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Protein

Purple acid phosphatase 15

Gene

PAP15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acid phosphatase activity with p-nitrophenyl phosphate (pNPP), D-myoinositol 1-phosphate (Ins1P1), phytic acid and Myo-inositol hexakisphosphate. Low or no activity with Glc-6-P and ATP. Confers shoot growth stimulation, enhanced salt and osmotic stress tolerance, and ABA insensitivity. May modulate ascorbic acid (AsA) levels by controlling the input of myoinositol into this branch of AsA biosynthesis.1 Publication

Catalytic activityi

Myo-inositol hexakisphosphate + H2O = myo-inositol pentakisphosphate + phosphate.
A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

pH dependencei

Optimum pH is 4.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi194IronBy similarity1
Metal bindingi221IronBy similarity1
Metal bindingi221ZincBy similarity1
Metal bindingi224IronBy similarity1
Metal bindingi277ZincBy similarity1
Binding sitei277SubstrateBy similarity1
Metal bindingi359ZincBy similarity1
Active sitei369Proton donorBy similarity1
Metal bindingi396ZincBy similarity1
Metal bindingi398IronBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • pollen germination Source: TAIR
  • seed germination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G07130-MONOMER.
BRENDAi3.1.3.2. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 15 (EC:3.1.3.-, EC:3.1.3.2)
Alternative name(s):
Phytase
Gene namesi
Name:PAP15
Synonyms:AT1
Ordered Locus Names:At3g07130
ORF Names:T1B9.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G07130.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000037281920 – 532Purple acid phosphatase 15Add BLAST513

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi136N-linked (GlcNAc...)Sequence analysis1
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Glycosylationi231N-linked (GlcNAc...)Sequence analysis1
Glycosylationi264N-linked (GlcNAc...)Sequence analysis1
Glycosylationi286N-linked (GlcNAc...)Sequence analysis1
Glycosylationi301N-linked (GlcNAc...)Sequence analysis1
Glycosylationi491N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SFU3.

Expressioni

Tissue specificityi

Expressed in roots, stems, cotyledons, leaves, flowers and siliques.2 Publications

Gene expression databases

GenevisibleiQ9SFU3. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G07130.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SFU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni396 – 398Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1378. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000238330.
InParanoidiQ9SFU3.
OMAiMHPLPGY.
OrthoDBiEOG093608JS.
PhylomeDBiQ9SFU3.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
PF16656. Pur_ac_phosph_N. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SFU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFLLLLLFC FLSPAISSAH SIPSTLDGPF VPVTVPLDTS LRGQAIDLPD
60 70 80 90 100
TDPRVRRRVI GFEPEQISLS LSSDHDSIWV SWITGEFQIG KKVKPLDPTS
110 120 130 140 150
INSVVQFGTL RHSLSHEAKG HSLVYSQLYP FDGLLNYTSG IIHHVRITGL
160 170 180 190 200
KPSTIYYYRC GDPSRRAMSK IHHFRTMPVS SPSSYPGRIA VVGDLGLTYN
210 220 230 240 250
TTDTISHLIH NSPDLILLIG DVSYANLYLT NGTSSDCYSC SFPETPIHET
260 270 280 290 300
YQPRWDYWGR FMENLTSKVP LMVIEGNHEI ELQAENKTFE AYSSRFAFPF
310 320 330 340 350
NESGSSSTLY YSFNAGGIHF VMLGAYIAYD KSAEQYEWLK KDLAKVDRSV
360 370 380 390 400
TPWLVASWHP PWYSSYTAHY REAECMKEAM EELLYSYGTD IVFNGHVHAY
410 420 430 440 450
ERSNRVYNYE LDPCGPVYIV IGDGGNREKM AIEHADDPGK CPEPLTTPDP
460 470 480 490 500
VMGGFCAWNF TPSDKFCWDR QPDYSALRES SFGHGILEMK NETWALWTWY
510 520 530
RNQDSSSEVG DQIYIVRQPD RCPLHHRLVN HC
Length:532
Mass (Da):60,435
Last modified:May 1, 2000 - v1
Checksum:i658F6C88779444CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448726 mRNA. Translation: AAN74650.1.
AC012395 Genomic DNA. Translation: AAF20233.1.
CP002686 Genomic DNA. Translation: AEE74502.1.
RefSeqiNP_187369.1. NM_111593.3.
UniGeneiAt.40395.
At.71829.

Genome annotation databases

EnsemblPlantsiAT3G07130.1; AT3G07130.1; AT3G07130.
GeneIDi819899.
GrameneiAT3G07130.1; AT3G07130.1; AT3G07130.
KEGGiath:AT3G07130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448726 mRNA. Translation: AAN74650.1.
AC012395 Genomic DNA. Translation: AAF20233.1.
CP002686 Genomic DNA. Translation: AEE74502.1.
RefSeqiNP_187369.1. NM_111593.3.
UniGeneiAt.40395.
At.71829.

3D structure databases

ProteinModelPortaliQ9SFU3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G07130.1.

Proteomic databases

PaxDbiQ9SFU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G07130.1; AT3G07130.1; AT3G07130.
GeneIDi819899.
GrameneiAT3G07130.1; AT3G07130.1; AT3G07130.
KEGGiath:AT3G07130.

Organism-specific databases

TAIRiAT3G07130.

Phylogenomic databases

eggNOGiKOG1378. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000238330.
InParanoidiQ9SFU3.
OMAiMHPLPGY.
OrthoDBiEOG093608JS.
PhylomeDBiQ9SFU3.

Enzyme and pathway databases

BioCyciARA:AT3G07130-MONOMER.
BRENDAi3.1.3.2. 399.

Miscellaneous databases

PROiQ9SFU3.

Gene expression databases

GenevisibleiQ9SFU3. AT.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
PF16656. Pur_ac_phosph_N. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPPA15_ARATH
AccessioniPrimary (citable) accession number: Q9SFU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.