Q9SFU6 (CALS9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Callose synthase 9
EC=2.4.1.34

Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 10

Gene names

Name:	CALS9
Synonyms:	GSL10
Ordered Locus Names:	At3g07160
ORF Names:	T1B9.18

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1931 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in sporophytic and gametophytic development. Required for normal plant development. During pollen formation, required for the entry of microspores into mitosis and microspore symmetric division. May be required for correct temporal and spatial control of callose deposition during pollen mitosis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals. Ref.5
Catalytic activity	UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Developmental stage	Expressed throughout pollen development with a peak at bicellular pollen stage. Ref.5
Disruption phenotype	Plants develop deformed or collapsed and inviable pollen grains. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 48 family.
Sequence caution	The sequence AAM61660.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	(1->3)-beta-D-glucan biosynthetic process Inferred from electronic annotation. Source: InterPro callose deposition in cell wall Inferred from mutant phenotype Ref.5. Source: TAIR cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW developmental growth Inferred from mutant phenotype Ref.5. Source: TAIR generative cell mitosis Inferred from mutant phenotype. Source: TAIR microsporogenesis Inferred from mutant phenotype Ref.5. Source: TAIR pollen germination Inferred from mutant phenotype. Source: TAIR regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW regulation of pollen tube growth Inferred from mutant phenotype. Source: TAIR
Cellular component	1,3-beta-D-glucan synthase complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,3-beta-D-glucan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1931	1931	Callose synthase 9		PRO_0000334581
Regions
	Topological domain	1 – 489	489	Cytoplasmic Potential
	Transmembrane	490 – 510	21	Helical; Potential
	Topological domain	511 – 523	13	Extracellular Potential
	Transmembrane	524 – 544	21	Helical; Potential
	Topological domain	545 – 560	16	Cytoplasmic Potential
	Transmembrane	561 – 581	21	Helical; Potential
	Topological domain	582 – 591	10	Extracellular Potential
	Transmembrane	592 – 612	21	Helical; Potential
	Topological domain	613 – 681	69	Cytoplasmic Potential
	Transmembrane	682 – 702	21	Helical; Potential
	Topological domain	703 – 747	45	Extracellular Potential
	Transmembrane	748 – 768	21	Helical; Potential
	Topological domain	769 – 1498	730	Cytoplasmic Potential
	Transmembrane	1499 – 1519	21	Helical; Potential
	Topological domain	1520 – 1553	34	Extracellular Potential
	Transmembrane	1554 – 1574	21	Helical; Potential
	Topological domain	1575 – 1580	6	Cytoplasmic Potential
	Transmembrane	1581 – 1601	21	Helical; Potential
	Topological domain	1602 – 1650	49	Extracellular Potential
	Transmembrane	1651 – 1671	21	Helical; Potential
	Transmembrane	1672 – 1692	21	Helical; Potential
	Topological domain	1693 – 1744	52	Extracellular Potential
	Transmembrane	1745 – 1765	21	Helical; Potential
	Topological domain	1766 – 1773	8	Cytoplasmic Potential
	Transmembrane	1774 – 1794	21	Helical; Potential
	Topological domain	1795 – 1809	15	Extracellular Potential
	Transmembrane	1810 – 1830	21	Helical; Potential
	Topological domain	1831 – 1836	6	Cytoplasmic Potential
	Transmembrane	1837 – 1857	21	Helical; Potential
	Topological domain	1858 – 1879	22	Extracellular Potential
	Transmembrane	1880 – 1900	21	Helical; Potential
	Topological domain	1901 – 1931	31	Cytoplasmic Potential
Experimental info
	Sequence conflict	1618	1	R → M in AAM61660. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9SFU6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: BBC1F8649943ABCA	FASTA	1,931	222,091
	10 20 30 40 50 60 MSRAESSWER LVNAALRRDR TGGVAGGNQS SIVGYVPSSL SNNRDIDAIL RAADEIQDED 70 80 90 100 110 120 PNIARILCEH GYSLAQNLDP NSEGRGVLQF KTGLMSVIKQ KLAKREVGTI DRSQDILRLQ 130 140 150 160 170 180 EFYRLYREKN NVDTLKEEEK QLRESGAFTD ELERKTVKRK RVFATLKVLG SVLEQLAKEI 190 200 210 220 230 240 PEELKHVIDS DAAMSEDTIA YNIIPLDAPV TTNATTTFPE VQAAVAALKY FPGLPKLPPD 250 260 270 280 290 300 FPIPATRTAD MLDFLHYIFG FQKDSVSNQR EHIVLLLANE QSRLNIPEET EPKLDDAAVR 310 320 330 340 350 360 KVFLKSLENY IKWCDYLCIQ PAWSNLEAIN GDKKLLFLSL YFLIWGEAAN IRFLPECLCY 370 380 390 400 410 420 IFHHMVREMD EILRQQVARP AESCMPVDSR GSDDGVSFLD HVIAPLYGVV SAEAFNNDNG 430 440 450 460 470 480 RAPHSAWRNY DDFNEYFWSL HSFELGWPWR TSSSFFQKPI PRKKLKTGRA KHRGKTSFVE 490 500 510 520 530 540 HRTFLHLYHS FHRLWIFLAM MFQALAIIAF NKDDLTSRKT LLQILSLGPT FVVMKFSESV 550 560 570 580 590 600 LEVIMMYGAY STTRRLAVSR IFLRFIWFGL ASVFISFLYV KSLKAPNSDS PIVQLYLIVI 610 620 630 640 650 660 AIYGGVQFFF SILMRIPTCH NIANKCDRWP VIRFFKWMRQ ERHYVGRGMY ERTSDFINLL 670 680 690 700 710 720 PINFNDYYTV VFLLVWEKQQ TYLYLLFWLV VLSAKFSFAY FLQIKPLVGP TRMIVKQNNI 730 740 750 760 770 780 PYSWHDFVSR KNYNALTVAS LWAPVVAIYL LDIHIFYTIF SAFLGFLLGA RDRLGEIRSL 790 800 810 820 830 840 EAIHKLFEEF PGAFMRALHV PLTNRQGDWH VISSHYCCSY LHVIINSKTV DKKNKVDAAH 850 860 870 880 890 900 FAPFWNQIIK SLREEDYITD FEMELLLMPK NSGRLELVQW PLFLLSSKIL LAKEIAAESN 910 920 930 940 950 960 SQEEILERIE RDDYMKYAVE EVYHTLKLVL TETLEAEGRL WVERIYEDIQ TSLKERNIHH 970 980 990 1000 1010 1020 DFQLNKLSLV ITRVTALLGI LKENETPEHA KGAIKALQDL YDVMRLDILT FNMRGHYETW 1030 1040 1050 1060 1070 1080 NLLTQAWNEG RLFTKLKWPK DPELKALVKR LYSLFTIKDS AAHVPRNLEA RRRLQFFTNS 1090 1100 1110 1120 1130 1140 LFMDVPPPKS VRKMLSFSVF TPYYSEVVLY SMAELTKRNE DGISILFYLQ KIYPDEWKNF 1150 1160 1170 1180 1190 1200 LARIGRDENA LEGDLDNERD ILELRFWASY RGQTLARTVR GMMYYRKALM LQSYLERKAG 1210 1220 1230 1240 1250 1260 NDATDAEGFE LSPEARAQAD LKFTYVVTCQ IYGRQKEDQK PEAVDIALLM QRNEALRIAY 1270 1280 1290 1300 1310 1320 IDVVDSPKEG KSHTEYYSKL VKADISGKDK EIYSIKLPGD PKLGEGKPEN QNHAIVFTRG 1330 1340 1350 1360 1370 1380 NAIQTIDMNQ DNYFEEALKM RNLLEEFDRD HGIRPPTILG VREHVFTGSV SSLASFMSNQ 1390 1400 1410 1420 1430 1440 ETSFVTLGQR VLAKPLKIRM HYGHPDVFDR VFHITRGGIS KASRVINISE DIFAGFNTTL 1450 1460 1470 1480 1490 1500 RQGNVTHHEY IQVGKGRDVG LNQIALFEGK VAGGNGEQVL SRDVYRLGQL LDFFRMMSFF 1510 1520 1530 1540 1550 1560 FTTVGFYLCT MLTVLTVYIF LYGRAYLALS GVGATIRERA ILLDDTALSA ALNAQFLFQI 1570 1580 1590 1600 1610 1620 GVFTAVPMVL GFILEQGFLQ AIVSFITMQF QLCTVFFTFS LGTRTHYFGR TILHGGARYQ 1630 1640 1650 1660 1670 1680 ATGRGFVVKH IKFSENYRLY SRSHFVKAME VILLLVVYLA YGNDEAGAVS YILLTVSSWF 1690 1700 1710 1720 1730 1740 LAVSWLFAPY LFNPAGFEWQ KVVEDFKEWT NWLFYRGGIG VKGAESWEAW WEEELSHIRT 1750 1760 1770 1780 1790 1800 LSGRIMETIL SLRFFIFQYG IVYKLKLQGS DTSFAVYGWS WVAFAMIIVL FKVFTFSQKI 1810 1820 1830 1840 1850 1860 SVNFQLLLRF IQGLSLLMAL AGIIVAVVLT PLSVTDIFAC VLAFIPTGWG ILSIACAWKP 1870 1880 1890 1900 1910 1920 VLKRMGMWKS IRSLARLYDA LMGMLIFLPV ALCSWFPFVS TFQTRMMFNQ AFSRGLEISL 1930 ILAGDNPNSG L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1573-1931.
[3]	"A cell plate-specific callose synthase and its interaction with phragmoplastin." Hong Z., Delauney A.J., Verma D.P.S. Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract] Cited for: GENE FAMILY AND NOMENCLATURE.
[4]	"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility." Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E. Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract] Cited for: NOMENCLATURE.
[5]	"Dual function of Arabidopsis glucan synthase-like genes GSL8 and GSL10 in male gametophyte development and plant growth." Toeller A., Brownfield L., Neu C., Twell D., Schulze-Lefert P. Plant J. 54:911-923(2008) [PubMed: 18315544] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC012395 Genomic DNA. Translation: AAF20230.1. AY085106 mRNA. Translation: AAM61660.1. Different initiation.
IPI	IPI01020150.
3D structure databases
ProteinModelPortal	Q9SFU6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SFU6.
Protein family/group databases
CAZy	GT48. Glycosyltransferase Family 48.
Proteomic databases
PRIDE	Q9SFU6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus AT3G07160 in contig BA000014_GR.
NMPDR	fig|3702.1.peg.12738.
Organism-specific databases
TAIR	At3g07160.
Phylogenomic databases
HOGENOM	HBG316390.
InParanoid	Q9SFU6.
OMA	DPNSEGR.
PhylomeDB	Q9SFU6.
Gene expression databases
Genevestigator	Q9SFU6.
Family and domain databases
InterPro	IPR003440. Glyco_trans_48. [Graphical view]
Pfam	PF02364. Glucan_synthase. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALS9_ARATH

Accession

Primary (citable) accession number: Q9SFU6
Secondary accession number(s): Q8LF10

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program