ID   PI5K6_ARATH             Reviewed;         715 AA.
AC   Q9SFB8;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   27-MAR-2024, entry version 147.
DE   RecName: Full=Phosphatidylinositol 4-phosphate 5-kinase 6;
DE            Short=AtPIP5K6;
DE            EC=2.7.1.68;
DE   AltName: Full=1-phosphatidylinositol 4-phosphate kinase 6;
DE   AltName: Full=Diphosphoinositide kinase 6;
DE   AltName: Full=PtdIns(4)P-5-kinase 6;
GN   Name=PIP5K6; OrderedLocusNames=At3g07960; ORFNames=F17A17.30;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12226484; DOI=10.1104/pp.004770;
RA   Mueller-Roeber B., Pical C.;
RT   "Inositol phospholipid metabolism in Arabidopsis. Characterized and
RT   putative isoforms of inositol phospholipid kinase and phosphoinositide-
RT   specific phospholipase C.";
RL   Plant Physiol. 130:22-46(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-
CC         phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
CC         inositol-4,5-bisphosphate) + ADP + H(+); Xref=Rhea:RHEA:14425,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:58178,
CC         ChEBI:CHEBI:58456, ChEBI:CHEBI:456216; EC=2.7.1.68;
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DR   EMBL; AC013483; AAF21206.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE74625.1; -; Genomic_DNA.
DR   EMBL; BT008595; AAP40421.1; -; mRNA.
DR   RefSeq; NP_187453.1; NM_111675.6.
DR   AlphaFoldDB; Q9SFB8; -.
DR   SMR; Q9SFB8; -.
DR   BioGRID; 5322; 1.
DR   STRING; 3702.Q9SFB8; -.
DR   iPTMnet; Q9SFB8; -.
DR   PaxDb; 3702-AT3G07960-1; -.
DR   ProteomicsDB; 234955; -.
DR   EnsemblPlants; AT3G07960.1; AT3G07960.1; AT3G07960.
DR   GeneID; 819987; -.
DR   Gramene; AT3G07960.1; AT3G07960.1; AT3G07960.
DR   KEGG; ath:AT3G07960; -.
DR   Araport; AT3G07960; -.
DR   TAIR; AT3G07960; PIP5K6.
DR   eggNOG; KOG0229; Eukaryota.
DR   HOGENOM; CLU_004312_6_4_1; -.
DR   InParanoid; Q9SFB8; -.
DR   OrthoDB; 340426at2759; -.
DR   PhylomeDB; Q9SFB8; -.
DR   BioCyc; ARA:AT3G07960-MONOMER; -.
DR   PRO; PR:Q9SFB8; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9SFB8; baseline and differential.
DR   GO; GO:0016324; C:apical plasma membrane; IDA:TAIR.
DR   GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR   GO; GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase activity; IDA:TAIR.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0072583; P:clathrin-dependent endocytosis; IMP:TAIR.
DR   GO; GO:0046488; P:phosphatidylinositol metabolic process; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd17302; PIPKc_AtPIP5K_like; 1.
DR   Gene3D; 3.30.810.10; 2-Layer Sandwich; 1.
DR   Gene3D; 2.20.110.10; Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain; 3.
DR   Gene3D; 3.30.800.10; Phosphatidylinositol Phosphate Kinase II Beta; 1.
DR   InterPro; IPR003409; MORN.
DR   InterPro; IPR017163; PIno-4-P-5_kinase_pln.
DR   InterPro; IPR027483; PInositol-4-P-4/5-kinase_C_sf.
DR   InterPro; IPR002498; PInositol-4-P-4/5-kinase_core.
DR   InterPro; IPR027484; PInositol-4-P-5-kinase_N.
DR   InterPro; IPR023610; PInositol-4/5-P-5/4-kinase.
DR   PANTHER; PTHR23086:SF113; PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE 6; 1.
DR   PANTHER; PTHR23086; PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE; 1.
DR   Pfam; PF02493; MORN; 7.
DR   Pfam; PF01504; PIP5K; 1.
DR   PIRSF; PIRSF037274; PIP5K_plant_prd; 1.
DR   SMART; SM00698; MORN; 7.
DR   SMART; SM00330; PIPKc; 1.
DR   SUPFAM; SSF82185; Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain; 2.
DR   SUPFAM; SSF56104; SAICAR synthase-like; 1.
DR   PROSITE; PS51455; PIPK; 1.
DR   Genevisible; Q9SFB8; AT.
PE   2: Evidence at transcript level;
KW   ATP-binding; Kinase; Nucleotide-binding; Reference proteome; Repeat;
KW   Transferase.
FT   CHAIN           1..715
FT                   /note="Phosphatidylinositol 4-phosphate 5-kinase 6"
FT                   /id="PRO_0000185478"
FT   REPEAT          32..54
FT                   /note="MORN 1"
FT   REPEAT          55..77
FT                   /note="MORN 2"
FT   REPEAT          78..100
FT                   /note="MORN 3"
FT   REPEAT          101..123
FT                   /note="MORN 4"
FT   REPEAT          124..146
FT                   /note="MORN 5"
FT   REPEAT          147..169
FT                   /note="MORN 6"
FT   REPEAT          170..192
FT                   /note="MORN 7"
FT   REPEAT          193..214
FT                   /note="MORN 8"
FT   DOMAIN          321..711
FT                   /note="PIPK"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00781"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          253..306
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          671..692
FT                   /note="Activation loop"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        265..280
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        284..306
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   715 AA;  81422 MW;  795D86CEB1FB52E9 CRC64;
     MSVAHADDAD DYSRPTGESY HAEKALPSGD FYTGQWRDNL PHGHGKYLWT DGCMYVGDWH
     RGKTMGKGRF SWPSGATYEG DFKNGYMDGK GTYIDSSGDL YRGSWVMNLR HGQGTKSYVN
     GDCYDGEWRR GLQDGHGRYQ WKNENHYIGQ WKNGLMNGNG TMIWSNGNRY DGSWEDGAPK
     GNGTFRWSDG SFYVGVWSKD PKEQNGTYYP STSSGNFDWQ PQQVFYVDLS ECVVCTCQRI
     PVLPSQKMPV WYGASEQSSS GNRTKNSERP RRRSVDGRVS NGEMELRSNG SGYLQVDDNA
     ESTRSSLGPL RIQPAKKQGQ TISKGHKNYE LMLNLQLGIR HSVGRPAPAT SLDLKASAFD
     PKEKLWTKFP SEGSKYTPPH QSCEFKWKDY CPVVFRTLRK LFSVDAADYM LSICGNDALR
     ELSSPGKSGS FFYLTNDDRY MIKTMKKAET KVLIRMLPAY YNHVRACENT LVTKFFGLHC
     VKLTGTAQKK VRFVIMGNLF CTGHSIHRRF DLKGSSHGRL TTKPESEIDP NTTLKDLDLN
     FAFRLQKNWF QEFCRQVDRD CEFLEQERIM DYSLLVGLHF REAAIKDSAT PTSGARTPTG
     NSETRLSRAE MDRFLLDASK LASIKLGINM PARVERTARR SDCENQLVGD PTGEFYDVIV
     YFGIIDILQD YDISKKLEHA YKSMQYDPTS ISAVDPKQYS RRFRDFIFRV FVEDA
//