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Protein

Phosphatidate phosphatase PAH1

Gene

PAH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Magnesium-dependent phosphatidate phosphatase which catalyzes the dephosphorylation of phosphatidate to yield diacylglycerol. Acts redundantly with PAH2 to repress phospholipid biosynthesis at the endoplasmic reticulum (ER). May function indirectly as repressor of multiple enzymes involved in phospholipid biosynthesis. Is involved in the pathway of galactolipid synthesis in the ER, which is required for the membrane lipid remodeling, an essential adaptation mechanism to cope with phosphate starvation.3 Publications

Catalytic activityi

A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.

Cofactori

Mg2+2 Publications

pH dependencei

Optimum pH is 6.5.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to phosphate starvation Source: TAIR
  • galactolipid biosynthetic process Source: TAIR
  • intracellular protein transport Source: UniProtKB
  • lipid metabolic process Source: TAIR
  • phospholipid biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:GQT-2137-MONOMER.
ARA:GQT-2138-MONOMER.
ReactomeiR-ATH-1483191. Synthesis of PC.
R-ATH-1483213. Synthesis of PE.
R-ATH-4419969. Depolymerisation of the Nuclear Lamina.
R-ATH-75109. Triglyceride Biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidate phosphatase PAH1 (EC:3.1.3.4)
Alternative name(s):
Phosphatidic acid phosphohydrolase 1
Short name:
AtPAH1
Gene namesi
Name:PAH1
Ordered Locus Names:At3g09560
ORF Names:F11F8.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G09560.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB
  • protein storage vacuole membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but the double mutants pah1 and pah2-1 show reduced growth.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi83 – 831G → A: Reduces activity 3-fold. 1 Publication
Mutagenesisi707 – 7071D → A: Loss of activity. 1 Publication
Mutagenesisi709 – 7091D → A: Loss of activity. 1 Publication
Mutagenesisi752 – 7521S → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 904904Phosphatidate phosphatase PAH1PRO_0000425528Add
BLAST

Proteomic databases

PaxDbiQ9SF47.
PRIDEiQ9SF47.
ProMEXiQ9SF47.

PTM databases

iPTMnetiQ9SF47.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, flowers, siliques, embryos and mature seeds.2 Publications

Gene expression databases

GenevisibleiQ9SF47. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G09560.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SF47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 112112N-LIPAdd
BLAST
Regioni703 – 857155C-LIPAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi707 – 7115DXDXT motif

Domaini

Sequence similaritiesi

Belongs to the lipin family.Curated

Phylogenomic databases

eggNOGiKOG2116. Eukaryota.
COG5083. LUCA.
HOGENOMiHOG000153456.
InParanoidiQ9SF47.
KOiK15728.
OMAiSECKDEQ.
OrthoDBiEOG093601ZR.
PhylomeDBiQ9SF47.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR031703. Lipin_mid.
IPR007651. Lipin_N.
IPR013209. LNS2.
IPR031315. LNS2/PITP.
[Graphical view]
PfamiPF16876. Lipin_mid. 1 hit.
PF04571. Lipin_N. 1 hit.
PF08235. LNS2. 1 hit.
[Graphical view]
SMARTiSM00775. LNS2. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SF47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVGRVGSL ISQGVYSVAT PFHPFGGAID VIVVQQQDGS FRSTPWYVRF
60 70 80 90 100
GKFQGVLKGA EKFVRISVNG TEADFHMYLD NSGEAYFIRE VDPAANDTNN
110 120 130 140 150
LISGSENNNG NQNNGVTYRL EHSLSDSGTG ELREGFDPLS RLERTESDCN
160 170 180 190 200
RRFYDFQDDP PSPTSEYGSA RFDNLNVESY GDSQGSDSEV VLVSIDGHIL
210 220 230 240 250
TAPVSVAEQE AENLRLNTPQ FHLAPGDGTE FCEGNTEFAS SETPWDTEYI
260 270 280 290 300
DKVEESSDTA NIASDKVDAI NDERNDLDSH SRDNAEKDSH DAERDLLGSC
310 320 330 340 350
LEQSELTKTS ENVKSEEPGP TFEDRNLKEG EFPLRTIMEN DRSEDEVTIE
360 370 380 390 400
SIDTLVDSFE SSTTQITIEE VKTTEGSRIS VDSNADSECK DEQTSAETAI
410 420 430 440 450
LFNNQESSIS VDSNADSECK DEQPRISAET AILINNQEGG IIESEDQDSE
460 470 480 490 500
RVSIDSTREE VDKDNEDRKT VVSVGVTSSV DEGEPDTDQR YELSLCKDEL
510 520 530 540 550
RQGMGLSAAA EVFDAHMISK EEYINSATSI LESENLVVRI RETYMPWTKA
560 570 580 590 600
ARIVLGKAVF DLDLDIQPDD VISVEENESP KPKDDETTIT PSSSGTRWRL
610 620 630 640 650
WPIPFRRVKT VEHTGSNSSS EEDLFVDSEP GLQNSPETQS TTESRHESPR
660 670 680 690 700
RQLVRTNVPT NEQIASLNLK DGQNMITFSF STRVLGTQQV DAHIYRWRWD
710 720 730 740 750
TKIVISDVDG TITKSDVLGQ FMPFIGKDWT QSGVAKLFSA IKENGYQLLF
760 770 780 790 800
LSARAIVQAY LTRNFLNNLK QDGKALPTGP VVISPDGLFP ALYREVIRRA
810 820 830 840 850
PHEFKIACLE DIRKLFPTDY NPFYAGFGNR DTDELSYRKL GIPKGKIFII
860 870 880 890 900
NPKGEVATGH RIDVKKSYTS LHTLVNDMFP PTSLVEQEDY NPWNFWKLPI

EEVE
Length:904
Mass (Da):101,003
Last modified:May 1, 2000 - v1
Checksum:iA1E9469E71788549
GO

Sequence cautioni

The sequence ACT37431 differs from that shown.Sequencing errors.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti663 – 6631Q → R in BAF01347 (Ref. 4) Curated
Sequence conflicti884 – 8841L → F in BAF01347 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ591133 mRNA. Translation: ACT37431.1. Sequence problems.
AC016661 Genomic DNA. Translation: AAF23287.1.
CP002686 Genomic DNA. Translation: AEE74780.1.
CP002686 Genomic DNA. Translation: AEE74781.1.
CP002686 Genomic DNA. Translation: AEE74782.1.
AK229489 mRNA. Translation: BAF01347.1.
RefSeqiNP_001118604.1. NM_001125132.1.
NP_187567.1. NM_111790.3.
NP_974268.1. NM_202539.1.
UniGeneiAt.18994.

Genome annotation databases

EnsemblPlantsiAT3G09560.1; AT3G09560.1; AT3G09560.
AT3G09560.2; AT3G09560.2; AT3G09560.
AT3G09560.3; AT3G09560.3; AT3G09560.
GeneIDi820113.
GrameneiAT3G09560.1; AT3G09560.1; AT3G09560.
AT3G09560.2; AT3G09560.2; AT3G09560.
AT3G09560.3; AT3G09560.3; AT3G09560.
KEGGiath:AT3G09560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ591133 mRNA. Translation: ACT37431.1. Sequence problems.
AC016661 Genomic DNA. Translation: AAF23287.1.
CP002686 Genomic DNA. Translation: AEE74780.1.
CP002686 Genomic DNA. Translation: AEE74781.1.
CP002686 Genomic DNA. Translation: AEE74782.1.
AK229489 mRNA. Translation: BAF01347.1.
RefSeqiNP_001118604.1. NM_001125132.1.
NP_187567.1. NM_111790.3.
NP_974268.1. NM_202539.1.
UniGeneiAt.18994.

3D structure databases

ProteinModelPortaliQ9SF47.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G09560.1.

PTM databases

iPTMnetiQ9SF47.

Proteomic databases

PaxDbiQ9SF47.
PRIDEiQ9SF47.
ProMEXiQ9SF47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G09560.1; AT3G09560.1; AT3G09560.
AT3G09560.2; AT3G09560.2; AT3G09560.
AT3G09560.3; AT3G09560.3; AT3G09560.
GeneIDi820113.
GrameneiAT3G09560.1; AT3G09560.1; AT3G09560.
AT3G09560.2; AT3G09560.2; AT3G09560.
AT3G09560.3; AT3G09560.3; AT3G09560.
KEGGiath:AT3G09560.

Organism-specific databases

TAIRiAT3G09560.

Phylogenomic databases

eggNOGiKOG2116. Eukaryota.
COG5083. LUCA.
HOGENOMiHOG000153456.
InParanoidiQ9SF47.
KOiK15728.
OMAiSECKDEQ.
OrthoDBiEOG093601ZR.
PhylomeDBiQ9SF47.

Enzyme and pathway databases

BioCyciARA:GQT-2137-MONOMER.
ARA:GQT-2138-MONOMER.
ReactomeiR-ATH-1483191. Synthesis of PC.
R-ATH-1483213. Synthesis of PE.
R-ATH-4419969. Depolymerisation of the Nuclear Lamina.
R-ATH-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ9SF47.

Gene expression databases

GenevisibleiQ9SF47. AT.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR031703. Lipin_mid.
IPR007651. Lipin_N.
IPR013209. LNS2.
IPR031315. LNS2/PITP.
[Graphical view]
PfamiPF16876. Lipin_mid. 1 hit.
PF04571. Lipin_N. 1 hit.
PF08235. LNS2. 1 hit.
[Graphical view]
SMARTiSM00775. LNS2. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAH1_ARATH
AccessioniPrimary (citable) accession number: Q9SF47
Secondary accession number(s): D8L210, Q0WNF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.