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Q9SFU3

- PPA15_ARATH

UniProt

Q9SFU3 - PPA15_ARATH

Protein

Purple acid phosphatase 15

Gene

PAP15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Acid phosphatase activity with p-nitrophenyl phosphate (pNPP), D-myoinositol 1-phosphate (Ins1P1), phytic acid and Myo-inositol hexakisphosphate. Low or no activity with Glc-6-P and ATP. Confers shoot growth stimulation, enhanced salt and osmotic stress tolerance, and ABA insensitivity. May modulate ascorbic acid (AsA) levels by controlling the input of myoinositol into this branch of AsA biosynthesis.1 Publication

    Catalytic activityi

    Myo-inositol hexakisphosphate + H2O = myo-inositol pentakisphosphate + phosphate.
    A phosphate monoester + H2O = an alcohol + phosphate.

    Cofactori

    Binds 1 iron ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    pH dependencei

    Optimum pH is 4.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi194 – 1941IronBy similarity
    Metal bindingi221 – 2211IronBy similarity
    Metal bindingi221 – 2211ZincBy similarity
    Metal bindingi224 – 2241IronBy similarity
    Metal bindingi277 – 2771ZincBy similarity
    Binding sitei277 – 2771SubstrateBy similarity
    Metal bindingi359 – 3591ZincBy similarity
    Active sitei369 – 3691Proton donorBy similarity
    Metal bindingi396 – 3961ZincBy similarity
    Metal bindingi398 – 3981IronBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: TAIR
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. pollen germination Source: TAIR
    3. seed germination Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT3G07130-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase 15 (EC:3.1.3.-, EC:3.1.3.2)
    Alternative name(s):
    Phytase
    Gene namesi
    Name:PAP15
    Synonyms:AT1
    Ordered Locus Names:At3g07130
    ORF Names:T1B9.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G07130.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 532513Purple acid phosphatase 15PRO_0000372819Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi264 – 2641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9SFU3.
    PRIDEiQ9SFU3.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, cotyledons, leaves, flowers and siliques.2 Publications

    Gene expression databases

    GenevestigatoriQ9SFU3.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT3G07130.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SFU3.
    SMRiQ9SFU3. Positions 44-518.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni396 – 3983Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1409.
    HOGENOMiHOG000238330.
    InParanoidiQ9SFU3.
    OMAiMHPLPGY.
    PhylomeDBiQ9SFU3.

    Family and domain databases

    Gene3Di2.60.40.380. 1 hit.
    3.60.21.10. 2 hits.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SFU3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTFLLLLLFC FLSPAISSAH SIPSTLDGPF VPVTVPLDTS LRGQAIDLPD    50
    TDPRVRRRVI GFEPEQISLS LSSDHDSIWV SWITGEFQIG KKVKPLDPTS 100
    INSVVQFGTL RHSLSHEAKG HSLVYSQLYP FDGLLNYTSG IIHHVRITGL 150
    KPSTIYYYRC GDPSRRAMSK IHHFRTMPVS SPSSYPGRIA VVGDLGLTYN 200
    TTDTISHLIH NSPDLILLIG DVSYANLYLT NGTSSDCYSC SFPETPIHET 250
    YQPRWDYWGR FMENLTSKVP LMVIEGNHEI ELQAENKTFE AYSSRFAFPF 300
    NESGSSSTLY YSFNAGGIHF VMLGAYIAYD KSAEQYEWLK KDLAKVDRSV 350
    TPWLVASWHP PWYSSYTAHY REAECMKEAM EELLYSYGTD IVFNGHVHAY 400
    ERSNRVYNYE LDPCGPVYIV IGDGGNREKM AIEHADDPGK CPEPLTTPDP 450
    VMGGFCAWNF TPSDKFCWDR QPDYSALRES SFGHGILEMK NETWALWTWY 500
    RNQDSSSEVG DQIYIVRQPD RCPLHHRLVN HC 532
    Length:532
    Mass (Da):60,435
    Last modified:May 1, 2000 - v1
    Checksum:i658F6C88779444CD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF448726 mRNA. Translation: AAN74650.1.
    AC012395 Genomic DNA. Translation: AAF20233.1.
    CP002686 Genomic DNA. Translation: AEE74502.1.
    RefSeqiNP_187369.1. NM_111593.2.
    UniGeneiAt.40395.
    At.71829.

    Genome annotation databases

    EnsemblPlantsiAT3G07130.1; AT3G07130.1; AT3G07130.
    GeneIDi819899.
    KEGGiath:AT3G07130.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF448726 mRNA. Translation: AAN74650.1 .
    AC012395 Genomic DNA. Translation: AAF20233.1 .
    CP002686 Genomic DNA. Translation: AEE74502.1 .
    RefSeqi NP_187369.1. NM_111593.2.
    UniGenei At.40395.
    At.71829.

    3D structure databases

    ProteinModelPortali Q9SFU3.
    SMRi Q9SFU3. Positions 44-518.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G07130.1-P.

    Proteomic databases

    PaxDbi Q9SFU3.
    PRIDEi Q9SFU3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G07130.1 ; AT3G07130.1 ; AT3G07130 .
    GeneIDi 819899.
    KEGGi ath:AT3G07130.

    Organism-specific databases

    TAIRi AT3G07130.

    Phylogenomic databases

    eggNOGi COG1409.
    HOGENOMi HOG000238330.
    InParanoidi Q9SFU3.
    OMAi MHPLPGY.
    PhylomeDBi Q9SFU3.

    Enzyme and pathway databases

    BioCyci ARA:AT3G07130-MONOMER.

    Gene expression databases

    Genevestigatori Q9SFU3.

    Family and domain databases

    Gene3Di 2.60.40.380. 1 hit.
    3.60.21.10. 2 hits.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
      Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
      Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
      Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
      J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "An Arabidopsis purple acid phosphatase with phytase activity increases foliar ascorbate."
      Zhang W., Gruszewski H.A., Chevone B.I., Nessler C.L.
      Plant Physiol. 146:431-440(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPPA15_ARATH
    AccessioniPrimary (citable) accession number: Q9SFU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3