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Q9SFU3

- PPA15_ARATH

UniProt

Q9SFU3 - PPA15_ARATH

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Protein
Purple acid phosphatase 15
Gene
PAP15, AT1, At3g07130, T1B9.21
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acid phosphatase activity with p-nitrophenyl phosphate (pNPP), D-myoinositol 1-phosphate (Ins1P1), phytic acid and Myo-inositol hexakisphosphate. Low or no activity with Glc-6-P and ATP. Confers shoot growth stimulation, enhanced salt and osmotic stress tolerance, and ABA insensitivity. May modulate ascorbic acid (AsA) levels by controlling the input of myoinositol into this branch of AsA biosynthesis.1 Publication

Catalytic activityi

Myo-inositol hexakisphosphate + H2O = myo-inositol pentakisphosphate + phosphate.
A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 iron ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

pH dependencei

Optimum pH is 4.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi194 – 1941Iron By similarity
Metal bindingi221 – 2211Iron By similarity
Metal bindingi221 – 2211Zinc By similarity
Metal bindingi224 – 2241Iron By similarity
Metal bindingi277 – 2771Zinc By similarity
Binding sitei277 – 2771Substrate By similarity
Metal bindingi359 – 3591Zinc By similarity
Active sitei369 – 3691Proton donor By similarity
Metal bindingi396 – 3961Zinc By similarity
Metal bindingi398 – 3981Iron By similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. pollen germination Source: TAIR
  3. seed germination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G07130-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 15 (EC:3.1.3.-, EC:3.1.3.2)
Alternative name(s):
Phytase
Gene namesi
Name:PAP15
Synonyms:AT1
Ordered Locus Names:At3g07130
ORF Names:T1B9.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G07130.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 532513Purple acid phosphatase 15
PRO_0000372819Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi136 – 1361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi200 – 2001N-linked (GlcNAc...) Reviewed prediction
Glycosylationi231 – 2311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi264 – 2641N-linked (GlcNAc...) Reviewed prediction
Glycosylationi286 – 2861N-linked (GlcNAc...) Reviewed prediction
Glycosylationi301 – 3011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi491 – 4911N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SFU3.
PRIDEiQ9SFU3.

Expressioni

Tissue specificityi

Expressed in roots, stems, cotyledons, leaves, flowers and siliques.2 Publications

Gene expression databases

GenevestigatoriQ9SFU3.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi3702.AT3G07130.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SFU3.
SMRiQ9SFU3. Positions 44-518.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni396 – 3983Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238330.
InParanoidiQ9SFU3.
OMAiMHPLPGY.
PhylomeDBiQ9SFU3.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SFU3-1 [UniParc]FASTAAdd to Basket

« Hide

MTFLLLLLFC FLSPAISSAH SIPSTLDGPF VPVTVPLDTS LRGQAIDLPD    50
TDPRVRRRVI GFEPEQISLS LSSDHDSIWV SWITGEFQIG KKVKPLDPTS 100
INSVVQFGTL RHSLSHEAKG HSLVYSQLYP FDGLLNYTSG IIHHVRITGL 150
KPSTIYYYRC GDPSRRAMSK IHHFRTMPVS SPSSYPGRIA VVGDLGLTYN 200
TTDTISHLIH NSPDLILLIG DVSYANLYLT NGTSSDCYSC SFPETPIHET 250
YQPRWDYWGR FMENLTSKVP LMVIEGNHEI ELQAENKTFE AYSSRFAFPF 300
NESGSSSTLY YSFNAGGIHF VMLGAYIAYD KSAEQYEWLK KDLAKVDRSV 350
TPWLVASWHP PWYSSYTAHY REAECMKEAM EELLYSYGTD IVFNGHVHAY 400
ERSNRVYNYE LDPCGPVYIV IGDGGNREKM AIEHADDPGK CPEPLTTPDP 450
VMGGFCAWNF TPSDKFCWDR QPDYSALRES SFGHGILEMK NETWALWTWY 500
RNQDSSSEVG DQIYIVRQPD RCPLHHRLVN HC 532
Length:532
Mass (Da):60,435
Last modified:May 1, 2000 - v1
Checksum:i658F6C88779444CD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF448726 mRNA. Translation: AAN74650.1.
AC012395 Genomic DNA. Translation: AAF20233.1.
CP002686 Genomic DNA. Translation: AEE74502.1.
RefSeqiNP_187369.1. NM_111593.2.
UniGeneiAt.40395.
At.71829.

Genome annotation databases

EnsemblPlantsiAT3G07130.1; AT3G07130.1; AT3G07130.
GeneIDi819899.
KEGGiath:AT3G07130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF448726 mRNA. Translation: AAN74650.1 .
AC012395 Genomic DNA. Translation: AAF20233.1 .
CP002686 Genomic DNA. Translation: AEE74502.1 .
RefSeqi NP_187369.1. NM_111593.2.
UniGenei At.40395.
At.71829.

3D structure databases

ProteinModelPortali Q9SFU3.
SMRi Q9SFU3. Positions 44-518.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G07130.1-P.

Proteomic databases

PaxDbi Q9SFU3.
PRIDEi Q9SFU3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G07130.1 ; AT3G07130.1 ; AT3G07130 .
GeneIDi 819899.
KEGGi ath:AT3G07130.

Organism-specific databases

TAIRi AT3G07130.

Phylogenomic databases

eggNOGi COG1409.
HOGENOMi HOG000238330.
InParanoidi Q9SFU3.
OMAi MHPLPGY.
PhylomeDBi Q9SFU3.

Enzyme and pathway databases

BioCyci ARA:AT3G07130-MONOMER.

Gene expression databases

Genevestigatori Q9SFU3.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "An Arabidopsis purple acid phosphatase with phytase activity increases foliar ascorbate."
    Zhang W., Gruszewski H.A., Chevone B.I., Nessler C.L.
    Plant Physiol. 146:431-440(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPPA15_ARATH
AccessioniPrimary (citable) accession number: Q9SFU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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