Q9SFB7 (QRT2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polygalacturonase QRT2
Short name=AtQRT2
Short name=PG QRT2
EC=3.2.1.15

Alternative name(s):
Pectinase QRT2
Protein QUARTET 2

Gene names

Name:	QRT2
Ordered Locus Names:	At3g07970
ORF Names:	F17A17.31

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Polygalacturonase required for cell type-specific pectin degradation to separate microspores. Involved in anther dehiscence and floral organ abscission. Ref.3 Ref.6 Ref.7
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed predominantly in roots with lower expression levels in rosette leaves, flower buds and siliques. Bearly detected in seeds. Found in flowers undergoing floral organ abscission. Also expressed early in anther development, at the time of microspore separation. Ref.4 Ref.5 Ref.6 Ref.7
Disruption phenotype	The mature pollen grains are arranged in a tetrad. No visible phenotype regarding floral organ abscission. Ref.4 Ref.7
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 4 PbH1 repeats.
Sequence caution	The sequence AAF21207.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anther dehiscence Inferred from mutant phenotype Ref.7. Source: TAIR cell wall modification involved in abscission Traceable author statement Ref.7. Source: TAIR fruit dehiscence Inferred from mutant phenotype Ref.7. Source: TAIR pectin catabolic process Inferred from mutant phenotype. Source: TAIR pollen sperm cell differentiation Inferred from mutant phenotype Ref.3. Source: TAIR
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	polygalacturonase activity Inferred from direct assay Ref.7. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 439	418	Polygalacturonase QRT2		PRO_0000367915
Regions
	Repeat	201 – 250	50	PbH1 1
	Repeat	251 – 272	22	PbH1 2
	Repeat	304 – 325	22	PbH1 3
	Repeat	333 – 354	22	PbH1 4
Sites
	Active site	265	1	Proton donor By similarity
	Active site	288	1	By similarity
Experimental info
	Mutagenesis	372	1	V → A in qrt2-1; loss of function. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9SFB7 [UniParc]. Last modified April 14, 2009. Version 2. Checksum: D52F4691C95633A4	FASTA	439	48,572
	10 20 30 40 50 60 MYEKIIILSV FLLTFLPSCF SSYPFNHRDD LFMSSNVYYE TNRQHQHGHN TRNSHLKNRH 70 80 90 100 110 120 GYAPRSSPRS FNVNTFGAKA NGNDDSKAFM KAWEAACSST GIVYIVAPKN RDYMLKAVTF 130 140 150 160 170 180 SGPCKSSLII FKIYGRIEAW ENPSDYKERR HWIVFENVNN LRVEGGGRID GNGHIWWPKS 190 200 210 220 230 240 CKINPQLPCL GAPTAVTFVE CNNLRVSNIR LENAQQMHLT FQDCKNVKAL NLMVTSPADS 250 260 270 280 290 300 PNTDGIHVSG TQNILIQDSI VRTGDDCISI VSGSENVRAT GITCGPGHGI SIGSLGEDNS 310 320 330 340 350 360 EAYVSNVVVN KATLIGTTNG VRIKTWQGGH GMAKNIIFQD IIMKNVTNPI IINQDYCDRV 370 380 390 400 410 420 EACPEQKSAV QVSNVLYKNI QGTSSRPIAV KFVCSKNIPC RGISMQNVKL VDQTQQDVSK 430 ASCSNVKLDT RGNVSPLCT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Tetrad analysis possible in Arabidopsis with mutation of the QUARTET (QRT) genes." Preuss D., Rhee S.Y., Davis R.W. Science 264:1458-1460(1994) [PubMed: 8197459] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF VAL-372.
[4]	"Expression divergence and functional redundancy of polygalacturonases in floral organ abscission." Kim J., Patterson S.E. Plant Signal. Behav. 1:281-283(2006) Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[5]	"Patterns of expansion and expression divergence in the plant polygalacturonase gene family." Kim J., Shiu S.-H., Thoma S., Li W.-H., Patterson S.E. Genome Biol. 7:R87.1-R87.14(2006) [PubMed: 17010199] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana." Gonzalez-Carranza Z.H., Elliott K.A., Roberts J.A. J. Exp. Bot. 58:3719-3730(2007) [PubMed: 17928369] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 are polygalacturonases required for cell separation during reproductive development in Arabidopsis." Ogawa M., Kay P., Wilson S., Swain S.M. Plant Cell 21:216-233(2009) [PubMed: 19168715] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC013483 Genomic DNA. Translation: AAF21207.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE74626.1.
IPI	IPI00516594.
RefSeq	NP_187454.2. NM_111676.3.
UniGene	At.40276.
3D structure databases
ProteinModelPortal	Q9SFB7.
SMR	Q9SFB7. Positions 83-428.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SFB7.
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Proteomic databases
PRIDE	Q9SFB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G07970.1; AT3G07970.1; AT3G07970.
GeneID	819988.
GenomeReviews	Gene locus AT3G07970 in contig BA000014_GR.
KEGG	ath:AT3G07970.
Organism-specific databases
TAIR	At3g07970.
Phylogenomic databases
eggNOG	euNOG04659.
GeneTree	EPGT00050000001253.
HOGENOM	HBG744658.
InParanoid	Q9SFB7.
OMA	NIRLENA.
PhylomeDB	Q9SFB7.
ProtClustDB	CLSN2690525.
Gene expression databases
Genevestigator	Q9SFB7.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 4 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

QRT2_ARATH

Accession

Primary (citable) accession number: Q9SFB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 14, 2009
Last modified:	December 14, 2011
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program