Q9SF85 (ADK1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine kinase 1
Short name=AK 1
EC=2.7.1.20

Alternative name(s):
Adenosine 5'-phosphotransferase 1

Gene names

Name:	ADK1
Ordered Locus Names:	At3g09820
ORF Names:	F8A24.13

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Essential to sustain methyl recycling. Ref.7
Catalytic activity	ATP + adenosine = ADP + AMP.
Cofactor	Magnesium By similarity.
Enzyme regulation	Inactivated by the begomovirus AL2 protein or the curtovirus L2 protein By similarity.
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.
Subunit structure	Interacts with the begomovirus AL2 protein and the curtovirus L2 protein. Ref.6
Tissue specificity	Ubiquitous. Ref.1 Ref.7
Developmental stage	Up-regulated during the lignification process in inflorescence stems. Ref.7
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

Ontologies

Keywords
Biological process	Host-virus interaction Purine salvage
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	adenosine salvage Traceable author statement. Source: TAIR interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR response to trehalose-6-phosphate stimulus Inferred from expression pattern. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast Inferred from direct assay. Source: TAIR cytosol Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosine kinase activity Inferred from direct assay Ref.1. Source: TAIR copper ion binding Inferred from direct assay. Source: TAIR phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9SF85-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9SF85-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.
     43-49: EDKHLPM → MIIGMFR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Adenosine kinase 1		PRO_0000080057
Sites
	Active site	299	1	By similarity
Natural variations
	Alternative sequence	1 – 42	42	Missing in isoform 2.		VSP_040515
	Alternative sequence	43 – 49	7	EDKHLPM → MIIGMFR in isoform 2.		VSP_040516

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 771C789CD1D2D2E6	FASTA	344	37,836
	10 20 30 40 50 60 MASSDFDGIL LGMGNPLLDV SAVVDQQFLD KYDIKLNNAI LAEDKHLPMY DEMSQKFNVE 70 80 90 100 110 120 YIAGGATQNS IKVAQWMLQV PGATSYMGSI GKDKYGEAMK KDATAAGVYV HYYEDEATPT 130 140 150 160 170 180 GTCGVCVLGG ERSLIANLSA ANCYKVEHLK KPENWALVEK AKFYYIAGFF LTVSPESIQL 190 200 210 220 230 240 VREHAAANNK VFTMNLSAPF ICEFFKDVQE KCLPYMDYIF GNETEARTFS RVHGWETDDV 250 260 270 280 290 300 EQIAIKMSQL PKASGTYKRT TVITQGADPV VVAEDGKVKK YPVIPLPKEK LVDTNGAGDA 310 320 330 340 FVGGFLSQLV HGKGIEECVR AGCYASNVVI QRSGCTYPEK PDFN
	Isoform 2 [UniParc]. Checksum: FBFC933E0993D84D	FASTA	302	33,326
	10 20 30 40 50 60 MIIGMFRYDE MSQKFNVEYI AGGATQNSIK VAQWMLQVPG ATSYMGSIGK DKYGEAMKKD 70 80 90 100 110 120 ATAAGVYVHY YEDEATPTGT CGVCVLGGER SLIANLSAAN CYKVEHLKKP ENWALVEKAK 130 140 150 160 170 180 FYYIAGFFLT VSPESIQLVR EHAAANNKVF TMNLSAPFIC EFFKDVQEKC LPYMDYIFGN 190 200 210 220 230 240 ETEARTFSRV HGWETDDVEQ IAIKMSQLPK ASGTYKRTTV ITQGADPVVV AEDGKVKKYP 250 260 270 280 290 300 VIPLPKEKLV DTNGAGDAFV GGFLSQLVHG KGIEECVRAG CYASNVVIQR SGCTYPEKPD FN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Adenosine kinase of Arabidopsis. Kinetic properties and gene expression." Moffatt B.A., Wang L., Allen M.S., Stevens Y.Y., Qin W., Snider J., von Schwartzenberg K. Plant Physiol. 124:1775-1785(2000) [PubMed: 11115893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CHARACTERIZATION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Arabidopsis ORF clones." De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[6]	"Adenosine kinase is inactivated by geminivirus AL2 and L2 proteins." Wang H., Hao L., Shung C.-Y., Sunter G., Bisaro D.M. Plant Cell 15:3020-3032(2003) [PubMed: 14615595] [Abstract] Cited for: INTERACTION WITH TOMATO GOLDEN MOSAIC VIRUS AL2 AND BEET CURLY TOP VIRUS L2.
[7]	"Methyl recycling activities are co-ordinately regulated during plant development." Pereira L.A.R., Todorova M., Cai X., Makaroff C.A., Emery R.J.N., Moffatt B.A. J. Exp. Bot. 58:1083-1098(2007) [PubMed: 17272833] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF180896 Genomic DNA. Translation: AAG45248.1. AF180894 mRNA. Translation: AAG45246.1. AC015985 Genomic DNA. Translation: AAF23253.1. CP002686 Genomic DNA. Translation: AEE74816.1. CP002686 Genomic DNA. Translation: AEE74817.1. AF375451 mRNA. Translation: AAK53035.1. BT033101 mRNA. Translation: ACF16163.1.
IPI	IPI00542446. IPI00545323.
RefSeq	NP_187593.1. NM_111817.3. NP_974269.1. NM_202540.1.
UniGene	At.16947.
3D structure databases
ProteinModelPortal	Q9SF85.
SMR	Q9SF85. Positions 5-344.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SF85.
Proteomic databases
PRIDE	Q9SF85.
ProMEX	Q9SF85.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G09820.1; AT3G09820.1; AT3G09820.
GeneID	820140.
GenomeReviews	Gene locus AT3G09820 in contig BA000014_GR.
KEGG	ath:AT3G09820.
NMPDR	fig|3702.1.peg.13000.
Organism-specific databases
TAIR	At3g09820.
Phylogenomic databases
GeneTree	EPGT00050000007167.
HOGENOM	HBG314716.
InParanoid	Q9SF85.
OMA	SVKIAQW.
PhylomeDB	Q9SF85.
ProtClustDB	PLN02548.
Enzyme and pathway databases
BRENDA	2.7.1.20. 399.
Gene expression databases
ArrayExpress	Q9SF85.
Genevestigator	Q9SF85.
GermOnline	AT3G09820. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001805. Adenokinase. IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. [Graphical view]
KO	K00856.
PANTHER	PTHR10584:SF24. Adenokinase. 1 hit.
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PRINTS	PR00989. ADENOKINASE.
PROSITE	PS00583. PFKB_KINASES_1. False negative. PS00584. PFKB_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADK1_ARATH

Accession

Primary (citable) accession number: Q9SF85
Secondary accession number(s): B3LFB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program