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Q9SFH9

- HEM21_ARATH

UniProt

Q9SFH9 - HEM21_ARATH

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Protein

Delta-aminolevulinic acid dehydratase 1, chloroplastic

Gene
HEMB1, At1g69740, T6C23.6
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei298 – 2981Schiff-base intermediate with substrate By similarity
Binding sitei308 – 3081Substrate 1 By similarity
Binding sitei320 – 3201Substrate 1 By similarity
Metal bindingi336 – 3361Magnesium By similarity
Active sitei351 – 3511Schiff-base intermediate with substrate By similarity
Binding sitei377 – 3771Substrate 2 By similarity
Binding sitei416 – 4161Substrate 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  2. porphyrin-containing compound biosynthetic process Source: TAIR
  3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:GQT-2417-MONOMER.
UniPathwayiUPA00251; UER00318.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase 1, chloroplastic (EC:4.2.1.24)
Short name:
ALADH1
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:HEMB1
Ordered Locus Names:At1g69740
ORF Names:T6C23.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G69740.

Subcellular locationi

Plastidchloroplast By similarity

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryo lethal when homozygous. Impaired plant growth and development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5252Chloroplast Reviewed predictionAdd
BLAST
Chaini53 – 430378Delta-aminolevulinic acid dehydratase 1, chloroplasticPRO_0000013314Add
BLAST

Proteomic databases

PaxDbiQ9SFH9.
PRIDEiQ9SFH9.

Expressioni

Tissue specificityi

Highly expressed in cotyledons during dark-to-light transition.1 Publication

Inductioni

Up-regulated by the transcription factors FAR1 and FHY3.1 Publication

Gene expression databases

GenevestigatoriQ9SFH9.

Interactioni

Subunit structurei

Homooctamer By similarity.

Protein-protein interaction databases

BioGridi28531. 1 interaction.
STRINGi3702.AT1G69740.2-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SFH9.
SMRiQ9SFH9. Positions 102-422.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi81 – 10121Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the ALADH family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
InParanoidiQ9SFH9.
KOiK01698.
OMAiDMILTYF.
PhylomeDBiQ9SFH9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SFH9-1 [UniParc]FASTAAdd to Basket

« Hide

MATTPIFNAS CSFPSTRGID CKSYIGLRSN VSKVSVASSR IATSQRRNLV    50
VRASESGNGH AKKLGMSDAE CEAAVAAGNV PEAPPVPPKP AAPVGTPIIK 100
PLNLSRRPRR NRASPVTRAA FQETDISPAN FVYPLFIHEG EEDTPIGAMP 150
GCYRLGWRHG LVQEVAKARA VGVNSIVLFP KVPEALKNST GDEAYNDNGL 200
VPRTIRLLKD KYPDLIIYTD VALDPYSSDG HDGIVREDGV IMNDETVHQL 250
CKQAVSQARA GADVVSPSDM MDGRVGAIRS ALDAEGFQNV SIMSYTAKYA 300
SSFYGPFREA LDSNPRFGDK KTYQMNPANY REALIEARED EAEGADILLV 350
KPGLPYLDII RLLRDKSPLP IAAYQVSGEY SMIKAGGVLK MIDEEKVMME 400
SLMCLRRAGA DIILTYFALQ AATCLCGEKR 430
Length:430
Mass (Da):46,690
Last modified:May 1, 2000 - v1
Checksum:i44B0984247FC6147
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC013289 Genomic DNA. Translation: AAG52549.1.
CP002684 Genomic DNA. Translation: AEE34969.1.
CP002684 Genomic DNA. Translation: AEE34970.1.
AF327428 mRNA. Translation: AAG42018.1.
AF361803 mRNA. Translation: AAK32816.1.
AY059154 mRNA. Translation: AAL15379.1.
AY081254 mRNA. Translation: AAL91143.1.
AY113941 mRNA. Translation: AAM44989.1.
AY120705 mRNA. Translation: AAM53263.1.
AY128711 mRNA. Translation: AAM91111.1.
PIRiD96719.
RefSeqiNP_001077800.1. NM_001084331.2.
NP_177132.1. NM_105642.3.
UniGeneiAt.10020.
At.21048.

Genome annotation databases

EnsemblPlantsiAT1G69740.1; AT1G69740.1; AT1G69740.
AT1G69740.2; AT1G69740.2; AT1G69740.
GeneIDi843310.
KEGGiath:AT1G69740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC013289 Genomic DNA. Translation: AAG52549.1 .
CP002684 Genomic DNA. Translation: AEE34969.1 .
CP002684 Genomic DNA. Translation: AEE34970.1 .
AF327428 mRNA. Translation: AAG42018.1 .
AF361803 mRNA. Translation: AAK32816.1 .
AY059154 mRNA. Translation: AAL15379.1 .
AY081254 mRNA. Translation: AAL91143.1 .
AY113941 mRNA. Translation: AAM44989.1 .
AY120705 mRNA. Translation: AAM53263.1 .
AY128711 mRNA. Translation: AAM91111.1 .
PIRi D96719.
RefSeqi NP_001077800.1. NM_001084331.2.
NP_177132.1. NM_105642.3.
UniGenei At.10020.
At.21048.

3D structure databases

ProteinModelPortali Q9SFH9.
SMRi Q9SFH9. Positions 102-422.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 28531. 1 interaction.
STRINGi 3702.AT1G69740.2-P.

Proteomic databases

PaxDbi Q9SFH9.
PRIDEi Q9SFH9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G69740.1 ; AT1G69740.1 ; AT1G69740 .
AT1G69740.2 ; AT1G69740.2 ; AT1G69740 .
GeneIDi 843310.
KEGGi ath:AT1G69740.

Organism-specific databases

TAIRi AT1G69740.

Phylogenomic databases

eggNOGi COG0113.
HOGENOMi HOG000020323.
InParanoidi Q9SFH9.
KOi K01698.
OMAi DMILTYF.
PhylomeDBi Q9SFH9.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .
UPA00668 .
BioCyci ARA:GQT-2417-MONOMER.

Miscellaneous databases

PROi Q9SFH9.

Gene expression databases

Genevestigatori Q9SFH9.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Transposase-derived proteins FHY3/FAR1 interact with PHYTOCHROME-INTERACTING FACTOR1 to regulate chlorophyll biosynthesis by modulating HEMB1 during deetiolation in Arabidopsis."
    Tang W., Wang W., Chen D., Ji Q., Jing Y., Wang H., Lin R.
    Plant Cell 24:1984-2000(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY FHY3 AND FAR1, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiHEM21_ARATH
AccessioniPrimary (citable) accession number: Q9SFH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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