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Q9SFH9

- HEM21_ARATH

UniProt

Q9SFH9 - HEM21_ARATH

Protein

Delta-aminolevulinic acid dehydratase 1, chloroplastic

Gene

HEMB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei298 – 2981Schiff-base intermediate with substrateBy similarity
    Binding sitei308 – 3081Substrate 1By similarity
    Binding sitei320 – 3201Substrate 1By similarity
    Metal bindingi336 – 3361MagnesiumBy similarity
    Active sitei351 – 3511Schiff-base intermediate with substrateBy similarity
    Binding sitei377 – 3771Substrate 2By similarity
    Binding sitei416 – 4161Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
    2. porphyrin-containing compound biosynthetic process Source: TAIR
    3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-2417-MONOMER.
    UniPathwayiUPA00251; UER00318.
    UPA00668.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase 1, chloroplastic (EC:4.2.1.24)
    Short name:
    ALADH1
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:HEMB1
    Ordered Locus Names:At1g69740
    ORF Names:T6C23.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G69740.

    Subcellular locationi

    Plastidchloroplast By similarity

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Embryo lethal when homozygous. Impaired plant growth and development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5252ChloroplastSequence AnalysisAdd
    BLAST
    Chaini53 – 430378Delta-aminolevulinic acid dehydratase 1, chloroplasticPRO_0000013314Add
    BLAST

    Proteomic databases

    PaxDbiQ9SFH9.
    PRIDEiQ9SFH9.

    Expressioni

    Tissue specificityi

    Highly expressed in cotyledons during dark-to-light transition.1 Publication

    Inductioni

    Up-regulated by the transcription factors FAR1 and FHY3.1 Publication

    Gene expression databases

    GenevestigatoriQ9SFH9.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    BioGridi28531. 1 interaction.
    STRINGi3702.AT1G69740.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SFH9.
    SMRiQ9SFH9. Positions 102-422.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi81 – 10121Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0113.
    HOGENOMiHOG000020323.
    InParanoidiQ9SFH9.
    KOiK01698.
    OMAiDMILTYF.
    PhylomeDBiQ9SFH9.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SFH9-1 [UniParc]FASTAAdd to Basket

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    MATTPIFNAS CSFPSTRGID CKSYIGLRSN VSKVSVASSR IATSQRRNLV    50
    VRASESGNGH AKKLGMSDAE CEAAVAAGNV PEAPPVPPKP AAPVGTPIIK 100
    PLNLSRRPRR NRASPVTRAA FQETDISPAN FVYPLFIHEG EEDTPIGAMP 150
    GCYRLGWRHG LVQEVAKARA VGVNSIVLFP KVPEALKNST GDEAYNDNGL 200
    VPRTIRLLKD KYPDLIIYTD VALDPYSSDG HDGIVREDGV IMNDETVHQL 250
    CKQAVSQARA GADVVSPSDM MDGRVGAIRS ALDAEGFQNV SIMSYTAKYA 300
    SSFYGPFREA LDSNPRFGDK KTYQMNPANY REALIEARED EAEGADILLV 350
    KPGLPYLDII RLLRDKSPLP IAAYQVSGEY SMIKAGGVLK MIDEEKVMME 400
    SLMCLRRAGA DIILTYFALQ AATCLCGEKR 430
    Length:430
    Mass (Da):46,690
    Last modified:May 1, 2000 - v1
    Checksum:i44B0984247FC6147
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC013289 Genomic DNA. Translation: AAG52549.1.
    CP002684 Genomic DNA. Translation: AEE34969.1.
    CP002684 Genomic DNA. Translation: AEE34970.1.
    AF327428 mRNA. Translation: AAG42018.1.
    AF361803 mRNA. Translation: AAK32816.1.
    AY059154 mRNA. Translation: AAL15379.1.
    AY081254 mRNA. Translation: AAL91143.1.
    AY113941 mRNA. Translation: AAM44989.1.
    AY120705 mRNA. Translation: AAM53263.1.
    AY128711 mRNA. Translation: AAM91111.1.
    PIRiD96719.
    RefSeqiNP_001077800.1. NM_001084331.2.
    NP_177132.1. NM_105642.3.
    UniGeneiAt.10020.
    At.21048.

    Genome annotation databases

    EnsemblPlantsiAT1G69740.1; AT1G69740.1; AT1G69740.
    AT1G69740.2; AT1G69740.2; AT1G69740.
    GeneIDi843310.
    KEGGiath:AT1G69740.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC013289 Genomic DNA. Translation: AAG52549.1 .
    CP002684 Genomic DNA. Translation: AEE34969.1 .
    CP002684 Genomic DNA. Translation: AEE34970.1 .
    AF327428 mRNA. Translation: AAG42018.1 .
    AF361803 mRNA. Translation: AAK32816.1 .
    AY059154 mRNA. Translation: AAL15379.1 .
    AY081254 mRNA. Translation: AAL91143.1 .
    AY113941 mRNA. Translation: AAM44989.1 .
    AY120705 mRNA. Translation: AAM53263.1 .
    AY128711 mRNA. Translation: AAM91111.1 .
    PIRi D96719.
    RefSeqi NP_001077800.1. NM_001084331.2.
    NP_177132.1. NM_105642.3.
    UniGenei At.10020.
    At.21048.

    3D structure databases

    ProteinModelPortali Q9SFH9.
    SMRi Q9SFH9. Positions 102-422.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 28531. 1 interaction.
    STRINGi 3702.AT1G69740.2-P.

    Proteomic databases

    PaxDbi Q9SFH9.
    PRIDEi Q9SFH9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G69740.1 ; AT1G69740.1 ; AT1G69740 .
    AT1G69740.2 ; AT1G69740.2 ; AT1G69740 .
    GeneIDi 843310.
    KEGGi ath:AT1G69740.

    Organism-specific databases

    TAIRi AT1G69740.

    Phylogenomic databases

    eggNOGi COG0113.
    HOGENOMi HOG000020323.
    InParanoidi Q9SFH9.
    KOi K01698.
    OMAi DMILTYF.
    PhylomeDBi Q9SFH9.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .
    UPA00668 .
    BioCyci ARA:GQT-2417-MONOMER.

    Miscellaneous databases

    PROi Q9SFH9.

    Gene expression databases

    Genevestigatori Q9SFH9.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Transposase-derived proteins FHY3/FAR1 interact with PHYTOCHROME-INTERACTING FACTOR1 to regulate chlorophyll biosynthesis by modulating HEMB1 during deetiolation in Arabidopsis."
      Tang W., Wang W., Chen D., Ji Q., Jing Y., Wang H., Lin R.
      Plant Cell 24:1984-2000(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY FHY3 AND FAR1, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiHEM21_ARATH
    AccessioniPrimary (citable) accession number: Q9SFH9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3