:i80078AEBFA62FB17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012394 Genomic DNA Translation: AAF16661.1
CP002684 Genomic DNA Translation: AEE35835.1
AY081297 mRNA Translation: AAL91186.1
AF083780 mRNA Translation: AAN60338.1
AY128779 mRNA Translation: AAM91179.1
AY139776 mRNA Translation: AAM98094.1
PIRiF96791
RefSeqiNP_177766.1, NM_106289.4
UniGeneiAt.16778
At.66969

Genome annotation databases

EnsemblPlantsiAT1G76400.1; AT1G76400.1; AT1G76400
GeneIDi843972
GrameneiAT1G76400.1; AT1G76400.1; AT1G76400
KEGGiath:AT1G76400

Similar proteinsi

Entry informationi

Entry nameiOST1A_ARATH
AccessioniPrimary (citable) accession number: Q9SFX3
Secondary accession number(s): Q8H795
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: May 1, 2000
Last modified: June 20, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health