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Protein

Polygalacturonase QRT2

Gene

QRT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Polygalacturonase required for cell type-specific pectin degradation to separate microspores. Involved in anther dehiscence and floral organ abscission.3 Publications

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei265 – 2651Proton donorPROSITE-ProRule annotation
Active sitei288 – 2881PROSITE-ProRule annotation

GO - Molecular functioni

  • polygalacturonase activity Source: TAIR

GO - Biological processi

  • anther dehiscence Source: TAIR
  • cell wall modification involved in abscission Source: TAIR
  • fruit dehiscence Source: TAIR
  • pectin catabolic process Source: TAIR
  • pollen sperm cell differentiation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT3G07970-MONOMER.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase QRT2 (EC:3.2.1.15)
Short name:
AtQRT2
Short name:
PG QRT2
Alternative name(s):
Pectinase QRT2
Protein QUARTET 2
Gene namesi
Name:QRT2
Ordered Locus Names:At3g07970
ORF Names:F17A17.31
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G07970.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

The mature pollen grains are arranged in a tetrad. No visible phenotype regarding floral organ abscission.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi372 – 3721V → A in qrt2-1; loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 439418Polygalacturonase QRT2PRO_0000367915Add
BLAST

Proteomic databases

PaxDbiQ9SFB7.
PRIDEiQ9SFB7.

Expressioni

Tissue specificityi

Expressed predominantly in roots with lower expression levels in rosette leaves, flower buds and siliques. Bearly detected in seeds. Found in flowers undergoing floral organ abscission. Also expressed early in anther development, at the time of microspore separation.4 Publications

Gene expression databases

GenevisibleiQ9SFB7. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G07970.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SFB7.
SMRiQ9SFB7. Positions 85-416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati201 – 25050PbH1 1Add
BLAST
Repeati251 – 27222PbH1 2Add
BLAST
Repeati304 – 32522PbH1 3Add
BLAST
Repeati333 – 35422PbH1 4Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 4 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IF8F. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ9SFB7.
OMAiIFQDIIM.
OrthoDBiEOG09360ADQ.
PhylomeDBiQ9SFB7.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 4 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SFB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEKIIILSV FLLTFLPSCF SSYPFNHRDD LFMSSNVYYE TNRQHQHGHN
60 70 80 90 100
TRNSHLKNRH GYAPRSSPRS FNVNTFGAKA NGNDDSKAFM KAWEAACSST
110 120 130 140 150
GIVYIVAPKN RDYMLKAVTF SGPCKSSLII FKIYGRIEAW ENPSDYKERR
160 170 180 190 200
HWIVFENVNN LRVEGGGRID GNGHIWWPKS CKINPQLPCL GAPTAVTFVE
210 220 230 240 250
CNNLRVSNIR LENAQQMHLT FQDCKNVKAL NLMVTSPADS PNTDGIHVSG
260 270 280 290 300
TQNILIQDSI VRTGDDCISI VSGSENVRAT GITCGPGHGI SIGSLGEDNS
310 320 330 340 350
EAYVSNVVVN KATLIGTTNG VRIKTWQGGH GMAKNIIFQD IIMKNVTNPI
360 370 380 390 400
IINQDYCDRV EACPEQKSAV QVSNVLYKNI QGTSSRPIAV KFVCSKNIPC
410 420 430
RGISMQNVKL VDQTQQDVSK ASCSNVKLDT RGNVSPLCT
Length:439
Mass (Da):48,572
Last modified:April 14, 2009 - v2
Checksum:iD52F4691C95633A4
GO

Sequence cautioni

The sequence AAF21207 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013483 Genomic DNA. Translation: AAF21207.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74626.1.
RefSeqiNP_187454.2. NM_111676.3.
UniGeneiAt.40276.

Genome annotation databases

EnsemblPlantsiAT3G07970.1; AT3G07970.1; AT3G07970.
GeneIDi819988.
GrameneiAT3G07970.1; AT3G07970.1; AT3G07970.
KEGGiath:AT3G07970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013483 Genomic DNA. Translation: AAF21207.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74626.1.
RefSeqiNP_187454.2. NM_111676.3.
UniGeneiAt.40276.

3D structure databases

ProteinModelPortaliQ9SFB7.
SMRiQ9SFB7. Positions 85-416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G07970.1.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Proteomic databases

PaxDbiQ9SFB7.
PRIDEiQ9SFB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G07970.1; AT3G07970.1; AT3G07970.
GeneIDi819988.
GrameneiAT3G07970.1; AT3G07970.1; AT3G07970.
KEGGiath:AT3G07970.

Organism-specific databases

TAIRiAT3G07970.

Phylogenomic databases

eggNOGiENOG410IF8F. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ9SFB7.
OMAiIFQDIIM.
OrthoDBiEOG09360ADQ.
PhylomeDBiQ9SFB7.

Enzyme and pathway databases

BioCyciARA:AT3G07970-MONOMER.

Miscellaneous databases

PROiQ9SFB7.

Gene expression databases

GenevisibleiQ9SFB7. AT.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 4 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQRT2_ARATH
AccessioniPrimary (citable) accession number: Q9SFB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: September 7, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.