Reviewed, UniProtKB/Swiss-Prot Q9SFH9 (HEM2_ARATH)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase, chloroplastic
      Short name=ALADH
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	HEMB
Ordered Locus Names:	At1g69740
ORF Names:	T6C23.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Magnesium By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast By similarity
	Chain	? – 430		Delta-aminolevulinic acid dehydratase, chloroplastic		PRO_0000013314
Regions
	Region	219 – 237	19	Magnesium-binding By similarity
Sites
	Active site	351	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SFH9-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 44B0984247FC6147	FASTA	430	46,690
	10 20 30 40 50 60 MATTPIFNAS CSFPSTRGID CKSYIGLRSN VSKVSVASSR IATSQRRNLV VRASESGNGH 70 80 90 100 110 120 AKKLGMSDAE CEAAVAAGNV PEAPPVPPKP AAPVGTPIIK PLNLSRRPRR NRASPVTRAA 130 140 150 160 170 180 FQETDISPAN FVYPLFIHEG EEDTPIGAMP GCYRLGWRHG LVQEVAKARA VGVNSIVLFP 190 200 210 220 230 240 KVPEALKNST GDEAYNDNGL VPRTIRLLKD KYPDLIIYTD VALDPYSSDG HDGIVREDGV 250 260 270 280 290 300 IMNDETVHQL CKQAVSQARA GADVVSPSDM MDGRVGAIRS ALDAEGFQNV SIMSYTAKYA 310 320 330 340 350 360 SSFYGPFREA LDSNPRFGDK KTYQMNPANY REALIEARED EAEGADILLV KPGLPYLDII 370 380 390 400 410 420 RLLRDKSPLP IAAYQVSGEY SMIKAGGVLK MIDEEKVMME SLMCLRRAGA DIILTYFALQ 430 AATCLCGEKR

References

[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC013289 Genomic DNA. Translation: AAG52549.1. AF327428 mRNA. Translation: AAG42018.1. AF361803 mRNA. Translation: AAK32816.1. AY059154 mRNA. Translation: AAL15379.1. AY081254 mRNA. Translation: AAL91143.1. AY113941 mRNA. Translation: AAM44989.1. AY120705 mRNA. Translation: AAM53263.1. AY128711 mRNA. Translation: AAM91111.1.
IPI	IPI00537155.
PIR	D96719.
RefSeq	NP_001077800.1. NP_177132.1.
UniGene	At.10020 At.21048
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SFH9.
Proteomic databases
PRIDE	Q9SFH9.
ProMEX	Q9SFH9.
Genome annotation databases
GeneID	843310.
GenomeReviews	Gene locus AT1G69740 in contig CT485782_GR.
KEGG	ath:AT1G69740.
NMPDR	fig|3702.1.peg.6446.
Organism-specific databases
TAIR	At1g69740.
Phylogenomic databases
eggNOG	KOG2794.
HOGENOM	HBG285270.
InParanoid	Q9SFH9.
OMA	DPFTSHG.
PhylomeDB	Q9SFH9.
Enzyme and pathway databases
BRENDA	4.2.1.24. 302.
Gene expression databases
ArrayExpress	Q9SFH9.
Genevestigator	Q9SFH9.
GermOnline	AT1G69740. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PRINTS	PR00144. DALDHYDRTASE.
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_ARATH

Accession

Primary (citable) accession number: Q9SFH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)