Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9SFU3

- PPA15_ARATH

UniProt

Q9SFU3 - PPA15_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Purple acid phosphatase 15

Gene

PAP15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acid phosphatase activity with p-nitrophenyl phosphate (pNPP), D-myoinositol 1-phosphate (Ins1P1), phytic acid and Myo-inositol hexakisphosphate. Low or no activity with Glc-6-P and ATP. Confers shoot growth stimulation, enhanced salt and osmotic stress tolerance, and ABA insensitivity. May modulate ascorbic acid (AsA) levels by controlling the input of myoinositol into this branch of AsA biosynthesis.1 Publication

Catalytic activityi

Myo-inositol hexakisphosphate + H2O = myo-inositol pentakisphosphate + phosphate.
A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

pH dependencei

Optimum pH is 4.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi194 – 1941IronBy similarity
Metal bindingi221 – 2211IronBy similarity
Metal bindingi221 – 2211ZincBy similarity
Metal bindingi224 – 2241IronBy similarity
Metal bindingi277 – 2771ZincBy similarity
Binding sitei277 – 2771SubstrateBy similarity
Metal bindingi359 – 3591ZincBy similarity
Active sitei369 – 3691Proton donorBy similarity
Metal bindingi396 – 3961ZincBy similarity
Metal bindingi398 – 3981IronBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. pollen germination Source: TAIR
  3. seed germination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G07130-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 15 (EC:3.1.3.-, EC:3.1.3.2)
Alternative name(s):
Phytase
Gene namesi
Name:PAP15
Synonyms:AT1
Ordered Locus Names:At3g07130
ORF Names:T1B9.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G07130.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 532513Purple acid phosphatase 15PRO_0000372819Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi264 – 2641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence Analysis
Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SFU3.
PRIDEiQ9SFU3.

Expressioni

Tissue specificityi

Expressed in roots, stems, cotyledons, leaves, flowers and siliques.2 Publications

Gene expression databases

GenevestigatoriQ9SFU3.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G07130.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SFU3.
SMRiQ9SFU3. Positions 102-518.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni396 – 3983Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238330.
InParanoidiQ9SFU3.
OMAiMHPLPGY.
PhylomeDBiQ9SFU3.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SFU3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTFLLLLLFC FLSPAISSAH SIPSTLDGPF VPVTVPLDTS LRGQAIDLPD
60 70 80 90 100
TDPRVRRRVI GFEPEQISLS LSSDHDSIWV SWITGEFQIG KKVKPLDPTS
110 120 130 140 150
INSVVQFGTL RHSLSHEAKG HSLVYSQLYP FDGLLNYTSG IIHHVRITGL
160 170 180 190 200
KPSTIYYYRC GDPSRRAMSK IHHFRTMPVS SPSSYPGRIA VVGDLGLTYN
210 220 230 240 250
TTDTISHLIH NSPDLILLIG DVSYANLYLT NGTSSDCYSC SFPETPIHET
260 270 280 290 300
YQPRWDYWGR FMENLTSKVP LMVIEGNHEI ELQAENKTFE AYSSRFAFPF
310 320 330 340 350
NESGSSSTLY YSFNAGGIHF VMLGAYIAYD KSAEQYEWLK KDLAKVDRSV
360 370 380 390 400
TPWLVASWHP PWYSSYTAHY REAECMKEAM EELLYSYGTD IVFNGHVHAY
410 420 430 440 450
ERSNRVYNYE LDPCGPVYIV IGDGGNREKM AIEHADDPGK CPEPLTTPDP
460 470 480 490 500
VMGGFCAWNF TPSDKFCWDR QPDYSALRES SFGHGILEMK NETWALWTWY
510 520 530
RNQDSSSEVG DQIYIVRQPD RCPLHHRLVN HC
Length:532
Mass (Da):60,435
Last modified:May 1, 2000 - v1
Checksum:i658F6C88779444CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448726 mRNA. Translation: AAN74650.1.
AC012395 Genomic DNA. Translation: AAF20233.1.
CP002686 Genomic DNA. Translation: AEE74502.1.
RefSeqiNP_187369.1. NM_111593.2.
UniGeneiAt.40395.
At.71829.

Genome annotation databases

EnsemblPlantsiAT3G07130.1; AT3G07130.1; AT3G07130.
GeneIDi819899.
KEGGiath:AT3G07130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448726 mRNA. Translation: AAN74650.1 .
AC012395 Genomic DNA. Translation: AAF20233.1 .
CP002686 Genomic DNA. Translation: AEE74502.1 .
RefSeqi NP_187369.1. NM_111593.2.
UniGenei At.40395.
At.71829.

3D structure databases

ProteinModelPortali Q9SFU3.
SMRi Q9SFU3. Positions 102-518.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G07130.1-P.

Proteomic databases

PaxDbi Q9SFU3.
PRIDEi Q9SFU3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G07130.1 ; AT3G07130.1 ; AT3G07130 .
GeneIDi 819899.
KEGGi ath:AT3G07130.

Organism-specific databases

TAIRi AT3G07130.

Phylogenomic databases

eggNOGi COG1409.
HOGENOMi HOG000238330.
InParanoidi Q9SFU3.
OMAi MHPLPGY.
PhylomeDBi Q9SFU3.

Enzyme and pathway databases

BioCyci ARA:AT3G07130-MONOMER.

Gene expression databases

Genevestigatori Q9SFU3.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "An Arabidopsis purple acid phosphatase with phytase activity increases foliar ascorbate."
    Zhang W., Gruszewski H.A., Chevone B.I., Nessler C.L.
    Plant Physiol. 146:431-440(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPPA15_ARATH
AccessioniPrimary (citable) accession number: Q9SFU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3